The theory of island biogeography has influenced strongly on the conservation and management issues of species diversity in the islands. The theory has not limited to islands in the ocean, but expanded to isolated and fragmented areas in the forests and urban. This study has a rare opportunity to explore the species diversity and abundance of birds in the islands of Korea. A total of 151 islands in the west and south areas in the Korean peninsula were examined. The number of species and the area of islands were highly significant (P<0.000) and the number of species and the distance from the mainland showed not so strong relationship. This indicated that bird species diversity has more influenced by the size of the islands not the distance. This can be from the study species of birds that can fly long distance, and also natural characteristics of migratory and resident status. Species-area curve showed that the z-value was 0.21 indicating the area and the bird species are strongly correlated. The long-term monitoring of bird species presence in the islands should be followed for a proper management plan.
The aim of this study was to explore the accuracy and feasibility of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) in identifying bacteria from environmental sources, as compared with rpoA gene sequencing, and to evaluate the occurrence of bacteria of the genus Enterococcus in wild birds. In addition, a phyloproteomic analysis of certain Enterococcus species with spectral relationships was performed. The enterococci were isolated from 25 species of wild birds in central Europe (Poland). Proteomic (MALDI-TOF MS) and genomic (rpoA gene sequencing) methods were used to identify all the isolates. Using MALDI-TOF MS, all 54 (100%) isolates were identified as Enterococcus spp. Among these, 51 (94.4%) isolates were identified to the species level (log(score) ${\geq}2.0$), and three isolates (5.6%) were identified at a level of probable genus identification (log(score) 1.88-1.927). Phylogenetic analysis based on rpoA sequences confirmed that all enterococci had been correctly identified. Enterococcus faecalis was the most prevalent enterococcal species (50%) and Enterococcus faecium (33.3%) the second most frequent species, followed by Enterococcus hirae (9.3%), Enterococcus durans (3.7%), and Enterococcus casseliflavus (3.7%). The phyloproteomic analysis of the spectral profiles of the isolates showed that MALDI-TOF MS is able to differentiate among similar species of the genus Enterococcus.
Amir Hossein Alizadeh-Ghamsari;Amir Reza Shaviklo;Seyyed Abdullah Hosseini
Journal of Animal Science and Technology
/
v.65
no.4
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pp.804-817
/
2023
This study was conducted to evaluate the effects of co-dried fish protein hydrolysate (CFPH) on broilers performance, intestinal microbiology, and cellular immune responses. Five hundred one-day-old (Ross 308) male broilers were allocated to four treatments with five replicates of 25 birds in a completely randomized design. The experimental treatments included four levels of CFPH (0% as the control, 2.5%, 5%, and 7.5%) in the isonitrogenous and isocaloric diets. During the experiment, body weight (BW) and feed intake (FI) were periodically recorded in addition to calculating average daily gain (ADG), feed conversion ratio (FCR), liveability index, and European broiler index (EBI). In addition, cellular immune responses were evaluated at 30 days of age. On day 42, ileal contents were obtained to examine the microbial population. Based on the findings, Dietary supplementation of 5 and 7.5% CFPH increased the percentage of the thigh while decreasing the relative weight of the gizzard compared to the control group. The highest relative length of jejunum was observed in birds receiving 2.5 and 5% CFPH, and its highest relative weight belonged to birds fed with 5% CFPH. The number of coliforms, enterobacters, and total gram-negative bacteria in the intestines of birds receiving CFPH was less than that of the control group. In general, the application of CFPH in broiler nutrition can decrease the level of soybean meal in diet and it can be considered as a new protein supplement in poultry production. It is suggested to study the incorporation of this new supplement in other livestock's diets.
Alexandra L. Wealleans;Roba Abo Ashour;Majdi A. Abu Ishmais;Sadiq Al-Amaireh;David Gonzalez-Sanchez
Journal of Animal Science and Technology
/
v.66
no.3
/
pp.457-470
/
2024
This study aimed to evaluate the effect of supplementing different protease enzymes on growth performance, intestinal morphology, and selected carcass traits in broilers fed diets reduced 3.5% in crude protein (CP) and amino acids (AA). One thousand one-day-old Ross 308 broilers (41 g) were assigned to five dietary treatments with ten replicates of 20 birds each: a positive control (PC) diet formulated to meet Ross 308 AA requirements, a negative control (NC) diet reformulated to provide 3.5% lower CP and AA compared to PC, NC supplemented with a multi-protease (PR1) solution, containing 3 different coated proteases produced from Aspergillus niger, Bacillus subtilis and Bacillus licheniformis, NC supplemented with a serine protease (PR2) produced from Bacillus licheniformis, and NC supplemented with an alkaline protease (PR3) produced from Bacillus licheniformis. At slaughter, 40 birds per treatment were used to assess the effect of the different treatments on carcass traits. At 32 days, samples of the duodenum, jejunum, and ileum of 10 birds per treatment were collected for intestinal morphology evaluation. Birds fed PC and NC supplemented with multi-protease exhibited better (p < 0.05) feed efficiency compared to NC and NC supplemented with all the other protease enzymes. Multi-protease supplementation was linked to the highest (p < 0.05) carcass weight and yield. There were significant differences (p < 0.05) between treatments in all gut segments, with PC, PR1, PR2, and PR3 exhibiting longer villi height (VH) compared to NC. This study demonstrates that 3.5% reduction of CP and AA negatively affected for the overall period feed efficiency, carcass yield, and intestinal morphology. The supplementation of the multi-protease restored feed efficiency and improved carcass yield.
For the purpose of contributing to wild birds protection and propagation, distribution characteristics and ecological breeding habitats were surveyed in Mt. Chiri from September, 1986 to December, 1989 by the use of the nestboxes installed in four study sites by the altitude. The results were as follows ; 1. During the study period, observed birds were 2,403 individuals belong to 59 species, especially, at the 1,400m above altitude the rare species, Falco tinnunculus and the protecting bird species such as Ninox scutulata, Strix aluco and Prunella collaris were observed. 2. In the inhabitation structure of birds by the altitude, Fringilla montfringilla was the highest dominance (41.33%) among the inhabitated birds in the surveyed area. 3. Phasianus colchicus and Streptopelia orientalis which were mainly inhabited in hilly area were observed until the altitude of 900m. 4. The 92.5% of nest boxes was mainly used by the small birds, Parus spp. and Sitta europaea. These species preferred the nest boxes installed at the edge or along the mountaineering path, because they could get food and water easily from those places. 5. The utilization rate of the nest boxes was showed 86% at 500m, 96% at 900m, 98% at 1,400m and 90% at 1,700m above the altitude, respectively. 6. The breeding rate of birds in the nest boxes was showed from 6% to 46% by altitude. Parus varius was the highest breeding species in the surveyed area. These birds inhabited above 500m laid eggs from 15th, April to 22th, May(n=22), but those above 1,700m laid eggs from 28th, April to 27th, May(n=13), that 13 days were delayed at high altitude. 7. Food items of the chicks of the 5 species collected by the collar method were animal matters ; 91.3% of the food was forest insects such as Phalera asiimilis and Pleuroptya batteata except 8.7% of spiders.
In order to observe an effect of the components of rice straw on the utilization of nutrient in chicks, the rice straw of 100g were digested in 800$m\ell$ of distilled water or 0.25N NaClO$_2$ at 135 C and in the pressure of 3.2kg/$\textrm{cm}^2$ by autoclave during 30, 60 and 120 minutes (water or NaClO$_2$-30, 60 and 120- RS). The contents of neutral detergent fiber(NDF), acid detergent fiber (ADF) and lignin were analysed in the washed and dried rice straw meal. Hatched single comb white Leghorn male chicks were fed with a commercial chick mash for the first 10 days and five kinds of experimetal diets for the next 8 days which contained 17.0% of wheat bran (basal), cellulose(cotton meal), nontreated RS, water-30-RS and NaClO$_2$ 30-RS, respectively. The water-30, 60 and 120-RS baa leased 9.7, 12.1 and 13.3% of dry matter, respectively, while NaClO$_2$-30-RS had similar contents of dry matter loss with those of water-30-RS, and NaClO$_2$-60 and 120-RS had tossed 1.5 times of dry matter comparing with those of water-60 and 120-RS, respectively. And the dry matter loss of the water-RS or NaClO$_2$-RS was mainly originated front the extractable cell contents and hemicellulose of the non-treated RS. Birds fed water-30-RS diets had higher body weight gain and lower feed conversion than those of birds fed non-treated and NaClO$_2$-30-RS diets during 8 days of experimental feeding. Also nitrogen balance and retention rate of birds fed water -30-RS was higher comparing with those of birds non-treated and NaClO$_2$-30-RS. And digestibility of crude fat had been shown a highering trend in birds water-30-RS. The rate of metabolizable energy (MEn) to gross energy (GE) of birds fed non-treated RS, water-30-RS and NaClO$_2$30-RS diets were 71.9, 72.9 and 70.4%, respectively, and energy intake per metaboic body size (kg 0.75) were reached to 307.3, 296.2 and 291.4 kcal per day, respectively. And daily protein retention per kg 0.75 were 1.647, 1.969 and 1.560g, respectively. Then 30.56kcal of MEn required for 1 g of protein retention in birds fed water-30-RS, which was lower thu 36.90 and 37.56 kcal of birds fed non-treated and NaClO$_2$-30- RS, respectively. The results seems to indicate that non-treated rice straw had a substance or characters which affect the energy unilization or protein retention of diets and which will be eliminated by boiling in water.
Newcastle disease virus (NDV) infects a variety of birds with a wide range of clinical signs from asymptomatic to severe. During a 10-month period in 2011, a total of 1,024 sera from wild birds including 42 species of birds in 8 orders were collected and the seroprevalence of NDV in wild birds was evaluated by hemagglutination inhibition (HI) test. Evidence of NDV infection was observed in 12.6% (129/1,024) of wild birds with a maximum prevalence reported in Mandarin duck (27.8%, 32/115) followed by Mallard duck (20.8%, 57/274), Spot-billed duck (11.9%, 36/303), Pintail (2.9%, 1/34), Black-tailed gull (2.9%, 1/34), White-fronted goose (1.8%, 1/56) and Common teal (1.4%, 1/69). None of the other 35 species of wild birds were antibody-positive for NDV. Mandarin duck, Mallard duck and Spot-billed duck showed high sero-prevalance of 12.2% to 42% during winter season (November to March). Our results indicate that Mandarin duck, Mallard duck and Spot-billed duck might be natural reservoirs for NDV in Korea and the prevalence of NDV infection in wild birds displayed a seasonal pattern with high prevalence of NDV in winter season (November to March).
This study is to identify potential factors to impact on appearance of wildbirds which live in the core forest around urban area in Gangdong-gu, Seoul. As a result of analysis of, studies on biotope showed most of urbanization biotope was biotope of residential areas with high green coverage and biotope of residential and business areas with low green coverage while most of biotope of green and openspace was core green biotope. The research area was divided into several blocks based on biotope types in the urbanization areas excluding green and openspace. As a result of research on wildbirds, total 51 species 3,419 individuals appeared in spring and total 35 species 4,213 individuals appeared in winter. 24 wild bird species were selected as subjects of this study among 31 species seen in urbanization areas, since urban birds, rapacious birds, waterside birds were excluded from the study for the proper consideration. Then this study looked at how many species and individuals of the subjects were observed at each research block in urbanization areas during spring and winter separately. Landuse structure and green structure in each block were examined to see whether these structures affect the number of wild birds observed in urbanization areas of Gangdong-gu. Furthermore, the distance between these blocks and green was assessed. While studying the potential links between the landuse structure and the number of wild birds observed in urbanization areas of Gangdong-gu, block area, green coverage, and building-to-land ratio were believed to affect the number of types and species of wild birds in the research area. In terms of correlation analysis of whether green structure affected the number of wild birds observed in urbanization areas of Gangdong-gu, crown volume of layers, the average green patch area, the average height of canopy layer were found to have an impact not only on the number of types but also species of wild birds in the research area.
Kim, In-Kyu;Lee, Han-Soo;Paek, Woon-Kee;Lee, Joon-Woo
Korean Journal of Environment and Ecology
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v.24
no.2
/
pp.147-156
/
2010
This study was conducted from April, 2002 to March 2006, using three urban streams(Gap Stream, Yudeung Stream and Daejeon Stream) in Daejeon Metropolitan City. 12,027 individual birds summed by the peak count in 126 species, 34 families, and 13 orders were observed from three stream sites. Dominant species were of Anas poecilorhyncha, Anas crecca, Columba livia, Passer montanus, and Egretta garzetta(in that order). The groups of birds were classified into six types. The most frequent group were the arbor birds(54 species), while the smallest group was the diving ducks(7 species). As for the number of individuals, the shrub bird group had 721 individuals while the dabbling ducks observed had 4,974 individuals. Regarding the distribution of birds appeareing in each stream, 14,885 individual numbers in 114 species were observed at Gap Stream, 6,642 individuals in 90 species at Yudeung Stream and 4,202 individuals in 69 species at Daejeon Stream. Various indices of the birds were analyzed with respects to the similarities between streams. Gap Stream had similar characteristics to Yudeung Stream, and the latter was similar to Daejeon Stream. However, Gap Stream and Daejeon Stream showed different characteristics. The dominance index of each section was calculated using ten dominant bird species top-down. Subsequently, the birds and their preferred environment were analyzed. The results showed that shrub birds and arbor birds preferred the upper stream of every stream, while herons and dabbling ducks inhabited the midstream. Dabbling ducks and some diving ducks appeared downstream.
Two broiler experiments were carried out to determine whether supplementation of zeolite in the diet would affect broiler performance and economical efficiency. Day old male broiler chickens of Abor Acres strain were used. In the first experiment,60 birds were distributed into f groups of 10 birds each. Three groups of chickens were randomly placed on one of the following single-stage (0-6wks) experimental diets; control diet (non-supplemented) or 2% zeolite supplemented diet (supplemented to the control). In the second experiment,216 birds were distributed into 24 groups of 9 birds each. Eight groups of chickens were randomly placed on one of the 3 two-stage diet (starter;0-4wks, finisher;5-7wks);control diet ($T_1$ , non-supplemented), 2% zeolite diet ($T_2$:isocaloric and isonitrogenous as $T_1$ ) and 2% zeolite supplemented diet ($T_3$, supplemented to the control). Birds were housed in the batteries, and feeds and water were fed ad libitum. The results of Experiment 1 showed that birds fed control diet were heavier than those fed 2% zeolite supplemented diet up to 4th wks of age. However, birds fed the latter grew more at 5th and 6th wk. Overall weight gain, feed intake and feed efficiency were not significantly different. The results of Experiment 2 showed that there were no significant differences in weight gains, feed intakes and feed efficiencies among treatments during starter, finisher to overall period. The moisture levels in excreta of the birds were significantly (P<0.01) less when zeolite was included in the diets and those in excreta significantly (P < 0.01) increased as the ambient temperature increased. Economical efficiency expressed in fed cost (won) required per kg weight gin were 346.55 (T$_3$),348.34 ($T_1$ ) and 359.85 ($T_2$).
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