• Journal of Digital Convergence
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• v.14 no.11
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• pp.639-644
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• 2016

The purpose of this study is to determine the effect of the light-emitting-diode (LED) to investigate proliferation of human epidermal keratinocyte and collagen, procollagen expression. In order to determine whether LED irradiation can safely be applied to human skin, the proliferative effects of LED irradiation were determined by MTS assay in Human Epidermal Keratinocytes. Wavelength of 470nm LED irradiation increased mRNA expression of collagen, procollagen without cytotoxity. Our results suggest that 470nm LED irradiation may have a proliferative effects and collagen synthesis property. In order to determine whether LED irradiation can safely be applied to human skin, the cytotoxic effects of LED irradiation were determined by MTS assay in Human Dermal Fibroblasts (HDF). As far as we know, this is the first report demonstrating in vitro collagen synthesis activity of 470nm LED irradiation and being a scientific basis for the cosmetic.

• Journal of Bio-Environment Control
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• v.9 no.4
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• pp.223-229
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• 2000

This study was conducted to analyze the opto-electric characteristics of light-emitting diodes(LED) designed for growth and morphogenesis control of transplant and to quantify the photon flux emittig from LED using a quantum sensor spectroradiometer. Difference in photon flux for blue and red LED between measured by a quantum sensor and measured by a spectroradiometer and numerically integrated was not observed. This result implies a spectroradiometer can be applied to quantify the photon flux emitting from far-red LED, which can not be measured using a quantum sensor. Since photon flux increases in proportion to wavelength, photon flux of LED modules arranged for red and far-red increased in proportion to wavelength, photon flux of LED modules arranged for red and rar-red increased gradually as the number of LED stick emitting far-red in LEd modules increased. Illumination of LED modules arranged for red and far-red decreased as the number of LED stick emitting far-red in LED modules increased. There was no difference in irradiance between LED modules arranged for red and far-red.

• Journal of Applied Biological Chemistry
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• v.62 no.2
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• pp.181-186
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• 2019

The optimum design of LED device for irradiation of 460 nm blue light on extruded rice cake using simulation and the effect of the blue light on the inactivation of Bacillus cereus (B. cereus) group on the rice cake were investigated. The irradiated light intensity patterns on the surface area of the sample were simulated with three different LED arrays (centered, cross, and evenly spaced) and at various distances (22, 32, 42 mm) between the LED modules and the sample. In addition, the uniformity was calculated as Petri factor. The evenly spaced array resulted the most uniform light intensity pattern in the simulation, and the Petri factor of 32 and 42 mm of the distances showed higher than 0.9, which represents the ideal uniformity of LED device. The bacterial population of the rice cake decreased to less than the initial bacterial population during exposure to LED blue light, whereas the bacterial population of the control sample increased. The bacterial count of the rice cake after blue light irradiation for 24 h was 1.21 log CFU/g lower than the control sample. Petri factor increased with increase of the distance between the light source and sample, however, the reduction rate of B. cereus group decreased. Therefore, the design of LED device, that represented the Petri factor higher than 0.9 and inactivated the population of B. cereus group, with evenly spaced and 32 mm of distance between the light source and sample was suitable for extending shelf-life of rice cake.

• Korean Journal of Food Science and Technology
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• v.45 no.4
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• pp.526-529
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• 2013

This study was conducted to evaluate the disinfection effects of Staphylococcus aureus, and Escherichia coli treated with 461-nm LED and pH 5 at $15^{\circ}C$ for 10 h. S. aureus strains were decreased by about 4 log CFU/mL after 461-nm LED irradiation treatment alone for 10 h. E. coli strains were inactivated by irradiation. However, when microorganisms were subjected to a combined treatment of 461-nm LED and pH 5, both strains were inactivated by irradiation for 7 h. The highest D-value was 5.05 h for S. aureus ATCC 27664 and the lowest D-value was 1.39 h for E.coli O157: H7 ATCC 35150 (p<0.05) with 461-nm LED irradiation. For the combined treatment (461-nm LED and pH 5), the highest D-value was 1.58 h for S. aureus ATCC 19095, whereas the lowest D-value was 0.83 h for S. aureus ATCC 27664 (p<0.05). These data showed that bactericidal effects of a combination of pH 5 with 461-nm LED irradiation were enhanced compared to 461-nm LED irradiation alone.

• Korean Journal of Clinical Laboratory Science
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• v.49 no.2
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• pp.150-160
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• 2017

Atopic dermatitis (AD) is a chronic inflammatory skin disease. It is characterized by eczematous lesions, skin dryness, and pruritus. The existing treatment drugs for AD have side effects, especially if the drugs are taken for extended periods. Therefore, new alternative therapies are necessary. The aim of this study was to investigate the combined effects of curcumin administration and LED irradiation on AD. AD-like lesions were induced in BALB/c mice by repeated application of 1-chloro-2,4-dinitrobenzene (DNCB) to the shaved skin of the ear and neck. Thirty male BALB/c mice were divided into five groups: vehicle, DNCB, curcumin, LED, and curcumin+LED groups. Curcumin (0.1 g/kg/day) was administrated repeatedly during a period of 14 days (experimental period) and 630 nm LED irradiation ($5J/cm^2/day$) was performed in the acryl box once a day for 10 days, after inducing AD-like lesions via DNCB application. The severity of AD-like lesions was evaluated during the experimental period, using a modified SCORAD index. Both ear and neck skin tissues were examined histologically for epidermal thickness, mast cell, eosinophil counting, and dermal collagen density. Epidermal cell proliferation and apoptosis were detected using immunohistochemistry and TUNEL, respectively. These were all reduced in SCORAD index, epidermal thickness, collagen density, number of mast cell and eosinophil in dermis, and number of proliferating cell and apoptotic cell in epidermis by curcumin administration and 630 nm LED irradiation. Moreover, all parameters were significantly lower in the curcumin+LED group compared with the curcumin group and LED group. These results suggest that the combined therapy of curcumin and LED is more effective than a single treatment. We recommend that this can be a feasible alternative therapy to manage AD.

• Clean Technology
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• v.16 no.2
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• pp.88-94
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• 2010

The physiological roles of various wavelength of light emitting diode (LED) on ‘Hayward’ kiwifruit experiencing after-ripening were investigated. Various wavelengths from LED light source were irradiated on kiwifruits kept in plastic bags or under open air at $25^{\circ}C$. During two weeks of storage, firmness of Hayward kiwifruits was decreased by $25^{\circ}C$ treatment than by $4^{\circ}C$ treatment. In the $25^{\circ}C$ storage condition, the firmness of kiwifruits was decreased by the treatment of 380 nm UV and 470nm white LED light source. Sweetness of kiwifruits treated with 380 nm UV LED and dark condition at $25^{\circ}C$ increased higher than $15^{\circ}$Brix. The acidity of kiwifruits under open air was decreased 52% by incubating at $25^{\circ}C$ with 660 nm red LED treatment. The acidity of kiwifruits in plastic bags was decreased 52.6, 55.6, 52.8% by the treatment of 440 nm blue, 470 nm white and 660 nm red LED light source, respectively, compared to that of kiwifruits incubated in darkness at $25^{\circ}C$. Decreased acidity irradiated by 660 nm red LED light source can be applied for regulating periods of the kiwifruit after-ripening process. LED light sources emit very narrow wavelength with a power-saving mode, therefore, the usage of these LED light source for regulating the after-ripening process can be classified as a clean biotechnology producing safe and environment-friendly kiwifruits.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2013.10a
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• pp.283-285
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• 2013

피부의 관리를 위한 방법 중 광을 이용한 케어는 비침습적인 방법으로 세포를 활성화 시켜 도움을 주는 매우 유용한 방법이다. 최근에는 낮은 에너지의 LED를 광원으로 이용하는 LLLT (Low Level Light Therapy)가 많이 적용이 된다. 본 논문에서는 피부 미용기기에서 많이 사용되고 있는 가시광 영역의 적색과 황색, 그리고 적외선 영역의 LED를 이용하여 array를 제작하였다. 제작된 LED array는 5V의 전압과 50mA의 전류로 구동되며, 적색이 평균 $10.7mW/cm^2$, 황색이 평균 $1.8mW/cm^2$, 적외선이 평균 $5.5mW/cm^2$의 광출력을 갖는다. 이를 배양된 human cell plate에 각각 10, 20, 40분의 시간으로 조사하였으며, LED array와 조사면 사이에 20 mm의 거리를 두어 균일한 광량이 조사 될 수 있도록 하였다. 실험결과, wound healing의 경우는 적색광을 조사하였을 때, injury line의 길이가 $604.36{\mu}m$에서 10분 조사 시 $368.92{\mu}m$, 20분 조사 시 $298.04{\mu}m$, 40분 조사 시 $491.22{\mu}m$로 줄어드는 효과를 보였으며 특히 20분간 조사하였을 경우의 효과가 가장 좋았다. Cell regeneration의 경우는 황색광을 20분간 조사하였을 경우에 UV 에 의해 손상된 세포 수가 가장 크게 줄어드는 것을 확인할 수 있었다. 결과적으로 photorejuvenation 효과를 줄 수 있는 wound healing과 cell regeneration에는 적색 및 황색광의 LED array를 20분간 조사하였을 때가 가장 효과적이었으며, 적외광의 경우에는 모든 실험에서 효과가 없음을 확인할 수 있었다.

• Korean Journal of Food Science and Technology
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• v.47 no.6
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• pp.785-788
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• 2015

In this study, the effect of light irradiation on tyrosinase activity during storage in a low temperature incubator was investigated, with the goal of identifying the appropriate wavelength and intensity of light for inhibiting tyrosinase activity. Fluorescent light and blue light emitting diode (LED) irradiation were found to inhibit tyrosinase activity. In particular, tyrosinase activity was significantly inhibited under high intensity blue LED irradiation, suggesting that the activity of tyrosinase present in vegetables or fruits might be inhibited by LED irradiation. Therefore, blue LED irradiation during food storage and the distribution stage would be useful for ensuring good quality of food, owing to the inhibition of the enzymatic browning reaction.

• Journal of Mushroom
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• v.15 no.4
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• pp.259-263
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• 2017

In this study, we investigated the morphological characteristics and antioxidant ability of mushroom cultivar Lentinula edodes 'Nongjingo' fruiting bodies after exposure to various light conditions. Color differences between mushrooms treated with mixtures of LED light revealed that mushrooms displayed lighter color shades when compared to the control group (fluorescent light treated mushrooms). Redness increased and yellowness decreased after exposure to all treatments other than the fluorescent control. Measurement of growth characteristics of 'Nongjingo' fruiting bodies showed increases after exposure to all mixed LED treatments. In addition, the uniformity of fruiting bodies was higher when using LED light compared to fluorescent light. The measurement of stem diameters did not show a significant difference between the treatments, however, diameters were slightly larger with exposure to white-green LED. Moreover, stem length was longer in the mixed LED treatments when compared to those exposed to fluorescent light. Examination of the ratio of stem diameter to stem length revealed that the diameter of the stem was greater than the length. The antioxidant activity of water extracts made from Nongjingo fruiting bodies grown under mixed LED conditions was compared to those from mushrooms grown under fluorescence light conditions. The highest antioxidant activity was observed from mushrooms treated with white LED; however, no significant difference was found between mushrooms exposed to white-green LED compared to white-blue LED. The treatment showed higher antioxidant ability than vitamin C. Our results confirm that treatment of white LED and white-blue LED affects the growth and antioxidant ability of Nongjingo mushroom fruiting bodies.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.1
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• pp.135-140
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• 2014

Pre-storage ultra-violet (UV) light treatment on fresh produce is known to inactivate the contaminated microorganisms, activate the defense system, and delay ripening extending the shelf life. As UV light emitting diode (LED) becomes available at a relatively low price, continuous or intermittent UV treatment during chilled storage is possible in a container or package. This study attempted an in situ UV LED treatment on fresh produce stored under a refrigerated container in order to see its potential in the fresh produce storage and further optimize its application conditions. The effect of in-container UV LED irradiation on the quality preservation of shredded carrots was investigated in the air and modified atmosphere (MA) conditions. Two sets of experiment with Escherichia coli inoculation and with natural microbial flora in the air (two 30 minute on-off cycles of 1 $diode/dm^2$ per day at a location above 2 cm) showed a clear and significant effect of the UV LED irradiation on the suppression of microbial growth: 280 nm was the most effective by maintaining a lower microbial count by at least 0.5 log (CFU/g) throughout the 6 day storage period. The carotenoids content of shredded carrots subjected to UV LED treatment at 365 and 405 nm in the air was higher than that of the control shredded carrots. In MA condition of $O_2$ of 1.2~4.3% and $CO_2$ of 8.4~10.6% being indifferent with LED wavelengths, 280 nm UV LED irradiation was also effective in inhibiting the microbial growth. While there was no observed difference in the carotenoids content between untreated and UV LED-treated shredded carrots in MA, UV LED irradiation at 365 and 405 nm was slightly better in DPPH radical scavenging activity. The use of UV LED in storage container or package seems to give the benefits of preserving the microbial and nutritional qualities of minimally processed fruits and vegetables.