• 대한의생명과학회지
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• 제10권2호
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• pp.65-73
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• 2004

I report that single-stranded antisense as a part of large circular (LC-) genomic DNA of recombinant M13 phage exhibits enhanced stability, sequence specific antisense activity, and no need for target site search. A cDNA fragment (708 bp) of rat TNF-$\alpha$ was inserted into a phagemid vector, and TNF-$\alpha$ antisense molecules (TNF$\alpha$-LCAS) were produced as single-stranded circular DNA. When introduced into a rat monocyte/macrophage cell line, WRT7/P2, TNF$\alpha$-LCAS was able to ablate LPS-induced TNF-$\alpha$ mRNA to completion. The antisense effect of TNF$\alpha$-LCAS was shown to be sequence-specific because expressions of three control genes ($\beta$-actin, GAPDH and IL-1$\beta$) were not significantly altered by the antisense treatment. Further, TNF$\alpha$-LCAS was found to be highly efficacious as only 0.1 $\mu$g (0.24 nM) of TNF$\alpha$-LCAS was sufficient to block TNF-$\alpha$ expression in 1$\times10^5$ WRT7/P2 cells. I have also observed specific antisense activity in reduction of NF-$\kappa$B gene expression. The results suggest that an antisense sequence as a part of single-stranded circular genomic DNA has a specific antisense activity.

• Journal of Microbiology
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• 제44권1호
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• pp.77-82
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• 2006

The tea looper caterpillar, Ectropis obliqua, is one of the major pests of tea bushes. E. obliqua single-nucleocapsid nucleopolyhedrovirus (EcobSNPV) has been used as a commercial pesticide for biocontrol of this insect. However only limited genetic analysis for this important virus has been done up to now. EcobSNPV was characterized in this study. Electron microscopy analysis of the occlusion body showed polyhedra of 0.7 to $1.7\;{\mu}m$ in diameter containing a single nucleocapsid per envelope of the virion. A 15.5 kb genomic fragment containing EcoRI-L, EcoRI-N and HindIII-F fragments, was sequenced. Analysis of the sequence revealed that the fragment contained eleven potential open reading frames (ORFs): lef-1, egt, 38.7k, rrl, polyhedrin, orfl629, pk-1, hoar and homologues to Spodoptera exigua multicapsid NPV (SeMNPV) ORFs 15, 28, and 29. Gene arrangement and phylogeny analysis suggest that EcobSNPV is closely related to the previously described Group II NPV. Bioassays on lethal concentration $(LC_{50}\;and\;LC_{90})$ and lethal time $(LT_{50}\;and\;LT({90})$ were conducted to test the susceptibility of E. obliqua larvae to the virus.

• 한국전기전자재료학회논문지
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• 제18권8호
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• pp.724-728
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• 2005

Electro-optic characteristics of reflective hybrid aligned liquid crystal (LC) cell driven by fringe field using a nematic LC with positive dielectric anisotropy have been studied. Optimized optical configurations are achieved by using a single polarizer, half-wave film and a cell with quarter-wave retardation. The simulation results shows an optimum cell retardation of $0.30{\mu}m$. This value may allow a practical cell gap larger than $3{\mu}m$, which makes it easy to control in the manufacturing process. Furthermore, this LC cell with optimized cell parameters shows low wavelength dispersion and the contrast ratio greater than 5 over exists about $100{\circ}$ in vertical direction and $160{\circ}$ in horizontal direction. Also, when using the LC with positive dielectric anisotropy rather than negative dielectric anisotropy, the display shows low power consumption and fast response time.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1996년도 하계학술대회 논문집 A
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• pp.553-555
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• 1996

This paper concerns with a method to improve the current and power waveforms of the variable speed $3{\Phi}$ AC motor system driven by the single-pulse PWM, and the current waveforms and $3{\Phi}$ power waveforms are analyzed by FFT under various running conditions. By the performance analysis through the experiments, the following results have been obtained. AC source of pseudo sine waveform can be obtained from the modulated alternating square voltage by means of the well designed LC filter. It is confirmed that current waveforms and $3{\Phi}$ power waveforms can be improved by utilizing the high order low pass LC filter than that of lower order. Especially, current waveforms and $3{\Phi}$ power waveforms tan be much improved at low frequency domain. $3{\Phi}$ power waveforms have a smatter ripple and bigger power by utilizing the high order low pass LC filter than that of lower order. Also, the running condition of $3{\Phi}$ AC motor is good at low frequency domain.

• 전기학회논문지
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• 제65권4호
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• pp.601-610
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• 2016

The light-emitting diode (LED) has been used in a variety of industrial fields and for general 0lighting purposes on account of its high efficiency, low power consumption and long lifespan. The LED is driven by direct current; therefore, an AC/DC converter is typically required for its use. An electrolytic capacitor is generally used for stabilizing DC voltage during use of the AC/DC converter. However, this capacitor has a short lifespan, which makes it a limiting factor in LED lighting. Furthermore, LED lighting requires a dimmable control to enable energy savings and fulfil a growing consumer demand. In this paper, the dimmable single-stage power factor correction (PFC) continuous conduction mode (CCM) flyback converter that employs no electrolytic capacitor is presented. The LC resonant filter is alternatively applied to reduce the 120[Hz] ripple on the output. And the optimum value of the LC resonant filter parameters considering both efficient and performance is analysed. Simulation and experimental results verify the satisfactory operation of the converter.

• 한국해양공학회지
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• 제15권2호
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• pp.99-104
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• 2001

Elastic-plastic fracture toughness $J_{lc}$ can be used as an effective design criterion in elastic plastic fracture mechanics. Most of these systems are$J_{lc}$ $J_{lc}$ value at high temperature must be determined for use of integrity evaluation and designing of such systems. Elastic-plastic fracture toughness $J_{lc}$ tests were performed on SA516/70 carbon steel plate and test results were analyzed according to ASTM E 813-87, ASTM E 813-89 and ASTM E 1152-87.safety and integrity are required for reactor pressure vessels because, they are operated in high temperature. There are single specimen method, which used as evaluation of safety and integrity for reactor pressure vessels. In this study, elastic-plastic fracture toughness($J_{lc}$) and J-$\Delta$a of SA 516/70 steel used as reactor pressure vessel steel are measured and evaluated at room temperature, 150$^{\circ}C $, 250$^{\circ}C $ and 370$^{\circ}C $ according to unloading compliance method.

• Journal of Power Electronics
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• 제9권6호
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• pp.929-939
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• 2009

This paper presents a new soft-switching boost converter based on the LC resonance and passive clamping technique without additional active switches. The circuit achieves high efficiency and low voltage stress by adopting a soft switching method using LC resonance. This paper gives a mathematical analysis of each mode and a detailed design procedure of the proposed boost converter. First of all, the operational principles are verified through simulation results. Then, according to the design procedure, we designed and built a 1.5[kW] prototype soft switching boost converter. Through the experimental results, we demonstrated the validity and usefulness of the proposed boost converter.

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2007년도 7th International Meeting on Information Display 제7권2호
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• pp.1125-1128
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• 2007

The laminating technique for developing flexible liquid crystal display was demonstrated by using a thin UV curable polymer film and a plastic substrate with patterned polymer wall structure. We adopted the rigid wall structure to provide a solid mechanical support for the stable molecular alignment of liquid crystals (LCs) in the device. The cover film was prepared to have an ability of aligning LC molecules by patterning a micro-groove structure using the soft-lithographic process. These two substrates can be assembled tightly by the laminating and one-step UV irradiation process because of the adhesive nature of the used UV curable polymers. Proposed method can be used to fabricate the flexible LC display with simplicity and also be applicable for a cost-effective roll-to-roll process.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2004년도 춘계학술대회 논문집
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• pp.1334-1339
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• 2004

This paper proposes transformerless power conversion system consisting of a single-phase diode rectifier and a shunt hybrid filter for the electric-railway system. The hybrid filter consists of a single tuned LC filter per a phase and a low-rated NPC type multi-level inverter. Compared with conventional active filters. Transformers are not used. Also, LC filter works as not only a harmonic filter tuned at the 5th harmonic frequency but also a switching-ripple filter. The rating of the active filter can be decreased by using a NPC type multi-level inverter. The simulation results confirm the validity of the system.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2004년도 춘계학술대회 논문집
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• pp.1427-1432
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• 2004

This paper proposes transformerless power conversion system consisting of a single-phase diode rectifier and a shunt hybrid filter for the electric-railway system. The hybrid filter consists of a single tuned LC filter per a phase and a low-rated NPC type multi-level inverter. Compared with conventional active filters. Transformers are not used. Also, LC filter works as not only a harmonic filter tuned at the 5th harmonic frequency but also a switching-ripple filter. The rating of the active filter can be decreased by using a NPC type multi-level inverter. The simulation results confirm the validity of the system