• Title/Summary/Keyword: L1

Search Result 54,027, Processing Time 0.067 seconds

Secretion of Cytokine Stimulating Intercellular Adhesion Molecule-l Expression from THP-l Cells Infected with Human Cytomegalovirus (HCMV에 감염된 TBP-1 세포에서 세포간 부착분자-1 발현 촉진물질의 방출)

  • Kim, Mi-Suk;Yi, Hyun-Ah;Lee, Chan-Hee
    • Korean Journal of Microbiology
    • /
    • v.44 no.2
    • /
    • pp.140-146
    • /
    • 2008
  • Human cytomegalovirus (HCMV) stimulates the expression of intercellular adhesion molecule (ICAM-l) on the surface of monocytic THP-1 cells. Stimulation of ICAM-l did not require HCMV gene expression since UV-inactivated HCMV (UV-HCMV) was able to induce ICAM-l expression. ICAM-l expression was also stimulated in uninfected THP-l cells which were fed with culture supernatant of HCMV-infected THP-l cells. Co-culture experiment using trans-well insert supported that HCMV-infected THP-l cells secreted some cytokine(s) stimulating ICAM-l expression. The stimulation of ICAM-l by HCMV-infected cell culture supernatant appears to involve $NF-{\kappa}B$ pathway. Culture supernatant from THP-l cells infected with UV-HCMV, whose gene expression was abrogated, failed to stimulate ICAM-l expression on naive THP-l cells. Thus, HCMV gene expression seems to be required in secretion of cytokine(s) stimulating ICAM-l expression.

Mixed Convection Heat Transfer from Two Vertical Parallel Plates with Different Conditions (조건이 다른 수직 평형 평판에서 혼합대류 열전달)

  • Kim, S.Y.;Chung, H.S.;Kwon, S.S.
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
    • /
    • v.4 no.4
    • /
    • pp.243-252
    • /
    • 1992
  • A mixed convection heat transfer from two vertical parallel plates has been studied numerically by the finite difference method. Effects of the Grashof number, the relative length, $L_2/L_1$. the dimensionless temperature ratio, ${\Phi}_2/{\Phi}_1$ and the dimensionless plate spacing, $b/L_1$ are examined for the heat transfer. Independent of the Grashof numbers and $L_2/L_1$, the dimensionless vertical velocity distributions skewed on the left plate as ${\Phi}_2/{\Phi}_1$ decreased. The dimensionless vertical velocity distribution for $Gr/Re^2=1$ and ${\Phi}_2/{\Phi}_1=1.0$ is skewed to the right plate $L_2/L_1=0.5$, symmetric at $L_2/L_1=1.0$ and skewed to the left plate at $L_2/L_1=1.5$. But for $Gr/Re_2=10.0$ and ${\Phi}_2/{\Phi}_1=1.0$ reversed velocity patterns are obtained. Regardless of the Grashof numbers and $L_2/L_1$, the mean Nusselt nembers on the inside surface of the left plate decreases and those of the right inside surface increases as ${\Phi}_2/{\Phi}_1$ increases. Temperature, velocity and mean Nusselt number distributions are apparently not affected by $L_2/L_1$.

  • PDF

Effects of Duroc Breeding Lines on Carcass Composition and Meat Quality

  • Choi, Jung-Seok;Jin, Sang-Keun;Choi, Yang-Il;Lee, Jae-Joon
    • Food Science of Animal Resources
    • /
    • v.35 no.1
    • /
    • pp.80-85
    • /
    • 2015
  • This study was performed to investigate the carcass composition and pork quality of Duroc breeding lines in Korea. A total of 200 Duroc pigs were used, and those were originated from four different great-grandparent (GGP) breeding stock farms (L1: N farm, L2: W farm, L3: S farm, L4: R farm). The carcasses of pigs from these farms were collected, and meat quality traits were evaluated. L1 and L2 had smaller carcass weights and thin backfat, whereas L3 and L4 had heavy carcass weights and thick backfat. L3 and L4 had higher contents of fat and protein than L1 and L2. For the meat quality characteristics, L1, L2, and L4 had higher pH values than L3. In addition, L4 had higher water holding capacity than the other lines. L4 had the highest sensory evaluation scores with regard to both juiciness and flavor. Consequently, the study results indicate that pork quality information from domestic Duroc breeding stock lines could be used to effectively improve pork quality in Korea.

COMPLEMENTED SUBLATTICES OF $\omega L_i$ ISONMORPHIC TO CLASSICAL BANACH LATTICES

  • Kang, Jeong-Heung
    • Communications of the Korean Mathematical Society
    • /
    • v.11 no.4
    • /
    • pp.1015-1030
    • /
    • 1996
  • We investigate complemented Banach subspaces of the Banach envelope of $eak L_1$. In particular, the Banach envelope of $weak L_1$ contains complemented Banach sublattices that are isometrically isomorphic to $l_p, (1 \leq p < \infty)$ or $c_0$. Finally, we also prove that the Banach envelope of $weak L_1$ contains an isomorphic copy of $l^{p, \infty}, (1 < p < \infty)$.

  • PDF

Callus Induction and Somatic Embryogenesis from Sicyos angulatus L. (야생식물 Sicyos angulatus L.로부터 캘러스 유도 및 체세포배 발생)

  • 권순태;조문수
    • Korean Journal of Plant Tissue Culture
    • /
    • v.25 no.2
    • /
    • pp.119-123
    • /
    • 1998
  • In order to investigate the possibility of in vitro mass propagation via somatic embryogenesis from Sicyos angulatus L., effects of plant growth regulators and carbon sources on callus induction and somatic embryogenesis were evaluated. Optimal combinations of plant growth regulator for callus induction from cotyledon and inflorescence explants were 2,4-D 2.0 mg/L + BA 0.1 mg/L and 2,4-D 1.0 mg/L + BA 0.1 mg/L in MS basal medium supplemented with sucrose 30 g/L,, respectively. Somatic embryogenesis was observed from cultured inflorescence explants, but it could not be achieved from leaf or cotyledon explants. The most effective plant growth regulators for somatic embryogenesis from callus was NAA 1.0 mg/L + kinetin 10 mg/L in the half strength of MS basal medium supplemented with 20 g/L sucrose.

  • PDF

Plant Regeneration Through Adventitious Bud Formation and Callus Induction from Scales of Lilium lancifolium Thunb. (참나리 (Lilium lancifolium Thunb.) 인편으로부터 부정아 발생과 캘러스 유도를 통한 식물체 재생)

  • Nam, Sang-Wook;Kim, Hei-Young
    • Journal of Plant Biotechnology
    • /
    • v.30 no.1
    • /
    • pp.53-58
    • /
    • 2003
  • This study was performed to investigate the effects of various media compositions in regeneration of Lilium lancifolium. The adventitious bud initiation from microscale was the best on MS medium supplemented with BAP 1.0 mg/L and NAA 0.1 mg/L after 4 weeks of culture. However, from bulbscales, adventitious bud initiation was the best in dark condition on MS medium supplemented with BAP 0.5 mg/L and NAA 0.1 mg/L. On the other hand, callus induction was found to be the best from the microscales incubated in complete dark condition for 8 weeks on MS medium supplemented with 2,4-D 1.0 mg/L and BAP 0.1 mg/L. The highest plantlet regeneration from callus was obtained after incubation in the light condition for 8 weeks on MS medium supplemented with NAA 0.5 mg/L and BAP 0.1 mg/L. Rooting of shoots was obtained easily on MS medium and the plantlets were transferred to soil pots after 8 weeks. The chromosome analysis of the root tip cells was revealed that the callus-derived plantlets had normal chromosome number, 2n=24. No variation was observed in the morphology of the plantlets.

Effect on the Concentration of Glucose and Sucrose on the Hydrogen Production using by the Facultative Anaerobic Hydrogen Producing Bacterium Rhodopseudomonas sp. MeL 6-2 (통성혐기성 수소생산균주 Rhodopseudomonas sp. MeL 6-2를 이용한 수소생산효율에 미치는 포도당 및 자당 농도의 영향)

  • Lee, Eun-Young
    • Microbiology and Biotechnology Letters
    • /
    • v.37 no.2
    • /
    • pp.176-182
    • /
    • 2009
  • Hydrogen producing bacterium, strain MeL 6-2 was isolated from the sludge of the factory areas in Anyang through the acclimation in basal salt medium (BSM) supplemented with 10 g/L of sucrose. Isolated strain MeL 6-2 was a facultative anaerobe which could grow in both aerobic and anaerobic environments. An aerobically grown pure culture isolated from enriched culture was analyzed by 16S rDNA sequencing and identified as Rhodopseudomonas sp. MeL 6-2. Effects of the concentrations of glucose and sucrose on the hydrogen production rate and the hydrogen production yield were investigated. When glucose in the range of 1~12 g/L was supplemented to the BSM, strain MeL 6-2 could grow without lag phase. An increased glucose concentration increased the specific hydrogen production rate linearly to $4.2\;mmol-H_2{\cdot}L^{-1}{\cdot}h^{-1}$ at 10 g/L, and $60\;mmol-H_2{\cdot}mg-DCW^{-1}{\cdot}h^{-1}$, but decreased slightly as the concentration increased to 12 g/L. The hydrogen production yield was maintained over a range from 2.6 to $3.1\;mol-H_2{\cdot}mol-glucose^{-1}$. When sucrose in the range of 1~12 g/L was supplemented to the BSM, strain MeL 6-2 could grow after ten hours. An increased sucrose concentration increased the specific hydrogen production rate and the hydrogen production yield to $163\;mmol-H_2{\cdot}mg-DCW^{-1}{\cdot}h^{-1}$ and to $4.5\;mol-H_2{\cdot}mol-sucrose^{-1}$, respectively.

Rapid Multiplication of M.9 Apple Rootstocks in vitro (M.9 계통 사과 대목의 기내 급속 번식)

  • Jun, Ji Hae;Chung, Kyeong Ho;Jeong, Sang Bouk;Hong, Kyung Hy;Kang, Sang Jo
    • Horticultural Science & Technology
    • /
    • v.19 no.1
    • /
    • pp.34-38
    • /
    • 2001
  • This experiment was conducted to find out the optimum cultural conditions for the propagation of M.9 apple rootstocks such as EMLA M.9 and NAKB T-337 in vitro. Murashige and Skoog (MS) medium supplemented with $1.0mg{\cdot}L^{-1}$ BA, $0.1mg{\cdot}L^{-1}$ IAA, $30g{\cdot}L^{-1}$ sucrose, and $8g{\cdot}L^{-1}$ agar was suitable for shoot proliferation. Removing of apical meristem and horizontal placing of explants on medium increased shoot proliferation significantly. The best rooting was obtained on 1/2 MS medium supplemented with 0$0.5mg{\cdot}L^{-1}$ IBA, $20g{\cdot}L^{-1}$ sucrose, and $8g{\cdot}L^{-1}$ agar.

  • PDF

In Vitro Propagation of Cindium officinale Makino Through Shoot Tip Culture (천궁의 경정배양을 통한 기내번식)

  • 이현숙;정재동;김창배;윤재태;최부술
    • Korean Journal of Plant Tissue Culture
    • /
    • v.21 no.4
    • /
    • pp.221-225
    • /
    • 1994
  • This experiment was conducted to identify the optimal in vitro propagation condition of Cnidii rhizoma (Cnidium officinale Makino). It was effective to reduce contamination and improve regeneration of shoot when shoot tips taken in July were cultured in 1/2 strength Murashige and Skoog medium supplemented with 500 mg/L carbenicillin disodium 1.0 mg/L BA and 1.0mg/L $GA_3$followed by surface sterilization of explant source in solution of 1% sodium hypochlorite for 20 minutes. When shoot tips were 쳐cultured in 1/2 strength MS medium with 0.5 mg/L BA and 60 g/L sucrose, shoot elogation and subsequent multiplication of the formed shoot were favorable than in other media. Regenerants were well rooted in 1/2 strength MS medium containing 3.0 mg/L NAA.

  • PDF

Radioprotective Effect of Cyclo(L-Phenylalanyl-L-Prolyl) on Irradiated Rat Lung

  • Lee, Keyong-Ho;Rhee, Ki-Hyeong
    • Journal of Microbiology and Biotechnology
    • /
    • v.18 no.2
    • /
    • pp.369-376
    • /
    • 2008
  • In the present study, we investigated the radioprotective effect of cyclo(L-phenylalanyl-L-prolyl) on irradiated rat lungs to determine its potential as a radioprotective agent. We found that early lung damage induced by irradiation was reduced by treatment with 40mg/kg of cyclo(L-phenylalanyl-L-prolyl) in the latent and early pneumonitis phases. Expression of $TNF-{\alpha}\;and\;TGF-{\beta}1$ at 2 and TGF-${\beta}1$ at 8 weeks post-irradiation was decreased in animals that received both radiation and cyclo(L-phenylalanyl-L-prolyl) compared with animals that received radiation alone. Evidence indicated that the proinflammatory cytokine TNF-${\alpha}$ and the fibrogenic cytokine TGF-${\beta}1$ likely play a role in the radioprotective effect of cyclo(L-phenylalanyl-L-prolyl). However, besides TNF-${\alpha}$ and TGF-${\beta}1$ expressions, the precise mechanism by which cyclo(L-phenylalanyl-L-prolyl) ameliorates the induced radiation damage is not clear.