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Experimental Pathogenesis of Pullorum Disease with the Local Isolate of Salmonella enterica serovar. enterica subspecies Pullorum in Pullets in Bangladesh

  • Haider, M.G.;Chowdhury, E.H.;Khan, M.A.H.N.A.;Hossain, M.T.;Rahman, M.S.;Song, H.J.;Hossain, M.M.
    • Korean Journal of Poultry Science
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    • v.35 no.4
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    • pp.341-350
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    • 2009
  • The research work was carried out to study the pathogenesis covering the clinical signs, gross and histopathological lesions in different organs, and reisolation and identification of the organisms after experimental infection with the local isolate of Salmonella enterica serovar. enterica subspecies (S.) Pullorum at different time interval of the experiment during the period February 2006 to December 2006. One hundred pullets (seronegative to S. Pullorum of 12 weeks age were purchased and divided into 5 (A, B, C, D and E) groups and each group consisted of 20 birds. Four groups (A, B, C and D) were infected orally with a dose of $10^6\;CFU$, $10^7\;CFU$, $2{\times}10^7\;CFU$, $10^8\;CFU$ of S. Pullorum, respectively, and one group (E) was treated as uninfected control. The used methods were necropsy and histopathology, culture of bacteria, staining and biochemical test of Salmonella. Five birds from each group were randomly selected and sacrificed $1^{st}$ week, $2^{nd}$, $3^{rd}$ and $4^{th}$ weeks of post infection (PI). From all the groups, the bacteriological samples (crop, liver, lung, heart, spleen, bile duodenum, ceca and blood) were collected with pre enriched in buffered peptone water in sterile poly bags. Liver, lungs, heart, spleen, intestine, etc. were collected in 10% buffered-formalin for histopathological examination. No clinical signs, gross and histopathological lesions were found in control group and no S. Pullorum was reisolated. Clinical sign of experimentally infected with S. Pullorum in pullets were loss of appetite (100%), slight depression (75%), ruffled feathers (85%), diarrhea (60%) and loss of weight (100%) in chickens. The feed intake and body weight at different weeks after PI differed significantly (p<0.01) among the groups. Grossly, the highest recorded lesion was button-like ulcer in the ceca (80%) and the lowest was white nodules in lungs (1.25%). S. Pullorum were reisolated from crop (91.25%), liver (91.25%), lung (83.75%), heart (71.25%), spleen (87.75%), bile (33.25%), duodenum (92.50%), ceca (97.50%) and from different group of infection (61.25%). The highest microscopic findings were intestinal and cecal mucosa and submucosa exhibited infiltration of mononuclear cells and congestion (96.25%), and the lowest finding was nodule formation in the lungs (3.75%). The pattern of the disease production by local isolate of S. Pullorum in Bangladesh is almost similar with other isolates in different countries.

Study of Prediction Model Improvement for Apple Soluble Solids Content Using a Ground-based Hyperspectral Scanner (지상용 초분광 스캐너를 활용한 사과의 당도예측 모델의 성능향상을 위한 연구)

  • Song, Ahram;Jeon, Woohyun;Kim, Yongil
    • Korean Journal of Remote Sensing
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    • v.33 no.5_1
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    • pp.559-570
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    • 2017
  • A partial least squares regression (PLSR) model was developed to map the internal soluble solids content (SSC) of apples using a ground-based hyperspectral scanner that could simultaneously acquire outdoor data and capture images of large quantities of apples. We evaluated the applicability of various preprocessing techniques to construct an optimal prediction model and calculated the optimal band through a variable importance in projection (VIP)score. From the 515 bands of hyperspectral images extracted at wavelengths of 360-1019 nm, 70 reflectance spectra of apples were extracted, and the SSC ($^{\circ}Brix$) was measured using a digital photometer. The optimal prediction model wasselected considering the root-mean-square error of cross-validation (RMSECV), root-mean-square error of prediction (RMSEP) and coefficient of determination of prediction $r_p^2$. As a result, multiplicative scatter correction (MSC)-based preprocessing methods were better than others. For example, when a combination of MSC and standard normal variate (SNV) was used, RMSECV and RMSEP were the lowest at 0.8551 and 0.8561 and $r_c^2$ and $r_p^2$ were the highest at 0.8533 and 0.6546; wavelength ranges of 360-380, 546-690, 760, 915, 931-939, 942, 953, 971, 978, 981, 988, and 992-1019 nm were most influential for SSC determination. The PLSR model with the spectral value of the corresponding region confirmed that the RMSEP decreased to 0.6841 and $r_p^2$ increased to 0.7795 as compared to the values of the entire wavelength band. In this study, we confirmed the feasibility of using a hyperspectral scanner image obtained from outdoors for the SSC measurement of apples. These results indicate that the application of field data and sensors could possibly expand in the future.

Ecosystem Service Assessment of Urban Forest for Water Supply and Climate Mitigation of Seoul Metropolitan Area (환경공간정보를 이용한 수도권의 수자원 공급과 기후완화 기능을 위한 도시림의 생태계서비스 평가)

  • Lee, Soo Jeong;Yoo, Somin;Ham, Boyoung;Lim, Chul-Hee;Song, Cholho;Kim, Moonil;Kim, Sea Jin;Lee, Woo-Kyun
    • Korean Journal of Remote Sensing
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    • v.33 no.6_2
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    • pp.1119-1137
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    • 2017
  • This study assessed the water provisioning and climate mitigation ecosystem services of the urban forest in Seoul and Gyeonggi-do. The ecosystem service assessment is conducted based on natural function, natural function and population, and natural function and the beneficiary of the ecosystem service. Then, the impact of climate change on ecosystem services is analyzed to figure out the sensitivity of the impact on the beneficiary when the natural function of forest destroys under climate change. Gyeonggi-do has higher function-based water provisioning ecosystem service than Seoul. And population-based water provisioning ecosystem service appears to be higher in the densely populated area. On the other hand, beneficiary-based water provisioning ecosystem service by applying both natural water supply function and beneficiary distribution appears different with the result of population-based water provisioning service assessment. In other words, regions with high beneficiary population show higher ecosystem service than those with a low beneficiary population even though they have the same water storage function. In addition, climate change has a negative impact on the water provisioning ecosystem service. Under climate change, water provisioning service is expected to decrease by 26%. For climate mitigation service, regions close to the forest seem to have a low temperature, which indicates their high climate mitigation service. The center of the city with high beneficiary population shows high beneficiary-based ecosystem service. The climate change impacts the forest growth to decrease which affect the beneficiary-based climate mitigation ecosystem service to decrease by 33%. From this study, we conclude that beneficiary-based function and ecosystem service assessment is needed as well as the supply-based classification of forest function suggested by Korea Forest Service. In addition, we suggest that not only supply-based function classification and ecosystem service assessment but also beneficiary-based function classification and ecosystem service assessment is needed for managing the urban forest, which has been destroyed by climate change. This will contribute to revaluing cases where a forest with low natural function but high beneficiary-based ecosystem service, which is not considered under the current forest function-based assessment system. Moreover, this could assist in developing a suitable management plan for the urban forest.

Effect of Cover Crop Hairy Vetch on Prevention of Soil Erosion and Reduction of Nitrogen Fertilization in Sloped Upland (경사지 밭토양 유실억제 및 질소비료절감에 대한 피복작물 헤어리벳치의 효과)

  • Seo, Jong-Ho;Park, Jong-Yeol;Song, Duk-Young
    • Korean Journal of Soil Science and Fertilizer
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    • v.38 no.3
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    • pp.134-141
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    • 2005
  • Cultivation of winter cover crops and its soil utilization for summer main crops in slope upland is very important at the respects of diminution of soil erosion and non-point pollution source. The effects of cover crop, hairy vetch as no-tillage or conventional tillage on prevention of soil erosion and reduction of chemical nitrogen fertilization were investigated in the slope upland with whiter clover living mulch (partial tillage) in Suwon for three years and with rye (conventional tillage) in Hongcheon for two years, respectively. In Suwon, amounts of soil lost by rainfall runoff decreased as much as 90% by hairy vetch-no tillage (HV-NT), white clover-partial tillage (WF-PT) together with the decrease of rainfall runoff compared to winter fallow with conventional tillage (WF-CT). In addition, amounts of weed also decreased as much as 80-90% by HV-NT and WF-PT. Corn yield decreased much at the plot of WF-PT mainly due to competition for soil water and nutrients between clover and corn at the early corn growth stage. On the contrary, corn yield increased by HV-NT compared to WF-CT regardless of weed control. In Hongcheon, amounts of soil eroded during winter season before corn seeding were reduced as much as 95% by cultivation of hairy vetch and rye compared to winter fallow. Amount of soil eroded during waxy corn growing season was reduced as much as 98% by HV-NT compared to WF-CT. Also, soil incorporation of hairy vetch and rye as green manure with conventional tillage at corn seeding time could reduce soil erosion as much as 70% compared to no soil cover with conventional tillage. Ear yields of waxy corn were increased 10% higher at hairy vetch green manure (HV-CT) without nitrogen fertilizer, 20% higher at HV-NT with standard nitrogen fertilizer, respectively than WF-CT. But ear yields of waxy corn were decreased by rye green manure (R-CT) and HV-NT at the condition of no nitrogen fertilizer. It was concluded that hairy vetch was better as winter cover crop to reduce both soil erosion and chemical nitrogen fertilizer simultaneously in slope upland than other cover crops.

Studies on Amelioration of Soil Physico-Chemical Properties and Rice Yield in Sandy Tidal Saline Paddy Soil (사질(砂質) 염해답(鹽害畓)에서 개량제(改良劑) 시용(施用)이 토양(土壤)의 물리화학성(物理化學性) 변화(變化)와 수도수량(水稻收量)에 미친 영향(影響))

  • Yoo, Chul-Hyun;Kim, Jong-Gu;Choi, Song-Yeol;Cho, Guk-Hyun;Yoo, Sug-Jong;So, Jae-Dong;Rhee, Gyeong-Soo
    • Korean Journal of Soil Science and Fertilizer
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    • v.26 no.4
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    • pp.241-248
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    • 1993
  • An experiment was conducted in 1990~1991 to study the effects of various soil amelioration on the soil productivity and machine workability at tidal land paddy field of Kyewhado Substation, Homam Crop Experiment Station. The soil, Munpo Series(fine sandy loam, Typic Fluvaquents) was treated with gipsum, rice straw, rice straw compost and foreign soil(at 20cm depth) after deep ploughing. The results are surmerized as follows. 1. Sand and clay were slightly increased, while silt was slightly decreased in the rice straw and compost plots. The soil texture was changed from loam to sand loam by the addition of foreign soil 2. Soil bulk density and porosity was decreased in the rice straw, compost and foreign soil addition plots. 3. Cone penetration resistance was $12.5kg/cm^2$ at 10cm of soil depth before experiment and $12.5kg/cm^2$ at 20cm of soil depth after experiment except control, and the root zone was expended down to 20cm. 4. Soil salt content before experiment was 0.46 and 0.48% for surface soil(10cm) and subsoil(20cm), respectively ; The salt content of ameliorated plot was 0.26~0.32% and 0.16~0.31%, respectively, indicating good leaching of soil salt by the soil improvements. 5. The yields of rice in different treatments were in the order of the foreign soil addition > compost > gypsum > rice straw > control.

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Comparison Between Methods for Suitability Classification of Wild Edible Greens (산채류 재배적지 기준설정 방법 간의 비교 분석)

  • Hyun, Byung-Keun;Jung, Sug-Jae;Sonn, Yeon-Kyu;Park, Chan-Won;Zhang, Young-Seon;Song, Kwan-Cheol;Kim, Lee-Hyun;Choi, Eun-Young;Hong, Suk-Young;Kwon, Sun-Ik;Jang, Byoung-Choon
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.5
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    • pp.696-704
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    • 2010
  • The objective of this study was analysis of two methods of land suitability classification for wild edible green. One method was Maximum limiting factor method (MLFM) and the other was Multi-regression method (MRM) for land suitability classification for wild edible green. The investigation was carried out in Pyeongchang, Hongcheong, Hoeingseong, and Yanggu regions in Korea. The obtained results showed that factors related to the decision classification of the land suitability for wild edible green cultivation were land slope, altitude, soil morphology and gravel contents so on. The classification of the best suitability soil for wild edible greens were fine loamy (silty), valley or fan of soil morphology, well drainage class, B-slope (2~7%), available soil depth deeper than 100cm, and altitude higher than 501m. Contribution of soil that influence to crop yields using Multi-regression method were slope 0.30, altitude 0.22, soil morphology 0.13, drainage classes 0.09, available soil depth 0.07, and soil texture 0.01 orders. Using MLFM, area of best suitable land was 0.2%, suitable soil 15.0%, possible soil 16.7%, and low productive soil 68.0% in Hongcheon region of Gangwon province. But, area of best suitable land was 35.1%, suitable soil 30.7%, possible soil 10.3%, and low productive soil 23.9% by MRM. There was big difference of suitable soil area between two methods (MLFM and MRM). When decision classificatin of the land suitability for wild edible green cultivation should consider enough analysis methods. Furthermore, to establishment of land suitability classification for crop would be better use MRM than MLFM.

Studies on Early Seedling Establishment and Early Growth Responses of Astragalus membranaceus Bunge with Different Seeding times, Application Conditions, and Green Manure Crops for Developing Organic Agriculture Relating to Cropping System (작부체계 활용 유기재배기술 개발을 위한 녹비작물, 토양투입 조건 및 파종시기에 따른 황기의 입모율과 초기생육 특성 연구)

  • Song, Beom-Heon;Lee, Kyung-A;Chang, Yoon-Kee;Kim, Young-Gook;Ahn, Tae-Jin;Ahn, Young-Sup;Park, Chung-Beom
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.5
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    • pp.667-673
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    • 2010
  • Organic agriculture of especially medicinal crops has been interested and focused in taking the qualitative and safety agricultural products in order to maintain and improve the health and happiness of people. With respect to the organic agriculture relating to cropping system, objectives of this study were to examine the seedling establishment after seed germination and emergence and to investigate the early seedling growths of Astragalus membranaceus Bunge with treating two green manure crops, hairy vetch of legume and rye of gramineae, two different conditions of green manures, fresh and dry, and different seeding times. When the seeds were planted at once after treating the green manures with the fresh condition into the soil, the ratios of seedling establishment were very poor, less than 10% in hairy vetch and 20% in rye, respectively. They were increased gradually with delaying the seeding times after the treatments. With treating the green manures of dry conditions, the ratios of seedling establishment were up more than 50% in both hairy vetch and rye crops, showing that the damages of seedling establishment were more in hairy vetch than those in rye. The early seedling growths were slower in treating the green manures than those in the control, showing slow growth with fresh green manures compared to the dry. It would be very important and considered more to evaluate and utilize well the seed germination and the seedling establishment in cultivation of the organic agriculture using the green manure crops relating to the cropping system.

Changes in Fe, and Mn Content and Lime Requirement Based on Soil pH Testing in Sweet Persimmon Fields (단감 과원 토양 Fe, Mn 함량 변화와 pH 분석을 통한 석회소요량 추천)

  • Lee, Young-Han;Choi, Seong-Tae;Lee, Seong-Tae;Hong, Kang-Pyo;Song, Won-Doo;Lee, Jin-Ho;Cho, Ju-Sik
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.5
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    • pp.584-589
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    • 2010
  • Sweet persimmon yield can be limited by soil pH. This study was performed to rapidly determine the optimal level of lime requirement in sweet persimmon field. Soil chemical properties such as Fe, Mn, and Zn were analyzed soil samples of 31 sweet persimmon fields at Gyeongnam Province every two months from April to October in 2008. The available Fe, Mn, and Zn content were significantly high top soil (76.5 mg $kg^{-1}$ for Fe, 46.1 mg $kg^{-1}$ for Mn, and 16.9 mg $kg^{-1}$ for Zn, respectively), and subsoil (55.5 mg $kg^{-1}$ for Fe, 35.9 mg $kg^{-1}$ for Mn, and 12.3 mg $kg^{-1}$ for Zn, respectively) in April. Furthermore, the Mn content was significantly decreased since April compared to other micronutrients. We found a significant negative correlation between soil pH and lime requirement (r=0.881, $p{\leq}0.001$ for top soil, and r=0.855, $p{\leq}0.001$ for subsoil). We suggest that a conversion factor is -171 top soil pH + 1,148 to lime requirement (kg $10a^{-1}$) for top soil, and -190 subsoil pH + 1,247 to lime requirement (kg $10a^{-1}$) for subsoil in sweet persimmon fields. These results supported that the improvement of lime requirement by soil pH value is necessary to rapidly apply to field, and prevent micronutrients over uptake by persimmon plant.

Evaluation of Green House Gases Emissions According to Changes of Soil Water Content, Soil Temperature and Mineral N with Different Soil Texture in Pepper Cultivation (고추재배에서 토성별 토양수분, 토양온도, 무기태 질소 변화에 따른 온실가스배출 평가)

  • Kim, Gun-Yeob;Song, Beom-Heon;Roh, Kee-An;Hong, Suk-Young;Ko, Byung-Gu;Shim, Kyo-Moon;So, Kyu-ho
    • Korean Journal of Soil Science and Fertilizer
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    • v.41 no.6
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    • pp.399-407
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    • 2008
  • Importance of climate change and its impact on agriculture and environment has increased with a rise of greenhouse gases (GHGs) concentration in Earth's atmosphere, which had caused an increase of temperature in Earth. Greenhouse gas emissions such as methane($CH_4$) and nitrous oxide($N_2O$) in the field need to be assessed. GHGs fluxes using chamber systems in the fields(2004~2005) with pepper cultivation were monitored at the experimental plots of National Academy of Agricultural Science(NAAS), Rural Development Administration(RDA) located in Suwon city. $N_2O$ emission during pepper growing period was reduced to 74.0~82.1% in sandy loam soil compared with those in clay loam soil. Evaluating $N_2O$ emission at different levels of soil water conditions, $N_2O$ emission at -50 kPa were lowered to 13.2% in clay loam soil and 40.2% in sandy loam soil compared with those at -30 kPa. $CH_4$ emission was reduced to 45.7~61.6% in sandy loam soil compared with those in clay loam soil. Evaluating $CH_4$ at different levels of soil water conditions, $CH_4$ emission at -50 kPa was lowered to 69.6% in clay loam soil and 55.8% in sandy loam soil compared with those at -30 kPa. It implied that -50 kPa of soil water potential was effective for saving water and reducing GHG emissions. From the path analysis as to contribution factors for $N_2O$ emission, it appeared that contribution rate was in the order of mineral N(51.2%), soil temperature (25.8%), and soil moisture content(23.0%) in clay loam soil and soil moisture content(39.3%), soil temperature (36.4%), and mineral N(24.3%) in sandy loam soil.

Evaluation of CO2 Emission to Changes of Soil Water Content, Soil Temperature and Mineral N with Different Soil Texture in Pepper Cultivation (고추재배에서 토성별 토양수분, 토양온도, 무기태질소 변화에 따른 CO2 배출량 평가)

  • Kim, Gun-Yeob;Song, Beom-Heon;Hong, Suk-Young;Ko, Byong-Gu;Roh, Kee-An;Shim, Kyo-Moon;Zhang, Yong-Seon
    • Korean Journal of Soil Science and Fertilizer
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    • v.41 no.6
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    • pp.393-398
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    • 2008
  • Several researchers have proposed models or equations to predict soil $CO_2$ flux from more readily available biotic and abiotic measurement. Tree commonly used abiotic variables were N mineral and soil temperature and soil water content. This study was conducted to determine $CO_2$ emission to mineral N, soil water content and soil temperature with clay loam and sandy loam in pepper cultivation in 2004~2005. $CO_2$ flux in the upland with different levels of soil water potential was measured at least once in two weeks during the cropping period in the pepper cultivation plots. Soil water potential in the clay loam and sandy loam soils was established at -30kPa and -50kPa by measuring the soil gravimetric water content with two replications. $CO_2$ emission rate from the differently managed plots was highly correlation coefficient to between the mineral N ($R=0.830^{**}$, $0.876^{**}$) and soil temperature ($r^2=0.793^{**}$, $0.804^{**}$) in the clay loam and sandy loam, respectively. However, the relationships between $CO_2$ emission and soil water content were non-significant. $CO_2$ emissions at sandy loam soils was lower to 21~37% than at clay loam soils for both soil water conditions without differences in yield. At difference levels of soil water conditions, $CO_2$ emission at -50kPa decreased to 37.5% in comparison with that at -30kPa. From the path analysis as to contribution factors of GHGs, it appeared that contribution rate was in the order of soil temperature (54.9%), mineral N (32.7%), and soil moisture content (12.4%).