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Effects of Harvest Time and GA3 Treatment on the Germination Rate of Pennisetum alopecuroides for. erythrochaetum (채종 시기 및 GA3 처리가 적수크령 종자의 발아율 향상에 미치는 영향)

  • Lim, Chae-Shin;Oh, Ju-Youl;Seck, Yong-Cheol;Kim, Do-Hahn
    • Journal of agriculture & life science
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    • v.44 no.6
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    • pp.1-7
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    • 2010
  • This experiment was conducted to enhance horticultural utilization value of wild graminaceous species on rooftop gardening purpose. Seeds of Pennisetum were harvested 5 times at an interval of 2 weeks from 2 October to 25 November in 2008. Seeds were soaked into solution of $GA_3$ different concentrations and incubated at the condition of $20^{\circ}C$ and $25^{\circ}C$ each for 12 hrs. Germination rate of the after-ripening seeds was above 80% in the 17 and 31 October and 13 November harvest, but germination rate of seeds harvested in the 2 October and 25 November was below 70%. Fresh seed germination and early growth of shoot and root after germination were promoted by $GA_3$ treatment. Effect of $GA_3$ treatments on germinability of fresh seeds was more prominent in the seeds harvested on 2 October and 25 November.

Method for Scale Effect on Front of Flood Wave Occurred by Levee Breach (제방붕괴로 인한 홍수파 선단의 축척효과 보정 방안)

  • Jeong, Seokil;Kim, Sooyoung;Lee, Seung Oh
    • Proceedings of the Korea Water Resources Association Conference
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    • 2016.05a
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    • pp.461-461
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    • 2016
  • 이상기후로 인하여 제방붕괴에 대한 위험성이 높아지고 있고, 이로 인해 발생하는 제내지 내 붕괴 홍수파에 대한 연구가 증가하고 있다. 특히 홍수파 선단의 이동 속도 추정은 제내지의 위험성을 예측하고, EAP 등을 수립하는데 매우 중요한 자료가 될 수 있다. 제내지에서의 선단 홍수파에 대한 예측을 하기 위해서는 해당 지역의 하천특성, 수리특성 및 제방 등 수공구조물 특성뿐만 아니라, 제내지의 지형 및 토지이용 현황 등을 조사하여 수리실험이나 수치모의를 수행해야 하는 것이 일반적이다. 수치해석의 경우 수리실험에 비하여 경제성 및 효율성 측면에서 뛰어날 수 있으나, 제방 붕괴과정 및 메커니즘을 반영하기가 불가능하다. 이에 반해 수리모형실험의 경우 제방붕괴 양상 및 그 과정의 재현이 가능하기 때문에, 제내지에서의 선단홍수파 전파에 관한 보다 정확한 양상을 파악할 수 있을 것이다. 그러나 이러한 실험은 실제 크기의 하천이 아닌 축소모형 또는 개수로에서 수행되었기 때문에, 초기 홍수파의 전파가 축척효과(Scale effect)의 영향을 받을 가능성이 매우 크다. 특히 선단 홍수파의 경우 파가 진행되면서 수심이 낮아지고, 유속이 느려지기 때문에 그 영향은 점차 증대된다고 할 수 있다. 이에 본 연구에서는 이러한 초기 선단 홍수파의 표면장력 영향을 고려한 보정 계수 산정에 대한 연구를 수리실험을 통해 수행하였다. 연구는 크게 4가지 과정으로 구성되었다. 첫째는 농도-표면장력의 관계 규명이며, 둘째는 표면장력-홍수파 전파 속도 관계 규명이다. 셋째는 표면장력과 Weber Number(We)의 관계를 도출하는 것이며, 마지막은 We를 이용하여 한계조건을 제시하는 것이다. 실험은 계면활성제의 농도를 변화시켜가며, 얇은 유리관(D=1.0 mm)에서 물의 상승 높이 및 접촉각을 측정하여 농도와 표면장력의 관계를 측정하였으며, 이러한 결과를 바탕으로 물의 표면장력을 변화시켜 가며 $0.5(B){\times}0.3(L){\times}0.2(H)m$의 Head-tank에 설치된 수문을 빠르게 개방하여, 홍수파가 퍼지는 양상을 관측하였다. 홍수파의 이동 속도는 진행시간에 따라서 퍼짐 넓이를 측정하여 제곱근을 취하는 방식으로 평균속도를 산정하였다. 이러한 관계를 이용하여 표면장력이 없을 경우의 이동속도 및 ??를 도출하였다. 본 연구를 통해 홍수파 전파 특성에 대한 수리실험 자료를 바탕으로 Weber 수를 도출하여 표면장력의 영향을 받는지 여부를 판단할 수 있으며, 표면장력의 영향을 받을 경우, 제시된 결과를 이용하여 홍수파 선단의 속도를 보정할 수 있다. 본 연구 결과는 토대로 축소모형으로 수행되는 홍수파 전파에 대한 수리실험결과의 보정 기초자료로 매우 유용할 것으로 판단된다.

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Chemical composition and in vitro digestibility of corn stover during field exposure and the fermentation characteristics of silage prepared with microbial additives

  • Gao, Jun Lei;Wang, Peng;Zhou, Chang Hai;Li, Ping;Tang, Hong Yu;Zhang, Jia Bao;Cai, Yimin
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.12
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    • pp.1854-1863
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    • 2019
  • Objective: To effectively use corn stover resources as animal feed, we explored the chemical composition and in vitro digestibility of corn stover during field exposure and the fermentation characteristics of silage prepared with lactic acid bacteria (LAB) and cellulase. Methods: Corn ears including the cobs and shucks were harvested at the ripe stage. The corn stover was exposed in the field under natural weather conditions. Silages were prepared after 0, 2, 4, 7, 15, 30, and 60 d of exposure. Corn stover was chopped into approximately 1 to 2 cm lengths and then packed into 5 liter plastic silos. The ensiling density was $550.1{\pm}20.0g/L$ of fresh matter, and the silos were kept at room temperature ($10^{\circ}C$ to $25^{\circ}C$). Silage treatments were designed as follows: without additives (control), with LAB, with cellulase, and with LAB+ cellulase. After 45 d of fermentation, the silos were opened for chemical composition, fermentation quality and in vitro digestion analyses. Results: After harvest, corn stover contained 78.19% moisture, 9.01% crude protein (CP) and 64.54% neutral detergent fiber (NDF) on a dry matter (DM) basis. During field exposure, the DM, NDF, and acid detergent fiber (ADF) contents of corn stover increased, whereas the CP and water-soluble carbohydrate contents and in vitro digestibility of the DM and CP decreased (p<0.05). Compared to the control silage, cellulase-treated silage had lower (p<0.05) NDF and ADF contents. The pH values were lower in silage treated with LAB, cellulase, or LAB+cellulase, and lactic acid contents were higher (p<0.05) than those of the control. Silage treated with cellulase or LAB+cellulase improved (p<0.05) the in vitro DM digestibility (IVDMD) compared to that of the control or LAB-treated silage. Conclusion: Corn stover silage should be prepared using fresh materials since stover nutrients are lost during field exposure, and LAB and cellulase can improve silage fermentation and IVDMD.

Effects of Ginseng Saponins in Energy Metabolism, Memory, and Anti-neurotoxicity

  • Wang Lawrence C.H.;Lee Tze-fun
    • Proceedings of the Ginseng society Conference
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    • 2002.10a
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    • pp.55-65
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    • 2002
  • Ginseng has been used as a key constituent in traditional medicine prescriptions for centuries. Other than its well-known anti-stress and adaptogenic properties, ginseng has also been shown to be very effective in treating age-related deterioration in metabolic and memory functions. Although it is generally believed that the saponin (GS) fraction of the ginseng root accounts for the bioactivity of ginseng, a direct demonstration on which ginsenoside does what is still generally lacking. In the past decade, our laboratory has endeavored to identify the active GS components involved in energy metabolism, memory, and anti-neurotoxicity. To examine the ergogenic effects of GS in enhancing aerobic capacity, rats were subjected to either severe cold ($40^{\circ}C$ under helium-oxygen, two hours) or exercise workload $(70\%\;VO_{2}max,$ to exhaustion). Acute systemic injection (i.p.) of ginseng GS (5-20 mg/kg) significantly elevated both the total and maximum heat production in rats and improved their cold tolerance. However, pretreating the animal with the optimal dose (10 mg/kg) of GS devoid of $Rg_1\;and\;Rb_1$ failed to elicit any beneficial effects in improving cold tolerance. This indicates that either $Rb_1\;and/or\;Rg_1$ may be essential in exemplifying the thermogenic effect of GS. Further studies showed that only pretreating the animals with $Rb_1(2.5-5\;mg/kg),\;but\;not\;Rg_l,$ resulted in an increase in thermogenesis and cold tolerance. In contrast to the acute effect of GS on cold tolerance, enhancement of exercise performance in rats was only observed after chronic treatment (4 days). Further, we were able to demonstrate that both $Rb_1\;and\;Rg_1$ are effective in enhancing aerobic endurance by exercise. To illustrate the beneficial effects of GS in learning and memory, a passive avoidance paradigm (shock prod) was used. Our results indicated that the scopolamineinduced amnesia can be significantly reversed by chronically treating (4 days) the rats with either $Rb_1\;or\;Rg_1$ (1.25 - 2.5 mg/kg). To further examine its underlying mechanisms, the effects of various GS on ${\beta}-amyloid-modulated$ acetylcholine (ACh) release from the hippocampal slices were examined. It was found that inclusion of $Rb_1$ (0.1 ${\mu}M$), but not $Rg_1$, can attenuate ${\beta}-amyloid-suppressed$ ACh release from the hippocampal slices. Our results demonstrated that $Rb_1\;and\;Rg_1$ are the key components involved in various beneficial effects of GS but they may elicit their effects through different mechanisms.

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Utilization of Multiple Carbon Sources by Plant Cells

  • Lee, Taek-Kyun;Suh, Jung-Bin;Kim, Se-Hee;Lee, Sun-Min;Lee, Woo-Sung
    • Proceedings of the Zoological Society Korea Conference
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    • 1999.10b
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    • pp.11-11
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    • 1999
  • ;It has been reported that suspension-cultured rice cells grown on mixed carbon sources of glucose (GIc) and acetate exhibited diauxic growth in which acetate was the preferred carbon source (Lee and Lee, 1996). Carrot (Daucus carota L.) suspension cells, showing a diauxic growth very similar to that of rice cells, were used to delineate the mechanisms underlying this preferential use of acetate over GIc. Uptakes of both GIc and 3-0-methylglucose (3-0MG), a non-metabolizable GIc analogue, were similarly inhibited when acetate or butylate, weak acids which are capable of transporting protons into the cytosol, were present in the uptake assay mixture containing cells harvested during the GIc-utilizing second growth phase. Inhibition of GIc uptake by these weak acids was similar when equivalent experiments were carried out with isolated plasma membranes. It was further shown that Glc uptake, which requires a proper proton gradient across the plasma membranes, was inhibited during the first growth phase by acetate-mediated alkalization of growth medium and/or simultaneous acidification of cytosol. This study strongly suggests that Glc utilization in plant cells is inhibited by co-presenting carbon source(s) which can alter the proton gradient across the plasma membrane. We further examined diauxic growth in culture containing GIc and malate. Unlike the case in the culture with GIc and acetate, carrot cells used GIc first. Malate was utilized only after Glc is depleted from medium. These results indicate that GIc can be a preferred or less-preferred carbon source depending on the competing carbon source. It was noted that malate was not directly taken up by cells. Instead it was converted extracellularly into fumarate which was subsequently transported into cells. During the malate-growth phase malate uptake was negligible, and fumarate uptake was active and pH-sensitive. It was shown that fumarase released into medium was responsible for the extracellular conversion of malate into fumarate. An immunoblot experiments showed that fumarase antibody raised against Arabidopsis fumarase provided positive signals only in medium in malate culture, not in fumarate or GIc cultures. This study demonstrates the first example in that fumarase, a mitochondria marker enzyme, can be present in places other than mitochondria.ndria.

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Effect of sweeteners on the quality characteristics of ginger (Zingiber officinale Rosc) Jungkwa (감미료가 생강정과의 품질특성에 미치는 영향)

  • Lee, Yeong-Min;Lee, Hyeon-Jeong;Cho, Jeong-Seok;Choi, Ji-Young;Woo, Jin-Ho;Moon, Kwang-Deog
    • Food Science and Preservation
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    • v.24 no.3
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    • pp.406-412
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    • 2017
  • In this study the physicochemical properties and antioxidant capacities of ginger (Zingiber officinale Rosc) Jungkwa with different kinds of sweeteners were determined. Jungkwa made with different sugars (sugar, xylitol, honey or oligosaccharides) were compared in aspect of physiochemical properties, antioxidant activities, total phenolic contents and sensory evaluation. Moisture contents Jungkwa treated different kinds of oligosaccarides showed highest value, in the order of honey, xylitol and sugar. $L^*$ value of Jungkwa treated with xylitol was the highest, $a^*$ value of Jungkwa treated with honey, sugar JungKwa were higher then others. Free sugar contents of Jungkwa treated with sugar showed the highest value in sucrose, glucose and galactose. Jungkwa with xylitol showed lowest value in all free sugar contents. Hardness and chewiness of Jungkwa treated with xylitol showed the highest value. The antioxidant activity measured by DPPH and ABTS radical scavenging activity and total penolic content were the highest in Jungkwa treated with honey, followed by Jungkwa treated with xylitol, oligosaccharides and sugar. Appearance and color of oligosaccarides and honey treated Jungkwa were preferred. In ginger taste, sweetness, chewiness were highest in sugar treated Jungkwa (not significant difference in treatments). As a result, honey treated Jungkwa has higher antioxidant activity and quality than other sugar treatments.

Batch Variation and Pharmacokinetics of Oral Sustained Release Melatonin-loaded Sugar Spheres in Human Subjects

  • Lee, Beom-Jin;Ryu, Seung-Goo;Choi, Han-Gon;Kim, Chong-Kook;Parrott, Keith-A.;Ayres, James-W.;Sack, Robert-L.
    • Archives of Pharmacal Research
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    • v.20 no.6
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    • pp.555-559
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    • 1997
  • The three different batches of an oral sustained release melatonin (MT) delivery system were prepared by aqueous-based fluid-bed coating of the sugar spheres for the evaluation of in vitro release characteristics and plasma concentration profiles in human subjects. The MT contents in 20% coated sugar spheres of three batches (B1, B2 and B3) were $3.3{\pm}0.08$, $2.4{\pm}0.1$ and $2.5{\pm}0.13$ mg per gram of coated sugar spheres, respectively. The release profiles of three different batches had a very similar fashion. However, the release profiles of three different batches had a very similar fashion. However, the release half-lives $(T_{50%})$ of MT from B1, B2 and B3 was $3.70{\pm}0.2$, $5.2{\pm}0.2$ and $4.9{\pm}0.07h$, respectively. Plasma concentration profiles of sustained release 0.2mg melatonin-loaded sugar spheres containing 10% immediate release melatonin in gelatin capsules (B1 and B2) were then evaluated in human subjects. The in vivo plasma concentration profies of the two batches (B1 and B2) were very similar each other and located between the physiological endogenous ranges. The time to reach the peak concentration $(T_max)$ was more advanced in case of B1 when compared to B2. However, there was no statistically significant difference in the maximum concentration $(C_max)$ and the area under the curve (AUC) between B1 and B2. The AUC of melatonin-loaded sugar spheres containing 10% and 20% immediate release MT in human subjects had a good linearity between dose and AUC, regardless of the fraction of immediate release MT, indicating the first order elimination process of MT within these doses. The current oral sustained release MT delivery system may be utilized to treat circadian rhythm disorders if it is proven to be more clinically useful when compared to immediate release MT.

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Studies on the Cause of Injury by Continuous Cropping and Soil Conditioner Application on Red Pepper(Capsicum annuum L.) I. Studies on the Cause of Injury by Continuous Cropping of Red Pepper (고추의 연작(連作) 장해요인(障害要因)과 토양개량제(土壤改良劑) 시용효과(施用效果) I. 고추연작(連作) 장해실태(障害實態) 조사(調査))

  • Hwang, N.Y.;Ryu, J.;So, J.D.
    • Korean Journal of Soil Science and Fertilizer
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    • v.21 no.1
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    • pp.49-54
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    • 1988
  • The present experiment was conducted to investigate causes affecting the reduction of red pepper production in the continuous cultivation upland soil from 1985 to 1986 in Imsil Chonbuk Province. The results obstained are summarized as follows: 1. Area ratio of continuous cultivation 2 years was 12.7%, 3 years 6.8% and over 4 years 48.9%. 2. Soil hardness and volume of solid and liguid of red pepper continuous cultivation soil were higher than those of one year cultivation, while pH and content of organic matter of continuous cultivation soil were low. The exceeding optimum level of phosphorus and potassium appeared factors affecting and reduction of red pepper of continuous cultivation soil. 3. Microflora density in continuous cultivation soil was high but bacteria/fungi (B/F) and actinomycetes/fungi (A/F) ratio were low. 4. The density of soil nematodes in continuous cultivation soil were higher than that in one year cultivation soil, however, the steeper and better drainage soil lowered the density of nematodes. 5. Continuous cultivation over 4 years showed 14.3% of plants diseased by phytophthora while 0.7% in one year cultivation soil.

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Determining Kinetic Parameters and Stabilization Efficiency of Heavy Metals with Various Chemical Amendment (중금속 안정화제의 반응 매개변수 결정 및 중금속 안정화 효율성 평가)

  • Oh, Se-Jin;Kim, Sung-Chul;Kim, Tae-Hee;Yeon, Kyu-Hun;Lee, Jin-Soo;Yang, Jae-E.
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.6
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    • pp.1063-1070
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    • 2011
  • In this study, total of 5 different chemical amendments were evaluated for determining kinetic parameters and stabilization efficiency of heavy metals in aqueous phase. Standard solution of Cd and Pb ($100mg\;L^{-1}$) was mixed with various ratio of amendments (1, 3, 5, 10%) and heavy metal stabilization efficiency was monitored for 24hrs. All examined amendments showed over 90% of removal efficiency for both Cd and Pb except zerovalent iron (ZVI) for Cd (43-63%). Based on result of heavy metal stabilization efficiency, it was ordered as $CaCO_3$ > Dolomite > Zeolite > Steel slag > ZVI for both Cd and Pb in aqueous phase. For kinetic study, first order kinetic model was adapted to calculate kinetic parameters. In terms of reaction rate constants (k), zeolite showed the fastest reaction rate (k value from 0.4882 for 1% to 2.0105 for 10%) for Cd and ZVI (k value from 0.2304 for 1% to 0.5575 for 10%) for Pb. Considering reaction rate constant and half life for heavy metal stabilization, it was ordered as Zeolite > $CaCO_3$ > Dolomite > Steel slag > ZVI for Cd and $CaCO_3$ > Dolomite > Steel slag > Zeolite > ZVI for Pb. Overall result in this study can be interpreted that lime containing materials are more beneficial to remove heavy metals with high efficiency and less time consuming than absorbent materials.

Damage and vibrations of nuclear power plant buildings subjected to aircraft crash part I: Model test

  • Li, Z.R.;Li, Z.C.;Dong, Z.F.;Huang, T.;Lu, Y.G.;Rong, J.L.;Wu, H.
    • Nuclear Engineering and Technology
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    • v.53 no.9
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    • pp.3068-3084
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    • 2021
  • Investigations of large commercial aircraft impact effect on nuclear power plant (NPP) buildings have been drawing extensive attentions, particularly after the 9/11 event, and this paper aims to experimentally assess the damage and vibrations of NPP buildings subjected to aircraft crash. In present Part I, two shots of reduce-scaled model test of aircraft impacting on NPP building were carried out. Firstly, the 1:15 aircraft model (weighs 135 kg) and RC NPP model (weighs about 70 t) are designed and prepared. Then, based on the large rocket sled loading test platform, the aircraft models were accelerated to impact perpendicularly on the two sides of NPP model, i.e., containment and auxiliary buildings, with a velocity of about 170 m/s. The strain-time histories of rebars within the impact area and acceleration-time histories of each floor of NPP model are derived from the pre-arranged twenty-one strain gauges and twenty tri-axial accelerometers, and the whole impact processes were recorded by three high-speed cameras. The local penetration and perforation failure modes occurred respectively in the collision scenarios of containment and auxiliary buildings, and some suggestions for the NPP design are given. The maximum acceleration in the 1:15 scaled tests is 1785.73 g, and thus the corresponding maximum resultant acceleration in a prototype impact might be about 119 g, which poses a potential threat to the nuclear equipment. Furthermore, it was found that the nonlinear decrease of vibrations along the height was well reflected by the variations of both the maximum resultant vibrations and Cumulative Absolute Velocity (CAV). The present experimental work on the damage and dynamic responses of NPP structure under aircraft impact is firstly presented, which could provide a benchmark basis for further safety assessments of prototype NPP structure as well as inner systems and components against aircraft crash.