• Asian-Australasian Journal of Animal Sciences
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• v.14 no.2
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• pp.237-242
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• 2001

Colostrum deprived, newborn pigs (N=12, $1.64{\pm}0.05kg$) were used to study the renal threshold of carnitine, and effects of emulsified medium-chain triglyceride (MCT, tri-8:0) feeding on kinetics of plasma carnitine and urinary carnitine excretion. An arterial catheter was inserted through an umbilical artery, and a bladder catheter was inserted via the urachus. Piglets were oro-gastrically gavaged with one of six carnitine levels (0, 60, 120, 180, 240, $480{\mu}mol/kg\;W^{0.75}$) with (+MCT) or without medium-chain triglycerides (-MCT) in 0.9% NaCl solution. Blood was sampled into heparinized tubes at 0, 1, 2, 4, 6, 8, 14, and 20 h after gavage, and urine was collected and pooled into 1 h or 2 h composite samples to determine free- and short-chain carnitine concentrations. Plasma from the 12 newborn piglets before gavage contained $10.6{\pm}1.2{\mu}mol/L$ free carnitine and $7.2{\pm}0.6{\mu}mol/L$ acid-soluble acyl carnitine. The renal threshold for carnitine was similar between the MCT and the +MCT group (42.6 13.1 and $46.4{\pm}2.0{\mu}mol/L$, respectively), but the correlation between plasma free carnitine and urinary excretion was altered. Plasma free carnitine linearly increased with increasing carnitine dosage (-MCT group, $R^2=0.95$, p<0.001; +MCT group, $R^2=0.91$, p<0.001), but was decreased by 50% when medium-chain triglycerides were fed. The peak in plasma free carnitine concentration was depressed by medium-chain triglycerides feeding also. Therefore, the plasma and urinary short-chain/free carnitine ratio of the +MCT group was increased by 100% and 40%, respectively (p<0.01). Feeding of medium-chain triglycerides may delay plasma carnitine elevation via altering the kinetics of absorption. Similarly, the plasma and urinary short-chain/free carnitine ratio were affected by interaction between medium-chain triglycerides and time (p<0.01). The present study suggests that an oral carnitine dose over $480{\mu}mol/kg\;W^{0.75}$ may be needed to reach the free carnitine renal threshold within a short period, especially when provided together with medium-chain triglyceride.

• Asian-Australasian Journal of Animal Sciences
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• v.9 no.6
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• pp.701-703
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• 1996

The influence of phenylalanine (Phe) in the medium on protein synthesis of chicken embryo fibroblasts (CEF) was examined. CEF was derived from 9-d-old embryos by trypsin-EDTA digestion. To examine the deficiency of Phe in the medium, CEF was cultured in Dulbecco's modified Eagle's medium (DMEM) with or without Phe. CEF was also cultured in Dulbecco's phosphate buffered saline (PBS ($Ca^{2+}$, $Mg^{2+}$)) with or without $400{\mu}m$ Phe in order to examine the effect of Phe supplementation. All media were supplemented with 10% (v/v) fetal calf serum. After incubation for 6, 30 and 54 h, protein synthesis was measured by the incorporation of L-[2, $6-^{3}H$] Phe into CEF for further 18 h. Protein synthesis of CEF cultured in DMEM was higher than that in PBS ($Ca^{2+}$, $Mg^{2+}$). High specific radioactivity of Phe due to the low concentration of Phe in the medium resulted in the apparent increase in protein synthesis of CEF. Protein synthesis cultured in PBS ($Ca^{2+}$, $Mg^{2+}$) with Phe did not increase during 72 h of cell culture.

• Journal of Plant Biotechnology
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• v.7 no.3
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• pp.175-181
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• 2005

This work described some essential factors necessary for micro-propagation of banana for mass production of synthetic seeds for germ plasm conservation, and how peroxides activity of conserved tissue was influenced. Shoot tips of field grown plants were used to obtain shoot clusters on shoot proliferation medium (MS medium supplemented with 5 mg/l BAP). Using longitudinally-split shoot tip technique, 18720, 8640, 7488, 2016 plantlets were obtained from one shoot tip of Maghraby, Grand Naine, Balady, and Williams, respectively, in six subculture, one month each, on solid medium. Shoot tips excised from in vitro grown plantlets were encapsulated in calcium-alginate beads and stored at $4^{\circ}C$ for one month on half-strength MS basal medium without growth regulators or sugars. After one month all the viable-conserved synseeds formed shoots when they were transferred to MS basal medium, some of them showed synchronous formation of shoot and root systems in one week. Plants retrieved from encapsulated shoot tips were hardened off and transferred to soil.

• Journal of Magnetics
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• v.6 no.4
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• pp.109-118
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• 2001

A computer simulation, utilizing the Landau-Lifshitz-Gilbert equation, of ultra-high- density recording on continuous longitudinal media is carried out. The two important features of this work are the use of a planar-type head, which enables a high write field of 14183 Oe ts be generated at the center of the recording medium, and the media with very high coercivities up to 13010 Oe. From a systematic investigation, it is found that the optimum write field is higher than the medium coercivity by only 3400 Oe over a wide coercivity range. This new finding allows one to write an a medium with a very high coercivity by using a planar-type head. It is demonstrated that a reasonably good bit pattern with a bit density of 605 kfci is generated on the medium with a coercivity of l1720 Oe, and, combined with a high track pitch density of 100 ktpi, a recording density of 60 Gb/in$^2$can be obtained in a single layer medium. With an improved write- head designs even a higher recording density of 75 Gb/in$^2$may be possible since comparison of the results for the bit pattern from the present head profile and the ideal Lindholm profile indicates an increase in the track pitch density of about 27%. Even at this density, the thermal stability parameter (KV/kT) at room temperature is high enough (60) to provide ample room for thermal stability.

• Journal of The Korean Astronomical Society
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• v.38 no.2
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• pp.219-222
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• 2005

We present the high-resolution (2"-4") images of the molecular envelopes surrounding the evolved stars, V Hya, VY CMa, and ${\pi}^1$ Gru observed with the Submillimeter Array. The CO J=2-1 and 3-2 images of the carbon star V Hya show that the circumstellar structure of this star consists of three kinematic components; there is a flattened disk-like envelope that is expanding with a velocity of ${\~}16 km\;s^{-1}$, the second component is the medium-velocity wind having a deprojected velocity of 40-120 km $s^{-l}$ moving along the disk plane, and the third one is the bipolar molecular jet having an extreme velocity of 70-185 km $s^{-l}$. The axis of this high velocity jet is perpendicular to the plane of the disk-like envelope. We found that the circumstellar structure of the S-star ${\pi}^1$ Gru traced by the CO J =2-1 resembles that of V Hya quite closely; the star is surrounded by the expanding disk-like envelope and is driving the medium-velocity wind along the disk plane. We also obtained the excellent images of VY CMa with the CO and $^{13}CO$ J=2-1 and $SO\;6_5-5_4$ lines. The maps of three molecular lines show that the envelope has a significant velocity gradient in the east-west direction, suggesting that the envelope surrounding VY CMa is also flattened and expanding along its radial direction. The high-resolution images obtained with the SMA show that some AGB stars are associated with the asymmetric mass loss including the equatorial wind and bipolar jet.

• Korean Journal of Plant Tissue Culture
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• v.21 no.3
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• pp.157-160
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• 1994

Culture conditions for high Sequency somatic embryogenesis and plant regeneration in tissue cultures of sweet potato of two Korean cultivars 'Puyojaerae' and 'Yulmi' are described. Shoot apical meristem explants (height 150 $\mu\textrm{m}$; base: 350 $\mu\textrm{m}$) were cultured on MS medium supplemented with 1 mg/L 2,4-D. After 6 weeks of culture, greater than 80% of the survived explants produced embryogenic calli. When transferred onto MS medium with 0.1 mg/L each of 2,4-D and kinetin, the calli gave rise to somatic embryos at frequencies of 71% ('Puyojaerae') and 63% ('Yulmi'), respectively: When somatic embryos at various developmental stages measured in length were transversely cut into two halves and cultured on MS medium with 1 mg/L 2,L-D, the upper halves produced secondary embryos more frequently than the lower ones, and halves of somatic embryos less than 1 mm in length had a higher competence for secondary embryo formation than longer ones of either cultivar. However 'Puyojaerae' somatic embryo halves showed a higher frequency of secondary embryo formation than 'Yulmi' ones on the whole. Upon transfer onto MS basal medium, most of the primary and secondary somatic embryos underwent development into plantlets. The plantlets were transplanted to potting soil and grown to maturity in a phytotron. The overall results suggest that the shoot apical meristem culture system for somatic embryo formation in sweet potato previously established by us (SABRAOJ 21: 93-101) may be applicable regardless of it genotypes.

• Korean Journal of Weed Science
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• v.17 no.4
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• pp.390-399
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• 1997

This experiment was conducted to introduce PAT (phosphinothricin acetyltransferase, non-selective herbicide bialaphos resistant gene) gene into potato (Solanum tuberosum. cv. Desiree). Optimal shoot regeneration from leaf discs and stem segments was obtained in MS medium supplemented with 0.1 mg/L IBA and 0.5 mg/L BA, and the frequency of shoot regeneration was 54% in left discs and 46% in stem segments. In this condition, leaf discs and stem segments of potato were co-cultivated with A. tumefaciens MP90 which contained binary vector with GUS: :NPTII gene and PAT gene. Transgenic shoots were regenerated from leaf and stem-derived calli on selection medium with 100mg/L kanamycin. The 100${\mu}M$ acetosyringone treatment during the co-cultivation highly enhanced(4 times than the control) the shoot regeneration on selection medium. When the putative transgenic plants were transferred to medium with 10mg/L basta, all of them were survived. After PCR. GUS test, and Southern blot analysis of the survived plant, we confirmed that the gene was stably integrated into the potato genome and expressed. After the transgenic plants were transplanted in soil, and the transgenic plants were sprayed with the herbicide basta (300ml/10a), the transgenic plants remained green but control plants were died.

• Plant Biotechnology Reports
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• v.3 no.3
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• pp.205-211
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• 2009

An efficient in vitro regeneration protocol for moth bean [Vigna aconitifolia (Jacq.) Marechal] via somatic embryogenesis has been developed. Embryogenic callus cultures were established from the cotyledonary node as explant on semi-solid Murashige and Skoog (MS) medium supplemented with $0.75mg\;1^{-1}$ 2,4-dichlorophenoxyacetic acid (2,4-D) and $1.5mg\;1^{-1}$ 6-benzylaminopurine (BA) and with various additives ($50mg\;1^{-1}$ ascorbic acid and $25mg\;1^{-1}$ each of adenine sulphate, citric acid and $_L-arginine$). Numerous somatic embryos differentiated on MS basal nutrient medium supplemented with $0.25mg\;1^{-1}$ 2,4-D and $0.5mg\;1^{-1}$ of kinetin (Kin). Sustained cell division resulted in the formation of cell aggregates, which progressed to the globular- and heart-shaped somatic embryos and then, if they differentiated properly, to the torpedo shape and cotyledonary stages. The transfer of embryos onto fresh MS basal medium containing $0.2mg\;1^{-1}$ BA and $2.0mg\;1^{-1}$ gibberellic acid enabled the embryos to achieve complete maturation and germination. More than 80% of somatic embryos were converted into true-to-type fertile plants. In vitro-regenerated plantlets with well-developed roots were successfully hardened in a greenhouse and established in soil.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.2
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• pp.164-167
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• 2004

This study was carried out to investigate in vitro fertilization and development of in vitro matured pig oocytes inseminated with the Duroc boar sperm by different sperm washing media after thawing of the 5 ml frozen straws. Immature follicular oocytes (30-40) were transferred into each well of a Nunc 4-well multidish containing $500{\mu}l$ mTCM199 maturation medium. The sperm rich portion of ejaculates was collected into a 250 ml insulated vacuum bottle and gradually cooled 22 to $24^{\circ}C$ over a 2 h period. Semen was centrifuged at 800 g for 10 min and the seminal plasma discarded. Sperm were esuspended in a lactose-egg yolk and N-acetyl-Dglucosamine (LEN) diluent to contain $1{\times}10^{9}$ sperm/ml and cooled to $5^{\circ}C$ over a 2 h period. Immediately before freezing, semen was rediluted with an equal volume of LEN+4% glycerol and packed into 5 ml straws. After thawing of the 5 ml straw, the 5 ml semen was diluted with 20 ml Beltsville thawing solution (BTS) at room temperature. Oocytes were inseminated with untreated (unwashed and nonpreincubated) or treated sperm (washed two times in BTS, mTLP-PVA and mTBM media, respectively and nonpreincubated) with $2{\times}10^{7}$ sperm concentration. Oocytes were coincubated for 6 h in $500{\mu}l$ mTBM fertilization. At 6 h after IVF, oocytes were transferred into $500{\mu}l$ NCSU-23 culture medium for further culture of 6 h. Sperm penetration, polyspermy and male pronuclear formation of oocytes at 12 h after IVF and developmental ability of oocytes at 48 h after IVF were evaluated. Sperm penetration rate, male pronuclear formation and rate of cleaved embryos were higher in the BTS, mTLP-PVA and mTBM treatments than the unwashed treatment (p<0.05). The rate of blastocysts from the cleaved oocytes (2-4 cell stage) were higher in the mTLP-PVA treatment than in the unwashed, BTS and mTBM treatments. In conclusion, we recommend the washing of frozen-thawed sperm with mTLP-PVA medium before in vitro fertilization of oocytes in mTBM medium.

• Journal of Bio-Environment Control
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• v.23 no.3
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• pp.235-243
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• 2014

This research was conducted to investigate the influence of leaching fractions (LF) in each irrigation or fertigation on plant growth and changes in chemical properties of root media during the production of seedling grafts of tomato. Two root media containing Sphagnum peat moss plus vermiculite (5:5, v/v, PV) and coir dust plus vermiculite (5:5, v/v, CV) were formulated and pre-planting fertilizers were incorporated during formulation. Then, each medium was packed into 50 cell (volume 33 cc) and 105 cell (volume 18 cc) trays and the rootstock (cv. J3B Strong) and scion (cv. Sunmyung) were grown, respectively. The seedlings were grafted at 31 days after sowing and then the cut seedling grafts (Sunmyung scion/J3B Strong rootstock) were planted into 50 cell plug trays containing each of the two root media. After induction of the graft union and new adventitious roots for 7 days, the seedling grafts were fed with fertilizer solution once a week containing 4 different N concentrations (0, 50, 100, $200mg{\cdot}L^{-1}$). When determined after 31 days from seed sowing, the highest fresh weights of the root stock seedlings were obtained with 0.75 LF in PV (8.96g/seedling) and CV (7.11g/seedling) mixes. The EC of the both mixes were 0.93 and $1.09dS{\cdot}m^{-1}$, respectively. The fresh weights of the scion seedlings 31 days after seed sowing were 4.29g with 0.50 LF in the PV and 3.13g with 0.50 LF in the CV. The root medium ECs of the two treatments were 0.76 and $1.34dS{\cdot}m^{-1}$, respectively. Fresh weights of the seedling grafts grown for 31 days were greatly influenced by post-planting fertilizer concentrations. The heavier plants were obtained in $100mg{\cdot}L^{-1}$ N treatment than any other treatments in same mixes. The substrate ECs in these two treatments were 0.98 and $1.93dS{\cdot}m^{-1}$, respectively, indicating that the desirable range of soluble salts in soil extracts is higher in the CV mix than the PV mix. Results of this study suggest that optimum EC range is different in each medium and LF need to be adjusted differently for each root medium to produce high quality seedling grafts of tomato.