• Title/Summary/Keyword: L-

Search Result 82,303, Processing Time 0.095 seconds

Studies on the Virus in Silkworm Bombyx mori L. -Resistance to Virus Disease- (가잠 Virus에 관한 연구 -저항성에 관한 기초조사-)

  • 박광의;강석권
    • Journal of Sericultural and Entomological Science
    • /
    • v.9
    • /
    • pp.67-87
    • /
    • 1969
  • 1. Objectives and Importance. Many silkworms have been damaged by nuclear polyhedrosis virus diseases thoughout the country every year causing a decease in cocoon production by approximately ten per cent per year. The damage caused by the infections virus has occured in spite of complete disinfection. In this respect, it is well known it is impossible, at the present time, to protect the silkworm from these virus infections through chemical and physical control methods. Therefore, this author has attempted to solve this urgent problem from the view point of heredity and breeding, discovering the different resistances and heritabilities among 120 stains collected from throughout the country, and selecting the ones with highest resistance for the basic materials in the silkworm breeding. 2. Results of work 1) The strains with strong resistance to the nuclear polyhedrosis virus diseases are N$_4$, N$\sub$15/, N$\sub$48/, C$\sub$55/ and Em. the log ED$\sub$50/ values of them vary between 0.799 and 1.611. The susceptible strains are N$\sub$20/, C$\sub$62/, N$\sub$76/, N$\sub$79/ and C$\sub$108/, the log ED$\sub$50/ values of them vary between 5.159 and 7.258. (Reference Table 4) The Japanese strain with a log ED$\sub$50/, value of 3.770 is the strongest, followed by the Chinese strain with a log ED$\sub$50/ value of 3.564. The weakest is the European strain with a log ED$\sub$50/ value of 3.3381. The direction coefficient of the regression equation of the susceptibility varies between 0.1 and 0.6, the uniformness of the resistance of the preserved strains of this country is comparatively low. The hereditary henomena of the resistance of each strain and the conerete method of its application for silkworm breeding main the subjects for later studies. 2) The content of water and ash in silkworm has not been correlated with the capability for resistance to the virus diseases(Reference. Table 8). but it is very significantly correlated with mortality rate (in common reaning). In the case of the silkworms which have just completed the fourth moulting the content of water and ash is not related to the mortality rate. In the case of the silkworms which have just completed the third moulting, however, the water( +0.326) and ash (+0.362) registered a high significance. The ash content in the first ($\div$0.520) and second ($\div$0.386) moults is highly significant but water content in both cases is not significant (Reference Table 7). 3) The No. 205 strain proved to be the best in character among the various F$_1$ hybrids. No. 204 was very good in strength but a little lower in cocoon character than the control. No. 212 was a little low in coccon character and mortality was average, but the cocoon harvest was the best among all the varieties offered for (Reference Table 9). 4), In short, the above mentioned strains which are known to have strong resistance to the virus disease are expected to provide basic data for breeding strong varieties. It is proposed that continued research should be conducted on the characteristics of various strains for a satisfactory preservation of various characteristics.research should be conducted on the characteristics of various strains for a satisfactory preservation of various characteristics.

  • PDF

Studies on Control of the Mixture of Annual and Perennial Weeds Emerged from Paddy Field - On the Pre-Emergence Treatment of Herbicides in the Paddy Field Dominated by Sagittaria pygmaea MIQ - (다년생잡초(多年生雜草) 혼생답(混生畓)에 있어서 제초제(除草劑)에 의한 잡초방제(雜草防除) - 특(特)히 올미 우점답(優點畓)에서 초기처리제(初期處理劑)를 중심(中心)으로 -)

  • Ryang, H.S.;Han, S.S.;Kim, J.S.
    • Korean Journal of Weed Science
    • /
    • v.2 no.1
    • /
    • pp.31-40
    • /
    • 1982
  • For the effective control of weeds in mechanically transplanted paddy field weeding effects of naproanide ${\alpha}$-(${\beta}$-naphthoxy) propion anilide], pyrazolate [4-2, 4-dichlorobenzoyl)-1, 3-dimethyl pyrazol-5-yl-p-tolune sulphanate], chlormethoxynil (2, 4-dichloro-phenyl-4-nitro-3-methoxy phenyl ether), SL-49 [1-3dimethyl-4(2, 4dichlorobenzoyl)-5-phenacyloxy pyrazole], ACN (3-chloro-2-amino-l, 4-naphthoquinone) either alone or in combination with butachlor (2-chlor-2, 6-diethyl-N-buthoxymethyl acetanilide) were compared. Pyrazolate and SL-49 were most effective for the control of Sagittaria pygmaea MIQ. and Potomogeton distinctus A. BENN. including most annual weeds. Weeding effect of butachlor alone was very high for annuals, good for Cyperus serotinus ROTTB. and poor for S. pygmaea and P. distinctus. But the weeding effect of the combination of butachlor and pyrazolate was stronger than that of butachlor alone and therefore this mixture was effective for S. pygmaea, P. distinctus and C. serotinus including all the annual weeds. The combination of butachlor and SL-49 showed the same tendency as the combination of butachlor and pyrazolate. Naproanilide was not effective for the control of Echinochlor crusgalli P. BEAUV and less effective for Monochoria vaginalis PRESL, but excellent for S. pygmaea. By mixing butachlor with naproanilide weeding, spectrum for annuals and S. pygmaea was much increased by that for P. distinctus and C. serotinus was not satisfactory. ACN was not satisfactory for the control of all the tested weeds but the weeding effect was increased in general by mixing with butachlor. Chlormethoxynil was excellent for the control of annual weeds but it has no effect on C. serotinus, S. pygmaea and P. distinctus showing some initial controling effect but these weeds regrew afterwards. The weeding activity of ACN increased in combination with butachlor and the residual activity was stronger than that of ACN alone. A light crop injury was found at the initial period after treatments in all treated plots. The yield from all treated plots except those from plots treated with ACN, butachlor and naproanilide were not significantly different from the band weeded plot.

  • PDF

Antagonistic Interaction between Quinclorac and Bensulfuron-methyl on Growth of the Rice Plants (Quinclorac과 Bensulfuron-methyl의 혼합처리(混合處理)에서 벼의 생장(生長)에 대한 제초제간(除草劑間) 길항작용(拮抗作用))

  • Kwon, Oh-Yeon;Kwon, Yong-Woong
    • Korean Journal of Weed Science
    • /
    • v.17 no.3
    • /
    • pp.288-294
    • /
    • 1997
  • Field and pot expeiments were carried out to evaluate the interaction between quinclorac and bensulfuron-methyl on growth of the rice plants(Oryza sativa L. cv. Choocheongbyeo) at 20, 45, 65 days-old stages. Quinclorac and bensulfuron-methyl showed antagonistic interaction at both stages, which were detected by the Chisaka's method at isobles of 10% growth inhibition. The antagonism indices were -0.63 and -1.67 at 20 and 65 days-old seedling stages, respectively. Leaf-rolling of rice occurred when quinclorac was applied at 600g ai/ha or more at 20 days-old seedling stage, while it occured at the dose of 900g ai/ha at 65 days-old stage. Bensulfuron-methyl reduced plant height and dry weight as well as tiller production at both stages. Leaf-rolling of rice was reduced when mixture of quinclorac and bensulfuron-methyl was applied due to antagonism of the two herbicides. High temperatures increased the phytotoxicity of bensulfuron-methyl, while the phytotoxicity caused by quinclorac alone was not responsive to temperature. The antagonistic effect between quinclorac and bensulfuron-methyl increased at low temperature as tested by the Colby's method.

  • PDF

Studies on the Properties of Populus Grown in Korea (포플러재(材)의 재질(材質)에 관(關)한 시험(試驗))

  • Jo, Jae-Myeong;Kang, Sun-Goo;Lee, Yong-Dae;Jung, Hee-Suk;Ahn, Jung-Mo;Shim, Chong-Supp
    • Journal of the Korean Wood Science and Technology
    • /
    • v.10 no.3
    • /
    • pp.68-87
    • /
    • 1982
  • In Korea, this is the situation at moment that the total demand of timber in 1972 is more than 5 million cubic meters. On the other hand, however, the available domestic supply of timber at the same year is only about, 1 million cubic meters. A great unbalancing between demand and supply of timber has been prevailing. To solve this hard problem, it has been necessitiated to build up the forest stocks as early as possible with fast grown species such as poplar. Under circumstances, poplar plantations which have been carryed on government and private have reached to large area of 116,603 hectors from 1962 up to date. It has now be come a principal timber resources in this country, and required the basic study on various properties of wood for it's proper utilization, since it has not been made of any systematic study on the properties of Populus grown in Korea. In order to investigate the properties such as anatomical, physical and mechanical properties of nine different species (P. euramericana Guiner I-214. P. euramericana Guiner I-476, P. deltoides Marsh, P. nigra var. italica (Muchk) Koeme, P. alba L.,P. alba $\times$ glandulosa P. maximowiczii Henry, P. koreana Rehder, P. davidiana Dode) of poplar for their proper use and development of new ways of grading processing and quality improving, this study has been made by the Forest Research Institute.

  • PDF

Steroid Effect on the Brain Protection During OPen Heart Surgery Using Hypothermic Circulatory Arrest in the Rabbit Cardiopulmonary bypass Model (저체온순환정지법을 이용한 개심술시 스테로이드의 뇌보호 효과 - 토끼를 이용한 심폐바이패스 실험모델에서 -)

  • Kim, Won-Gon;Lim, Cheong;Moon, Hyun-Jong;Chun, Eui-Kyung;Chi, Je-Geun;Won, Tae-Hee;Lee, Young-Tak;Chee, Hyun-Keun;Kim, Jun-Woo
    • Journal of Chest Surgery
    • /
    • v.30 no.5
    • /
    • pp.471-478
    • /
    • 1997
  • Introduction: The use of rabbits as a cardiopulmonary bypass(CPB) animal model is extremely dif%cult mainly due to technical problems. On the other hand, deep hypothermic circulatory arrest(CA) is used to facilitate surgical repair in a variety of cardiac diseases. Although steroids are generally known to be effective in the treatment of cerebral edema, the protective effects of steroids on the brain during CA are not conclusively established. Objectives of this study are twofold: the establishment of CPB technique in rabbits and the evaluation of preventive effect of steroid on the development of brain edema during CA. Material '||'&'||' Methods: Fifteen New Zealan white rabbits(average body weight 3.5kg) were divided into three experimental groups; control CA group(n=5), CA with Trendelenberg position group(n=5), and CA with Trendelenberg position + steroid(methylprednisolone 30 mglkg) administration group(n=5). After anesthetic induction and tracheostomy, a median sternotomy was performed. An aortic cannula(3.3mm) and a venous ncannula(14 Fr) were inserted, respectively in the ascending aorta and the right atrium. The CPB circuit consisted of a roller pump and a bubble oxygenator. Priming volume of the circuit was approximately 450m1 with 120" 150ml of blood. CPB was initiated at a flow rate of 80~85ml/kg/min, Ten min after the start of CPB, CA was established with duration of 40min at $20^{\circ}C$ of rectal temperature. After CA, CPB was restarted with 20min period of rewarming. Ten min after weaning, the animal was sacrif;cod. One-to-2g portions of the following tissues were rapidly d:ssected and water contents were examined and compared among gr ups: brain, cervical spinal cord, kidney, duodenum, lung, heart, liver, spleen, pancreas. stomach. Statistical significances were analyzed by Kruskal-Wallis nonparametric test. Results: CPB with CA was successfully performed in all cases. Flow rate of 60-100 mlfkgfmin was able to be maintained throughout CPB. During CPB, no significant metabolic acidosis was detected and aortic pressure ranged between 35-55 mmHg. After weaning from CPB, all hearts resumed normal beating spontaneously. There were no statistically significant differences in the water contents of tissues including brain among the three experimental groups. Conclusion: These results indicate (1) CPB can be reliably administered in rabbits if proper technique is used, (2) the effect of steroid on the protection of brain edema related to Trendelenburg position during CA is not established within the scope of this experiment.

  • PDF

Studies on the Citric Acid Fermentation with Fungi (Part III) Citric Acid Fermentation with Selected Strains (사상균에 의한 구연산발효에 관한 연구 (제III보) 선정균에 의한 구연산발효)

  • 성낙계;김명찬;심기환;정덕화
    • Microbiology and Biotechnology Letters
    • /
    • v.8 no.3
    • /
    • pp.181-191
    • /
    • 1980
  • For the purpose of studies on the citric acid production, some experiments were carried out with isolated strains. The results obtained were as follows. 1) The optimal culture media of the strain M-80 in surface culture contained 140g of sucrose, 3.0g of (N $H_4$)$_2$S $O_4$, 1.5g of K $H_2$P $O_4$, 0.2g of MgS $O_4$.7$H_2O$, 3.0mg of F $e^{++}$, 1.0mg of Z $n^{++}$, 0.5N HCI to a pH of 5.0 and distilled water to 1.0 liter; and that of the strain M-315 in surface culture contained 140g of sucrose, 2.0g of N $H_4$N $O_3$, 1.0g of K $H_2$P $O_4$, 0.25g of MgS $O_4$. 7$H_2O$, 2.0mg of F $e^{++}$, 2.0mg of Z $n^{++}$, 0.05mg of C $u^{++}$, 0.5N HCI to a pH of 4.5 and distilled water to 1.0 liter. While that of the strain M-315 in submerged culture contained 140g of sucrose, 2.5g of N $H_4$N $O_3$, 1.5g of K $H_2$P $O_4$, 0.3g of MgS $O_4$. 7$H_2O$, 3.0mg of F $e^{++}$, 0.1mg of C $u^{++}$, 0.5N HCI to a pH of 4.5 and distilled water to 1.0 liter. The optimal temperature and size of inoculum were mostly 28-3$0^{\circ}C$, 10$^{7}$ -10$^{8}$ spores/50ml, respectively. 2) Through the course of citric acid production, the growth of strains had nearly been completed, pH value was rapidly decreased below 2.0 and the content of sugar was also reduced, while the accumulation of citric acid in media was remarkably begun in about 3-4 days. The yields of citric acid generally reached the maximum level in 8-10 days in surface or submerged fermentation process. 3) Methanol was effective citric acid production when they were added to fermentation media. In the case of surface culture, by addition of 2% (strain M-80), 3% (strain M-315), the yields of citric acid was increased 6.5%, 20.6%, respectively and 5.0% yield was increased by addition of 3% methanol in submerged culture media of the strain M-315. 4) Chromatography analysis of culture broth after fermentation under optimal culture conditions detected that the majority of acid in media was citric acid. 72.1mg/ml, 98.1mg/ml, of citric acid were determined in surface culture media by strains of M-80, M-315, and 59.8 mg/ml of citric acid was contained in the submerged culture media by the strain M-315. strain M-315.

  • PDF

The Analysis of the Genetic Variance and Combining Ability in some Quantitative Characters by Daillel Crosses of the Silkworm, Bombyx mori L. (이면교잡에 의한 가잠의 몇가지 실용형질의 유전변이와 조합능력 분석)

  • 장창식;손해룡
    • Journal of Sericultural and Entomological Science
    • /
    • v.27 no.2
    • /
    • pp.7-19
    • /
    • 1985
  • The genetic variances and combinding ability of some useful characters were analyzd on sixty four combinations in the 8$\times$8 diallel cross using the four Japanese races(Jam 107, Jam 113, Jam 117 and Jam 119) and the four Chinese races(Jam 108, Jam 114, Jam 118 and Jam 120). The eight quantitative characters were the total larval stage period(TP), the fifth larval instar period(FP), the female cocoon total weight(FW), the male cocoon total weight(MW), the female cocoon layer weight(FL), the male cocoon layer weight(ML), the female cocoon layer ratio(FR), and the male cocoon layer ratio(MR). The results were as follows: The analysis of the genetic variance and the combining ability in the TP and the FP. In TP and FP, h2N was less than h2B. The GCA, SCA and RCA were at a high significant level. Hl/D and (Hl/D)1/2 ere large. The heterosis were small minus. E and D were large. The r was in the positive direction, because the recessive genes were mainly expressed as a short rearing periods. The regressions of the characters were passed below 0 point, because the characters in the TP and the FP were appeared overdominant. The order of the dominance in the TP of the parents were in the order of Jam 119>Jam 113>Jam 117>Jam 108>Jam 120>Jam 114>Jam 107>Jam 118, and that in the FP of the parents were followed in the orders of Jam 117>Jam 113>Jam 108>Jam 114>Jam 119>Jam 107>Jam 120>Jam 118. The analysis of the genetic variance and the combining ability of the FW and the MW. In the FW and the Mw, h2N was less the h2B. The GCA and SCA were large but RCA was little. Hl/D and (Hl/D)1/2 in the parents were large. Heterosis was large. E was appeared large in the FW, and small in the MW. D was small. The r was of the minus direction, because the dominance genes were less expressed. The regression of the these characters were padded below 0 point, because the characters in FW and MW were appeared overdominant. The orders of the dominance in the FW of the parents were as the order of Jam 107>Jam 108>Jam 119>Jam 113>Jam 114>Jam 120>Jam 117>Jam 118, and in the MW of them in the order of Jam 114>Jam 120>Jam 108>Jam 113>Jam 107>Jam 119>Jam 117>Jam 118. The analysis of the genetic variance and the combining ability of the FL and ML. In the FL and the ML, h2N was less than h2B. GCA and SCA were large. RCA was little. Hl/D and (Hl/D)1/2 ere large. Heterosis was large. The r was in the negative direction, because the dominance genes were less expressed. The regression of the characters of FL and ML were appeared overdominant. The dominance in the FL of parents ere in the order of Jam 120>Jam 114>Jam 119>Jam 119>Jam 118>Jam 107>Jam 117>Jam 113, and the ML of them in the order of Jam 114>Jam 108>Jam 120>Jam 117>Jam 118>Jam 107>Jam 119>Jam 113. The analysis of the genetic variance and combining ability of the FR and the MR. In the FR and the Mr, h2N was less than h2B. GCA was large. The SCA and RCA were little. In the FW, Hl/D was large but (Hl/D)1/2 was a little. In MR, Hl/D and (Hl/D)1/2 both were a littel. Heterosis was a little. E in the FR was in the negative direction, because the dominance genes were less expressed but that in the MR was the positive direction because the recessive genes were mainly expressed. The order of the dominance in the FR of the parents were in the order of Jam 117>Jam 114>Jam 108>Jam 120>Jam 118>Jam 119>Jam 107>Jam 113 and that in the MR these were in the order of Jam 114>Jam 117>Jam 108>Jam 118>Jam 107>Jam 119>Jam 120.

  • PDF

Microbiological Evaluation of Foods and Kitchen Environments in Childcare Center and Kindergarten Foodservice Operations (보육시설과 유치원 급식의 식품 및 환경 미생물의 오염도 평가)

  • Seol, Hye-Rin;Park, Hyoung-Su;Park, Ki-Hwan;Park, Ae-Kyung;Ryu, Kyung
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.38 no.2
    • /
    • pp.252-260
    • /
    • 2009
  • Whereas the numbers of childcare centers and kindergartens are increasing rapidly, systematic management to control the food safety of foodservice operation is not yet well established. Samples from 12 centers in Seoul and Gyeonggi Province were collected to assess the microbiological quality of 32 raw materials, 24 cooked foods, 76 food-contact surfaces (knives, cutting boards, dish towels and gloves), 17 employees' hands and 12 air-borne bacteria. The microbiological analyses were performed for aerobic plate counts (APC), Enterobacteriaceae, E. coli and 7 pathogens (B. cereus, C. jejuni, C. perfringens, L. monocytogenes, Salmonella spp., S. aureus, and V. parahaemolyticus). Among raw materials, E. coli ($1.39{\sim}2.08\;\log\;CFU/g$) were detected in 4 out of 6 meats and 7.46 log CFU/g of APC in tofu. High enterobacteriaceae levels of 4.23, 5.14 and 4.19 log CFU/g were found in cucumber salad, steamed spinach with seasonings and steamed bean sprout with seasonings, respectively. No pathogens were found in all samples except for C. perfringens detected from raw spinach and raw lotus root. Only APC and enterobacteriaceae were found in food-contact surfaces. Two of the 23 knives and three of the 24 kitchen boards showed over 500 CFU/$100\;cm^2$ of APC; also, APC levels (5.03 to 5.44 log CFU/g) were detected in 4 of the 12 dish towels. Only one glove showed Enterobacteriaceae (2.44 log CFU/glove) contamination. Enterobacteriaceae were found in 2 employees' hands ($2.37{\sim}4.44\;\log\;CFU$/hand) among the 16 employees. The contamination levels of air-borne bacteria were shown unacceptable in two (2.25 and 2.30 log CFU/petri-film/15 min) out of the 12 kitchen areas. These results suggest that the microbiological hazards in some foods and environments are not well controlled and thus a guideline should be provided to ensure the food safety in childcare center and kindergarten foodservice operations.

Evaluation of Korean Native Ducks on Production Efficiency Factor, Carcass Yield, Partial Meat Ratio and Meat Quality with Weeks (토종오리 대형종의 주령별 생산지수, 도체 수율, 부분육 비율 및 육질 평가)

  • Heo, Kang-Nyeong;Kim, Hak-Kyu;Kim, Chong-Dae;Kim, Sang-Ho;Lee, Myeong-Ji;Choo, Hyo-Jun;Son, Bo-Ram;Choi, Hee-Cheol;Lee, Sang-Bae;Hong, Eui-Chul
    • Korean Journal of Poultry Science
    • /
    • v.40 no.2
    • /
    • pp.121-127
    • /
    • 2013
  • This work was carried to evaluate production efficiency factor, carcass yield and meat quality with weeks of Large-type Korean native ducks. Korean native ducks (n = 90) from National Institute of Animal Science (RDA, Korea) were used in this work. Ninety ducks were divided into 6 groups (15 birds/group) and were fed with meat-type duck diets for 8 wk old. When ducks grew at specific wk (6, 7 and 8 weeks), 2 ducks per group were slaughtered at 6, 7 and 8 wk old. Production efficiency factor, carcass yield, partial meat and meat quality were researched in this work. There was no significant difference on livability with weeks, but body weight at 7 and 8 wk old was higher than that at 6 wk old (P<0.05). Feed conversion ratio at 6, 7 and 8 wk old were 2.25, 2.69 and 3.21, respectively, so there was significant difference with weeks (P<0.05). Production efficiency factor at 6, 7 and 8 wk old were 256.6, 199.8 and 153.0, respectively, so there was significant difference with weeks (P<0.05). Carcass yield at 8 wk old was higher than that at 6 and 7 wk old as 73.5% (P<0.05). Lightness at 6, 7 and 8 wk were 41.8, 39.0 and 38.1, respectively, and that at 6 wk old was the higher than other weeks (P<0.05). There was no significant difference on redness at 6, 7, and 8 wk old (P>0.05) and yellowness at 8 wk old was higher compared to other weeks (P<0.05). Cooking loss was the highest at 6 wk old as 31.6%, but water holding capacity was the highest at 8 wk old (P<0.05). There was no significant difference on shear force among weeks. pH at 6 wk old was the lower than that of other weeks as 5.84. Moisture content significantly decreased with weeks (P<0.05) and fat content at 8 wk was the highest as 1.88% (P<0.05). Protein content significantly increased with weeks until 20.9% at the age of 8 wk (P<0.05). Ash content at 7 and 8 wk old was the higher than that at 6 wk old (P<0.05). There was no significant difference on juiciness, tenderness, and flavor with weeks. Finally, these results may provide that shipping time at 7 wk old preferred to that at 6 and 7 wk old, but further research was needed because of deficiency of data.

Prophylactic and therapeutic studies on intestinal giant-cystic disease of the Israel carp caused by Thelophanellus kitauei II. Effects of physical and chemical factors on T. kitauei spores in vitro (향어의 장포자충(Thelohanellus kitauei)증의 예방 및 치료에 관한 기초적 연구 II. 물리화학적 요인이 장포자충 포자에 미치는 영향)

  • Lee, Jae-Gu;Kim, Jong-O;Park, Bae-Geun
    • Parasites, Hosts and Diseases
    • /
    • v.28 no.4
    • /
    • pp.241-252
    • /
    • 1990
  • In a basic attempt to develop the prophylactic and therapeutic measures on intestinal giantcystic disease of the Israel carp, C), prinks carpio nudum, the effects of physical and chemical factors on viability or survival of the spores of Thelchcnellus kiteuei were checked in vitro by means of extrusion test on the polar filament. When the fresh spores suspended with 0.45% and 0.9% scdium chloride solution and distilled water were laid at $5^{\circ}C$ and $28^{\circ}C$ for short terms, the extrusion rates increased until the 3rd day, meanwhile when son;e of them were suspended with Tyrode's solution at $-70^{\circ}C$ the rates increased gradually until the 8th day. Viabilities of the spores suspended with 0.9% saline and added antibiotics to the suspension at $5^{\circ}C$ for long terms lasted for 997 days and 1, 256 days (presumed values) at maximum, respectively. The spores suspended with distilled water at $28^{\circ}C$ for long terms survived 152.4 days, but the spores suspended with Tyrode's solution at $-70^{\circ}C$ for long terms showed almost the same viable pattern as early freezing stages up to 780 days. The spores suspended with Tyrode's solution, frozen at $-70^{\circ}C$ and thawed at $5^{\circ}C$, showed the highest rate of extrusion of the polar filament. In the case of frozen spores, the extrusion rates during heating tend to become higher in accordance with the increase of frozen period, and the critical points of 180 day-frozen spores to be killed were generally 78.5 hr. at $60^{\circ}C$, 23.4 hr. at $70^{\circ}C$, 189.1 min. at $80^{\circ}C$ or 10.5 min. at $90^{\circ}C$. The longer the spores were frozen, the more time was needed for the death of spores after thawing; 20 days-17.4 days, 100 days-33.2 days, and 400 days-37.8 days. The longer the spores were frozen, the more time was needed for the death of spores at a conventional when they were dried air drying condition, 540 days-23.5 days, 160 days-21.0 days, and 20 days-14.4 days. On the other hand, the longer the spores were frozen, the more spores were dead rapidly when they were irradiated with 10W UV-ray; 100 days-26.0 hr, 300 days-21.9 hr, and 540 days-13.9 hr. The time needed for killing 200 days-frozen spores by various disinfectants at 1, 000 ppd was 5.2 min. by calcium oxide, 10.4 min. by potassium permanganate, 27.8 min. by malachite green and 14.3 hr. by formalin. Transient inhibitory effects of the extrusion of the polar filament were observed by various antiprotozoal and antifungal agents in the descending order of ketoconazole. metronidasole and dapsone. The above results presume that full drying, followed by spraying CaO and maintaining sunny condition for a few days on the concrete bottoms of knish farm may be an effective method for the prevention of intestinal giant.cystic disease.

  • PDF