• 제목/요약/키워드: Ku70

검색결과 280건 처리시간 0.039초

Suppression of Ku80 Correlates with Radiosensitivity and Telomere Shortening in the U2OS Telomerase-negative Osteosarcoma Cell Line

  • Hu, Liu;Wu, Qin-Qin;Wang, Wen-Bo;Jiang, Huan-Gang;Yang, Lei;Liu, Yu;Yu, Hai-Jun;Xie, Cong-Hua;Zhou, Yun-Feng;Zhou, Fu-Xiang
    • Asian Pacific Journal of Cancer Prevention
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    • 제14권2호
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    • pp.795-799
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    • 2013
  • Ku70/80 heterodimer is a central element in the nonhomologous end joining (NHEJ) DNA repair pathway, Ku80 playing a key role in regulating the multiple functions of Ku proteins. It has been found that the Ku80 protein located at telomeres is a major contributor to radiosensitivity in some telomerase positive human cancer cells. However, in ALT human osteosarcoma cells, the precise function in radiosensitivity and telomere maintenance is still unknown. The aim of this study was to investigate the effects of Ku80 depletion in the U2OS ALT cell line cell line. Suppression of Ku80 expression was performed using a vector-based shRNA and stable Ku80 knockdown in cells was verified by Western blotting. U2OS cells treated with shRNA-Ku80 showed lower radiobiological parameters (D0, Dq and SF2) in clonogenic assays. Furthermore, shRNA-Ku80 vector transfected cells displayed shortening of the telomere length and showed less expression of TRF2 protein. These results demonstrated that down-regulation of Ku80 can sensitize ALT cells U2OS to radiation, and this radiosensitization is related to telomere length shortening.

구증구포(九蒸九)에 의한 녹차 제조 I. 일반성분 분석 및 화학적 조성 (Korean Green Tea by Ku Jeung Ku Po′s I. Analysis of General Compositions and Chemical Compositions)

  • 전정례;박금순
    • 한국식품조리과학회지
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    • 제15권2호
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    • pp.95-101
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    • 1999
  • This study was carried out to prepare green teas by traditional roasting manufacturing process, Ku Geung Ku Po and to determine the quality charateristics of the green teas by examining the change of their main components induced during this roasting process. The content of total sugars in unroasted tea leaves was 7.82%. Its content of roasted teas was decreased by increasing the number of roasting and there was significantly differences between samples. The content of total sugars in 9th roasted green tea was 3.98%. The total nitrogen contents of green teas produced by this process were 4.96∼6.38%. The more the number of roasting and the less content of tannin in green teas. And the ratio of total nitrogen and tannin in tea leaves 21.97 but its ratio in 9th roasted tea was increased to 45.54. The ascorbic acid in tea leaves consisted in 1,820.3 mg/100g but its content was decreased by increasing the number of roasting process. Whereas the content of caffeine in green teas was not reduced significantly by this traditional method. Of all amino acids, green tea produced by Ku Jeung Ku Po was rich glutamic acid, lysine and aspartic acid. And especially, phenylalanine, which was rarely found in other green teas, was abundant in these green teas. The compositions of glutamic acid and methionine were increased as increasing the number of roasting process but those of aspartic acid and arginine decreased by these processing. The main fatty acids of Ku Jeung Ku Po green tea were linolenic acid, linoleic acid and palmitic acid and the contents of unsaturated fatty acid in green teas were composed over 70% of total fatty acid. And the fatty acid contents in green teas were not affected during Ku Jeung Ku Po's process. The contents of minerals in these teas was rich in the oder of potassuim. magnessuim and calciumim, and these contents were not observed the prominant change during the process.

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위성의 Ku 및 ka밴드을 이용한 남북한 통신인프라 구축 (A Study on the Communication Infrastructure of North and South Korea Using KU & KA Band in Satellites)

  • 서석철;이재완;황민호;고남영
    • 한국정보통신학회:학술대회논문집
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    • 한국해양정보통신학회 2001년도 춘계종합학술대회
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    • pp.605-609
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    • 2001
  • 최근 정보화시대의 남한은 유.무선분야의 통신서비스 및 통신인프라에 있어 세계적 수준에 도달한 반면, 북한의 통신인프라는 기본적 전화수요조차 충족하지 못하는 남한의 '70년대와 유사한 낙후된 상황에 있다. 정보통신분문의 남북한 교류.협력은 경제, 사회 모든 분야에서 교류·협력의 중심부문으로서 남북통합의 기본토대를 마련한다는 점에서 매우 중요하다. 따라서 남북한 통합에 대비 통신망 구축을 위한 인프라로서 위성통신망은 망구축이 용이하고, 언제라도_ 남북한 전지역의 서비스가 가능하다는 장점을 가지고 있어 북한지역의 통신인프라구축에 있어 최적의 대안이 될 수 있다.

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체비셰프 응답을 갖는 이중모드 공동 공진기 필터의 설계 및 제작 (Design and implementation of dual-mode cavity filter with achebyshev response)

  • 김상철;홍의석
    • 한국통신학회논문지
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    • 제21권2호
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    • pp.505-513
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    • 1996
  • 본 논문에서는 Chebyshev 응답을 갖는 이중모드 대역통과 필터를 Ku-band와 K-band에서 설계.제작하였다. 제작된 필터는 2개의 독립 직교 $TE_{113}$ 원형 공진 모드로 공진한다. 2개의 이중모드 필터는 4-pole Chebyshev함수 특성을 가지며, 각각 중심 주파수 12.5GHz, 대역폭 100MHz와 중심 주파수 19.25GHz, 대역폭 120MHz를 갖는다. 측정 실험 결과 12.5GHz 이중모드 필터는 통과 대역내에서 1.2dB의 삽입 손실과 65dB의 저지 대역 감쇠를 나타냈고 19.25GHz 필터는 1.55dB의 삽입손실과 70dB의 저지 대역 감쇠를 나타냈다. 이러한 실험 결과는 설계 사양과 잘 일치함을 알 수 있었다.

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Improvement of Thunderstorm Detection Method Using GK2A/AMI, RADAR, Lightning, and Numerical Model Data

  • Yu, Ha-Yeong;Suh, Myoung-Seok;Ryu, Seoung-Oh
    • 대한원격탐사학회지
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    • 제37권1호
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    • pp.41-55
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    • 2021
  • To detect thunderstorms occurring in Korea, National Meteorological Satellite Center (NMSC) also introduced the rapid-development thunderstorm (RDT) algorithm developed by EUMETSAT. At NMCS, the H-RDT (HR) based on the Himawari-8 satellite and the K-RDT (KR) which combines the GK2A convection initiation output with the RDT were developed. In this study, we optimized the KR (KU) to improve the detection level of thunderstorms occurring in Korea. For this, we used all available data, such as GK2A/AMI, RADAR, lightning, and numerical model data from the recent two years (2019-2020). The machine learning of logistic regression and stepwise variable selection was used to optimize the KU algorithms. For considering the developing stages and duration time of thunderstorms, and data availability of GK2A/AMI, a total of 72 types of detection algorithms were developed. The level of detection of the KR, HR, and KU was evaluated qualitatively and quantitatively using lightning and RADAR data. Visual inspection using the lightning and RADAR data showed that all three algorithms detect thunderstorms that occurred in Korea well. However, the level of detection differs according to the lightning frequency and day/night, and the higher the frequency of lightning, the higher the detection level is. And the level of detection is generally higher at night than day. The quantitative verification of KU using lightning (RADAR) data showed that POD and FAR are 0.70 (0.34) and 0.57 (0.04), respectively. The verification results showed that the detection level of KU is slightly better than that of KR and HR.

Co-Fe 석출 양극산화피막의 초기석출부에 석출한 Fe-C가 자기특성에 미치는 영향 (The Effects Influenced on the Magnetic Properties by Depositing Fe-C in the Bottom Extremity of the Co-Fe Electrodeposited Anodic Oxidized Films)

  • 강회우
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 1994년도 하계학술대회 논문집 C
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    • pp.1295-1297
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    • 1994
  • At 34 at% Co-Fe, the aluminum anodic oxidized (alumite) films of particle diameter $150{\AA}$ have large perpendicular anisotropy energy and high coercive force. However, for the samples of particle diameter larger than $450{\AA}$, the bottom of each particle forms abnormal part called branch-shaped different from that of particle diameter $150{\AA}$. In this case the magnetic anisotropy energy Ku was about zero at the compositions of 45 and 75 at% Co. Furthermore, at tile compositions from 50 to 70 at% Co, the values of Ku became negative value. We confirmed that Fe-C deposited the bottom of particle orients very strongly and it has a large influence upon the magnetic anisotropy energy.

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Output Improvement of a Magnetic Levitation Control System

  • Jung, Hae-Young;Na, Seung -You
    • 한국지능시스템학회:학술대회논문집
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    • 한국퍼지및지능시스템학회 1995년도 추계학술대회 학술발표 논문집
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    • pp.59-70
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    • 1995
  • Output performance improvement using fuzzy logic to the conventional control scheme for a magnetic levitation system is presented in this paper, Adverse characteristics of nonlinearity, unstability, system parameter variation, etc, in the levitation system are partially overcome by the general fuzzy control action. Using a PD type compensator, a coarse framework of output performance is provided to the levitation system. Then a fine regulation to the output performance requirement is obtained by the natural description of the control action in the form of fuzzy logic controller. This control action soothes the adverse characteristics of the levitation system. In this way a better output performance can be obtained in a real time experiment.

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