• Journal of Plant Biotechnology
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• v.7 no.4
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• pp.241-246
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• 2005

Factors affecting regeneration of different Rubus varieties (blackberry, raspberry and their hybrid) were examined and a reliable regeneration system was established. Media for stock plant maintenance were tested; different explants and media were investigated to find the best circumstances for the regeneration. The effect of the commonly used antibiotics was studied to determine the most suitable one for selection of the transformants. We found that both MS and LS media supplemented by $20\;gL^{-1}$ sucrose are suitable for the stock plant maintenance. The optimal hormone content for the stock plants is $0.125\;mgL^{-1}$ 6-benzylaminopurine (BAP) with $0.01\;mgL^{-1}$ indole-3- butyric acid (IBA). The highest regeneration rate was observed on medium containing MS salts with B5 vitamins complemented with glucose, sucrose, maltose, $10\;gL^{-1}$ each, supplemented with benzylaminopurine riboside (BAR) ($2\;mgL^{-1}$) and indole-3-acetic acid (IAA) ($0.1\;mgL^{-1}$). The regenerated shoots appeared directly from the cut edges, without callus phase. Hygromycin and geneticin proved to be good selection agents for the Rubus explants, but due to their severe effect on the tissues we propose to use marker-free constructions for the transformation.

• Natural Product Sciences
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• v.15 no.3
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• pp.156-161
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• 2009

Rubus coreanus, commonly known as 'red raspberry' is used as a traditional oriental medicine in Korea for the management of diseases such as impotence, spermatorrhea and athsma, and for allergies, in combination with other herbal preparations, in many centuries. We undertook a comparison of the hepatoprotective effect of ethanol extracts of the unripe (UREx) and ripe (RREx) R. coreanus extract against acetaminophen (AAP) induced hepatotoxicity in rats. UREx reduced the elevated alanine aminotransferase (ALP), aspartate aminotransferase (AST), total bilirubin (TB), alkaline phosphatase (AP), lipid peroxide and nitric oxide content which had been increased by AAP administration. UREx also increased the cellular glutathione (GSH) content and induced the glutathione-S-transferase (GST), glutathione peroxidase (GSH-Px) content which had been decreased by AAP. RREx did not exhibit strong hepatoprotective effect or antioxidant activity under the same conditions. The experimental results show that the degree of the ripening of R. coreanus affects the hepatoprotective activity in the AAP-intoxicated rats. These findings of a protective mechanism are supportive evidence for the utility of unripened R. coreanus in traditional medicine for liver ailments.

• Food Science of Animal Resources
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• v.33 no.3
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• pp.377-389
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• 2013

Gamma-amino butyric acid (GABA) is a kind of pharmacological and biological component and its application is wide and useful in Korea specially, becoming aging society in the near feature. GABA is request special dose for the purposed biological effect but the production of concentrated GABA is very difficult due to low concentration of glutamic acid existed in the fermentation broth. To increase GABA concentrate using fermentation technology, high content of glutamic acid is required. For this reason, various strains which have the glutamic acid decarboxylase (GAD) and can convert glutamic acid to GABA, were isolated from various fermented foods. Most of GABA producing strains are lactic acid bacteria isolated from kimchi, especially added monosodium glutamate (MSG) as a taste enhancer. Optimizing the formulation of culture media and the culture condition, GABA conversion yield and amounts were increased. Finally GABA concentration of fermentation broth in batch or fed batch fermentation reached 660 mM or 1000 mM, respectively. Furthermore formulation of culture media for GABA production developed commercially. Many studies about GABA-rich product have been continued, so GABA-rich kimchi, cheese, yogurt, black raspberry juice and tomato juices has been also developed. In Korea many biological effects of GABA are evaluated recently and GABA will be expected to be used in multipurpose.

• Food Science and Biotechnology
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• v.16 no.2
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• pp.270-274
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• 2007

To characterize the polysaccharides which exist as soluble forms in fruit wines, crude polysaccharides were isolated from red, white, raspberry, wild grape, and pear wine, respectively. Among them, the crude polysaccharide (RW-0) in red wine showed the highest yield and considerable amounts of thiobarbituric acid (TBA)-positive materials. The pectic polysaccharide RW-2 was purified to homogeneity from RW-0 by subsequent size-exclusion chromatography using Sephadex G-75 and its structure was characterized. RW-2 consisted of 14 different monosaccharides which included rarely observed sugars in general polysaccharides, such as 2-O-methyl-fucose, 2-O-methyl-xylose, apiose (Api), 3-C-carboxy-5-deoxy-L-xylose (aceric acid, AceA), 3-deoxy-D-manno-2-octulosonic acid (Kdo), and 3-deoxy-D-lyxo-2-heptulosaric acid (Dha). Methylation analysis indicated that RW-2 comprised at least 20 different glycosyl linkages such as 3,4-linked fucose, 2,3,4-linked rhamnose, 3'-linked apiose, and 2,3,3'-linked apiose, being characteristic in rhamnogalacturonan II (RG-II). High performance size-exclusion chromatography indicated that RW-2 mainly comprised RG-II of higher molecular weight (12,000), and that the changes of molecular weight to apparent 7,000 under less than pH 2.0 were observed. These analyses indicated that the higher molecular weight polysaccharide in RW-2 was mainly present as a RG-II dimer.

• Atmosphere
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• v.28 no.3
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• pp.317-324
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• 2018

In this study, we review the ground-based whole-sky camera (WSC), which is developed to continuously capture day and night cloud images using the illumination data from a precision Lightmeter with a high temporal resolution. The WSC is combined with a precision Lightmeter developed in IYA (International Year of Astronomy) for analysis of an artificial light pollution at night and a DSLR camera equipped with a fish-eye lens widely applied in observational astronomy. The WSC is designed to adjust the shutter speed and ISO of the equipped camera according to illumination data in order to stably capture cloud images. And Raspberry Pi is applied to control automatically the related process of taking cloud and sky images every minute under various conditions depending on illumination data from Lightmeter for 24 hours. In addition, it is utilized to post-process and store the cloud images and to upload the data to web page in real time. Finally, we check the technical possibility of the method to observe the cloud distribution (cover, type, height) quantitatively and objectively by the optical system, through analysis of the captured cloud images from the developed device.

• Journal of Electrochemical Science and Technology
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• v.8 no.1
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• pp.25-34
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• 2017

Concurrent electrocatalysis and sensing of hydrazine, sulfite ions, and nitrite ions in a mixture were studied using electrodes modified by electrodeposited Au nanostructures (NSs). The ${\beta}$-cyclodextrin-mixed silicate sol-gel composite was drop-casted on the electrode surface and nucleation guided by ${\beta}$-cyclodextrin occurred, followed by the electrodeposition of Au NSs. The additive, ${\beta}$-cyclodextrin, played an evident role as a structure-directing agent; thus, small raspberry-like Au NSs were obtained. The modified electrodes were characterized by surface characterization techniques and electrochemical methods. The Au NSs-modified electrodes effciently electrocatalyzed the oxidation of toxic molecules such as hydrazine and sulfite and nitrite ions even in the absence of any other electron transfer mediator or enzyme immobilization. Well-resolved oxidation peaks along with decreased overpotentials were noticed during the electrooxidation process. The fabricated Au nanostructured electrode clearly distinguished the electrooxidation peaks of each of the three analytes from their mixture.

• Journal of IKEEE
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• v.21 no.3
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• pp.227-233
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• 2017

Python language is widely used because of its ease of learning and its wide application range. Arduino, on the other hand, is also widely utilized hardware for physical computing and internet of things(IoT). However, Arduino is controlled by $C{^+^+}$ language, which makes it difficult for non-experts to enter swiftly. This paper proposes an errorless protocol that can simultaneously control multiple Arduino devices in a master device using python language with I2C communication. Using the protocol proposed in this paper, we can take advantage of the python language to control multiple Arduinos.

• Journal of the Korea Society of Computer and Information
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• v.24 no.3
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• pp.77-86
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• 2019

The advent of Internet of Things (IoT) makes common nowadays computing environments involving programming not a single computer but several heterogeneous distributed computers together. Developing programs separately, one for each computer, increases programmer burden and testing all the programs become more complex. To address the challenge, this paper proposes an RPC-based unified programming language, UniPy, for development of MGC (eMbedded, Gateway, and Cloud) applications in IoT systems configured with popular computers such as Arduino, Raspberry Pi, and Web-based DB server. UniPy offers programmers a view of classes as locations and a very simple form of remote procedure call mechanism. Our UniPy compiler automatically splits a UniPy program into small pieces of the program at different locations supporting the necessary RPC mechanism. An advantage of UniPy programs is to permit programmers to write local codes the same as for a single computer requiring no extra knowledge due to having unified programming models, which is very different from the existing research works such as Fabryq and Ravel. Also, the structure of UniPy programs allows programmers to test them by directly executing them before splitting, which is a feature that has never been emphasized yet.

• Journal of the Korea Society of Computer and Information
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• v.24 no.6
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• pp.109-115
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• 2019

Smart Factory is a part of and a key point of the 4th industrial revolution. It performs optimization from the whole viewpoint, using comprehensive data of the post-process data by utilizing various sensors, controllers, and mobile devices beyond the existing factory automation level. In this paper, we design and implement an IoT platform that can detect the safety factors of the workers, the environmental factors of the factory, and real time monitoring at the control center, among the fields to implement smart factory. To accomplish this, we construct a monitoring device that provides sensor information control, server transmission of sensor information, and visualization of collected information. By using this system, it is possible to maintain the temperature and humidity for the optimum working environment in the factory. and also, By using the beacon, it is possible to measure the working time of the worker and trace the position.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2016.10a
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• pp.593-595
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• 2016

In recent, the cafe culture has came very close friendly people. But many customers have been left the seat because the cafe has been heavily biased. To solve the problem, we propose the Cafe Seat Confirmation System based on IoT. Proposed system consists of Arduino, Raspberry Pi, and application. Using a smart device, it is possible to provide efficient services such as real-time seat reservations and cafes management.