• Clinical and Experimental Reproductive Medicine
• /
• v.14 no.2
• /
• pp.138-148
• /
• 1987

5${\alpha}$-dihydrotestosterone(DHT)과 testosterone(T)은 남성 생식기관의 주 생성 호르몬들로 그 구조가 매우 비슷하여 이들 각 개를 특이하게 측정(specific determination)하는 방법이 개발되지 않고 있다. 본 연구는 고속액체 크로마토그라피(HPLC)를 이용하여 이들을 분리한 후 섬광면역측 정법(Luminescence immunoassay, LIA)으로 정량하는 방법을 개발하여 이들의 응용 가능성을 검토하고져 하였다. DHT와 T의 retention time은 각각 10.3min, 17.6min이었다. DHT-LIA와 T-LIA에 서 다른 스테로이드들과의 교차반응도는 방사면역측정법(RIA)과 대동소이하였다. 정도관리(quality control) 시료의 intra-assay variation은 DHT-LIA가 8.7%, T-LIA가 6.0%의 변이계수를 나타내었고, inter-assay variation의 변이계수는 각각 12.0% 및 15.3%이었다. 실측치(y)와 기대치(x)간의 관계를 보면, DHT-LIA경우는 Y=0.94X+0.9(r=0.989), T-LIA는 Y=1.01X+0.06(r =0.988)로 두 측정치 사이에는 통계적으로 유의한 차이가 없었다. 위의-측정방법을 이용하여 DHT-enanthate와 T-enanthate 처리후 혈청내 DHT 및 T의 농도변화를 조사한 실험결과 LIA와 RIA의 값사이에 유의한 차이가 없었다. 위의 결과로 보아 본 실험에서 개발된 DHT와 T의 섬광면역측정법은 정립되었다고 사려된다.

• 한국정보통신설비학회:학술대회논문집
• /
• 2008.08a
• /
• pp.131-136
• /
• 2008

System을 통해 출력되는 신호를 측정할 때 정확한 측정을 방해하는 요소로 잡음이 있다. 이런 신호 측정의 방해 요소인 잡음을 제거 하는 방법 중의 하나로 Lock-in Amp(LIA)가 사용되고 있다. 본 논문에서는 잡음 신호의 제거를 위해 사용 하는 LIA를 제작 하고 특성을 파악 하였으며 RF통신을 이용하여 무선 형태로 제작 하였다. 현재 상용화된 LIA는 프로브를 통한 유선으로 측정신호의 입력을 받게 되지만 본 논문에서 제작된 LIA는 무선신호 형태로 입력 하게 된다. RF통신의 케리어 주파수는 447.9[MHz]로 Digital GMSK 변복조방식을 이용하였다. LIA의 제작은 Dual Phase Sensitive Detecter을 사용하였으며, 주요 구성 요소인 Phase Locked Loop, Phase Shifter, Phase Sensitive Detector, Low Pass Filter등의 구조와 특성을 조사하였다.

• Journal of the Korean Institute of Landscape Architecture
• /
• v.27 no.5
• /
• pp.66-79
• /
• 2000

Landscape impact assessment(LIA) is an aid to decision-making. For the decision maker, LIA provides a scientific examination of the predicted landscape implications of a proposed action and of the mitigation measures, before a decision is taken. Thus, the efficacy of LIA depends on the scientific credibility of the prediction and mitigation measures described in the landscape impact statement. The purpose of this paper was to examine the efficacy of LIA through the content analysis of prediction and mitigation measures in the selected 39 landscape impact statements and 34 environmental impact statements. The content of the selected statements was analyzed in terms of the quantification, precision, significance, and likelihood. The results showed that both prediction and mitigation measures were very low in the scientific credibility. Specifically, the prediction was ambiguously described without scientific probability, and the effect of mitigation measures was not specifically presented in the reports. Thus, landscape impact statement can not give credible information in the decision making process, which weakens the efficacy as an aid to decision-making. Based on the results, several suggestions were presented to enhance the efficacy of LIA.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
• /
• v.31 no.5
• /
• pp.343-353
• /
• 2013

Recently, the landscape change by development has become more important and it's been stipulated that environmental effects evaluation must include LIA which reflects the said issue. Even though the selection of viewpoints which have the biggest impact on LIA result is stipulated in accordance with related rules, the criteria are inconsistent. Therefore, LIA experts' or project developers' subjective opinions have been very influential. In this case, wrong viewpoints which do not meet the selection criteria could be selected based on a discretionary interpretation on them, which could in turn substantially reduce an accuracy and reliability of the LIA results. Therefore, this research suggested the reverse-viewshed frequency technique as method that can be verified accuracy and reliability of viewpoints. And it has comparatively analyzed effective viewpoints using reverse-viewshed frequency analysis on viewpoints which were selected for LIA. As a result, the average validity was just 58% of total viewpoints used in construction sites. And the validity of viewpoints decreased as viewshed frequency increased. Based on results above, it was able to verify that the unreasonable points of a substantial proportion have been used as viewpoints in LIA process.

• Clinical and Experimental Reproductive Medicine
• /
• v.15 no.2
• /
• pp.83-92
• /
• 1988

혈액에서 생물학적 활성을 나타내는 (bioavailable) steroid hormone은 주로 비결합형(free form)과 알부민 결합형(albumin-bound form)으로 구성된다. 특히 Testosterone (T)과 5 alpha-Dihydrotestosterone (DHT)의 활성적 분획이 전체의 T, DHT 양에 비해 생리적 현상과 보다 잘 일치하는 것으로 알려지고 있다. 본 연구는 섬광면역측정법(Luminescence immunoassay, LIA)으로 혈청내 활성적 T 및 DHT의 농도의 측정에 이용하고져 하였다. 항체는 T- 또는 DHT-3-CMO-BSA를 항원으로 토끼에 면역주사하여 얻었다. 추적자는 T-3-CMO, DHT-3-CMO에 aminobutylethylisoluminol(ABEI)를 부착시켜 사용하였다. 항체중 IgG분획을 Protein-A-Sepharose CL-4B로 분리한 후 Immunobead(Bio-Rad)에 부착시켜 Solid-phase LIA를 실시하였다. 본 연구에서 LIA는 정확도(accuracy), 정밀도(precision), 감도(sensitivity), 교차반응도(specificity)등을 조사하고, 기존의 방사면역측정법(RIA)과 비교하여 만족할만한 결과를 얻었다. 혈청내 T및 DHT의 활성적 분획의 농도를 측정한 결과는 다음과 같았다. T의 경우는 남성에서 T의 전체량의 33% 이상으로 $7.1{\pm}1.5nmol/l$, 여성에서는 26% 이상으로 $0.28{\pm}0.05nmol/l$이었다. DHT의 활성적 분획은 남성의 경우 $601.7{\pm}85.8pmol/l$, 여성의경우 $52.4{\pm}19.9\;pmol/l$이었다. 이상의 결과를 보아 본 연구에서 이용된 LIA는 혈청내 활성적 농도를 측정하기에 충분하다고 사료된다. 또한 이 방법을 이용하여 여성의 Androgenicity 및 남성 정소기능등의 제어방법에 응용될 수 있을 것으로 판단된다.

• Korean Journal of Radiology
• /
• v.23 no.4
• /
• pp.479-487
• /
• 2022

Objective: To prospectively evaluate the efficacy of lauromacrogol injection for ablation (LIA) of benign predominantly cystic thyroid nodules and its related factors. Materials and Methods: A total of 142 benign predominantly cystic thyroid nodules (median volume, 12.5 mL; range, 0.4-156 mL) in 137 patients (male:female sex ratio, 36:101; mean age ± standard deviation [SD], 49 ± 13 years) were treated with LIA after being confirmed as benign via cytology. The volume reduction rate (VRR) of the nodules and cosmetic score were evaluated during follow-up at 1, 3, and 6 months after treatment and every 6 months thereafter. A VRR of ≥ 50% at the 12-month follow-up was considered to indicate effective treatment. The associations between the clinical factors and nodular ultrasound features, including the initial nodule volume, proportion of solid components, vascularity grade and ineffective treatment (VRR of < 50% at the 12-month follow-up), and regrowth were analyzed. Results: All patients completed follow-up for at least 12 months. The average ± SD follow-up period was 32 ± 11 months (range, 12-54 months). The effective treatment rate was 73.2% (104/142), while the regrowth rate was 12.0% (17/142) at the last follow-up. Grade 2-3 intranodular vascularity in the solid components of the nodules was the only independent factor associated with ineffective treatment, with an odds ratio (reference category, grade 0-1) of 3.054 (95% confidence interval, 1.148-8.127) (p = 0.025). Conclusion: LIA is an effective treatment for predominantly cystic thyroid nodules. Grade 2-3 intranodular vascularity in the solid components of nodules is the only independent risk factor for ineffective LIA.

• Journal of Ecology and Environment
• /
• v.29 no.3
• /
• pp.287-293
• /
• 2006

We carried out pollen analysis and radiocarbon dating from sediments of Wangdeungjae moor (960 m a.s.l.) to clarify vegetation history of temperate broad-leaved deciduous forest zone of Mt. Jiri. Three local pollen zones and two sub-zones were recognized on the basis of variation in the palynofloral assemblage: I. Quercus zone (150-114 cm), II. Quercus-Pinus zone (114-43 cm): IIa. Quercus-Pinus subzone (114-83 cm), IIb. Quercus-Pinus-Herbs subzone (83-43 cm), III. Pinus-Quercus zone (43-27 cm). Radiocarbon date from sediment depth 111-116 cm was estimated $1,160{\pm}40yr$ B.P. From these results, overall vegetation around of Wangdeungjae moor and Mt. Jiri have changed from Q. mongolica dominant to steady decrease of Q. mongolica whereas steady increase of P. densiflora, finally abrupt increase of P. densiflora. 'Medieval Warm Period (MWP)' and 'Little Ice Age (LIA)' have not had an effect on change of vegetation around, at least, Mt. Jiri. But at maximum period of LIA, extension of grassland and steady increase of Pinus were recognized around the moor. After then, an abrupt increase of Pinus is supposed that vegetation change is reflected in human impact surging around foothills.

• Proceedings of the KIEE Conference
• /
• 2008.07a
• /
• pp.1771-1772
• /
• 2008

전동기나 발전기와 같은 회전기기에 주로 발생하는 주기외란은 시스템 성능을 저하시키는 특성으로서 고급제어시스템 구현을 위하여 반드시 보상되어야 한다. 본 논문은 신호처리기법의 일종인 Lock In Amplifier(LIA) 알고리즘 기반 외란보상 제어기를 제안한다. 제안하는 제어규칙은 공칭제어기와 보조제어기로 구성되며 전자는 외란을 고려하지 않은 시스템 모델에 대하여 상태궤환 제어기법으로 산출되며 후자는 LIA 기법을 이용하여 외란특성을 실시간으로 추정하여 연산되어진다. 제안하는 제어시스템은 기존의 결정적 외란으로부터 발생되었던 실시간 제어오차를 월등히 개선하는 장점을 가지고 있다. 실시간 전동기 제어장치를 통해 제안하는 알고리즘의 성능의 우수성 및 타당성을 검증한다.

• Journal of Microbiology and Biotechnology
• /
• v.13 no.4
• /
• pp.509-516
• /
• 2003

A model system for the immnunochemical detection of Escherichia coli O157:H7 using a combined immunomagnetic separation (IMS) and test-strip liposome immunoassay (LIA) procedure was developed. Immunomagnetic beads coated with anti-E. coli O157 IgG antibodies were used to separate the E. coli O157 (including the H7 serotype) from culture. Immunoliposomes, whose surface was conjugated to goat anti-E. coli O157:H7 IgG and which encapsulated the marker dye, sulforhodamine B, were used as a detection label. The test strip, onto which antibodies to goat IgG were immobilized, was the immunosensor capturing immunoliposomes that did not bind to E. coli O157:H7 on the immunomagnetic bead-E. coli O157:H7 complexes. In experiments, pure cell culture suspensions of $10^5 E.$ coli O157:H7 organisms per ml produced a measurable signal inhibition, whereas a weak yet detectable signal inhibition occurred with $10^3CFU/ml$. The inhibition signals increased, when the incubation time for IMS was extended to 90 min and higher IgG-tag density (0.4mol%) was used on the liposomes. With 0.2 and 0.4mol% IgG-tagged liposomes, the IMS-LIA procedure showed more improved signal inhibitions than those of a direct (no IMS) LIA. The combined assay, which measures the instantaneous signal from immunoliposomes, can be completed within 90 min, making it significantly faster than conventional plating methods and enzyme-linked immunosorbent assay (ELISA). Accordingly, it is quite feasible to use the combined immunoassay format of IMS and dye-loaded immunoliposomes for the detection of E. coli O157:H7.

• Journal of Chest Surgery
• /
• v.55 no.3
• /
• pp.252-254
• /
• 2022