• The Korea Journal of Herbology
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• v.22 no.4
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• pp.29-34
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• 2007

Objective : Kyenegum(Galli Stomachichum Corium) has been popularly used long as the digestive. The purpose of this study was to investigate the enzymatic characteristic of Kyenegum crude enzyme. Methods : To evaluate of the enzymatic characteristic of Kyenegum, we examined the activity of Kyenegum crude enzyme from optimum solvent, optimum temperature and pH of crude Kyenegum extract. Futhermore, we examined the effects of NaCI and acidity of crude Kyenegum extract. Results : The Kyenegum was composed with crude protein about 20%, crude lipid 2%. The optimum Kyenegum dry condition, optimum extract solvent, optimum temperature and optimum pH were $4{\sim}6$ hours at $60^{\circ}C$, commercial apple vinegar, $50^{\circ}C$ and 2.0. Conclusion : The result suggests that the Kyenegum crude enzyme extract very strong enzyme in temperature, NaCl and acidity, respectively.

• The Korea Journal of Herbology
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• v.22 no.4
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• pp.21-28
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• 2007

Objectives : Kyenegum has been popularly used long as the digestive. The purpose of this study was to investigate the purification and characteristics of protease obtained from Kyenegum crude enzyme. Methods : Kyenegum protease was purified by precipitation with ammonium sulfate followed by SP-Spharose ion exchange chromatography. The molecular weight of Kyenegum protease was estimated by SDS-PAGE electrophoresis. Results : Kyenegum protease was 3,087 units/mg protein specific activity, 14.5 purification fold and 9.8 % recovery. The molecular weight of protease was estimated to be 18 kDa. The isoelectric point was pI 8.97 and values of Km and Vmax of its were 48 mg/mL and 2 units/min, respectively. Conclusion : The result suggests that the protease obtained from Kyenegum has excellent stability of temperature, acid and collagen substrate specificity.

• The Korea Journal of Herbology
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• v.26 no.4
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• pp.89-94
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• 2011

Objective : Kyenegum has been frequently used for characterizing digestive symptoms in the traditional and oriental medicines. This study was conducted to investigate the characteristics of extracts from velvet antlers using the 4 different kinds of extracting methods. Methods : The extracts of velvet antlers were extracted using a $65^{\circ}C$ DW (9hrs), a Kyenegum crude enzyme, a $121^{\circ}C$ DW (2hrs), and a Kyenegum protease. To evaluate the characteristic of velvet antler extracts, we examined the brix, soluble solid, amino acid, mineral composition, and collagen protein. Results : As a result of the comparisons of velvet antlers extracted by the traditional extraction and the crude enzyme of kyenegum, the brix and soluble solid showed the higher contents for kyenegum enzymes. Also, mineral contents of the extracted velvet antlers were higher, particularly in Ca and P for those. The contents of collagen protein, hydroxyproline and hydroxylysine, were found to be more than twice in kyenegum protease compared with other extracting methods. Conclusion : These results indicated that the Kyenegum crude enzyme and protease are very effective to extract of velvet antlers.

• Herbal Formula Science
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• v.6 no.1
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• pp.157-173
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• 1998

The following study looked at the effects of Hyangsayukgunjatang and Hyangsayukgunjatang-ga-kyenegum, moryobun on gastric ulcers in rats. In the 1st experiment, ulcers were induced by method of shay's pylorus-ligated in rats. Subjects were administered 2 kinds of medicine, Hyangsayukgunjatang and Hyangsayukgunjatang-ga-kyenegum, moryobun, which were taken orally. The quantity of gastric juice secretion, total acidity of gastric juice, HCI secretion and serumgastrin content were measured and tissues of gastric mucosa epithelium were observed. 1. The quantity of gastric juice secretion in Hyangsayukgunjatang-treated and Hyangsayukgunjatang-ga-kyenegum, moryobun-treated groups showed significant decrease incomparision with the control group. 2. In relation to total acidity of gastric juice, only Hyangsayukgunjatang -treated groupshowed significant decrease in comparison with the control group. 3. HCI secretion of Hyangsayukgunjatang-treated and Hyangsayukgunjatang-ga-kyenegum, moryobun-treated groups showed significant decrease in comparision with the control group. 4. Content of serum gastrin, which were present in Hyangsayukgunjatang -treated and Hyangsayukgunjatang-ga-kyenegum, moryobun- treated groups showed significant decrease in comparision with the control group. 5. The degree of ulcer occurrence & parietal cell numbers and chief cell numbers of Hyangsayukgunjatang-treated and Hyangsayukgunjatang-ga-kyenegum, moryobun-treatedgroups, which were observed by macrographic & histologic method showed signicant decreasein comparison with the control group. Hyangsayukguniatang-treated group prooved to be increasingly effective. From above the experiment results it can be concluded that Hyangsayukgunjatang will be more clinical effective on curing gastric ulcers induced by method of Shay's pylorus-ligated rather than that of Hyangsayukgunjatang-ga-kyenegum, moryobun.