• International Journal of Industrial Entomology and Biomaterials
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• v.1 no.2
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• pp.131-135
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• 2000

The vitellin of the fireflies, Luciola unmunsana and L. lateralis was characterized. The vitellin of L. unmunfon is composed of two subunits, designated Vnl (195 kDa) and Vn2 (185 kDa) in SDS-polyacryamide gel electrophoresis. These two subunits of vitellin of L. unmunsana gradually decreased during embryogenesis. As expected, these protein bands were presented in female adult hemolymph and egg extracts, but not in male. The vitellin of L. lateralis is also composed of two subunits, designated Vnl (195 kDa) and Vn2 (180 kDa) in SDS-PAGEi and these two protein bands gradually decreased during embryogenesis. Western blot analysis using each of polyclonal antiserum against vitellins of L. unmunsana and L. lateralis showed that two antisera strongly crossereacted with vitellin subunits of L. unmunsana and L. lateralis, suggesting that vitellins of L. unmunsana and L. lateralis have similarity with each other.

• Korean Journal of Agricultural Science
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• v.50 no.3
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• pp.507-512
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• 2023

The traditional oviposition ground for indoor breeding of Luciola lateralis is moss. This study was conducted to find the most suitable alternative oviposition ground that can maintain or increase the oviposition rate of L. lateralis while addressing the problems of larva collection time and larva loss that occur when moss is used. As alternative candidate oviposition ground comprising six colors of non-woven fabric and felt were used to measure the fecundity variation rate of L. lateralis with respect to the color and material of the oviposition ground. In addition, measure the fecundity variation rate of L. lateralis in moss, a traditional oviposition ground, was also measured and investigated for comparison. The investigation showed that the average number of eggs in the non-woven fabric group was higher than that in the felt group. The yellow non-woven fabric had an average number of eggs that was more than 100 times higher than of moss used as a traditional oviposition ground. In the space where the six color non-woven fabric were together, L. lateralis concentrated its oviposition on yellow. These results showed that yellow non-woven fabric can be sufficiently used for efficient indoor mass breeding of L. lateralis while addressing problems caused by moss, a traditional oviposition ground.

• The Journal of Korean Medicine
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• v.23 no.1
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• pp.177-182
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• 2002

It is considered that herbal medicine may also have hepatotoxicity and Aconiti Radix Lateralis Preparata is thought to be the most poisonous. We investigated the changes of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and ${\gamma}-glutamyltranspeptidase{\;}({\gamma}-GT$)$ in 6 cases administrated herbal medicine containing Aconiti Radix Lateralis Preparata and tried to evaluate its influence on liver enzymes. Before administration, the mean values of AST and ALT were $30.5{\pm}12.4${\;}U/L{\;}and{\;}30.7{\pm}22.5{\;}U/L$. After administration, those of AST, ALT and ${\gamma}-GT{\;}were{\;}25.7{\pm}8.7 {\;}U/L,{\;}28{\pm}14.8{\;}U/L{\;}and{\;}34.3{\pm}19.4{\;}U/L$ respectively. Therefore, it is indirectly assured that herbal medicine containing Aconiti Radix Lateralis Preparata didn't influence liver enzymes such as AST, ALT, ${\gamma}-GT$.

• Journal of Forest and Environmental Science
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• v.34 no.4
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• pp.275-283
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• 2018

This study created the running water (Bandibooliwon) and lentic water (Sooryunwon) artificial habitats of Luciola lateralis in Hwadamsup, Gwangju-si, Gyunggi-do from April 2014 to February 2016. Through various habitat types, metapopulation was formed, and more sound population was induced to be maintained via interactions between habitats. We investigated coenosite at the habitat creation-target site, and 20 species in the lentic water site and 38 species in running water site were found. After releasing L. lateralis, we checked the status of settlement of L. lateralis larvae through traps and macroscopy, controlled the flow velocity of the habitats in line with the life cycle of L. lateralis (hatching, larva period, landing, pupation, copulation, and oviposition), and shaped gentle slope at water side, and planted moss. We artificially supplied melanian snails (Semisulcospira sp.), the food source, in the running water site and induced stable feeding. Also, We created artificial insectary, bred larvae stably in winter, and secured firefly population for a festival. Through night time observation, the time most suitable for a festival was confirmed to be from 20:30 to 22:00.

• International Journal of Industrial Entomology and Biomaterials
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• v.3 no.2
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• pp.141-147
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• 2001

To study the ecological characteristics of the Korean native firefly, Luciola lateralis, development and life history of L. lateralis were investigated throughout the indoor rearing. Average size of egg with an oval shape was 0.51${\times}$0.56 mm and an egg period of L. tateralis was approximately 25.8 days. The larvae were pupated at the 5$^{th}$ instar and the body length of the matured larvae was 15.8mm. The total periods of larval stage to the end of the $5^{th}$ instar were approximately 271.7 days. Pupae formed soil cocoon and average pupal period was approximately 28.7 days. Average number of eggs oviposited by a female was approximately 97. The body size of female in the pupa and adult were larger that of male.

• Journal of Biomedical Engineering Research
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• v.17 no.2
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• pp.221-226
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• 1996

Tendon reflex responses generated from mechanical stimulus had been studied for quantitative analysis of activity of tendon reflex, especially patellar tendon reflex responses are known to be a criterion for diagnosing the L3 or L4 radiculopathy. In this paper, we developed a digital signal processing system for analysis of the tendon reflex response. The system parameter, i.e., $\textit{sampling frequency, pre-amplification gain, input channel and filter bank}$ are selected by Using software switches. From the view points of flexibility, the system hardware is connected to an IBM PC for analyzing the tendon reflex parameters, amplitude, latency duration We applied the proposed system to the analysis of the patellar tendon reflex reponses. In the experiment, we measured latency, duration, amplitude of the reflex action potentials generated from vastus medialis, vastus lateralis and rectus femoris that compose quadriceps, and the measured data are analyzed througll the ANOVA test which has 5% significant level. As a result, we showed that the mean amplitude of reflex action potential at the vastus lateralis is larger than any other muscle and the mean latency of the reflex action potential at the rectus femoris is shorter than any other muscle.

• International Journal of Industrial Entomology and Biomaterials
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• v.1 no.1
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• pp.53-58
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• 2000

A cDNA encoding the luciferase of firefly Luciola lateralis was cloned downstream from the polyhedrin gene promoter of Bombyx mori nucleopolyhedrovirus and expressed in B. mori cells (BmN-4). The coding soquence for luciferase was inserted into pBmKSK2 rectors) which was reconstructed from the polyhedrin-based transfer vector pBmKSKl by modifying cloning sites. Recombinant virus, BmK2-LUCDF, containing the luciferase gene was selected and purified in BmN-4 cells. The emission of luminescence by luciferase was only detected in BmK2-LUCDF-infected cell extracts. This result indicates that the cloned new luciferase gene of firefly L. lateralis can be expressed efficiently in baculovirus expression system and used as a useful reporter gene.

• Korean Journal of Applied Biomechanics
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• v.17 no.2
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• pp.239-245
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• 2007

The purpose of this study was to compare the electromyography signal's power spectrum mean and median tendencies appearing in the lower extremity during walking while wearing roller shoes. 9 male subjects volunteered who have no experience riding inline-skate or roller-skate, and have no record of musculoskeletal disorder. Subjects walked on treadmill twice for an hour (Once a week, one trial with the roller on and the other without roller, Walking velocity = 1.39 m/s). Electromyography was measured every 15 minute (0, 15, 30, 45, 60 minutes). Surface electrode sticked muscle at rectus femoris(R.F.), vastus lateralis(V.L.), vastus medialis(V.M.), biceps femoris(B.F.), tibialis anterior(T.A.), gastrocnemius lateralis(G.L.), gastrocnemius medialis(G.M.). At Rectus femoris, Vastus Lateralis, Vastus medialis, and Biceps femoris showed no statistically significant decrease of median frequency or mean edge frequency as time passes. Also, between two treatments (wearing the roller shoes vs not wearing the roller shoes), no statistically significant difference. After 60 minutes, mean edge frequency showed statistically significant decrease at tibalis anterior and after 45 minutes, mean edge frequency showed statistically significant decrease compared to wearing roller shoes without the wheels at gastrocnemius lateralis. At gastrocnemius medialis after 30 minutes, median frequency showed statistically significant decrease, and showed statistically significant difference compared to the control group. Wearing the roller shoes with wheels for a long time resulted in statistically significant decrease of mean edge frequency and median frequency in lower extremity, especially in shank muscles. Increase of wearing time of roller shoes and walking on a bumpy road wearing roller shoes with wheels result fatigue and thus, danger of injury.

• Korean journal of applied entomology
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• v.47 no.3
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• pp.265-272
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• 2008

Toxicity of 10 registered insecticides and 6 fertilizers were tested against $3^{rd}$ larva and adults of Korean firefly, Luciola lateralis Motschulsky(Coleoptera: Lampyridae). All experiments were tested at the recommended concentration of each inescticides by producer. MEP, PAP, Acephate, Fenthion, and Diazinon, which were organophates, a mixtures combined with Burofezin fenobucarb, Cartap buprofezin, and Thiamethoxam(Neonicotinoids), Fipronil(Phenylpyrazoles) showed more 80.0% mortality on larva and adults of L. lateralis. However, tebufenozide(I.G.R) showed low mortality of 33.3%. $LC_{50}$ (ppm) value of Assit, Cartap buprofezin, Fenthion and PAP were showed 1.03 ppm, 1.90 ppm, 10.26 ppm, 0.98 ppm, respectively, against $3^{rd}$ larva of L. lateralis. Effects against eggs showed very high toxicity. Otherwise, tebufenozide(I.G.R) was showed hatchability of 100%. Toxicity of Urea fertilizer, Ammonium sulfate, Potassium chloride, Fused phosphate, Complex fertilizer and Silicate fertilizer were showed the mortality with 27.3%, 56.7%, 73.3%, 0.0%, 0.0%, 0.0%, respectively, when exposed 72 hrs after treatment.

• Korean journal of applied entomology
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• v.48 no.2
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• pp.197-202
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• 2009

The Luciola lateralis larva took 5.3 days from climbing on the land to the pupal cocoon formation. It took 6.6 days for a larva to eventually transform to a pupa after building a pupal cocoon. The size of pupal cocoon was 10.1 mm in length, 4.7 mm in width and its wall thickness was 1.3 mm. The mean pupal period was 10.5 days. The adult stayed 6.8 days in the pupal cocoon before escaping the cocoon. The peak adult emergence appeared around 9 p.m., and decreased after 10 p.m. The optimal soil temperature for emergence was $23.4^{\circ}C$. The female adult of the natural population (Nat-type) lived shorter, laid fewer eggs, and the oviposition frequency was fewer than that of the Lab-type individuals. However, a few individuals from the natural population laid 200-400 eggs. The less number of oviposition in the natural population may be due to the fact that the female adults might lay eggs before the collection for the experiment.