• Korean Journal of Plant Tissue Culture
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• v.27 no.6
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• pp.491-496
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• 2000

A RAG25 gene regulating flowering time was introduced into Codonopsis lanceolata through high efficiencies (ca. 90%) of plant regeneration. The leaf explants were immersed in YEP media containing Agrobacterium tumefaciens (pGA 1209) harboring RAG25 gene, and cocultivated for 3 days. After cocultivation, they were cultured in shoot inducing media (SIM), N2B2 (NAA 2 mg/L, BA 2 mg/L and kanamycin 20 mg/L) and N2B4 (NAA 2 mg/L, BA 4 mg/L and kanamycin 20 mg/L), and the putative transformants were regenerated. The introduction of nptII and RAG25 gene into Codonopsis lanceolata was confirmed by 0.7 kb and 0.6 kb bands from polymerase chain reaction and reconfirmed by Southern hybridization using PCR product of RAG25 gene.

• Journal of Advanced Navigation Technology
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• v.21 no.2
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• pp.193-199
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• 2017

In the paper, a dual band-notched monopole antenna is proposed for 2.4 GHz WLAN (2.4 ~ 2.484 GHz) and UWB (3.1 ~ 10.6 GHz) applications. The 3.5 GHz WiMAX band notched characteristic is achived by a pair of L-shaped slots instead of the previous U-shaped slot on the center of the radiating patch, whereas the 7.5 GHz band notched characteristic is achived by C-shaped strip resonator placed near to the microstrip feed line. The measured impedance bandwidth (${\mid}S_{11}{\mid}{\leq}-10dB$) is 8.62 GHz (2.38 ~ 11 GHz) which is sufficient to cover 2.4 GHz WLAN and UWB band, while measured band-notched bandwidths for 3.5 GHz WiMAX and 7.5 GHz bnad are 1.13 GHz (3.15 ~ 4.28 GHz) and 800 MHz (7.2 ~ 8 GHz) respectively. In particular, it has been observed that antenna has a good omnidirectional radiation patterns and higher gain of 2.51 ~ 6.81 dBi over the entire frequency band of interest.

• Bulletin of the Korean Chemical Society
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• v.18 no.1
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• pp.120-121
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• 1997

• KSBB Journal
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• v.15 no.2
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• pp.156-161
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• 2000

This study was designed to investigate the antioxidative activity on oxidation of human low density lipoprotein(LDL) of band 2 fractionated from culture broth of Streptomyces sp. BH-405. Antioxidative activity of band 2 obtained from fractionation of BH-405 culture purification was measured against $Cu^{2+}$ mediated human LDL oxidation by thiobarbituric acid reactive substance. $CuSO_4$ mediated oxidation of LDL was degraded at a much higher rate than native LDL. Band 2 at a concentration of 100 or 200 !lg/mL inhibited the oxidation of LDL induced by $CuSO_4$, The formation of conjugated dienes induced in the presence of 5 !1M CuS04 of the mouse macrophage and J744. The electrophoretic mobility of the LDL in addition of $200\mu\textrm{g}$ band 2 in the presence of $5\mu\textrm{m}$ $CuSO_4$ was lower than that of native LDL. LDL modified by copper mediated or cell mediated uptake was degraded by macrophage at much greater than native LDL, and band 2 was found as potential inhibitor of modification of 125I-labelled LDL by macrophage. phage.

• YAKHAK HOEJI
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• v.28 no.3
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• pp.139-147
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• 1984

L-PHA, a lectin having lymphoagglutinating activity but devoid of erythroagglutinability which is contained in Korean white kidney bean (Phaseolus vulgaris L.), was isolated and purified through several techniques such as ion exchange chromatography, hydroxyapatite column and affinity chromatography. Purified L-PHA was identified as a single band by polyacrylamide disc gel electrophoresis. The molecular weight of the L-PHA was estimated about 125, 000 daltons by polyacrylamide gel electrophoresis and it was turned out having one subunit (probably dimer of the Yachnin's model) of Mr~60, 000. Immunochemical studies also tried and these results reconfirmed the purity of this L-PHA.

• Korean Journal of Plant Tissue Culture
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• v.27 no.6
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• pp.441-445
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• 2000

In order to improve phosphate use efficiency of rice using phosphate transporter (PT), transgenic rice plants containing a tobacco PT gene were developed. Calli from Dongjinbyeo (Oryza sativa L.) were cocultured with A. tumefaciens LBA 4404 harboring PT gene. Multiplied calli were transferred to MS medium supplemented with 50 mg/L hygromycin, 500 mg/L carbenicillin, 2 mg/L kinetin, 0.1 mg/L NAA. After 2 weeks, hygromycin resistant shoots were obtained from the calli on the selection medium. The putative transgenic shoots were transferred to rooting MS medium supplemented with 250 mg/L cabenicillin. Plant regeneration rate from the calli was about 52%. Stable incorporation of the tobacco PT gene into rice genomic DNA was confirmed by PCR and Southern blot analysis.

• Korean Journal of Veterinary Research
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• v.36 no.1
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• pp.119-129
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• 1996

The present study was conducted to rapidly detect Listeria monocytogenes in raw milk. Specificity and sensitivity of polymerase chain reaction(PCR) technique, and direct PCR were examinded in raw milk, also were compared the calssical culture methods with PCR technique. This method used a pair of primers based on a unique region in the 16S rRNA sequence of L nomocytogenes. In the PCR specificity tests, each of the 10 strains of L monocytogenes tested gave a single 70-bp band. But the other six Listera spp tested gave negative results. Results of the sensitivity tests showed that as few as 2 CFU of L monocytogenes in pure cultures could be detected with 16S rRNA-based primers, L-1 and L-2. In different PCR cycles, a PCR product was detected with $10^3$ cells of L monocytogenes from 25 cycles to 50 cycles and the concentration of PCR products was cycle-dependent. Raw milk samopes added L monocytogenes cells gave negative results. However, these samplers gave a single 70-bp band by pretreatment of pronase, and PCR products were detected with $10^1$ cells of L monocytogenes. To detemine the most sensitive culture protocol to use in conjunction with the PCR assay, raw milk samples were inoculated with L monocytogenes at concentrations ranging from 1 to $5.7{\times}10^4CFU/ml$. PCR assays from Listeria enrichment broth(LEB) containing raw milk samples added L monocytogene EGD could dtect 10 cells in pronase-pretreated samples without incubation, and 1 cell of L monocytogenes in both 12 hr and 24 hr incubation, respectively. Isolation raw of PCR assays was similar to that of classical culture methods, but required time for detection of L monocytogenes could remarkably be reduced compare to culture methods.

• Proceedings of the KIEE Conference
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• 2007.07a
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• pp.1079-1081
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• 2007

An L-band Linear Accelerator System for E-beam sterilization is under construction for bio-technology application. The klystron-modulator system as an RF microwave source has an important role as major components to offer the system reliability for long time steady-state operations. A PFN line type pulse generator with a peak power of 71.5-MW, $7\;{\mu}s$, 285 pps is required to drive a high-power klystron. The high power pulse transformer has a function of transferring pulse energy from a pulsed power source to a high power load. The pulse transformer producing a pulse with a peak voltage of 275 kV is required to produce 30-MW peak and 60 kW average RF output power at the frequency of 1.3-GHz. We have designed the high power pulse transformer with 1:13 step-up ratio. The peak and average power capability is 71.5-MW (275 kV, 260 A at load side with $7\;{\mu}s$ pulse width) and 130 kW, respectively. In this paper, we present measurements and its analysis on the design parameters, and an initial test result as well as a design concept on the high-power pulse transformer.

• Korean Journal of Remote Sensing
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• v.15 no.4
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• pp.367-375
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• 1999

This paper describes the development, operations, and applications of ROCSAT-l and its Ocean Color Imager (OCI) remote-sensing payload. It is the first satellite program of NSPO. The satellite was successfully launched by Lockheed Martin's Athena on January 26, 1999 from Cape Canaveral, Florida. ROCSAT-l is a Low Earth Orbit (LEO) experimental satellite. Its circular orbit has an altitude of 600km and an inclination angle of 35 degrees. The satellite is designed to carry out scientific research missions, including ocean color imaging, experiments on ionospheric plasma and electrodynamics, and experiments using Ka-band (20∼30GHz) communication payloads. The OCI payload is utilized to observe the ocean color in 7 bands (including one redundant band) of Visible and Near-Infrared (434nm∼889nm) range with the resolution of 800m at nadir and the swath of 702km. It employs high performance telecentric optics, push-broom scanning method using Charge Coupled Devices (CCD) and large-scale integrated circuit chips. The water leaving radiance is estimated from the total inputs to the OCI, including the atmospheric scattering. The post-process estimates the water leaving radiance and generates different end products. The OCI has taken images since February 1999 after completing the early orbit checkout. Analyses have been performed to evaluate the performances of the instrument in orbit and to compare them with the pre-launch test results. This paper also briefly describes the ROCSAT-l mission operations. The spacecraft operating modes and ROCSAT Ground Segment operations are delineated, and the overall initial operations of ROCSAT-l are summarized.

• Journal of electromagnetic engineering and science
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• v.1 no.1
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• pp.18-23
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• 2001

A fast-sweep broadband FM reflectometer system has been successfully developed and operacted at the DIII-D tokamak, producing reliable density Profiles with excellent spatial (1 $\leq$ cm) and temporal resolution (~100 $\mu$ s). The system uses a solid-state microwave oscillator and an active quadrupler, covering full Q-band frequencies (33~50 GHz) and providing relatively high output power (20~60 mW). The system hardware allows fu11band frequency sweep in 10 $\mu$ s, but due to digitization rate limit on DIII-D, sweep time was limited to 75~100 $\mu$ s. Fast frequency sweep has helped to reduce density fluctuation effects on the reflectometer phase measurements, thus improving reliability for individual sweeps. The fast-sweep system with high spatial and temporal resolution has allowed to measure fast-changing edge density profiles during plasma ELMS and L-H transitions, thus enabling fast-time sca1e physics studies.