• 디자인학연구
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• 제19권2호
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• pp.323-332
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• 2006

휴대폰, 스마트폰, PDA 등 모바일 정보기기의 크기는 점차 줄어들어 이제는 거의 부담 없이 호주머니에 넣고 사용할 수 있게 되었다. 하지만 이와 같은 정보기기의 극적인 소형화는 텍스트 기반 컨텐츠에 대한 가독성을 저하시키는 원인이 되고 있다. 모바일 정보기기의 소지용이성과 텍스트 가독성은 크기와 비례 등 형태적 속성과 밀접한 관련을 갖는다. 따라서 본 연구는 두 가지 사용성 요소가 우수하게 양립할 수 있는 정보기기의 형태적 특성을 탐색하는데 목적을 두었다. 이를 위해 우선 정보기기 모형의 크기$(4000mm^2/8000mm^2)$ 비례(1:1/2:1/13:1), 무게(100g/200g) 등 물리적 요인과 소지방식(바지 주머니/손)과 소지자세(선 자세/암은 자세) 등에 따라 소지용이성이 어떻게 변화하는지를 실험하였다. 그 결과 남성의 경우 바지 속에 모형을 넣고 소지용이성을 평가한 결과 2:1비례를 선호하는 경향이 있었다. 손에 쥐고 소지용이성을 평가한 여성의 경우는 모형의 면적 $400mm^2 (두째:20mm)인 모형에서는 2:1비례를, $8000mm^2$ (두께:20mm)에서는 3:1 비례를 선호하였다. 전반적으로 무게에 의한 소지용 이성의 변화가 $4000mm^2$ 면적의 모형에서 현저히 나타났다. 따라서 전체적인 소지용이성을 향상하기 위해서는 2:1비례가 적정한 것으로 나타났다. 두 번째로 모바일 정보기기의 크기$(2000mm^2/4000mm^2/8000mm^2)$와 비례(3:1/2:1/1:1/2:1/3:1) 등 형태적 속성과 행간(135%/200%)이 텍스트 가독성에 어떠한 영향을 미치는지 실험하였다. 그 결과 대부분의 경우 행폭의 증가가 독서속도를 향상시키고 있음을 알 수 있었다. 또한 독서에 대한 주관적 만족도가 2:1비례에서 가장 우수함을 알 수 있었다. 이상의 실험결과를 바탕으로 본 연구는 모바일 정보기기의 소지용이성과 텍스트 가독성을 우수하게 양립시킬 수 있는 2:1의 형태 비례를 발견할 수 있었다. 실질적으로 연구결과를 활용하기 위해서는 모바일 정보기기에서 표시창외에 입력장치를 위한 공간을 추가로 확보해야 한다는 사실을 감안하여 디자인 작업에 적용해야 할 것이다.

• 한국농공학회지
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• 제22권1호
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• pp.53-66
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• 1980

The research work is concerned with the analytical and experimental studies on the heat transfer phenomenon around the underground concrete digester used for biogas production Systems. A mathematical and computational method was developed to estimate heat losses from underground cylindrical concrete digester used for biogas production systems. To test its feasibility and to evaluate thermal parameters of materials related, the method was applied to six physical model digesters. The cylindrical concrete digester was taken as a physical model, to which the model,atical model of heat balance can be applied. The mathematical model was transformed by means of finite element method and used to analyze temperature distribution with respect to several boundary conditions and design parameters. The design parameters of experimental digesters were selected as; three different sizes 40cm by 80cm, 80cm by 160cm and l00cm by 200cm in diameter and height; two different levels of insulation materials-plain concrete and vermiculite mixing in concrete; and two different types of installation-underground and half-exposed. In order to carry out a particular aim of this study, the liquid within the digester was substituted by water, and its temperature was controlled in five levels-35。 C, 30。 C, 25。 C, 20。C and 15。C; and the ambient air temperature and ground temperature were checked out of the system under natural winter climate conditions. The following results were drawn from the study. 1.The analytical method, by which the estimated values of temperature distribution around a cylindrical digester were obtained, was able to be generally accepted from the comparison of the estimated values with the measured. However, the difference between the estimated and measured temperature had a trend to be considerably increased when the ambient temperature was relatively low. This was mainly related variations of input parameters including the thermal conductivity of soil, applied to the numerical analysis. Consequently, the improvement of these input data for the simulated operation of the numerical analysis is expected as an approach to obtain better refined estimation. 2.The difference between estimated and measured heat losses was shown to have the similar trend to that of temperature distribution discussed above. 3.It was found that a map of isothermal lines drawn from the estimated temperature distribution was very useful for a general observation of the direction and rate of heat transfer within the boundary. From this analysis, it was interpreted that most of heat losses is passed through the triangular section bounded within 45 degrees toward the wall at the bottom edge of the digesten Therefore, any effective insulation should be considered within this region. 4.It was verified by experiment that heat loss per unit volume of liquid was reduced as the size of the digester became larger For instance, at the liquid temperature of 35˚ C, the heat loss per unit volume from the 0. 1m$^3$ digester was 1, 050 Kcal/hr m$^3$, while at for 1. 57m$^3$ digester was 150 Kcal/hr m$^3$. 5.In the light of insulation, the vermiculite concrete was consistently shown to be superior to the plain concrete. At the liquid temperature ranging from 15。 C to 350 C, the reduction of heat loss was ranged from 5% to 25% for the half-exposed digester, while from 10% to 28% for the fully underground digester. 6.In the comparison of heat loss between the half-exposed and underground digesters, the heat loss from the former was fr6m 1,6 to 2, 6 times as much as that from the latter. This leads to the evidence that the underground digester takes advantage of heat conservation during winter.

• 한국수정란이식학회지
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• 제20권2호
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• pp.169-176
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• 2005

수정란의 성 판별은 유전적으로 우수한 유전형질을 보유하고 있는 소의 수정란을 성 판별하므로서 희망하는 성의 송아지를 생산할 수 있으며, 부가가치가 높은 수정란을 확보할 수 있는 기술이다. 수정란의 손상을 최소화하면서 할구를 biopsy하는 기술을 개발하고, 간단하고 빠른 시간에 성 판별이 가능한 Loop-mediated isothermal amplification방법으로 수정란을 성 판별을 실시한 결과는 다음과 같다. 1. 한우 체내 수정란의 성비는 암컷이 $56.5\%$, 수컷이 $43.5\%$였고, 체외 수정란은 암컷이 $49.2\%$, 수컷이 $33.9\%$였다. 그리고, 젖소 체외 수정란의 성비는 암컷이 $29.2\%$, 수컷이 $70.8\%$를 나타내어 한우 체내 및 체외 수정란보다 젖소 체외수정란의 수컷비율이 유의적으로 높게 나타내었다(p<0.05). 또한, 한우 체외 수정란에서 성 판별이 불가능한 것이 $16.9\%$를 나타내어 한우 체내 수정란 및 젖소 체외 수정란과는 유의적인 차이를 나타내었다.(P<0.05). 2. Biopsy한 체내 수정란의 체외 발달율은 $100\%$였으나 체외 수정란에서는 정상적으로 발달하지 못하고 퇴화된 수정란이 $13.2\%$로 체내 수정란보다 유의적으로 높은 결과를 나타내었다.(P<0.05). 3. Punching 방법으로 수정란의 biopsy 후 정상적으로 발달하지 못하고 퇴화된 수정란은 체내 및 체외 수정란에서는 없었으나 biopsy 방법으로 biopsy한 수정란은 체내 및 체외 수정란에서 각각 $16.7\%$ 및 $22.6\%$를 나타내어 Punching 방법보다 유의적으로 높은 결과를 나타내었다(P<0.05). 이상의 결과로 보아 한우 체내 수정란은 LAMP방법을 이용하여 간단하고 신속하게 성 판별이 가능하며, 수정란의 biopsy는 punching 방법이 수정란에 손상을 적게 주는 것으로 사료된다.

• 한국식품위생안전성학회지
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• 제37권3호
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• pp.136-142
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• 2022

메토프렌은 살충제로 곤충의 성장을 방해하는 유충호르몬 유사체로 널리 사용되고 있다. 국내 축산물에 대한 MRL은 0.05-0.1 mg/kg 수준으로 설정되어 있지만 PLS제도가 축산물을 대상으로 확대될 수 있기 때문에 0.01 mg/kg 이하에서 정량이 가능한 시험법이 필요하다. 기존의 식품공전에 존재하던 시험법(식품의약품안전처 공고 제 2021-69호, '21.8.9.)은 우유와 그 외 축산물의 전처리 과정이 서로 상이할 뿐 아니라 충전칼럼을 사용하여 노후화되고 복잡하다는 단점이 있다. 본 연구에서는 비교적 간편하며 분석 시간이 적게 소요되는 QuEChERS법을 활용하여 0.01 mg/kg의 정량한계를 만족하는 메토프렌 시험법을 마련하고자 하였다. 메토프렌의 물리·화학적 특성을 고려하여 1% 아세트산을 함유한 아세토니트릴:아세톤(1:1) 혼합액을 이용하여 진탕 추출 후 d-SPE를 이용한 정제조건을 확립하여 LC-MS/MS를 이용한 시험법을 개발하였다. 메토프렌의 결정계수(R²)는 0.99 이상으로 높은 직선성을 보여주었고, 정량한계는 0.01 mg/kg으로 높은 감도를 나타내었다. 대표 축산물 6종(소고기, 돼지고기, 닭고기, 우유, 계란, 지방)에 대하여 정량한계, 정량한계 10배, 정량한계 50배 수준으로 처리한 다음 회수율 실험을 한 결과 평균 회수율이 79.5-105.1%이었으며 상대표준편차는 14.2% 이하로 확인되었다. 본 연구는 국제식품규격위원회 가이드라인(Codex Alimentarius Commission, CAC/GL40)의 잔류농약 분석 기준 및 식품의약품안전평가원의 '식품등 시험법 마련 표준절차에 관한 가이드라인(2016)'에 적합한 수준임을 확인하였다. 따라서 본 연구에서 개발한 시험법은 추후 교차검증을 거쳐 축산물 중 잔류할 수 있는 메토프렌의 안전관리를 위한 공정시험법으로 활용 가능할 것이다.

• 농약과학회지
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• 제19권3호
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• pp.329-334
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• 2015

병든 토마토, 딸기, 오이 등의 열매에서 분리한 86개의 Botrytis cinerea의 균주를 가지고 한천희석법을 통하여 mepanipyrim의 균사생장 억제효과를 조사하였으며, $EC_{50}$값을 계산하여 저항성 모니터링을 실시하였다. 병원균의 저항성 모니터링을 PDA 배지에서 실시할 경우 mepanipyrim의 균사생장 억제효과를 정확하게 검정할 수 없었으나, FGA 배지를 사용하였을 때에는 감수성과 저항성 반응을 뚜렷하게 구분할 수 있었다. 실험에 사용한 B. cinerea의 86개 균주를 $1.0{\mu}g\;mL^{-1}$의 $EC_{50}$값을 가지고 감수성과 저항성을 구분하였으며, 전체 균주 중에서 20개인 23.3%가 저항성균으로 분류되었다. 지역에 따라서 저항성균의 분리비에는 차이가 있었는데, 경기, 경남/부산, 그리고 충남에서 각각 분리한 균주 중에서 저항성균의 비율은 28.6, 33.3, 12.8%로, 경남/부산 지역에서 가장 높았다. Mepanipyrim에 대한 저항성균은 pyrimethanil에 대해서도 교차저항성을 보였으며, 오이 자엽에 대한 병원성을 감수성 균주와 비교해 보아도 전혀 떨어지지 않았다. 따라서 포장에서 anilinopyrimidine계 살균제를 효과적으로 사용하기 위해서 적극적인 관리가 필요한 상태이다.

• 대한간호학회지
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• 제6권2호
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• pp.1-9
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• 1976

Presently, there is increasing demand for geriatric nursing care because of increasing preparation of elderly population due to extended age. Of particular importance in determining the effectiveness of nursing care given to the elderly is the attitude of the nurse toward the elderly patient. Knowledge of the various changes that usually occur with aging will enable the nurse to help an aged Person maximize his potential in illness and in health. The objectives of this study were : 1. To delineate the attitudes of nurses toward the geriatric nursing care. 2. To learn the influencing factors affecting the attitudes of nurses toward the geriatric nursing care. The study population defined and randomly selected is 225 nurses at one general hospital, one private hospital, two national hospitals in Seoul during the period of October l0th -20th, 1975. The questionnaire method was used. Respondents were 140 nurses. X$^2$- test and t- test were employed in analyzing the data. The questionnaire form included 54 statements which concerned the attitudes of nurses to-ward geriatric nursing care. It was divided into five areas : 1) General characteristics of study population. 2) The nurse's concepts of elderly. 3) Care of the geriatric patient. 4) Interpersonal relationship with geriatric patient. 5) Teaching of the geriatric patient. Each of the 54 statements of the questionnaire was considered to be either Positive or Negative. A Positive response was assigned the value of＋1 , and a Negative response or no response was assigned the value of O. The results of the study were as follows : 1. The Investigation of attitudes of nurses toward geriatric nursing care. a. Data indicated , respondents have negative attitudes in their. concepts of elderly (74.3%) b. Data indicated respondents have positive attitudes in care of geriatric patient (64.3%). c. Data indicated respondents have positive attitudes in interpersonal relationship with geriatric patient (85% ). 4. Data indicated respondents have positive attitudes in teaching of geriatric patient (89.3%). 2. The results of study regarding the five hypothesis were as follows : a There was significant difference in the mean scores between the attitudes of nurses toward the geriatric nursing care and level of education of the nurse. b. There was no significant difference between the attitudes of nurses toward the geriatric nursing care and clinical experience, but there was significant difference between care of the geriatric patient and clinical experience. c. There was no significant difference between the attitudes of nurses toward the geriatric nursing care and shift most frequently worked, but there was significant difference between care of the geriatric patient and shift most frequently worked. d. There was no significant difference in the mean scores between the attitudes of nurses toward the geriatric nursing care and marital status, but there was significant difference in the mean scores between interpersonal relationship, teaching of geriatric patient and marital status. e, There was no significant difference in the mean scores between the attitudes of nurses toward the geriatric nursing care and experience with elderly, but there was significant difference in the mean scores between care of geriatric patient and experience with elderly.

• Journal of Korean Neurosurgical Society
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• 제63권1호
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• pp.45-55
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• 2020

Objective : Fibrin sealants have been used for hemostasis, sealant for cerebrospinal fluid leakage, and adhesive barrier in neurosurgery. Further, as its clinical use and role of an effective drug delivery vehicle have been proposed. This study was performed to measure antibacterial activity and continuous local antibiotic release from different concentrations of vancomycin-impregnated fibrin sealant in vitro. Methods : Antibacterial activity was investigated by disk diffusion test by measuring the diameter of the growth inhibition zone of bacteria (methicillin-resistant Staphylococcus aureus, ATCC29213) from vancomycin-embedded fibrin sealant disc diluted at five different concentrations (C1-C5; 8.33, 4.167, 0.83, 0.083, and 0.0083 mg/disc, respectively). Continuous and conditioned release of vancomycin concentration (for 2 weeks and for 5 days, respectively) were also measured using high-performance liquid chromatography (HPLC) method. To mimic the physiologic wound conditions with in vitro, conditioned vancomycin release in phosphate buffer solution (PBS) was measured and replaced PBS for five consecutive days, half a day or completely daily. Results : In the disk diffusion test, the mean diameters of bacterial inhibition zone were 2.54±0.07 cm, 2.61±0.12 cm, and 2.13±0.15 cm (C1, C2, and C3 respectively) but 1.67±0.06 cm and 1.23±0.15 cm in C4 and C5, respectively. Continuous elution test elicited the peak release of vancomycin from the fibrin sealant at 48 hours, with continued release until 2 weeks. However, conditioned vancomycin release decreased to half or more on day 2, however, the sustainable release was measured over the therapeutic dose (10-20 ㎍/mL) for 5 days and 4 days in assays of half and total exchange of PBS. Conclusion : This study suggests that fibrin sealant can provide an efficient vehicle for antibiotic drug release in a wide range of neurosurgical procedures and the safe and effective therapeutic dose will be at the concentration embedded of 4.167 mg/disc or more of vancomycin.

• The Plant Pathology Journal
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• 제25권3호
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• pp.280-285
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• 2009

Powdery and downy mildews caused by Sphaerotheca fusca and Pseudoperonospora cubensis are the most common and serious diseases of cucumber worldwide. In spite of the introduction of highly effective systemic fungicides, control of these diseases remains elusive. Hence, this study aimed to develop an alternative method to chemicals in controlling the diseases by using different types of cooking oil. Egg yolk, which contains a natural emulsifier, lecithin, was selected as a surfactant to emulsify the oils. Among the different cooking oils used, soybean, canola (rape seed), safflower, sunflower, olive, and corn oils showed over 95% control values against powdery mildew of cucumber in a greenhouse test. In particular, 0.3% canola oil emulsified with 0.08% yolk (1 yolk and 60 ml canola in 20 l spray) was found to be the most effective. The treatment resulted in 98.9% and 96.3% control efficacies on powdery and downy mildews, respectively, of cucumber in the field. Canola oil exhibited direct and systemic effect, wherein powdery mildew of cucumber was suppressed only on treated leaves but not on non-treated leaves in a plant, while mycelia and conidia of the pathogen were severely distorted or destroyed by the treatment. The prospect of using the canola oil and yolk mixture as a natural fungicide is highly promising because of its effectiveness, availability, low cost, simple preparation, and safety to humans and the environment. The use of the canola oil and yolk mixture is expected to be an effective fungicide for use in organic farming and home gardening.

• Clinical and Experimental Reproductive Medicine
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• 제31권1호
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• pp.67-74
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• 2004

Objective: This study was to examine in vitro neural cell differentiation pattern of the genetically modified human embryonic stem cells expressing tyrosine hydroxylase (TH). Materials and Methods: Human embryonic stem (hES, MB03) cell was transfected with cDNAs cording for TH. Successful transfection was confirmed by western immunoblotting. Newly transfected cell line (TH#2/MB03) was induced to differentiate by two neurogenic factors retinoic acid (RA) and b-FGF. Exp. I) Upon differentiation using RA, embryoid bodies (EB, for 4 days) derived from TH#2/MB03 cells were exposed to RA ($10^{-6}M$)/AA ($5{\times}10^{-2}mM$) for 4 days, and were allowed to differentiate in N2 medium for 7, 14 or 21 days. Exp. II) When b-FGF was used, neuronal precursor cells were expanded at the presence of b-FGF (10 ng/ml) for 6 days followed by a final differentiation in N2 medium for 7, 14 or 21 days. Neuron differentiation was examined by indirect immunocytochemistry using neuron markers (NF160 & NF200). Results: After 7 days in N2 medium, approximately 80% and 20% of the RA or b-FGF induced Th#2/MB03 cells were immunoreactive to anti-NF160 and anti-NF200 antibodies, respectively. As differentiation continued, NF200 in RA treated cells significantly increased to 73.0% on 14 days compared to that in b-FGF treated cells (53.0%, p<0.05), while the proportion of cells expressing NF160 was similarly decreased between two groups. However, throughout the differentiation, expression of TH was maintained ($\sim$90%). HPLC analyses indicated the increased levels of L-DOPA in RA treated genetically modified hES cells with longer differentiation time. Conclusion: These results suggested that a genetically modified hES cells (TH#2/MB03) could be efficiently differentiated in vitro into mature neurons by RA induction method.

• 대한지리학회지
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• 제37권4호
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• pp.371-384
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• 2002

지역개발의 결과인 지표환경의 변화를 예측하는 일은 지속 가능한 환경을 보전하기 위한 수단으로서 대단히 중요하다. 본 연구에서는 다중분광영상 자료를 이용한 퍼지논리연산을 통하여 안면도의 최근 20년(1901~2000) 간의 지표경관 변화를 예측하고 실제 변화된 내용과 비교 검토하였다. 안면도에 대한 문헌, 지도 및 현지 답사한 결과에 의하면, 1980년대 이전에 주로 해안간척과 황무지개간, 초지 조성 등으로 토지이용 형태가 변화되어 왔다. 그러나 1980년대 중반 이후부터는 해수욕장 주변과 준농림지역을 중심으로 민박시설과 점포 등이 무질서하게 들어섰으며, l990년대에는 관광지 개발 및 토시지역의 확장으로 농경지는 증가하였으나 해안지역과 취락지역의 산림이 심각하게 훼손되었다. 퍼지논리연산을 이용하여 지표환경 변화를 통합하여 예측한 결과와 2000년 위성영상에서 얻은 안면도의 지표환경은 비교적 정확하게 일치하였다. 안면도 지역에서 대규모 토지피복 변화가 일어날 가능성이 높은 지역들은 해안과 가까운 평지에 위치한 지역으로 예측되었는데 실제로 이 지역은 현재 대규모 개발이 진행 중이어서 연안환경 악화의 우려를 자아내고 있다. 따라서 본 방법은 향후 지표환경 변화 연구를 위한 효과적으로 적용될 수 있을 것으로 기대된다.