• 제목/요약/키워드: L-ARS

검색결과 73건 처리시간 0.027초

L-ARS를 이용한 Cl II div. 1 부정교합의 치험례 (TREATMENT OF CLASS II DIVISION 1 MALOCCLUSION WITH L-ARS(LIGATED ANTERIOR REPOSITIONING SPLINT))

  • 김종철
    • 대한치과교정학회지
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    • 제23권4호
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    • pp.447-454
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    • 1993
  • Patient with skeletal class II relationship was treated with L-ARS. L-ARS is fixed functional appliance that could be effective in children and adolescent patient who don't wear activator. The following results were obtained ; 1) Growth of Mandible was stimulated and overjet was decreased, therefore coupling of anterior teeth was established with L-ARS which is fixed functional appliance. 2) These changes were accomplished with Mandibular skeletal growth with no effect on the Maxilla. 3) L-ARS was especially effective on patient who deny to wear the removable functional appliance.

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Methionine Analogue Probes Functionally Important Residues in Active Site of Methionyl-tRNA Synthetase

  • Jo, Yeong-Joon;Lee, Sang-Won;Jo, Myung-Kyun;Lee, Jee-Woo;Kang, Mee-Kyoung;Yoon, Jeong-Hyeok;Kim, Sung-Hoon
    • BMB Reports
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    • 제32권6호
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    • pp.547-553
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    • 1999
  • Aminoacyl-tRNA synthetases are essential enzymes catalyzing the attachment of specific amino acids to cognate tRNAs. In the present work, the substrate analogue L-methionine hydroxamate was used to identify functional residues located in the active site of the E. coli methionyl-tRNA synthetase (MetRS). This compound inhibited bacteria, yeast, and human MetRS activities to a similar degree, suggesting a conserved active site structure and mechanism between MetRSs of different phylogenetic domains. Mutants of the E. coli MetRS resistant to methionine hydroxamate were also isolated. These mutants contained a substitution either at T10, Y15, or Y94. These residues are highly conserved among the different MetRSs and the mutants showed decreased aminoacylation activity, suggesting their functional and structural significances. The putative roles of these residues are discussed on a structural basis.

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Development and Validation of a Predictive Model for Listeria monocytogenes Scott A as a Function of Temperature, pH, and Commercial Mixture of Potassium Lactate and Sodium Diacetate

  • Abou-Zeid, Khaled A.;Oscar, Thomas P.;Schwarz, Jurgen G.;Hashem, Fawzy M.;Whiting, Richard C.;Yoon, Kisun
    • Journal of Microbiology and Biotechnology
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    • 제19권7호
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    • pp.718-726
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    • 2009
  • The objective of this study was to develop and validate secondary models that can predict growth parameters of L. monocytogenes Scott A as a function of concentrations (0-3%) of a commercial potassium lactate (PL) and sodium diacetate (SDA) mixture, pH (5.5-7.0), and temperature (4-37DC). A total of 120 growth curves were fitted to the Baranyi primary model that directly estimates lag time (LT) and specific growth rate (SGR). The effects of the variables on L. monocytogenes Scott A growth kinetics were modeled by response surface analysis using quadratic and cubic polynomial models of the natural logarithm transformation of both LT and SGR. Model performance was evaluated with dependent data and independent data using the prediction bias ($B_f$) and accuracy factors ($A_f$) as well as the acceptable prediction zone method [percentage of relative errors (%RE)]. Comparison of predicted versus observed values of SGR indicated that the cubic model fits better than the quadratic model, particularly at 4 and $10^{\circ}C$. The $B_f$and $A_f$for independent SGR were 1.00 and 1.08 for the cubic model and 1.08 and 1.16 for the quadratic model, respectively. For cubic and quadratic models, the %REs for the independent SGR data were 92.6 and 85.7, respectively. Both quadratic and cubic polynomial models for SGR and LT provided acceptable predictions of L. monocytogenes Scott A growth in the matrix of conditions described in the present study. Model performance can be more accurately evaluated with $B_f$and $A_f$and % RE together.

적응 알고리즘을 이용한 ESC와 ARS 기반 요 모멘트 분배 (Adaptive Algorithms for Yaw Moment Distribution with ESC and ARS)

  • 임성진
    • 대한기계학회논문집A
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    • 제40권12호
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    • pp.997-1003
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    • 2016
  • 본 논문은 자세 제어 장치와 능동 후륜 조향장치를 가지는 통합 섀시 제어에서 요 모멘트 분배를 위해 적응 알고리즘을 적용하는 방법을 제안한다. 통합 섀시 제어는 상위제어기와 하위제어기로 구성된다. 상위제어기에서 슬라이딩 모드 제어 이론을 이용하여 차량을 안정화시키는데 필요한 제어 요 모멘트를 계산한다. 하위제어기에서는 제어 요 모멘트를 만들어 내기 위해 자세 제어 장치의 제동 압력과 능동 후륜 조향장치의 조향각을 결정하는 데에 적응 알고리즘을 적용한다. 차량 시뮬레이션 패키지인 CarSim에서 시뮬레이션을 수행하여 제안된 방법의 타당성을 검증한다.

미꾸라지로부터의 복제원점 클로닝 및 그 특성에 관한 연구 (Cloning and Characterization of Replication Origins from Misgurnus mizolepis)

  • 임학섭;김무상;이형호
    • 한국양식학회지
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    • 제8권3호
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    • pp.209-220
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    • 1995
  • 미꾸라지의 간으로부터 핵을 분리하여, 저농도 염추출 및 제한효소 처리로 핵기질(nuclear matrix)을 분리하였다. 분리된 핵기질을 Proteinase K로 분해한 후, phenol-chloroform 추출로 크기가 약 0.3kb-15kb의 분포를 나타내는 핵기질 부착 DNA (nuclear matrix attachment regions : MARs)를 얻었다. 효모 URA 3 유전자를 가진 2.13 kb Eco47 III 단편을 제한효소 Ssp I 으로 절단된 pUC19 플라즈미드 벡타에 결합시켜, ARS (autonomously replication sequence) 클로닝을 위한 pURY19 플라즈미드 벡타를 만들었다. 이 pURY19 벡타는 Saccharomyces cerevisiae내에서 독립적으로 복제할 수 없기 때문에, 물고기의 효율적인 발현 벡타 개발을 위해, 이 system을 이용하여, S. cerevisiae내에서 독립적으로 복제 가능한 미꾸라지의 ARS를 클로닝하고자 하였다. 분리 된 MARs를 pURY19 벡타에 결합시 킨 다음, E. coli $DH5\alpha$에 형질전환시켜 $pURY19N_{l-62}$를 얻었다. MAR Libraries $(pURY19N_{1-62})$를 각각 $Ura^-\;S.\;cerevisiae$에 형질전환시켜, S. cerevisiae내에서 독립적으로 복제 가능한 M. mizolepis 유래의 복제원점들 (ARSs)을 분리하여, Sanger's dideoxy-chain termination method로 염기서열을 분석하였다. 염기서열 분석결과 모든 clones들은 AT-rich하였으며, 특히 $pURY19N_6$에는 ARS concensus sequence, Topoisomerase II consensus, near A-box, 그리고 T-box들이 존재하였다.

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Empirical Relations of Nutrients, N : P Ratios, and Chlorophyll in the Drinking Water Supplying Dam and Agricultural Reservoirs

  • Lee, Sang-Jae;An, Kwang-Guk
    • 생태와환경
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    • 제41권4호
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    • pp.512-518
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    • 2008
  • This study were to evaluate trophic conditions, N : P ratios, and empirical relations of chlorophyll (CHL) systematically using TN, TP, and CHL values in agricultural reservoirs and drinking water supplying dams. During the study, nutrients and CHL varied depending on seasonal conditions and types of the reservoirs, but most reservoirs were diagnozed as eutrophic to hypertrophic. Mass ratios of TN : TP averaged 93.1 (range: $0.68{\sim}1342$) and about 96.6 % of the total observations (n=516) was > 17 in the N : P ratios. This result suggests that P was a potential factor limiting algal growth in the entire reservoir. Thus, TN : TP ratios were a function of phosphorus rather than nitrogen. Regression analysis of log-transformed N : P ratios against TP in DWDRs and ARs showed that ratios were linearly declined with an increase of TP ($R^2$>0.66; p<0.001). Seasonal mean CHL was minimum ($4.3{\mu}g\;L^{-1}$, range: $0.1{\sim}39.7{\mu}g\;L^{-1}$) in premonsoon, and was similar between the monsoon and postmonsoon. In contrast, one of the tremendous features was that values of CHL was greater in the ARs than DWDRs. Thus, the spatial and temporal patterns in CHL were similar to those of TP but not TN. Empirical models of CHL-TP showed that CHL variation could explain average 15.3% and 11.3% in DWDRs and ARs, respectively. Seasonal analysis of empirical models showed that CHL-TP relations were stronger in postmonsoon than those of premonsoon and monsoon.

Comparison of different colorimetric assays and application of the optimized method for determining the liberated fluoride contents in various tea extracts

  • Le-Thi Anh-Dao;Do Minh-Huy;Nguyen-Ho Thien-Trang;Nguyen Cong-Hau
    • 분석과학
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    • 제37권2호
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    • pp.87-97
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    • 2024
  • The appropriate intake of fluoride (F-) is beneficial to human health; however, the over-consumption can result in various potentially harmful effects. This study compared different colorimetric reagents, i.e., aluminium-xylenol orange (Al-XO), zirconium-xylenol orange (Zr-XO), and zirconium-alizarin red S (Zr-ARS), for fluoride measurements by the UV-Vis, in terms of reaction mechanisms, method sensitivity, and interferences from aluminium and ferric ions. The colorimetric procedures were optimized, and the analytical methods were evaluated. The goodness of linearity (R2 > 0.998) was obtained for all three assays within the concentration range of 1.0-20.0 mg/L fluoride in deionized water, in which the method sensitivity followed the descending order of Zr-XO > Al-XO > Zr-ARS. The Zr-XO was applied for determining the fluoride in different tea extracts in water (90 ℃ and 60-minute-brewing) and black tea demonstrated the highest fluoride content (3.0-3.6 mg/L). The effects of brewing time and temperature on the release of fluoride in the tea extracts were also investigated, indicating these are critical factors for the fluoride extraction. This study highlighted the application potentials of the UV-Vis measurement as a simple, convenient, and cheap analytical approach and discussed different colorimetric reagents used for fluoride determination in tea extracts in the context that the UV-Vis spectrophotometers are commonly equipped in most laboratories.