• 제목/요약/키워드: Korean tea-leaves

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HPLC를 이용한 하동 녹차의 Catechin류, Alkaloid류 분석 및 항산화능 측정 (Analysis by HPLC of Catechins, Alkaloids and Antioxidant Activities in Hadong Green Tea Leaves)

  • 이미희;이승언
    • 한국응용과학기술학회지
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    • 제30권4호
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    • pp.761-769
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    • 2013
  • 하동지역에서 생산되는 잎 녹차(우전, 세작, 중작, 대작)에 함유되어 있는 catechin류, alkaloid류 및 theanine를 HPLC를 이용하여 분석을 함과 동시에 녹차 추출물을 이용하여 총 페놀 물질과 항산화능을 측정하였다. Catechin류와 alkaloid류, theanine, 총 페놀 화합물의 함량은 물 추출물 보다 80% 알코올 추출물에서 더 높았다. 총 catechin과 alkaloid의 함량은 80% 에탄올로 추출한 우전(172.33 mg/g, 30.80 mg/g)에서 가장 높았다. Theanine의 함량도 80% 에탄올 추출물에서 높았고 55.36에서 37.48 mg/g의 범위였다. 녹차의 총페놀 화합물은 우전에서 가장 높았고, DPPH법, FTC법 및 TBA법을 이용한 항산화 활성 측정에서도 우전에서 높은 결과를 나타내었다.

Bioavailability and Efficiency of Ten Catechins as an Antioxidant

  • Shi, John
    • Preventive Nutrition and Food Science
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    • 제7권3호
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    • pp.327-331
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    • 2002
  • Tea is a pleasant, popular and safe beverage in the world. During the past decade, epidemiological studies have shown that tea catechins intake is associated with lower risk of cardiovascular disease. Tea provides a dietary source of health-promoting components to help humans reduce a wide variety of cancer risks and chronic diseases. The antioxidative activity of tea-derived catchins has been extensively studied. The antioxidant effect is a synergistic action between catechins e.g. EGCG, EGC, ECG, EC, pheophytins a and b, and other components in tea leaves, which aye more bioavailable for human body. Green tea has a Higher content of catechins than other kinds of tea. Green tea extract with hot water has high potential and more efficiency to reduce cancer risk than any other tea products or pure EGCG. Protein, iyon, and other food components may interfere with the bioavailability of ten catechins. Interaction of catechins with drug affects the cancer-preventive activity of some cancer-fighting medication. Further studies are required to determine the bioavailability of tea catechins and cancer-preventive functionality.

차나무의 병해 I. Collectotirchum theae-sinensis에 의한 차 탄저병 (I. Anthracnose of Tea Tree Caused by Collectotrichum theae-sinensis)

  • 박서기
    • 식물병과 농업
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    • 제1권2호
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    • pp.26-28
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    • 1995
  • Anthracnose of tea tree, Camellia sinensis, occurred severely at tea plantation of Boseung, Chonnam. Irregular, dark brown spots appeared on tips and margins of mature leaves in early stage. As the disease progresses, the lesions were covered with numerous, small, black, slightly raised pustules (acervuli). The causal fungus of anthracnose was identified as Colletotrichum theae-sinensis (Miyake) Yamamoto. Symptoms by C. theae-sinensis appeared around 20 days after artificial inoculation.

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Isolation of 3-Galloylprocyanidin B3, a Glucosyltransferase Inhibitor from the Korean Green Tea Leaves

  • Cho, Young-Je
    • Journal of Applied Biological Chemistry
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    • 제43권4호
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    • pp.273-276
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    • 2000
  • In the course of surveying the anti-plaque agents for dental caries prevention, the extract of Korean green tea leaves (KGTL) was tested for inhibitory activity against Streptococcus mutans adhering to glass surfaces in the presence of crude glucosyltransferase (GTase). The extracts of KGTL showed a positive inhibitory activity against GTase. The active compound was purified through Sephadex LH-20 and MCI gel CHP-20P columns. A positive reaction was shown in the anisaldehyde-$H_2SO_4$ test, which confirmed the condensed tannin. The inhibitory compound was identified as 3-galloylprocyanidin $B_3$ through IR, negative FAB-mass, and $^{1}H$-NMR spectroscopic analyses. Acetone extract and 3- galloylprocyanidin $B_3$ of KGTL showed inhibitory effect against GTase. The percent of inhibition was determinated to be 71.84% (P<0.01) with 10 mM 3-galloylprocyanidin B3. The 3-galloylprocyanidin $B_3$, which possessed a galloyl, showed a higher inhibitory activity against glucosyltransferase than monomeric (+)-catechin and procyanidine $B_3$ which had no galloyl group.

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Molluscicidal Effect of Eco-Friendly Agricultural Substances for Controlling Golden Apple Snails (Pomacea canaliculata, Lamarck)

  • Il Kyu Cho;Woo Young Cho;In Sun Cho;Heon Woong Kim;Seonghoon Hyeong;Jang Hyun Park;Young Sig Kim;Kil Yong Kim;Gi-Woo Hyoung
    • 한국환경농학회지
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    • 제42권4호
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    • pp.396-407
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    • 2023
  • The golden apple snail (Pomacea canaliculata) has been utilized as a natural and eco-friendly control of weeds in rice paddy fields. However, P. canaliculata can damage other crops. In this study, the effectiveness of plant extracts from various natural sources that are reportedly effective against pests in the control of P. canaliculata was investigated. The four plant extracts were effective against P. canaliculata and ranked in descending order as green tea seed (Camellia sinensis) > root of red spider lily (Lycoris radiata) > leaves of tobacco (Nicotiana tabacum) > root of sophora (Sophora flavescens). The mortality rate of P. canaliculata was increased using 200 to 2000 mg/kg of green tea seed powder. However, shrubby sophora root extract did not significantly increase the mortality rate. The LC50 and LC90 of green tea seed, tobacco leaves, shrubby sophora root, and red spider lily root were 900 and 2800 mg/L, 956 and 2320 mg/L, 2162 and 5325 mg/L, and 512 and 1054 mg/kg, respectively. The LC50 and LC90 of ground powder of C. sinensis, N. tabacum, S. flavescens and L. radiata were 248 and 646 mg/L, 403 and 733 mg/L, 409 and 905 mg/L, and 493 and 1141 mg/L, respectively. The findings indicate the remarkable control potency of green tea seeds against the golden apple snail. An organic material incorporating the four plant powders may help control green apple snail in an ecosystem-friendly manner.

2011년 남부지방 차나무 저온 피해 조사 (A Survey Low Temperature Damage of Tea Tree at South Korea in 2011)

  • 황정규;김용덕
    • 한국농림기상학회지
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    • 제14권4호
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    • pp.246-253
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    • 2012
  • 수분부족과 지속적인 저온현상으로 우리나라 주요 차 생산 지역에 막대한 피해를 입혔는데, 이번 동해는 체내 수분부족으로 잎이 건조되어 퇴색된 녹색을 띄다가 고사하는 청고현상이 먼저 발견되었고, 대부분의 차(茶)재배지역에 피해를 입혔다. 이기간 동안 평균기온은 $0.2^{\circ}C$로 전년대비 $-2.7^{\circ}C$ 낮았고 2011년 01월과 02월의 최저기온의 평균기온은 $-5.6^{\circ}C$로 전년대비 $-4.5^{\circ}C$ 낮았으며, 가장 낮은 기온이 관측된 날은 01월 18일 06시 55분으로 $-11.8^{\circ}C$로 관측되었으며 전년 최저기온 보다 $-5.5^{\circ}C$ 낮게 관측되었다. 2010년 12월부터 2011년 02월까지 월동기간 동안 강우량은 104mm 밖에 되지 않아 전년보다 306mm 적게 내렸다. 색도 측정에서는 모든 지역에서 2012년에 비해 2011년도에서 L, a, b값 모두 높게 나왔고 수분함량 또한 2012년보다 2011년 잎의 수분함량이 낮게 나타났으며, Trypan blue분석에서는 저온 스트레스를 받지 않는 정상 차나무 잎에서는 세포사멸 현상이 거의 관찰되지 않았으나 저온 스트레스를 받은 청고피해 잎은 부분 세포사멸이 되어 부분 염색이 일어났으며, 적고피해 잎에서는 대부분 세포사멸이 일어나 대부분 염색이 되는 것을 확인할 수 있었다.

Seasonal Occurrence and Development of Gray Blight of Tea Plants in Korea

  • Koh, Young-Jin;Shin, Gil-Ho;Hur, Jae-Seoun
    • The Plant Pathology Journal
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    • 제17권1호
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    • pp.40-44
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    • 2001
  • Disease occurrence and development of gray blight of tea (Camellia sinensis) were investigated. Higher incidences and more severe damage by gray blight were found in Japanese tea variety Yabukita than the Korean local variety. In Yabukita, Pestalotiopsis longiseta was more frequently observed on the diseased leaves than P. theae but vice versa in the Korean local variety. This indicates that there was the varietal difference in the distribution of fungal species of gray blight pathogens. Both varieties were most severely damaged during the third harvest period with weather conditions of high temperature and humidity favorable to the disease. Presence of the tea brown blight fungus Glomerella cingulata on the margin of gray blight lesion at the late stahe suggested that the pathogenic fungi of tea gray blight were replaced by the brown blight fungus during the disease development.

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차광정도(遮光程度)가 차엽(茶葉)의 아미노산(酸), 무기성분(無機成分), 지방산(脂肪酸) 함량(含量)에 미치는 영향(影響) (Shading Effect on the Content of Free Amino Acids, Minerals, and Fatty Acids in Tea Leaves(Camellia sinensis O. Kuntze))

  • 박장현;최형국;김광식
    • 한국토양비료학회지
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    • 제29권3호
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    • pp.288-296
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    • 1996
  • 차광도(遮光度)가 다른 재배환경이 다엽의 무기성분, 유리아미노산. 지방산(脂肪酸) 함량에 미치는 영향(影響)을 검토한 결과는 다음과 같다. 전질소(全窒素) 함량(含量)은 95%차광구(遮光區)에서 5.49%로 가장 높았고 총 아미노산의 경우 차광처리구가 무처리구에 비해 함량이 더 많았고, 24670-30210mg/kg, Theanine은 95%차광구에서 15580 mg/kg으로 가장 많았고, 무처리구가 12470 mg/kg으로 가장 함량이 적었다. $NH{_4}^+$, $K^+$, $Mg^{+{+}}$는 차광구에서 함량이 높았고, $Ca^+$는 무처리구에서 함량이 높았다. 지방산 함량은 55% + 95%처리구가 27990 mg/kg으로 가장 많았고, 무처리구가 24350 mg/kg으로 가장 적었으며, 다엽의 지방산은 $C_{18:3}$, $C_{18:2}$, $C_{18:0}$, $C_{18:1}$, $C_{18:0}$ 순으로 함량이 많았다. 차광정도에 관계 없이 차광처리구가 무처리구에 비해 차엽 품질이 향상 되었으며, 특히 95% 차광구에서 품질이 가장 양호 하였다.

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한국산 녹차, 우롱차 및 홍차가 카드뮴에 중독된 흰쥐 간조직의 항산화적 해독작용에 미치는 영향 (Effects of Korean Green Tea, Oolong Tea and Black Tea Beverage on the Antioxidative Detoxification in Rat Poisoned with Cadmium)

  • 윤연희
    • Journal of Nutrition and Health
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    • 제27권10호
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    • pp.1007-1017
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    • 1994
  • In order to investigate the effect of Korean green tea, oolong tea and black tea beverage on the antioxidative detoxification in cadmium(Cd) poisoned rat liver, male Sprague-Dawley rat weighing 143$\pm$3.2g were divided into control and experimental groups. The experimental groups were fed standard diet containing 40ppm Cd and were given distilled water(CD), 5% black tea(BT), oolong tea(OT) and green tea(GT), respectively. Tea beverages were extracted from 5G dry leaves of teas in 100ml hot distilled water by the treatment at 85$^{\circ}C$ for 3 min. Liver xanthine oxidase(XOD) activity was increased by the administration of Cd except GT group. Liver superoxide dismutase(SOD), glutathione peroxidase(GSH-px), glutathione S-transferase(GST) activities were decreased by te administration of Cd but did not decreased by the administration of green tea(in GT group). Vitamin E and reduced glutathione contents were significantly decreased in Cd administered groups. Liver lipid peroxide value in Cd administered groups were increased compared to control group, but was not increased in GT group. Serum glutamic oxaloacetic transaminase(GOT) activities in CD, OT, BT groups were higher than control, but that in GT group was similar to control group. Serum glutamic pyruvic transaminase(GPT) activity was not significantly different among various groups. It was concluded that green tea might alleviate peroxidative damage in Cd-administered rat liver by reinforcing antioxidative detoxification system.

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녹차 염색 견포의 카테킨 함량 및 색상변화 (Catechins Content and Color Values of Silk Fabrics Dyed with Korean Green Tea Extracts)

  • 손지현;이명선;천태일
    • 한국염색가공학회지
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    • 제18권1호
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    • pp.10-19
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    • 2006
  • Despite several recent attempts to measure the concentration of individual catechins by HPLC, it has not been so easy to separate catechins accurately. The aim of the present work is to provide a proper condition for separating each component of catechins by HPLC analysis, and also to evaluate the experimental variables including color differences, and metal ion contents after dyeing and mordanting. Four kinds of Catechins, (-)-epicatechin(EC), (-)-epicatechin gallate(ECG), (-)-epigallocatechin(EgC), (-)-epigallocatechin gllate(EgCG) were isolated from the residues after dyeing. Catechins in Korean green tea leaves are richer when e tea leaves are younger. Higher concentration of catechins owes it to e way it is processed. The contents of catechins adsorbed in silk fabrics after dyeing were in order of EGCG>ECG>EGC>EC. We have found $68\%$ uptake of EGCG, and 116.8mg of EGCG in the silk fabrics after it was dyed with $1\%$ Korean green tea extracts. The absorbance intensity and K/S values of dyed silk fabrics were increased with dyeing temperature and time. Only the surface color of the silk fabric dyed with green tea extracts was yellowish red, but it changed from yellowish red to red with an increase in the mordant concentration. Post-mordanted silk fabrics with ferrous sulfate changed from yellowish red to red and purple color shade. In a practical evaluation, there is no significant change in color after twenty times of the continuous dry cleaning process. However, post-mordanted fabrics with ferrous sulfate faded the brightness of color after dry cleaning. Dyeing silk fabrics with a Korean tea extract reduced the metal ion contents in the silk fabrics when compared to the untreated silk fabrics. Metal contents in silk fabrics dyed and post-mordanted with $1\%$ each metal solution were 0.194 ppm for Aluminum, 1.601ppm for Copper, and 0.334 ppm for Iron. After the post-mordanting process, the heavy metal ion absorption increased, which was mainly attributed to the catechins and polyphenols in dyed silk fabrics.