• Title/Summary/Keyword: L., biodegradation

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Sewage Disposal by Different Structure of Fluidized Bed Biofilm Reactor (유동층 생물반응기의 구조변화에 따른 하수처리)

  • Park, Jong-Man;Lee, Jae-Yong;Kim, Chul-Kyoung;Koh, Chang-Woong;Kim, Nam-Ki
    • Journal of Korean Society of Water and Wastewater
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    • v.18 no.2
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    • pp.181-187
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    • 2004
  • The purpose of this study is to investigate the biofilm reactors capable of doing high efficiency treatment. Vertical fluidized bed biofilm reactor(VFBBR) and spiral fluidized bed biofilm reactor(SFBBR) was used for their performence in biodegradation of artificial sewage. The factors influencing the efficiency of those reactors were compared with difference of physical condition. They had same size but different structure to gain access of its unique characteristics. When recycle solution with flow rate of 22 mL/min and artificial sewage with flow rate of 2~10 mL/min were fed into two reactors in aerobic state, the average $COD_{cr}$, removal rate for biodegradation of SFBBR was greater than VFBBR. After reactor feed sewage was constantly maintained as flow rate of 4 mL/min and the recycle solution were changed to 10~32 mL/min respectively, the average $COD_{cr}$ removal rate of artificial sewage in SFBBR was greater than VFBBR. In this experiment for addition of support media into two reactors SFBBR was 4.1% excellent than VFBBR. Above all, SFBBR excelled VFBBR in boidegradation of organic matter in sewage.

Polyvinyl Alcohol Degradation by Microbacterium barkeri KCCM 10507 and Paenibacillus amylolyticus KCCM 10508 in Dyeing Wastewater

  • Choi, Kwang-Keun;Park, Chul-Hwan;Kim, Sang-Yong;Lyoo, Won-Seok;Lee, Sang-Hun;Lee, Jin-Won
    • Journal of Microbiology and Biotechnology
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    • v.14 no.5
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    • pp.1009-1013
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    • 2004
  • The purpose of this study was to investigate the degradation of PVA (polyvinyl alcohol) contained in dyeing wastewater by a mixed culture of Microbacterium barkeri KCCM 10507 and Paenibacillus amylolyticus KCCM 10508. Firstly, synthetic wastewater which contained different initial concentrations of PVA varying from 50 to 3,500 mg/l were tested to obtain optimal PVA biodegradation activity of isolated strains, and the above two strains were found to degrade PVA up to 90%, when the initial concentration of PVA was 750 mg/l and below. Next, dyeing wastewater was tested by a nixed culture of the two isolated strains, and 42% and 55% of the initial concentrations of PVA and COD, respectively, was removed after five days. MLSS was gradually increased from an initial 1,400 to 2,500 mg/l, and the pH was also increased from 5.1 to 7.8. Sterilized dyeing wastewater was tested to find the effect of strains only on the biodegradation of PVA, and PVA degradation ratio and COD removal ratio were 50% and 72.8%, respectively. Thus, the results indicated that these two strains have good ability to degrade PVA and remove COD in dyeing wastewater, Finally, it is expected that if these two strains were used in the dyeing wastewater treatment, good efficiency for PVA degradation and COD removal could be achieved.

Isolation of a Poly (L-lactide) Degrading Bacterium and Improvement of its Degradation Capacity (Poly(L-lactide)분해 세균의 분리 및 활성 증진)

  • Kim, Mal-Nam;Park, Sang-Tae
    • Korean Journal of Environmental Biology
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    • v.25 no.3
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    • pp.260-266
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    • 2007
  • A thermophilic bacterium capable of poly (L-lactide)(PLLA) degradation was isolated from cultivating soil in Korea. The isolate was Gram positive rod-shaped bacterium, and was identified as Geobacillus caldoxylosilyticus based on the 16S rDNA sequence analysis. The strain proved to be a new PLLA degrading bacterium which has not been reported in the open literatures yet. The degradation activity of the strain was assessed in a sterilized compost inoculated with the strain under controlled compost condition at $58^{\circ}C$ for 40 days. The strain mineralized 66%, 57%, 41% and 40% of PLLA5000, PLLA11000, PLLA34000 and PLLA256000 whose weight average molecular weights were 5000, 11000, 34000 and 256000, respectively. Incorporation of 0.1% each of gelatin, yeast extract and ammonium sulfate in the compost containing PLLA256000 as a nutritional supplement raised the biodegradation activity by 27%, 13% and 10%, respectively. Increase of the inoculum size from $10^9cfu\;g^{-1}\;to\;10^{10}cfu\;g^{-1}\;and\;10^{11}cfu\;g^{-1}$ also enhanced the biodegradation activity by 14% and 20%, respectively.

Biodegradation of Polychlorinated Biphenyls (PCBs) within Insulating Oil by Pseudomonas sp. P2 (Pseudomonas sp. P2에 의한 절연류 내의 Polychlorinated Biphenyls (PCBs)의 분해)

  • Kim, Jung-Ho;Choi, Sang-Ki;Kim, Young-Ho
    • Journal of Environmental Health Sciences
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    • v.22 no.3
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    • pp.1-7
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    • 1996
  • Polychlorinated Biphenyls(PCBs)의 생물학적 처리가 시도되고 있으며, PCBs를 분해할 수 있는 미생물을 이용할 수 있다. 따라서 본 연구에서는 폐기된 절연유의 생물학적 처리를 위하여 PCBs를 분해하는 균을 분해하였으며, 분해된 균을 이용하여 절연유 내의 Polychlorinated Biphenyls(PCBs) 분해를 회분식 실험에서 연구하였다. 대구의 신천으로부터 유일한 탄소원으로 Biphenyl을 포함하고 있는 고체배지에서 PCBs를 분해할 수 있는 Pseudomonas sp. P2 균주를 분해하였다. PCBs의 용해도를 높이기 위해 사용된 유화제 alkyl aryl ethoxylated phosphate가 200 mg/L에서는 Pseudomonas sp. P2 균주의 성장에 영향을 미치지 않았다. 1000 mg/L의 Biphenyl과 PCBs에 Pseudomonas sp. P2를 접종하여 160시간 배양후에 Biphenyl과 PCBs의 분해가 각각 97.5%, 58.0%였다. Biphenyl 1000 mg/L에서 최대성장율($\mu_{max}$)은 0.34 $day^{-1}$, 0.26 였다. 따라서 염소가 결합되지 않은 Biphenyl는 염소가 결합된 PCBs보다 분해가 빠르게 진행되었다. 또한 Pseudomonas sp. P2는 Biphenyl과 PCBs의 분해로 부터 유도된 황색의 분해대사산물을 확인하였다. 본 연구에서는 Pseudomonas sp. P2 균주가 절연유 내의 PCBs를 분해할 수 있다는 것을 확인하였다.

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Synthesis and Evaluation of Copoly(chitosan-g-L-lysine) for Wound Covering Material (Chitosan과 L-lysine의 공중합체로 부터 인공피부의 제조와 평가)

  • Kim, K.Y.;Min, D.S.;Park, S.H.;Lee, S.Y.;Cho, Y.J.;Chung, Y.H.;Kim, J.M.
    • Proceedings of the KOSOMBE Conference
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    • v.1989 no.05
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    • pp.7-8
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    • 1989
  • The graft copolymer of chitosan with amino acid, L-lysine was synthesized by heterogeneous copolymerization and was evaluated as an artificial skin. The mechanical properties under dry and wet state, water content, water vapor transmittance rate and biodegradability were measured. The tensile strength and elongation under wet state ranged $0.3-0.5\;kg/mm^2$, 10-13%, respectively. Water vapor transmittance rate ranged $450-500\;g/m^2{\cdot}day$ like that of the normal skin. The weight loss of prepared membrane by protease IV was measured for the degree of biodegradation. The degree of biodegradation was around 15% and after 4 days it was slow. Biocompatibility was evaluated by studying the attachment of human fibroblast on the prepared membrane surface.

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Biodegradation of a Reactive Dye, Remazol Black B in a UASB Reactor (UASB 반응기를 이용한 반응성 염료 Remazol Black B의 분해)

  • Oh, You-Kwan;Lee, Sung-Ho;Kim, Hyo-Seob;Kim, Yu-Jin;Lee, Sang-Joon;Park, Sung-Hoon
    • KSBB Journal
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    • v.14 no.6
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    • pp.688-695
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    • 1999
  • Biodegradation of the reactive dye, Remazol Black B was investigated in an upflow anaerobic sludge blanket(UASB) reactor. Important parameters studied include dye concentration(20-60 mg/L), glucose concentration as a co-substrate(1,000-3,000 mg/L), hydraulic retention time(3-24 hr), and influent pH(6.0-8.0). Under most conditions tested, the molecules of Black B were degraded readily and completely according to HPLC chromatograms. However, the color removal efficiency based on spectroscopic measurement was always approximately 75%. This suggests that the degradation products have some color intensity corresponding to 25% of the original dye molecules. The maximum influent dye concentration which satisfies the legal discharge limit of color intensity of 400 ADMI was 13 mg/L. and the highest removal rate at this dye concentration was 104 mg/L${\cdot}$day.

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Creation of Bio-Inspired Fiber Materials and Their Biodegradation

  • Ohkawa, Kousaku;Yamamoto, Hiroyuki
    • Proceedings of the Korean Fiber Society Conference
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    • 2003.10a
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    • pp.43-44
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    • 2003
  • The new method for preparing hybrid fibers from aqueous solution is described. The method is based on interfacial polyionic complexation between the counter-charged polymers. Polysaccharides, chitosan and gellan, and polypeptides, poly(L-lysine) and poly(L-glutamic acid) were utilized as the components of the fibers. The chitosan-gellan and poly(L-lysine)-gellan hybrid fibers exhibited a high level biodegradability.

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Nitroglycerin Biodegradation under Denitrification Conditions and Corresponding Microbial Community Shifts upon Acclimation (탈질조건에서 nitroglycerin의 생물학적 분해 동역학 및 미생물 군집 변화)

  • Choi, Wonchul;Bae, Bumhan
    • Journal of Soil and Groundwater Environment
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    • v.24 no.5
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    • pp.42-54
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    • 2019
  • Biodegradation of an explosive compound, glyceryl trinitrate (GTN), was studied with a denitrifying microbial culture grown in a sequencing batch reactor and a GTN acclimated denitrifying culture. The GTN acclimated culture, which were fed on GTN for 1 month, degraded GTN regioselectively via denitration on C1 position as compared to C2 position denitration by denitrifying culture that has never been exposed to GTN. Accumulation of two isomeric glyceryl dinitrates (GDNs) in both culture medium suggests that GDN denitration is the rate-limiting step in GTN biodegradation. The first order GTN degradation rate normalized to cell concentration of the acclimated culture was calculated to be 0.045 (${\pm}0.002$) L/g-hr. Increasing concentration of electron acceptor(nitrate) resulted in discouraged GTN degradation. According to microbial community analysis, prolonged GTN exposure resulted in 25% increase in the genus level of the GTN acclimated culture with the disappearance of two dominating denitrifying microbial species of Methyloversatilis universalis and Hyphomicrobium zavarzinii in the denitrifying culture.