• The Korean Journal of Physiology and Pharmacology
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• 제7권6호
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• pp.349-355
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• 2003

We previously shown that LES contraction depends on $M_3$ receptors linked to PTX insensitive $G_q$ protein and activation of PLC. This results in production of $IP_3$, which mediates calcium release, and contraction through a CaM dependent pathway. In the esophagus ACh activates $M_2$ receptors linked to PTX sensitive $G_{i3}$ protein, resulting in activation of PLD, presumably, production of DAG. We investigated the role of PLC isozymes which can be activated by $G_q$ or $G{\beta}$ protein on ACh-induced contraction in LES and esophagus. Immunoblot analysis showed the presence of 3 types of PLC isozymes, $PLC-{\beta}1$, $PLC-{\beta}3$, and $PLC-{\gamma}1$, but not $PLC-{\beta}2$, $PLC-{\beta}4$, $PLC-{\gamma}2$, $PLC-{\delta}1$, and $PLC-{\delta}2$ from both LES and esophageal muscle. ACh produced contraction in a dose dependent manner in LES and esophageal muscle cells obtained by enzymatic digestion with collagenase. $PLC-{\beta}1$ or $PLC-{\beta}3$ antibody incubation reduced contraction in response to ACh in LES but not in esophageal permeabilized cells, but $PLC-{\gamma}1$ antibody incubation did not have an inhibitory effect. The inhibition by $PLC-{\beta}1$ or $PLC-{\beta}3$ antibody on Ach-induced contraction was antibody concentration dependent. The combination with $PLC-{\beta}_1$ and $PLC-{\beta}_3$ antibody completely abolished the contraction, suggesting that $PLC-{\beta}1$ and $PLC-{\beta}3$ have a synergism to inhibit the contraction in LES. $PLC-{\beta}1$, -${\beta}3$ or -${\gamma}1$ antibody did not reduce the contraction of LES cells in response to DAG ($10^{-6}$ M), suggesting that this isozyme of PLC may not activate PKC. When $G_{q/11}$ antibody was incubated, the inhibitory effect of the incubation of PLC ${\beta}3$, but not of PLC ${\beta}_1$ was additive (Fig. 6). In contrast, when $G_{\beta}$ antibody was incubated, the inhibitory effect of the incubation of PLC ${\beta}_1$, but not of PLC ${\beta}_3$ was additive. This data suggest that $G_{q/11}$/11 or $G{\beta}$ may activate cooperatively different PLC isozyme, $PLC{\beta}_1$ or $PLC{\beta}_3$ respectively.

• Wind and Structures
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• 제24권1호
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• pp.1-24
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• 2017

Wind environment in urban residential areas is an important index to consider when evaluating the living environment. However, due to the complexity of the flow field in residential areas, it is difficult to specify the correct inflow boundary conditions in the large eddy simulation (LES). In this paper, the weighted amplitude wave superposition (WAWS) is adopted to simulate the fluctuating velocity data, which satisfies the desired target wind field. The fluctuating velocity data are given to the inlet boundary of the LES by developing an UDF script, which is implemented into the FLUENT. Then, two numerical models - the empty numerical wind tunnel model and the numerical wind tunnel model with spires and roughness elements are established based on the wind tunnel experiment to verify the present method. Finally, the turbulence generation approach presented in this paper is used to carry out a numerical simulation on the wind environment in an urban residential area in Lisbon. The computational results are compared with the wind tunnel experimental data, showing that the numerical results in the LES have a good agreement with the experimental results, and the simulated flow field with the inlet fluctuations can generate a reasonable turbulent wind field. It also shows that strong wind velocities and turbulent kinetic energy occur at the passageways, which may affect the comfort of people in the residential neighborhood, and the small wind velocities and vortexes appear at the leeward corners of buildings, which may affect the spreading of the pollutants.

• 항공우주시스템공학회지
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• 제13권6호
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• pp.1-8
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• 2019

아음속 유동이 흐르는 후향 계단에서, 입구 경계면에서의 난류를 모사하는 3가지 기법을 조사하였다. 입구 경계면으로 유입되는 평균 유동장과 레이놀즈 응력 프로파일은 실험에서 측정된 결과를 사용하였으며, 입구 경계면의 난류 유동 모사기법으로 synthetic eddy method(SEM), 무작위 변동(random noise) 그리고 균일한 유동 조건(uniform)을 사용하였다. 3차원 유동장의 난류 유동의 모사를 위해 대와동모사(Large Eddy Simulation, LES)를 적용하였다. 3가지 기법에 대한 입구 경계면으로 유입된 유동의 난류 특성과 유동의 재 부착(reattachment) 거리와 속도, 레이놀즈 응력(Reynolds stress)을 비교하였다.

• 한국추진공학회지
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• 제19권1호
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• pp.42-49
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• 2015

3차원 Large Eddy Simulation(LES)와 Proper Orthogonal Decomposition(POD) 기법을 이용하여 고체로켓의 인히비터에서 발생하는 연소실내 압력 진동 특성을 분석하였다. 인히비터 후방에서 발생한 와류는 Flow-acoustic coupling에 의해 주기적으로 반복하여 생성, 소멸이 이루어지는 것을 확인하였고, 이 와류가 내삽 노즐 입구 도출부에 충돌하면서 유동이 불균질하게 분해되고, 후방 돔으로 유입된 유동에 의한 압력 진동은 연소실 압력 진동 가진의 원인이 된다. 또한 인히비터에서 발생하는 와흘림(vortex shedding) 주기는 연소실내 와류 발생 주기와 일치하며, 실험에서 측정된 압력 진동 주파수와 비교 분석하였다.

• 한국전산유체공학회지
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• 제17권3호
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• pp.11-17
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• 2012

In this study, aerodynamic analyses have been conducted for 4-Bladed Vertical-Axis Wind Turbine (VAWT) configuration and the results are compared with experimental data. Reynolds-averaged Navier-Stokes equation with LES turbulence model is solved for unsteady flow problems. In addition, the computation results by standard k-${\omega}$ and SST k-${\omega}$ turbulence models are also presented and compared. An experiment model of 4-Bladed VAWT model has been designed and constructed herein. Experimental tests for aerodynamic performance of the present VAWT model are practically conducted using the vehicle mounted testing system. Comparison results between the experiment and the computational fluid dynamics (CFD) analyses are presented in order to show the accuracy of CFD analyses using the different turbulent models.

• 한국전산유체공학회지
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• 제14권3호
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• pp.115-122
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• 2009

Numerical methods are applied to simulate the smoke behavior in a ventilated tunnel using large eddy simulation (LES) which is incorporated in FDS (Fire Dynamics Simulator) with proper combustion and radiation model. In this study, present numerical results are compared with data obtained from experiments on pool fires in a ventilated tunnel. The model tunnel is $182m(L){\times}5.4m(W){\times}2.4m(H)$. Two fire scenarios with different ventilation rates are considered with two different fire strengths. The present results are analyzed with those from LES without combustion and radiation model and from RANS ($\kappa-\epsilon$) model as well. Temperature distributions caused by fire in tunnel are compared with each other. It is found that thermal stratification and smoke back-layer can be predicted by FDS and the temperature predictions by FDS show better results than LES without combustion and radiation model. The FDS solver, however, failed to predict correct flow pattern when the high ventilation rate is considered in tunnel because of the defects in the tunnel-inlet turbulence and the near-wall turbulence.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2007년도 제28회 춘계학술대회논문집
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• pp.233-237
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• 2007

최근의 실험 결과에 의하면 하이브리드 로켓 연료의 표면에 연소가 진행되지 않은 채 남아있는 점들이 존재함을 확인하였다. 이러한 불규칙적인 spot은 연료의 기화로 인한 분출유동(blowing effect)과 산화제의 유동 사이에서 발생하는 교란에 의한 현상인 것으로 여겨진다. 본 연구에서는 LES를 이용하여 분출이 있을 때 channel 유동을 해석함으로써 연료 표면으로 전달되는 열전달 특성을 해석하였다. 비록 원형 그레인이 아니며 화학반응을 무시하고 열전달을 계사하였으나 근본적으로 연료 표면에서 발생하는 불규칙한 spot의 발생은 작은 크기의 난류 eddy의 존재 때문인 것으로 판단된다.

• 한국전산유체공학회지
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• 제10권3호
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• pp.48-54
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• 2005

Three-dimensional incompressible flow past an open cavity in a channel is investigated using Detached Eddy Simulation(DES). The length to depth ratio of the cavity is 2 and the Reynolds number defined with the cavity depth is 3,360. The DES methods are based on the Menter's SST model. In the present work, two types of inflow conditions are used: one is RANS profile, the other is LES inflow from another Large Eddy Simulation(LES) of fully developed channel flow. The results are compared with experimental data and LES results in terms of the mean statistics, temporal physics and scalar transport phenomenon of the flow.

• 한국연소학회지
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• 제6권2호
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• pp.29-35
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• 2001

Unsteady numerical study has been conducted on combustion dynamics of a lean-premixed swirl-stabilized gas turbine swirl injector. A three-dimensional computation method utilizing the message passing interface (MPI) parallel architecture, large eddy simulation(LES), and proper orthogonal decomposition (POD) technique was applied. The unsteady turbulent flame dynamics are simulated so that the turbulent flame structure can be characterized in detail. It was observed that some fuel lumps escape from the primary combustion zone, and move downstream and consequently produce hot spots. Those flame dynamics coincides with experimental data. In addition, basis modes of the unsteady turbulent flame are characterized using proper orthogonal decomposition (POD) analysis. The flame structure based on odd basis modes is apparently larger than that of even ones. The flame structure can be extracted from the summation of the basis modes and eigenvectors at any moment.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2008년도 제30회 춘계학술대회논문집
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• pp.67-70
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• 2008

스월 연소기의 비반응 난류 유동특성을 파악하기 위하여 3차원 Large Eddy Simulation(LES)을 수행하였다. 연소기는 GEAE LM6000 연소기를 이용하였으며, 실제 실험 결과에 따른 인젝터 유입 형상을 적용하였다. 주 흐름 부분에서 강한 vortex breakdown, 중심 재순환영역, 모서리 재순환영역, 축방향으로 전진하는 스월링 형상, 주기적으로 나타나는 난류 구조를 관찰하였다. 계산된 결과는 실제 실험결과와 선행연구자들의 LES 계산결과와 비교하여 잘 맞음을 확인하였다.