• 시스템엔지니어링학술지
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• 제12권1호
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• pp.59-71
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• 2016

The Nuclear and Fossil Steam Turbines record a considerable number of failures annually. Some of these failures reported are as result of blade failure. The failure of the L-0 blade in a Steam Turbine is one of the most reported blade failure in Nuclear and Fossil steam turbines. This paper seeks to identify the best Non Destructive Evaluation (NDE) method or methods to be used in the steam turbine L-0 blades inspection process. The development of systems engineering processes presents an opportunity to apply NDE inspection to the L-0 blades. This process apply computer modelling of the L-0 using ANSYS and by simulating the stresses experienced by the L-0 blade during operation it is possible to identify the most susceptible areas for crack formation and growth. The results from these models compared to industry data for validation. The analysis of these results used to predict the most probable failure location and failure modes. Therefore NDE inspection can be applied to these areas with greater degree of accuracy. This would be beneficial in the increasing the accuracy in the detection of cracks and hence save inspection time and the overall inspection cost. Furthermore, not only the location for crack formation and NDE inspection determined but also best the NDE inspection technique/techniques to be applied appropriately on the L-0 blade are prescribed.

• Plant Resources
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• 제4권1호
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• pp.53-58
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• 2001

The effect of carbenicillin on the dedifferentiation and the regeneration efficiency of plant tissues of horseradish(Armoracia rusticana) was evaluated, Inhibition effect for callus initiation was observed when leaf blade, root and petiole segments were grown on MS medium containing 500 mg/L to 2000 mg/L carbenicillin and 0.5 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D). The regeneration of horseradish shoots from leaf blade, root and petiole explants were decreased as the addition of carbenicillin increased from 1000 mg/L to 2000 mg/L in MS medium containing 0.5 mg/L of 6-benzylaminopurine (BAP) or kinetin. Especially, 500 mg/L carbenicillin treatment significantly inhibited shoot induction when leaf blade explants were grown on hormone-free MS medium. It was suggested that the toxic effects of combinations of carbenicillin and 2,4-D may be due to high auxin activity levels.

• 대한기계학회논문집B
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• 제37권2호
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• pp.149-154
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• 2013

본 연구는 7kW 모터로 $18.5m^3/min$의 유량을 양정(H) 0.5m로 공급할 수 있는 고농도 폐수처리용 3엽 수중 펌프의 블레이드를 개발하는 것을 목표로 한다. 이를 위해 블레이드의 축방향 비틀림 각, 블레이드의 길이 및 반경방향 비틀림 각을 설계변수로 선정하여 이들이 블레이드 효율에 미치는 영향을 상용 해석용 프로그램을 사용한 (ANSYS BladeGen, Turbo Grid, CFX) 전산해석을 통해 수행하였다. 해석 결과 블레이드의 축방향 비틀림각(${\beta}$)가 펌프의 효율에 가장 민감한 변수임을 알 수 있었으며, 축방향 비틀림각 $({\beta})=20^{\circ}$, 반경방향 비틀림 각 $({\alpha})=110^{\circ}$ 그리고 블레이드의 길이 (l)=240 mm 일 때 펌프의 최고 효율을 가지게 됨을 알 수 있었다.

• 식물조직배양학회지
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• 제26권4호
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• pp.245-250
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• 1999

수분 후 담배의 배에 중이온 ($^{14}$ N) beam을 조사하여 유도한 variegated담배의 잎 조직을 기내배양하여 재생된 식물체의 특성을 조사하였다. NAA 0.1mg/L 와 BAP 1/0mg/L를 첨가한 MS배지에서 variegated담배의 잎을 배양하면 백색부위에서는 백색 식물체만이 유도되었으나 녹색부위에서는 녹색 식물체가 47.2%, 백색 식물체가 37.4%, variegated식물체가 15.4% 유도되었다. 재생된 녹색 식물체는 자가수분한 후대 (F$_1$)에서 녹색 식물체 1,651 개:백색 식물체 54개로 분리되었다. 또한 variegated 잎에서 재생된 녹색 식물체와 정상주를 정역교배 한 결과, 전부 녹색체가 분리되었다. 이 결과는 variegated잎의 표현형 유전은 최소한 모성 유전이 아님을 보여준다. Variegated잎의 녹색부위를 배양하여 재생된 variegated식물체의 잎을 이용하여 DNA gel blot한 결과, 엽록체 유전자인 psbA, rbcL, 165 rDNA, 23S rDNA의 양은 흰색부위 잎에서 정상주인 녹색 잎과 variegated식물체 잎의 녹색부위 보다 많았다.

• 한국수산과학회지
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• 제42권4호
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• pp.360-365
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• 2009

The dietary fiber and crude fiber contents of different thallus regions (blade, stipe, and holdfast) in three brown algae (Laminaria japonica, Ecklonia stolonifera, and E. cava) were detennined at different ages, and then compared with one another. On a dry matter basis, the soluble dietary fiber (SDF) content was highest ($10.8{\pm}0.5%$) in the holdfast of 2-year old L. japonica, and the insoluble dietary fiber (IDF), total dietary fiber (TDF), and crude fiber (CF) contents were highest in the holdfast of 2-year old E. cava at $44.5{\pm}0.7%$, $50.2{\pm}0.5%$, $6.8{\pm}0.7%$, respectively. The IDF, TDF, and CF contents of these three species were measured in the following order: holdfast > stipe > blade, and the SDF contents of L. japonica exhibited the reverse of this trend. The TDF/CF ratio of 1-year old L. japonica, E. stolonifera, and E. cava was greater than was observed in the corresponding 2-year old samples. This is, to the best of our knowledge, the first report demonstrating that the holdfasts of L. japonica, E. stolonifera, and E. cava are rich in dietary fiber contents, especially IDF, TDF, and CF.

• International Journal of Aeronautical and Space Sciences
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• 제7권1호
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• pp.54-64
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• 2006

To augment weakly damped lag mode stability of a hingeless helicopter rotor blade in hover, piezoelectric shunt with a resistor and an inductor circuits for passive damping has been studied. A shunted piezoceramics bonded to a flexure of rotor blade converts mechanical strain energy to electrical charge energy which is dissipated through the resistor in the R-L series shunt circuit. Because the fundamental lag mode frequency of a soft-in-plane hingeless helicopter rotor blade is generally about 0.7/rev, the design frequency of the blade system with flexure sets to be so. Experimentally, the measured lag mode frequency is 0.7227/rev under the short circuit condition. Therefore the suppression mode of this passive damping vibration absorber is adjusted to 0.7227/rev. As a result of damping enhancement using passive control, the passive damper which consists of a piezoelectric material and shunt circuits has a stabilizing effect on inherently weakly damped lag mode of the rotor blades, at the optimum tuning and resistor condition.

• ALGAE
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• 제20권2호
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• pp.151-155
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• 2005

To obtain basic data, we investigated the effect of blade length on transplants, the transplanting method of Laminaria japonica for creating L. japonica resources and the number of transplanting plates with surviving L. japonica. The survival rate of L. japonica, blade length of transplants and drag force of transplanting plates were also researched. The number of transplanting plates with surviving L. japonica, the survival rate and blade length of 20 cm long-initial transplants were greater than those of 1.5, 5 and 10 cm long-initial transplants in an outdoor aquarium. At the depth of 4 m in the coastal waters, the number of transplanting plates with surviving transplants, the survival rate and the blade length of 30 cm long-initial transplants were higher than those of 10 and 20 cm longinitial transplants. The drag force is calculated by cording up sporophytes of L. japonica into the transplanting plates under water. The drag force in the case of a 2.18 kg-weight transplanting plate and in a current speed of 0.5 m${\cdot}s^{-1}$ for considering stability of the plate was 631.50 g to a concrete substratum on the seabed, 703.92 g to a shingle substratum, 788.00 g to a sand substratum, and 1018.30 g to a silt substratum. If we consider the stability and economic efficiency of the transplanting plate, the proper weight of the plate per one individual of 18.11 cm in blade width and 190.20 cm in total blade length is regarded as 508.2 g when it is calculated with the concrete substratum that shows the lowest drag force.

• 식물조직배양학회지
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• 제21권4호
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• pp.247-252
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• 1994

조직배양을 통한 대량증식을 목적으로 구문초 (Pelalgonium citrosa)의 엽신, 엽병 및 정단분열조직을 auxin류와 cytokinin류의 생장조절제를 혼합첨가한 MS배지에 배양한 후 캘러스발생 및 shoot분화율을 조사하였고, 분화된 shoot는 NAA와 IAA가 단독첨가된 MS배지에 옮겨 뿌리분화율을 조사하였다. 생장조절제의 효과는 2,4-D와 kinetin의 혼용처리보다 NAA와 BA첨가배지에서 엽신, 엽병조직 모두 캘러스발생 및 multiple shoot분화가 양호하였다. 엽신조직의 경우 암배양 상태에서 캘러스와 shoot의 분화가 좋았고, 0.5 mg/L NAA와 BA 혼용배지에서는 정상적 민 shoot가 분화된 반면 NAA와 BA의 농도가 높을 경우 분화된 shoot는 vitrification현상이 비교적 많이 나타난다. Shoot는 캘러스를 통하거나 직접 절편체에서 분화되었는데 1개의 절편당 shoot분화수는 캘러스를 통해 분화된 경우가 훨씬 많았다. 엽보의 경우 캘러스발생 및 shoot분화양상은 엽신과 비슷하였으나 분화된 shoot수는 훨씬 적었다. 정단분열조직에서 캘러스증식율이 가장 좋았으며 shoot분화는 NAA와 BA 0.5mg/L첨가구에서 양호하여 대량증식에 가장 효과적인 반응이었다. Shoot로부터 뿌리발생은 1.0 mg/L NAA 첨가배지에서 가장 효과적이었다.

• Journal of Plant Biotechnology
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• 제1권2호
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• pp.101-107
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• 1999

The leaf blade-segments of albino tobacco (Nicotiana tabacum L.) were cultured on MS media containing different concentrations of BAP (0, 0.4, 2.2, 4.4, 22.2 ${\mu}{\textrm}{m}$) with or without NAA (0, 0.5, 2.7 ${\mu}{\textrm}{m}$). Multiple shoots were induced on the media containing 0.4 to 2.2 ${\mu}{\textrm}{m}$ BAP. The best condition for multiple shoot induction with root formation was MS media containing 4.4 ${\mu}{\textrm}{m}$ BAP and 0.5 ${\mu}{\textrm}{m}$ NAA. The regenerated albino plants showed a significant reduction in accumulation of chlorophylls and carotenoids. The drastic reduction of the pigments content was associated with the distinct alterations in gene expression in the albino plants. firstly, the expression of plastid genes, such as rbcL, psbA, 165 rDNA and 235 rDNA, was reduced at the level of transcripts in the regenerated albino plants. Secondly, the alteration of structure of the plastid genes was not detected in the albino plants. However, the copy number of the plastid genes whose transcription level was reduced greatly was increased approximately two-fold, although the transcriptions of nuclear gene (255 rDNA) showed the wild-type level.

• 동력기계공학회지
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• 제11권1호
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• pp.5-15
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• 2007

In the recently released accident-investigation report on blade failure, almost 70% of blade failures was found at low pressure turbine blades, and it is well known that main cause is due to the vibration modes. This paper describes the systematic approach on the root cause of the blade failure at L 0 stage, 30MW single flow industrial steam turbine which had tripped by high vibration after ten-month commercial operation. A fracture was found at the only one damping wire hole of 59 blades, and crack was detected at three damping wire holes by NDT. According to the analysis result for the crack fracture surface and the chain of the sequential operational events, we come to the conclusion that a typical high cycle fatigue is the most dominant factor caused to the blade failure, the resonance frequency margin was narrowed by the cut damping wire and the high cycle vibration was amplified, and then the blade was broken at once by the centrifugal force when the crack reached the critical size.