• 한국미생물·생명공학회지
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• 제15권5호
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• pp.361-367
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• 1987

DL-seleno-methionine에 내성을 갖는 변이주, Cephalosporium acremonium MS-92는 무기황과 메치오닌의 Uptake rate가 모균보다 우수하였으며, 세포내에 과량의 메치오닌과 S-adenosylmethionine (SAM)을 축적하였다. 또 C. acremonium에서 무기황의 uptake rate는 시스테인에 의해 크게 저해되었다. Crude enzymes extracts에서 변이주 MS-92 는 cell내에 cysteine을 적게 형성하였다 즉, L.-serine sulfhydrylase 활성이 모균보다 낮았으며 세포내에 시스테인의 양을 간접적으로 알 수 있는 시스테인 분해효소인 cysteine desulfhydrylase 활성치 변이주에는 모균의 약 반이였다. 이상의 결과에서 C. acremonium에서 메치오닌을 만드는 효소들의 조절에는 A. nidulans와 비슷하게 시스테인이 관여한다는 것을 추측할 수 있었다.

• 미생물학회지
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• 제25권3호
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• pp.205-211
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• 1987

Cephalosporium acremonium MAR-80, a strain of methionine analogue-resistant mutant, showed good activity of sulfate utilization as only sulfur source. The effect of methionine on the sulfate uptake system was investigated by using $Na_{2}^{35}SO_{4}$ as a tracer in the resting cell system. From this result, it was revealed that sulfate permease of this strain was less repressed and/or less inhibited by methionine than parent type. This deregulation was due to low actibity of methionine uptake, which was operated by somewhat simple diffusion. From these studies, it could by anticipated that the improved productivity of cephalosporin C and lower dependence of cephalosporin C production on methionine were related to increased uptake rate of sulfate.

• 한국초지조사료학회지
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• 제10권3호
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• pp.141-146
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• 1990

Changes in dry matter yield, crude components, nutrients uptake($P_2O_5$, $K_2O$, CaO, MgO) and sulphur containing amino acid(cysteine, methionine) of orchardgrass(Dacty1is glomerata. L) and alfalfa(A4edicago sativa. L) by gypsum application(as sulphur source, 0, 2. 5, 10, 20kg SIlOa) were investigated to understand the effect of sulphur on herbage production in pasture, which was established in 1987 as means of hand broadcasting. The effect of gypsum on dry matter yield at different cutting times during growing seasons has not been found both in orchardgrass and in alfalfa, but in respect to annual total dry matter yield there were increment in herbage yield (P<0.05) of alfalfa at 5, 10, 20kg SIlOa in 1989 and the amounts of sulphur taken up in herbage slightly increased according to the rates of gypsum application. Maximum apparent recovery of sulphur was 7.55% at 2kg SIlOa in orchardgrass and was 17.8% at 5kg S/lOa in alfalfa. There were no any great differences in the content of crude components of both species and this trend was similar with the mineral contents of orchardgrass. But in alfalfa, the amounts of $K_2O$, CaO, and $P_2O_5$ taken up were increased by gypsum application and the increment in the amounts of minerals taken up in herbage at 20kg SIlOa were 14.9 of $K_2O$, 9.1 of CaO, and 2.5kgIlOa of $P_2O_5$ as compared to those of at untreated plot. Cysteine and methionine were not influenced by gypsum applicaton not only in orchardgrass but also cysteine in alfalfa, however, the content of methionine in alfalfa was slightly increased at 2, 5, lOkg SIlOa and at 20kg SIlOa was reverse.

• Applied Biological Chemistry
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• 제43권3호
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• pp.163-168
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• 2000

토양 및 수계에 집적, 유입되어 있는 과다한 인산의 제거에 이용 가능한 미생물 자원을 확보하기 위하여 활성오니에서 분리된 Acinetobacter lwoffi PO8의 배양조건 및 외부환경조건에 따른 인산흡수 양상을 조사하였다. 배양액 내 초기 pH가 $7.5{\sim}8.5$ 범위에서 균생육과 인산흡수율이 가장 높았으며, 탄소원으로 glycerol 및 arabinose을 첨가하였을 경우 각각 약 93 및 91%의 높은 인산 흡수율을 보였다. 질소원 형태에 따른 인산흡수양상은 아미노태 보다 암모늄염이 효과적이었으며, 특히, $NH_4NO_3$,와 $(NH_4)_2SO_4$가 각각 95와 96%의 인산흡수율을 나타냈다. 금속이온 중에서 $Co^{2+}$ 첨가 시 균생육이 제해되었으나, 이외의 다른 금속이온은 균의 성장 및 인산흡수에 큰영향을 미치지 않았다. 아미노산 중 arginine, methionine 및 lysine 등은 아미노산을 첨가하지 않은 경우보다 $10{\sim}20%$ 더 높은 인산흡수율을 나타냈었으며, 이때 배양기간 중 glucose의 공급으로 배지 중 잔존 인산이 완전히 제거되었다. 또한 $^{32}P$를 사용하여 균의 인산 분포를 조사한 결과로 세포내 흡수된 인산은 대부분 세포의 원형질 내에 polyphosphate의 형태로 분포되어 있었다.

• Journal of Korean Neurosurgical Society
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• 제39권3호
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• pp.176-182
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• 2006

Objective : This study concernes the usefulness of $^{11}C-methyl-L-and$ D-methionine[Met]-positron emission tomography[PET] for glioma grading and detection of recurrence in gliomas, compared with fluorine-18, 2-fluoro-deoxyglucose[FDG]-PET. Methods : Eighty patients underwent Met-PET study for evaluation of glioma : 37 astrocytomas [WHO grade II, 3; III, 8; IV, 26]. 27 oligodendrogliomas [WHO grade II, 16; III, 11]. and 12 suspicious recurrent gliomas. All images were taken within 2 weeks before operation. For suspicious recurrent cases on magnetic resonance images, both FDG-PET and Met-PET were performed. Results : In astrocytoma, Mean maximum standard uptake value[SUV] of region of interest[ROI] was not different between WHO grades [p=0.108]. but ROI/normal contralateral tissue SUV [T/N] ratio was statistically different between WHO grades [p=0.002]. T/N ratio was more closely related to visual scale than maximum SUV of ROI [p<0.001 and p=0.107 respectively]. In oligodendroglioma, there was no statistical difference between WHO grades in view of maximum SUV and T/N ratio. For recurrent gliomas, sensitivity of FDG-PET and Met-PET was 25% and 100%, while specificity of FDG-PET and Met-PET were 100% and 80%, respectively. Conclusion : Met-PET might be an appropriate tool for tumor grading in astrocytoma and be more sensitive for detection of recurrence in gliomas than FDG-PET.

• The Korean Journal of Physiology and Pharmacology
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• 제20권5호
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• pp.449-457
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• 2016

N-acetyl-L-cysteine (NAC) and cysteine have been implicated in a number of human neutrophils' functional responses. However, though $Ca^{2+}$ signaling is one of the key signalings contributing to the functional responses of human neutrophils, effects of NAC and cysteine on intracellular calcium concentration ($[Ca^{2+}]_i$) in human neutrophils have not been investigated yet. Thus, this study was carried out with an objective to investigate the effects of NAC and cysteine on $[Ca^{2+}]_i$ in human neutrophils. We observed that NAC ($1{\mu}M{\sim}1mM$) and cysteine ($10{\mu}M{\sim}1mM$) increased $[Ca^{2+}]_i$ in human neutrophils in a concentration-dependent manner. In NAC pre-supplmented buffer, an additive effect on N-formyl-methionine-leucine-phenylalanine (fMLP)-induced increase in $[Ca^{2+}]_i$ in human neutrophils was observed. In $Ca^{2+}$-free buffer, NAC- and cysteine-induced $[Ca^{2+}]_i$ increase in human neutrophils completely disappeared, suggesting that NAC- and cysteine-mediated increase in $[Ca^{2+}]_i$ in human neutrophils occur through $Ca^{2+}$ influx. NAC- and cysteine-induced $[Ca^{2+}]_i$ increase was effectively inhibited by calcium channel inhibitors SKF96365 ($10{\mu}m$) and ruthenium red ($20{\mu}m$). In $Na^+$-free HEPES, both NAC and cysteine induced a marked increase in $[Ca^{2+}]_i$ in human neutrophils, arguing against the possibility that $Na^+$-dependent intracellular uptake of NAC and cysteine is necessary for their $[Ca^{2+}]_i$ increasing activity. Our results show that NAC and cysteine induce $[Ca^{2+}]_i$ increase through $Ca^{2+}$ influx in human neutrophils via SKF96365- and ruthenium red-dependent way.

• 한국환경농학회지
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• 제40권1호
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• pp.33-39
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• 2021

BACKGROUND: Globally, large amounts of phosphogypsum (PG), which is a by-product of the phosphate fertilizer production, is deposited in open areas. As PG contains calcium, phosphate, and sulphate, it can be used as a soil amendment in farmlands. This study was conducted to investigate the effects of PG application on properties of field soil and yield and quality of garlic (Allium sativum L.), and to seek appropriate level of PG application into the field. METHODS AND RESULTS: This experiment was conducted by applying PG at four different levels that were adjusted based on 65% calcium base saturation in the field soil: 0% (control), 50% (PG50, 100 kg/10a), 100% (PG100, 200 kg/10a), and 150% (PG150, 300 kg/10a). Following cultivation, soil electrical conductivity (EC), organic matter, available P and exchangeable Ca increased, whilst soil pH decreased. With increase in PG application level, soil EC and exchangeable Ca increased. PG application increased concentrations of water soluble Ca and SO4 across the soil profile, especially in PG150. The highest yield of garlic was found in PG100 treatment. The plant's uptake for N, P, Ca, and S increased by PG applications, but that for K decreased. Moreover, concentrations of S-related amino acids such as cysteine and methionine in garlic increased by PG applications. The increased content of nutrients and amino acids with PG supply might improve yield, quality, and favor of the crop. CONCLUSION: Overall, PG application at 200 kg/10a into a field had the best effect on improving soil fertility as well as yield and quality of garlic. Further studies are required to maximize efficiencies of PG supply in soil management and production of various crops.