• The Korean Journal of Zoology
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• v.32 no.3
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• pp.275-280
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• 1989

Present study examined the endogenous release of luteinizing hormone releasing hormone (LHRH) from superiused hypothalamic sBces derived from Korean native cattie (KNC). In addition, the in vitro secretory pattern of LHRH release in '(NC was compared with that in imported cattle such as Holstein cow. The median eminences (ME) of hypothalamic tissues were disseded out, sliced and quici'ly placed in ice-cold superfilsion chamber. Superhision chambers containing ME slices were maintained in a constant temperature water-bath at 37$^{\circ}C$. Effluents were colleded on ice at 10 min intervals for a 4 hr superfusion period, and kept -2$0^{\circ}C$ prior to LHRH radloimmunoassay. LHRH release was analyzed by the PULSAR algorithm. The spontaneous release of LHRH from both cows was episodic during a 4 hr superhision period. The mean LHRH release, pulse amplitude and pulse interval m KNC were 11.08 $\pm$ 1.50 pg/min/mg x 10-$^2$, 21.43 1 7.28 pg/mg x 10-$^2$, and 39.42 $\pm$ 3.08 min, which were quite similar to those observed in Holstein cows. The basic charaderistics of the LHRH pulse generator of '(NC appears important for a better understanding about the endocrine function of KNC.