• Journal of Life Science
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• v.29 no.2
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• pp.198-201
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• 2019

Recently, it has been reported that Bacillus mojavensis possesses antifungal properties and plant growth-promoting activities, which are similar to the characteristics of siderophore. In this study, the siderophore produced by B. mojavensis was assessed using a solid phase extraction (SPE) cartridge and liquid chromatography quadrupole time-of-flight tandem mass spectrometry (Q-TOF MS/MS). After B. mojavensis was incubated in phenol medium for 16 hr and lyophilized, the sample was dissolved in water and loaded to an SPE cartridge to remove interferences. The cartridge was washed with 5% methanol in water and eluted with 2% formic acid in methanol sequentially. The eluted solution was evaporated under a stream of nitrogen gas and reconstituted in methanol. The reconstituted sample was filtered, and $1{\mu}l$ of the sample was assessed using Q-TOF MS/MS. The mass spectrometer was operated using the positive electrospray ionization mode. Based on the mass spectrum and tandem mass spectrum, the siderophore produced by B. mojavensis was bacillibactin, one of the catechol types of siderophore with a molecular weight of 882.2556. This siderophore analysis could provide a justification for the study of B. mojavensis as a functional food and for pharmaceutical applications.

• The Journal of the Korea institute of electronic communication sciences
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• v.17 no.1
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• pp.133-138
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• 2022

In this paper we present the system we produced to help workers at logistic centers and prevent accidents, in which they could get hurt. As a base we use the main control device, named Arduino uno, which measures the weight by using load cells. When operating with the system, by placing object on it, the system measures the weight of the object and keeps the highest point at the same height by lowering its board. This improves the convenience while placing and removing objects from the board. If the weight of the placed object is exceeding the set value the board will also lower itself to secure the safety. By using a line tracer system, the objects are being moved only on a set route with the goal to make it even more comfortable to use.