• 대한수의학회지
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• 제29권4호
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• pp.461-467
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• 1989

The activity of lactate dehydrogenase in plasma and various tissues(skeletal muscle, cardiac muscle, liver, lung, kidney and spleen) of Korean native cattle in a Chonju abattoir, the Breeding Stock Farm and Animal Farm of Chonbuk University was determined by using ultra violet method. Using polyacrylamide gel electrophoresis, the lactate dehydrogenase isoenzyme distrimution of plasma and various tissues in Korean native cattle was studied. The plasma lactate dehydrogenase activity of Korean native cattle was $554.80{\pm}92.70IU/l$ and the lactate dehydrogenase activity of male plasma was $543.96{\pm}97.89IU/l$, which was lower than that of female plasma, $579.19{\pm}78.09IU/l$. The plasma lactate dehydrogenase activity of calf was $557.31{\pm}110.27IU/l$ and was no significantly different from that of adult Korean native cattle. But the range of calf lactate dehydrogenase activity was larger than that of adult Korean native cattle. In tissues, the lactate dehydrogenase activity was decreased in order of lung, kidney, spleen, liver, heart and skeletal muscle. The lung had the greatest activity and the skeletal muscle had the least. Lactate dehydrogenase isoenzymes in plasma and tissues were found to have a characteristic distribution and quantitative isoenzyme patterns. In plasma, the LDH1 usually had the greatest activity and other isoenzymes showed a decreasing tendency in order of LDH2, LDH3, LDH4 and LDH5. The distribution of lactate dehydrogenase isoenzymes had a wide variation in tissues. But the distribution of LDH isoenzymes in plasma was similar to that in kidney, and also cardiac muscle and spleen had similar pattern in LDH isoenzymes distribution.

• KSBB Journal
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• 제11권4호
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• pp.489-496
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• 1996

Carbon paste electrode를 채용함으로써 L-lac tate 측정용의 전기화학식 바이오센서를 성공적으로 개발할 수 있었다. 특히 뛰어난 electrocatalytic activity를 갖는 platinum이 첨가된 platinum-CPE 를 이용하여 낮은 전위에서의 NADH의 전기척 산 화가 가능하였다. Enzyme (lactate dehydrogen­a ase)과 $NAD^+$를 carbon paste형식으로 직접 제조 함으로써 L-lactate 측정을 위한 성공적인 바이요센 서의 개발이 가능하였다. 위와 같은 개발연구를 통 하여 다른 $NAD^+$ -dependent dehydrogenase를 채 용한 바이오센서로의 적용이 기대된다.

• 한국식품영양과학회지
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• 제23권6호
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• pp.964-972
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• 1994

The 4.3-kb gene coding for L-lactate dehydrogenase of Bacillus stearothermophilus has been subcloned and expressed in E. coli cells. The enzyme was purified 200-fold with 25% yield by heat treatment , DEAE-Sephadex, and NAD++ -Sepharose CL-4B affinity chromatography followed by gel filtration through Sephadex G-200 . The molecular weight of the purfied enzyme was estimated to be about 35, 000 and 140, 000 on SDS-polyacrylamide gel electrophoresis and gel filtration, respectively. indicating that the enzyme is composed of four identical subunits. THe enzyme for pyruvate reduction and lactate oxdiation was stable at 60 and 75$^{\circ}C$ for 30 min, and the optimal temperatures for both reactions were 60 and 7$0^{\circ}C$, respectively. The enzyme had an optimal pH at 5.5 and 8.5 in pyruvate reduction and lactate oxidation, respectively. The pH stability of enzyme of pyruvate reduction was table between pH 5 and 7. more than 90% of enzyme activity was lost at 1mM FeSO4 and p-chloromercuribonzoate. The maximal activation of the enzyme was obtained with 0.8mM fructose 1, 6-bisphosphate.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권4호
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• pp.318-322
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• 2004

Lactic acid is an environmentally benign organic acid that could be used as a raw material for biodegradable plastics if it can be inexpensively produced by fermentation. Two genes (ldhL and ldhD) encoding the L-(+) and D-(-) lactate dehydrogenases (L-LDH and D-LDH) were cloned from Lactobacillus sp., RKY2, which is a lactic acid hyper-producing bacterium isolated from Kimchi. Open reading frames of ldhL for and ldhD for the L and D-LDH genes were 962 and 998 bp, respectively. Both the L(+)- and D(-)-LDH proteins showed the highest degree of homology with the L- and D-lactate dehydrogenase genes of Lactobacillus plantarum. The conserved residues in the catalytic activity and substrate binding of both LDHs were identified in both enzymes.

• Journal of Yeungnam Medical Science
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• 제12권1호
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• pp.48-55
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• 1995

1995년 1월에서 1995년 3월까지 심근 손상이 의심되는 환자 및 정상대조군에 대한 TnT, 총 LD, 총 CK 및 각 동위효소에 대한 고찰에서 다음과 같은 결과를 얻었다. Tropohin T는 정상인에서 $0.01{\pm}0.02{\mu}g/L$, 급성심근경색환자에서 최고치는 4.7-24.2 ${\mu}g/L$로 나타났으며, 초기검사에서 정상범위에 속한 환자에서 6시간 후 추적조사에서는 모든 증례에서 1.0 ${\mu}g/L$이상으로 나타났다. 총 LD는 1-3일경에 최고치를 보인 후 점차적으로 감소되었으나, LD1/LD2 비율은 대부분의 환자에서 10일 이상 1.0이상으로 유지되므로, 상대적으로 늦은 시간에 내원한 경우 총 LD와 LD1/LD2 비율이 진단에 유용할 것으로 사료되었다. 총 CK 및 CK-MB인 경우 대부분의 환자에서 3-4일 후 정상 범위로 떨어지므로, 조기진단에는 도움이 되나, 장기간의 추적조사에는 유용성이 없는 것으로 사료되었다. 총 Lactate Dehydrogenase, LD1/LD2 비율, 총 Creatine Kinase, CK-MB와 Troponin T의 상호비교에서 Troponin T가 상대적으로 초기 혹은 장기간 경과된 경우에도 유용한 지표로 사료되었다.

• 미생물학회지
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• 제46권2호
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• pp.213-218
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• 2010

Lactate dehydrogenases (LDHs)는 세포 내의 생화학적 대사경로에서 중요한 역할을 담당하는 효소로서 오랜 동안 많은 관심을 받았다. 본 연구에서는 다양한 미생물 genome database의 탐색을 통해 Bacillus cereus ATCC 14579 genome으로부터 LDH로 추정되는 3종의 유전자를 발견하고, 대장균에서 클로닝 및 대량 발현하였다. 모든 BcLDH 동질효소들의 상동부위 아미노산 잔기 대부분이 기존의 $NAD^+$-의존형 LDH와 높은 상동성을 보였다. 한편 314개의 아미노산으로 이루어진 BcLDH1과 2는 86%의 서열 상동성을 보였으나, BcLDH3와는 49%의 상동성을 나타냈다. 흥미롭게도 BcLDH1만이 $NAD^+$ 조효소 존재 하에서 L-lactate와 pyruvate 간의 상호전환 활성을 나타냈으며, 그 외의 동질효소들은 거의 활성을 보이지 않았다. 결론적으로 BcLDH1은 전형적인 $NAD^+$-의존형이며, L-lactate에 특이적인 dehydrogenase 효소임을 확인하였다.

• BMB Reports
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• 제32권2호
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• pp.140-146
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• 1999

When murine fibrosarcoma L929 cells, a TNF-sensitive cell line, were treated with recombinant human tumor necrosis factor-$\alpha$ (rhTNF-$\alpha$), the activities of glycolytic regulatory enzymes and lactate dehydrogenase increased up to 100-150% compared to the control L929 cells after TNF treatment. By using various metabolic inhibitors and activators, it was found that cAMP-dependent protein kinase is responsible for the increase of activities of the glycolytic enzymes. The activities of glycolytic regulatory enzymes and lactate dehydrogenase of TNF-resistant A549 cells, a human lung carcinoma cell line, did not increase significantly compared to TNF-sensitive L929 cells upon TNF treatment. In contrast, the pyruvate carboxylase activities of A549 cells, but not L929 cells, increased up to 30~40% after TNF treatment. The data suggest that pyruvate carboxylase activity may contribute to the compensation of energy loss mediated by TNF treatment in TNF-resistant A549 cells.

• Journal of Applied Biological Chemistry
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• 제56권1호
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• pp.1-3
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• 2013

본 연구결과에서 홍경천 추출물은 L6 근육세포에서 산화적 손상으로 인한 세포사멸과 ATP 생성량 감소를 유의적으로 억제하였을 뿐만 아니라 마우스에 2주간 200 mg/kg 농도로 경구투여시 통계적으로 유의하지는 않았으나 treadmill 운동시간을 증가시켰고 glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, lactate dehydrogenase, creatinine, lactate 등의 혈액지표를 개선시켰다. 따라서 홍경천은 과도한 운동 스트레스로 인한 손상을 억제할 수 있는 소재로써의 큰 가능성을 가지고 있는 것으로 판단된다.

• Journal of Periodontal and Implant Science
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• 제22권2호
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• pp.214.2-214.2
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• 1992

• Journal of Microbiology and Biotechnology
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• 제29권3호
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• pp.392-400
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• 2019

Chondroitin, the precursor of chondroitin sulfate, which is an important polysaccharide, has drawn significant attention due to its applications in many fields. In the present study, a heterologous biosynthesis pathway of chondroitin was designed in a GRAS (generally recognized as safe) strain C. glutamicum. CgkfoC and CgkfoA genes with host codon preference were synthesized and driven by promoter Ptac, which was confirmed as a strong promoter via GFPuv reporter assessment. In a lactate dehydrogenase (ldh) deficient host, intracellular chondroitin titer increased from 0.25 to 0.88 g/l compared with that in a wild-type host. Moreover, precursor enhancement via overexpressing precursor synthesizing gene ugdA further improved chondroitin titers to 1.09 g/l. Chondroitin production reached 1.91 g/l with the engineered strain C. glutamicum ${\Delta}L-CgCAU$ in a 5-L fed-batch fermentation with a single distribution $M_w$ of 186 kDa. This work provides an alternative, safe and novel means of producing chondroitin for industrial applications.