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Effect of Packaging Methods on Postharvest Quality of $Tah$ $Tasai$ Chinese Cabbage ($Brassica$ $campestris$ var. $narinosa$) Baby Leaf Vegetable (어린잎 채소 다채의 포장방법이 품질에 미치는 영향)

  • Lee, Jung-Soo;Lee, Youn-Suk
    • Food Science and Preservation
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    • v.19 no.1
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    • pp.1-6
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    • 2012
  • The effect of the packing methods for enhancing the shelf life and improving the postharvest quality of the $tah$ $tasai$ Chinese cabbage baby leaf vegetable was studied during storage. Fresh baby leaf vegetables were packed in four commercial packaging types: (1) a non-perforated bag with a 0.03-mm oriented polypropylene (OPP) film; (2) a perforated bag with 1.0-mm-diameter holes on an OPP film; (3) a 0.40-mm polyethylene terephthalate (PET) container with a hinged lid; and (4) an expanded polystyrene (EPS) tray wrapped with a 0.02-mm polyvinyl chloride (PVC) film. The quality parameters, such as the weight loss, moisture content change, color difference, and appearance of the baby leaf vegetables were investigated. The baby leaf vegetables in the PET container and in the non-perforated OPP film bag showed relatively low weight loss, high moisture content, and good external appearance compared to those in the EPS tray and in the perforated OPP film bag during limited storage periods, at $16^{\circ}C$. The PET container also protected the baby leaf vegetables from physical damage. The study results will enable the selection of a better packaging system for extending the freshness and increasing the market ability of baby leaf vegetables.

Investigation of the Growth Rate Change in Recombinant BCG which was cloned Mycobacterium tuberculosis Adenylate Kinase Mutation Gene or Human Muscle-type Adenylate Kinase Synthetic Gene (결핵균 Adenylate Kinase 돌연변이 유전자와 Human Muscle-type Adenylate Kinase 합성 유전자를 형질전환한 BCG의 성장속도 변화 유무 조사)

  • Lee, Seung-Heon;Kim, Hyo-Joon;Park, Young-Kil;Bai, Gill-Han
    • Tuberculosis and Respiratory Diseases
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    • v.60 no.2
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    • pp.187-193
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    • 2006
  • Background : Normal cell proliferation and viability is strongly depends on the availability of metabolic energy and the maintenance of the appropriate adenylate-nucleotide pools. Hypothetically, changes in adenylate kinase (AK) expression could therefore be associated with adaptation to altered growth characteristics or inversely altered growth characteristics of proliferating cells could drive the changes in the metabolic profile. This study investigated whether the expression of either AK1 or a Mycobacterium tuberculosis adenylate kinase mutant which has the same catalytic activity of AK1 could affect the growth rate of slow-growing BCG. Method : Recombinant BCGs, which were cloned the human muscle-type adenylate kinase synthetic gene (AK1) and adenylate kinase mutation gene (AKmtDM) of Mycobacterium tuberculosis into the Mycobacterium/E.coli expression vectors, were constructed. Recombinant BCGs and wild-type BCG were cultured in 7H9 media and the optical density at 600nm was measured at intervals of 2-3 days. Result : There wasn't the growth rate change induced by AK1 or AKmtDM expression in recombinant BCGs. Conclusion : The expression of AK1 or Mycobacterium tuberculosis adenylate kinase mutant in BCG does not affect the growth rate of BCG.

Development of Suitable Alternative Substrates in Hydroponics of Sweet Pepper (착색단고추의 수경재배에 적합한 대체 배지 개발)

  • Bae, Jong-Hyang;Lee, Yong-Beom;Kim, Ho-Cheol;Cha, Seung-Hoon;Lee, Hye-Jin
    • Journal of Bio-Environment Control
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    • v.17 no.2
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    • pp.138-142
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    • 2008
  • To develop suitable alternative substrates in hydroponics of sweet pepper, changes of water contents of substrates and electric conductivity (EC) of drainage nutrient solution, growth and fruit characteristics according to the kind of coir substrates were investigated compared with rockwool. In all coir substrates compared with rockwool during growing period, water contents were higher, EC of drainage nutrient solution except for fiber 50% were lower, and the coefficient of variation were a little, especially were so in fiber 30% of coir substrates. Plant growth in fiber 30% substrate was better than others but there was no significant difference. Photosynthesis rate and chlorophyll content were best in fiber 50% substrate. Fruit weight was no significant difference, but LID ratio and shape of the fruits were good in fiber 50% substrate and rockwool as which were close to regular square. Therefore, it was estimated that mixed coir substrates with fiber $30{\sim}50%$ are enough in possibility as alternative substrate.

Four Human Cases of Diphyllobothrium nihonkaiense (Eucestoda: Diphyllobothriidae) in China with a Brief Review of Chinese Cases

  • Cai, Yu-Chun;Chen, Shao-Hong;Yamasaki, Hiroshi;Chen, Jia-Xu;Lu, Yan;Zhang, Yong-Nian;Li, Hao;Ai, Lin;Chen, Hai-Ning
    • Parasites, Hosts and Diseases
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    • v.55 no.3
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    • pp.319-325
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    • 2017
  • We described 4 human infection cases of zoonotic fish-tapeworm, Diphyllobothrium nihonkaiense, identified with morphological and molecular characters and briefly reviewed Chinese cases in consideration of it as an emerging parasitic disease in China. The scolex and mature and gravid proglottids of some cases were seen, a rosette-shaped uterus was observed in the middle of the mature and gravid proglottids, and the diphyllobothriid eggs were yellowish-brown in color and displayed a small knob or abopercular protuberance on the opposite end of a lid-like opening. The average size of the eggs was recorded as $62-67{\times}42-45{\mu}m$. The parasitic materials gathered from 4 human cases were morphologically identified as belonging to the genera Diphyllobothrium and Adenocephalus. The phylogenetic analysis based on the nucleotide sequences of cytochrome c oxidase subunit 1 gene of the etiologic agents confirmed that the 4 cases were D. nihonkaiense infection. The finding of 4 additional D. nihonkaiense cases suggests that D. nihonkaiense might be a major causative species of human diphyllobothriasis in China. A combined morphological and molecular analysis is the main method to confirm D. nihonkaiense infection.

Studies on the aeration improvement of inner bottle culture system during the mycelial culture of Flammulina velutipes (팽나무버섯의 균사배양 중 배양기 내부 통기성 개선)

  • Shim, Kyu-Kwang;Yoo, Young-Jin;Koo, Chang-Duck;Kim, Young-Seok;Kim, Myung-Koon
    • Journal of Mushroom
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    • v.10 no.1
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    • pp.15-20
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    • 2012
  • Ventilation effects of bottles($1,100m{\ell}$) for culturing Flammulina velutipes on its mycelial growth and mushroom production were investigated. The degree of ventilation were controlled with hole positions, upper and under, and hole sizes in the bottle lids. The ventilation effects were measured with the contents of carbon dioxide, free sugars, chitin, moisture in the bottles and with the amount of produced mushrooms from the bottles. Carbon dioxide concentrations within the culturing bottles at exponential mycelial growth period vertex were relatively high in the bottles with lids without both a sponge and an aeration hole, and in those with a smaller hole. Free sugar contents in the mycelia were the highest in those with a 47mm hole on both sides, and in those with 26~33mm holes only underside. Chitin content was the highest in those with a 26mm hole only underside. On the other hand, the lids with 42mm~47mm holes on the both sides greatly lost water and decreased the mushroom production. In conclusion, the most efficient ventilation hole sizes on the lids for bottle($1,100m{\ell}$) cultivation of Flammulina velutipes using $1,100m{\ell}$ polypropylene bottle were 19mm on both sides of the lid and 26mm on only underside. They produced more mushrooms than the control by 6~9 %.

In a Universal Design Viewpoint Usability Evaluation Research to PET Bottle (유니버설 디자인 관점에서의 PET BOTTLE에 대한 사용성 평가 연구 -어린이 음료용기를 중심으로-)

  • Kim Sang-Sik
    • The Journal of the Korea Contents Association
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    • v.5 no.5
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    • pp.124-132
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    • 2005
  • While use of a pet bottle with use it is light recently and convenient increases quickly, it is used from the child, also diffusing the user to a pet bottle to people of various classes, such as an old person, a woman, a male, the man in the street, and a disabled person. However, those who are standardized on the basis of the common man with the plan energy of a pet bottle, and cannot attain to the standard, That is, a child and a disabled person may be condemnedto the vague situation that a lid cannot be opened or a contents thing cannot be drunk without external help. And the characteristic of a material and production which are called PET Even if convenience is emphasized for the cost reduction of COST, a design is chosen easily, and even if it calls it the man in the street, the point of coming to experience a situation with the difficult time of use can be found well. In research of a book, problem derivation which classifies entering school and a preschool boy and girl child for a child drink for an object first by the viewpoint of universal design paying attention to such problems, and leads the actual use example of a pet bottle was carried out in true heart, and various notional approach was shown by the solution method.

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Addition Effect of Seed-associated or Free Linseed Oil on the Formation of cis-9, trans-11 Conjugated Linoleic Acid and Octadecenoic Acid by Ruminal Bacteria In Vitro

  • Wang, J.H.;Song, M.K.;Son, Y.S.;Chang, M.B.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.8
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    • pp.1115-1120
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    • 2002
  • The effects of seed-associated or free linseed oil on fermentation characteristics and long-chain unsaturated fatty acids composition, especially the formation of conjugated linoleic acid (CLA) and octadecenoic acid (trans-11 $C_{18:1}$, $t-C_{18:1}$) by mixed ruminal bacteria were examined in vitro. Concentrate (1% of culture solution, w/v, as-fed basis) with ground linseed (0.6% of culture solution, w/v, DM basis) or linseed oil as absorbed onto ground alfalfa hay was added to 600 ml mixed solution consisting of strained rumen fluid and artificial saliva at the ratio of 1:1 in a glass culture jar. The culture jar was covered with a glass lid with stirrer, and placed into a water-bath ($39^{\circ}C$) and incubated anaerobically up to 24 h. Seed-associated or free linseed oil did not significantly affect the pH and ammonia concentration in the culture solution. Molar percent of acetate tended to increase while that of propionate decreased with the addition of free oil treatment throughout the incubation. Differences in bacterial number were relatively small, regardless of the form of supplements. Decreasing trends in the compositions of linoleic acid ($C_{18:2}$) and linolenic acid ($C_{18:3}$) but increasing trends of stearic acid ($C_{18:0}$), $t-C_{18:1}$ and CLA compositions were found from culture contents up to 12h incubation when incubated with both ground linseed and linseed oil. The compositions of $C_{18:0}$, $C_{18:2}$ and $C_{18:3}$ were greater but those of oleic acid ($C_{18:1}$), $t-C_{18:1}$ and CLA were smaller in a culture solution containing ground linseed than those containing linseed oil. The ratio of $t-C_{18:1}$ to CLA was lower in the culture solutions containing linseed oil up to 12h incubations as compared to those containing ground linseed.

A Case Study Stormwater Treatment by Channel-Type Wetland Constructed on the Flood Plane of the Stream (하천 고수부지에 설치한 수로형 인공습지에 의한 강우 유출수 처리에 관한 연구)

  • Kim, Piljoo;Han, Euilyung;Kim, Youngchul
    • Journal of Wetlands Research
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    • v.19 no.1
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    • pp.80-89
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    • 2017
  • Researches about NPS(Non-point Pollution Source) reduction have been widely carried out in recent years. A pilot channel-type constructed wetland (wet swale) was constructed in Rongyin area to treat stormwater generated from a green house agro-land of 22.7 ha. From 2006 to 2008, monitoring was conducted to evaluate its performance on the removal effect for organic pollutants as well as nutrients. Totally, sampling trips of 17 rainfall events were made and they covered most types of storm events in Korea. The channel-type constructed wetland have average removal efficiencies of 78.3~92.0%, 56.4~66.1%, 28.2~45.5% and 50.6~66.4% for SS, COD, TN and TP, respectively. According to four methods for estimating the removal efficiency, the average efficiencies of TSS, COD, TN and TP are 86.0%, 60.1%, 30.1% and 53.5%, respectively. From 2006 to 2008, annual efficiency improved due to infiltration potential increase. It was found that most of the pollutants removed in this channel type of wetland was particulate solids bound pollutants, which is assumed fact that it lacks of physico-chemical treatment conditions which are commonly observed in the retention type of constructed wetlands.

Diagnosis of Functional Nasolacrimal Duct Obstruction Using Dacryoscintigraphy (누비공신티그라피를 이용한 기능적 누도폐쇄의 진단)

  • Lim, Hyun-Wook;Sohn, Hyung-Sun;Kim, Euy-Neyng;Chung, Yong-An;Kim, Sung-Hoon;Chung, Soo-Kyo
    • The Korean Journal of Nuclear Medicine
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    • v.34 no.6
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    • pp.508-515
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    • 2000
  • Purpose: To evaluate the value of dacryoscintigraphy in the assessment of patients with a clinical diagnosis of functional nasolacrimal duct obstruction. Materials and Methods: Dacryoscintigraphy was performed in symptomatic 35 lacrimal drainage systems in 18 patients (age range: $37{\sim}76$, 8 males, 10 females) that were patent on syringing. Results: Abnormalities were detected with dacryoscintigraphy in 75.8% of systems. The positive scintigrams were subdivided into those demonstrating prelacrimal sac delay (31.8%), delay at the lacrimal sac/junction (40.9%), or delay within the duct (21%). Conclusion: Dacryoscintigraphy is noninvasive useful technique in the assessment of the functional nasolacrimal duct obstruction and very sensitive at detecting abnormalities in patients with lid laxity caused by senile change and facial nerve palsy.

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Differentiation Trend of Rare Earth Elements of the Skaergaard Intrusion (Skaergaard 암체의 희토류의 분화경향)

  • Yun D. Jang
    • Economic and Environmental Geology
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    • v.34 no.6
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    • pp.617-625
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    • 2001
  • The Skaergaard intrusion is widely considered a type example of a strongly fractionated, layered intrusion that has undergone extensive in situ igneous differentiation. The Intrusion, therefore, should be a good locality for modeling trace element vriation in a closed system. Previous studios (Haskin and Haskin, 1968; Faster et al., 1974), however, have suggested thats the rare earth elements in whole rocks and mineeral separates from the Intrusion did not fellow the expected trend for closed system crystatllization. Trace element modeling using published distribution coefficients, modal abundances of the coexisting minerals, and the concentration of trace elements In whole rocks and mineral separates from the Skaergaard Intrusion, reveals that the rare earth elements were significantly Influenced by the crystallization of abundant apatite in the Layered Series suring the final stages of crystallization. The results of trace element modeling also suggcsts that apatite, which appears sporadically in the UBS, is not a primary liquidus phase in these samples as previously suggested (Naslund, 1984) but an interstitial phase that (lid not directly effect trace element abundances In the evolving magma As the Skaergaard magma coaled convection, or convected as small Isolated cells during the final stages of differentiation, an elebated $P_{H2O}$ Induced by accumulation of volatile elements near the roof of the magma chamber ingibited or delayed the precipitation of primary apatite in the UBS If the Skaergaard differentiation Is modeler assuming primary apatite crystallization In the upper par of the LS where abundant modal apatite is present, and only late stage crystallization of apatite In the UBS where apatite Is less abundant, rare earth elements abundances follow a closed system variation trend. These results rule but any differentiation model for the Skaergaard Intrusion that Includesvolumetrically significant injections or discharges of magma Into or out of the chamber during the final 20% of the crystallization history.

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