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INHIBITORY EFFECT OF Er:YAG LASER ON THE GROWTH OF STREPTOCOCCUS MUTANS (Er:YAG 레이저 조사가 Streptococcus mutans의 증식억제에 미치는 효과)

  • Song, Gwang-Chul;Lee, Chang-Seop;Lee, Sang-Ho;Lee, Nan-Young
    • Journal of the korean academy of Pediatric Dentistry
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    • v.30 no.1
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    • pp.15-24
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    • 2003
  • The purpose of this study is to investigate the sterilization effect of Er:YAG laser against the intraoral acid producing bacterium, S. mutans, by irradiating the culture solution containing S. mutans KCTC 3065 with Er:YAG laser having a $650{\mu}m$ diameter beam through the non-contact method. We obtained the following results after examining the temperature changes of the culture solution, numbers of bacterial colonies, and acid-producing ability and attaching ability on teeth by measuring the amount of extracellular polysaccharide produced by S. mutans. The number of bacterial colony was decreased in $10{\mu}l$ culture solution irradiated with laser in overall compared to the control solution. The number decreased as the irradiation intensity and pulse repetition rate were larger and as the exposure time was increased. However, it did not change significantly in $100{\mu}l$ culture solution compared to the control solution. Although the acid-producing ability of S. mutans was inhibited for a certain duration after laser irradiation in 10r1 bacterial culture solution, it did not change in $100{\mu}m$ solution compared with the control solution. The amount of extracellular polysaccharide synthesized by S. mutans was partially decreased through laser irradiation in $10{\mu}m$ culture solution but did not change in $100{\mu}m$ culture solution. Based on these findings, we concluded that Er:YAG laser has an sterilization effect on S. mutans in which we presume that the mechanism is through the heat effect rather than the mechanical effect from development of ultrasound.

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IN VITRO STUDY OF CARIOGENIC POTENTIAL OF INFANT CONFECTIONARIES (유아용 과자의 우식유발능에 관한 생체 외 연구)

  • Song, Ji-Hyon;Lee, Kwang-Hee;Kim, Dae-Eup;Ra, Ji-Young;Lee, Dong-Jin;An, So-Youn
    • Journal of the korean academy of Pediatric Dentistry
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    • v.35 no.1
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    • pp.39-46
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    • 2008
  • The purpose of this study was to assess the cariogenic potential of infant confectionaries. In vitro, as compaired with 10% sucrose solution and whole bovine milk. Buffering capability were determined by amount of 0.1N lactic acid consumed to titrate the 50ml specimen solutions to pH 4.0. The pH of the specimen solution inoculated by streptococcus mutans was measured by pH meter and the surface microhardness tester, before and after 48 hours incubation. The buffering capacity of infant confectionaries was higher than that of sucrose solution and lower than that of milk, and there were significant difference between infant confectionaries(p<0.05). The pH of infant confectionaries after 48 hours incubation was similar to 10% sucrose solution, and there were significant difference between infant confectionaries and milk(p<0.05). The microhardness change of primary tooth enamel of infant confectionaries group after 48 hours incubation was similar to that of 10% sucrose solution, and there were significant difference between infant confectionaries and milk(p<0.05). In conclusion, infant confectionaries seemed to have the ability to cause dental caries in primary teeth, and there were significant differences of cariogenic potential among infant confectionaries. Cooperative efforts of dentistry and manufacturers to reduce the cariogenic potential of infant confectionaries would be necessary to prevent the early childhood caries in children.

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CORRELATION OF CARIES ACTIVITY BETWEEN MOTHERS AND CHILDREN WITH CARIOGRAM AND EVALUATION OF CARIES RISK FACTORS (Cariogram을 이용한 모자간 우식활성도의 상관성 및 우식위험요소 평가)

  • Lee, Suk-Woo;Song, Je-Seon;Choi, Byung-Jai;Choi, Hyung-Jun;Lee, Jae-Ho
    • Journal of the korean academy of Pediatric Dentistry
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    • v.36 no.3
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    • pp.337-347
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    • 2009
  • Dental caries during infantile and early childhood period is a complex disease resulting from multiple caries factors. Streptococcus mutans(S. mutans) plays a critical role in the initiation of caries, and its early transmission through mothers provides a strong etiologic factor for future development of caries in the primary dentition. Therefore, early detection of caries risk factor is important for prevention of caries. Recent studies about etiologic factors of caries have brought advent of various tools for caries risk assessment in order to predict progress of caries more accurately. Cariogram is a common tool for caries risk assessment, which illustrates present caries risk assessment and correlation of caries risk factors for an individual graphically. The aim of this study was to assess if there is any correlation in the level of S. mutans and caries activity and to verify the effect of caries risk factors between children with age ranging from 3 to 5 years with severe early childhood caries(S-ECC) and their mothers using caries-related salivary test and Cariogram. The results of this study were as follows. 1. Children with S-ECC had a statistically higher level of Streptococcus mutans compared to caries-free children(p<0.05). 2. There was significant correlation between mothers and their children in the aspect of the level of Streptococcus mutans(p<0.05). 3. When caries activity was assessed using Cariogram, significant correlation between mothers and their children was found(p<0.05). 4. When each caries risk factor was evaluated for its affect on caries activity, host was more influential factor compared to microorganism and diet in children. Based on these results, we could conclude that assessing the level of S. mutans can be an effective tool for predicting possibility of caries formation in future. Since prediction of future caries progression and evaluation of caries risk factor became possible with Cariogram, we may conduct early and preventive measures for treatment of caries.

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Characteristics of Intravenous Midazolam Sedation with Nitrous Oxide in Pediatric Dental Treatment (소아환자에서 midazolam 정주 및 아산화질소 흡입진정법 하 치과치료의 특성)

  • Kim, Hyuntae;Song, Ji-Soo;Hyun, Hong-Keun;Kim, Young-Jae;Kim, Jung-Wook;Jang, Ki-Taeg;Lee, Sang-Hoon;Shin, Teo Jeon
    • Journal of the korean academy of Pediatric Dentistry
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    • v.47 no.1
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    • pp.53-61
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    • 2020
  • Midazolam is a short-acting benzodiazepine that is widely used in pediatric dental sedation. However, its clinical effectiveness as an intravenous sedative agent in children has not been widely documented. A retrospective study was conducted to evaluate the efficacy and safety of intravenous midazolam and nitrous oxide inhalation sedation in pediatric dental treatment. The subjects were 115 patients (118 cases) who received dental treatment under intravenous midazolam and nitrous oxide inhalation sedation. Demographic factors, general health status, sedation time, midazolam and nitrous oxide dosage, and success rate of sedation were evaluated from electronic medical records. Behavioral management was the main reason of choosing sedation. Mean duration of sedation was 56.7 minutes for surgical treatment, and 74.4 minutes for restorative treatment. The initial dosage of intravenous midazolam was 0.051 ± 0.019 mg/kg. In 34 cases (28.8%), additional midazolam of 0.036 ± 0.057 mg/kg was delivered during the treatment. The concentration of nitrous oxide was maintained between 40% and 50%. The success rate of sedation was 99% (n = 117). In 1 case, laryngospasm occurred and the patient was reversed with benzodiazepine antagonist, flumazenil. Intravenous midazolam sedation with nitrous oxide was shown to be clinically effective for the dental treatment in children, if administered by trained personnel and patients are carefully selected in accordance with guidelines.

Differences of Perceptions between Pre-service and In-service Elementary School Teachers on the Prevention and Intervention of School Violence (학교폭력의 예방 및 대책에 관한 교대생과 초등교사의 인식 비교)

  • Song, Jae-Hong;Kim, Kwang-Soo;Park, Sung-Hee;Ahn, Ie-Hwan;Oh, Ik-Soo;Eun, Hyuk-Gi;Jeong, Jong-Jin;Cho, Bung-Hwan;Hong, Jong-Kwan;Hwang, Mae-Hyang
    • The Korean Journal of Elementary Counseling
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    • v.11 no.3
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    • pp.485-504
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    • 2012
  • The purpose of study was to analyze the differences of perceptions between pre-service and in-service elementary school teachers on the prevention and intervention of school violence. For this purpose, 459 student teachers and 289 in-service teachers has responded a questionnaire consisted of 12 core questions and 8 supplementary questions about the prevention and intervention of school violence. The findings of this study was as follows: First, in-service elementary school teachers perceived the problem of school violence more seriously than student teachers. Second, in-service elementary school teachers and student teachers perceived the lack of parents' concern of their children and the disturbed friendship as major causes to arouse school violence, and insisted to improve students' emotional self-control and social skills in order to prevent school violence. Third, in coping with students' violent behaviors they took the need to interview the student' parents and to invite them to meet each other halfway. Fourth, With government's comprehensive countermeasures against school violence they acknowledged the importance of policy alternatives but made doubt of their effectiveness. Finally, both in-service elementary school teachers and student teachers approved of a plan to assign the required subject of 'Prevention and Intervention of School Violence' to the student teachers, particularly student teachers approved more positively than in-service elementary school teachers.

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Enhanced High-Temperature Performance of LiNi0.6Co0.2Mn0.2O2 Positive Electrode Materials by the Addition of nano-Al2O3 during the Synthetic Process (LiNi0.6Co0.2Mn0.2O2 양극 활물질의 합성공정 중 나노크기 알루미나 추가에 의한 고온수명 개선)

  • Park, Ji Min;Kim, Daeun;Kim, Hae Bin;Bae, Joong Ho;Lee, Ye-Ji;Myoung, Jae In;Hwang, Eunkyoung;Yim, Taeeun;Song, Jun Ho;Yu, Ji-Sang;Ryu, Ji Heon
    • Journal of the Korean Electrochemical Society
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    • v.19 no.3
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    • pp.80-86
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    • 2016
  • High Ni content layered oxide materials for the positive electrode in lithium-ion batteries have high specific capacity. However, their poor electrochemical and thermal stability at elevated temperature restrict the practical use. A small amount of $Al_2O_3$ was added to the mixture of transition metal hydroxide and lithium hydroxide. The $LiNi_{0.6}Co_{0.2}Mn_{0.2}O_2$ was simultaneously doped and coated with $Al_2O_3$ during heat-treatment. Electrochemical characteristics of modified $LiNi_{0.6}Co_{0.2}Mn_{0.2}O_2$ were evaluated by the galvanostatic cycling and the LSTA(linear sweep thermmametry) at the constant voltage conditions. The nano-sized $Al_2O_3$ added materials show better cycle performance at elevated temperature than that of micro-sized $Al_2O_3$. As the added amount of nano-$Al_2O_3$ increased, the thermal stability of electrode also enhanced, but the use of 2.5 mol% Al showed the best high temperature performance.

The Variation of Leaf Form of Natural Populations of Quercus variabilis in Korea (굴참나무 천연집단(天然集團)의 엽형(葉型) 변이(變異))

  • Song, Jeong-Ho;Park, Mun-Han;Moon, Heung-Kyu;Han, Sang-Urk;Yi, Jae-Seon
    • Journal of Korean Society of Forest Science
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    • v.89 no.5
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    • pp.666-676
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    • 2000
  • For the study of morphological variation of Q. variabilis natural population in Korea, 19 populations were selected through the country in considering latitude, longitude, and geographical characters. Thirty trees were randomly selected from each population and 60 mature leaves were sampled from each tree. Four characters (leaf blade length, maximum blade width, petiole length, and vein number) were measured, and their ratios (the ratio of blade length to maximum blade width, the ratio of blade length to petiole length, the ratio of petiole length to vein number, upper 1/3 blade width to maximum blade width, and upper 1/3 blade width to lower 1/3 blade width) were calculated. 1. Analysis of variance for all leaf characters were significantly different among populations and among individuals within population. Contributions of variance among individuals within population in all the characters were higher than those among populations. Therefore, selection of plus trees may be preferable to desirable populations for breeding program of Q. variabilis. 2. Among principal component analysis for leaf characters, primary 2 principal components appeared to be major variables for leaf form of Q. variabilis because of the loading contribution of 80.5%. The first contribution component was petiole length/vein number and petiole length ; the second one was upper 1/3 blade width/maximum blade width, upper blade width/lower 1/3 blade width and vein number, respectively. 3. Latitude was positively correlated with blade length/maximum blade width and blade length/petiole length, but negatively correlated with petiole length/vein number, upper 1/3 blade width/maximum blade width, upper 1/3 blade width/lower 1/3 blade width, petiole length, and vein number. But, for longitude and altitude the former two traits and the later five traits exhibited the negative and positive correlation, respectively. 4. Cluster analysis using complete linkage method for leaf characters showed two groups to Euclidean distance 1.6. They were group I of population 1. 4, 5, and 13 and group II of population 2, 3, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, and 19. However, group II was divided again to Euclidean distance 1.3, that is a group including population 3, 7, 10, 14, 15, and 17(group II-1) and the other group comprising population 2, 6, 8, 9, 11, 12, 16, 18, and 19(group II-2). This cluster could be mainly observed due to difference among population in aspect (group I : NE, group II-1 : SE, and group II-2 : SW).

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An Analysis of Vegetation-Environment Relationships of Pinus densiflora for. erecta and Chunyang-type of Pinus densiflora Communities by TWINSPAN and DCCA (TWINSPAN과 DCCA에 의한 금강(金剛)소나무 및 춘양목(春陽木)소나무 군집(群集)과 환경(環境)의 상관관계(相關關係) 분석(分析))

  • Song, Ho Kyung;Kim, Seong Deog;Jang, Kyu Kwan
    • Journal of Korean Society of Forest Science
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    • v.84 no.2
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    • pp.266-274
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    • 1995
  • Vegetational data from 62 quadrats of Pinus densiflora for. erecta and Chunyang-type of Pines densiflora forests were analyzed by using two multivariate methods : TWo-way INdicator Species ANalysis(TWINSPAN) for classification and Detrended Canonical Correspondence Analysis(DCCA) for ordination. The dominant tree species of Pinus densiflora for. erecta communities were found in the order of Pines densiflora for. erecta, Quercus mongolica, Quercus variabilis, Lindera obtusiloba, Fraxinus rhynchophylla, and Rhus trichocapa. The dominant tree species of Chunyang -type of Pinus densiflora communities were Quercue variabilis. Quercue mongolica, Fraxiraus sieboldiana, Styrax obassia, and Quercus serrata. The forest vegetation of Pinus densiflora was classified into Quercars variabilis-Styrax obassia. Quercus variabilis Quercus variabilis-Quercus mongolica, and Quercue mongolica communities according to TWINSPAN. Pinus densiflora for. erecta community was distributed in the good nutrition area of total nitrogen. organic matter, $K^+$, $Ca^{{+}+}$, $Mg^{{+}+}$, and canon exchange capacity, while Chunyang type of Pinus densiflora community in the good nutrition area of $P_2O_5$. The relationship between the distribution of dominant communities for forest vegetation and soil condition in Pinus densiflora communities was investigated by analysing the elevation and soil nutrition gradients. Quercus mongolica community was distributed in the high elevation and good nutrition area of total nitrogen, organic matter, and ration exchange capacity, while Quercus variabilis community was distributed in the low elevation and poor nutrition area of total nitrogen, organic matter, and ration exchange capacity. Quercus variabilis Styrax obassia and Quercus variabilis-Quercus mongolica community was distributed en the medium elevation and medium nutrition area.

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Physicochemical Characteristics of the Tofu (Soybean Curd) Added Sorghum (Sorghum bicolor L. Moench) Powder (수수가루 첨가에 따른 두부의 이화학적 특성)

  • Woo, Koan-Sik;Ko, Jee-Yeon;Seo, Myung-Chul;Song, Seuk-Bo;Oh, Byeong-Geun;Lee, Jae-Saeng;Kang, Jong-Rae;Nam, Min-Hee
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.12
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    • pp.1746-1752
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    • 2009
  • This study was carried out to compare the physicochemical characteristics of the tofu (soybean curd) with the additions of sorghum powder (SP). With increasing amounts of sorghum powder, the yields of tofu were increased. The tofu made of 5 and 7% (w/w) addition rates of SP gave the similar yields with that of control. Moisture contents of the tofu made of roasted sorghum powder after steeping for 15 hours (WR-SP) were higher than those of the control and the tofu made of roasted sorghum powder after washing (SR-SP) without steeping. The dietary fiber contents of tofu were higher in the SP-added tofu than control. The potassium contents increased with SP addition rates, while calcium and magnesium decreased. With increasing SP addition rates, hardness of the tofu decreased. With increasing amounts of SP, the L- and b-values of tofu decreased, whereas a-value increased. The total polyphenol, flavonoid and tannin contents, and ascorbic acid equivalent antioxidant capacity in the tofu extracts increased with increasing SP rates. Antioxidant components and activity were the highest in the tofu made of roasted sorghum powder after washing without steeping with the addition rates of 3-10%.

Inhibitory Effect of Rhus Verniciflua Extract on Lipid Peroxidation and Inflammatory Cytokines during Endurance Exercise Training (지구성 운동시 옻나무 추출액의 지질과산화 및 염증성사이토카인 억제효과)

  • Lee, Youn-Kyung;Kwon, Oh-Seon;Song, Young-Ju;Kim, Sea-Hyun;Kim, Pan-Gi;Ryu, Sung-Pil
    • Journal of Korean Society of Forest Science
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    • v.99 no.1
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    • pp.102-110
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    • 2010
  • This study was performed to find out the inhibitory effect of Rhus Verniciflua extract on lipid peroxidation and inflammatory cytokines during endurance exercise training for 8 weeks in rats. For this study, Sprague-Dawley rats were divided into 4 groups; sedentary (SED), exercise training (TRA), RVS extract ingestion (RVE), and RVS extract ingestion and exercise training (RVE-TRA). TRA and RVE-TRA were trained on treadmill with increasing speed gradually and administered 10 mL/kg/d of Rhus Verniciflua extract orally to RVE and RVE-TRA. In order to analyze antioxidant function, blood SOD (superoxide dismutase), GSH-Px (glutathione peroxidase), and MDA (malondialdehyde) were examined. And, analysis of inflammatory cytokines were examined using IL-6 (interleukin-6), TNF-${\alpha}$ (tumor necrosis factor-alpha), CRP (C-reactive protein), and NO (nitric oxide). SOD in TRA was significantly higher than SED and RVE (p<0.05), and RVE-TRA was highest among the groups (p<0.05). The MDA content of TRA, RVE and RVE-TRA were significantly lower than SED. GSH-Px activity of SED was significantly lower than other groups (p<0.05). IL-6 and TNF-${\alpha}$ content of RVE and RVE-TRA were significantly lower than SED and TRA (p<0.05). CRP concentration of SED was the lowest among groups (p<0.05). Finally, NO concentration of SED and TRA were higher than RVE and RVE-TRA (p<0.05). These results suggested that it is efficient for rats to reduce lipid peroxidation and induce anti-inflammatory by taking RVS extract during exercise training. Afterwards, if studies on the properties of RVS extract can be made with various ways, use of Rhus Verniciflua trees might be made widely which are growing naturally in mountains in Korea.