• 한국축산시설환경학회지
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• 제5권3호
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• pp.157-170
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• 1999

This study was conducted to investigate the effects of Scoria, Quartz porphyry and Oak charcoal powder feeding by 1% level of concentrate on the fattening cross bred bulls(5/8 Korean Native Cattle, 2/8 Charolais, 1/8 Brahman germ plasma) Feeding trial was conducted with 4 treatment groups which were T1(Full feeding of concentrate and roughage for 12 months), T2(T1 and Scoria addition), T3(T1 and Quartz porphyry addition) and T4(Oak charcoal addition) for 360 days, consisting of 32 heads whose initially weights were about 299.7kg. The results obtained are summarized as follows; 1. During the over-all period, average daily gains by T3, T4, T2 and T1 were 1.024, 0.987, 0.977kg and 0.964, respectively, without a significant difference by treatments. 2. Concentrate intakes per body weight and TDN intakes required per unit of kilogram gain were lower in addition agent groups than in control, and in all the treatments feed efficiency was higher at early stage for 360 fattening day than at end stage. 3. Carcass weight, dressing percent, back-fat thickness and loin eye muscle area by treatments were 330.9 to 348.4kg(average 340.3kg), 55.2~56.2(average 55.8%), 0.41~0.55(average 0.46cm)and 76.0~80.9$\textrm{cm}^2$(average 77.6$\textrm{cm}^2$), respectively, and were improved in addition agent groups than in control. 4. Meat quality grade is higher in addition agent groups than in control, and heads of 1st grade by treatments was 3, 2, 1 and none in T4, T2, T3 and T1, respectively. 5. Incidence heads of diseases by treatment were not apparently different, but diseases of digestive system was decreased in addition agent groups than in control. 6. The nitrate nitrogen content of fattening bull dung which collected in rectum were 0.082~0.089% (average 0.084%), and the content in addition agent groups was smaller than in controll. 7. Income was higher in order of T4, T3, T2, and T1 and incomes of treatment groups were grater than that of control group by 21.4 to 33.5 percent. According to the above results it may be concluded that fattening bulls may be required to feed no more than 1% of Scoria, Quartz porphyry and Oak charcoal powder based on the concentrate feeding level during the fattening period(12 to 24 month of age) to produce high quality meat and increase income.

• 한국식품위생안전성학회지
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• 제22권4호
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• pp.346-352
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• 2007

2006년에서 2007년까지 국내에서 생산된 사료 중 585점(배합사료 447, 단미사료 138)의 사료에서 아플라톡신 오염도를 조사하였다. 단미사료 중 $AFB_1$과 $AFB_2$의 오염도는 각각 20%와 3%이었으며, 오염농도는 각각 $0.48{\sim}10.46ppb$와 0.25-0,42 ppb로 나타났다. 배합사료에서는 $AFB_1$ 오염도가 38%로 나타났으며, 평균 0.43-5.52 ppb 수준의 검출농도로 분석되었다. 그리고 조사된 사료 중 2%의 배합사료가 0.26-0.46 ppb의 농도로 $AFB_2$에 오염되어 있는 것으로 나타났다. 사료의 종류별로 아플라톡신 오염을 비교시 고기소 사료(75%)> 젖소 사료(72%)> 강피류(30%)순으로 $AFB_1$이 많이 오염되어 있는 것으로 나타났으며, $AFB_1$ 평균오염농도는 강피류(3.1 ppb)에서 가장 높았으며, 박류(2.9 ppb), 개사료(1.9 ppb) 순으로 감소되는 것으로 나타났다. 검사한 모든 시료에서 $AFG_1$와 $AFG_2$는 검출되지 않았다.

• Reproductive and Developmental Biology
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• 제38권3호
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• pp.129-136
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• 2014

본 시험은 무항생제 사육방식이 한우육내 코티졸과 지방산 함량에 미치는 영향을 구명하고자 실시하였다. 무항생제 및 관행 축산으로 생산된 한우육 1등급 등심(M. longissimus)부위를 3가지의 브랜드별로 각각 3회씩 구매하여 시료로 공시하였고, 분석한 결과는 다음과 같다. 한우육 등심내 코티졸 함량은 무항생제 처리구에서 관행사육 처리구 대비 유의적(p=0.0176)으로 낮게 나타났으며, 포화지방산 및 불포화지방산 함량에서는 유의적(p>0.05)인 차이를 나타내지는 않았으나, 관행사육 처리구에서는 포화지방산이, 무항생제 처리구에서는 불포화지방산이 각각 높은 경향으로 분석되었다. 또한, 불포화지방산 중 n-3과 n-9 지방산을 제외한 n-6 지방산(linoleic acid, ${\gamma}$-linolenic acid)에서 유의적(p<0.01)인 차이를 보였다. 또한, 한우육내 코티졸 농도와 n-6 지방산 농도간의 상관관계를 분석한 결과, 특히 linoleic acid와 유의적(p=0.0140)으로 부(-)의 상관관계가 성립되었다. 결과적으로, 무항생제 인증으로 사육된 한우육은 관행적으로 사육된 한우육에서 보다 상대적으로 코티졸 함량이 낮았으며, 건강에 유익한 불포화지방산 함량이 다소 높은 경향으로 분석됨으로써 육질을 향상시키는 것으로 나타났다.

• Journal of Animal Science and Technology
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• 제44권5호
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• pp.607-616
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• 2002

계절별로 도축된 한우육의 육색에 미치는 요인들을 구체적으로 분석하고자 2000년도에 도축된 한우의 pH, 성별, 등지방두께, 등심단면적, 도체중, 출하연령, 도체심부온도, 외기온도, 외기상대습도 요인이 육색에 미치는 영향을 분석하였다. 분석결과, 여름에는 L*값(명도)이 비거세우가 유의적으로 낮았던 것을 제외하고 모든 육색특성에서 유의적이지 않은 것으로 나타났으며 기타 계절에서는 모든 육색특성에서 거세우가 유의적으로 높은 값을 나타내었다. 또한 중회귀분석결과 모든 육색특성(L*, a*, C*, $h^0$)에서 pH가 증가할수록 그 값들은 감소하였으며, 등지방두께는 증가할수록 그 값들은 증가하였다. 각 요인별로 의사결정나무분석을 실시한 결과, 명도(L*)가 가장 높은 것은 봄과 가을에 도축한 암소와 거세우였으며 적색도(a*)는 가을에 도축한 한우중 pH가 5.63 미만이고 외기상대습도가 71.5% 이상인 경우인 것으로 나타났다. Chroma값(C*)은 여름과 가을에 도축하고 pH가 5.60 미만이면서 등지방두께가 8mm 이상인 한우가 가장 높았으며, hue angle($h^0$)은 봄, 여름, 가을에 도축하고 pH가 5.66 미만이며 등지방두께가 8mm 이상인 한우 중 심부온도가 4.7$^{\circ}C$ 미만인 것으로 나타났다.

• 한국초지조사료학회지
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• 제36권2호
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• pp.104-108
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• 2016

본 실험은 동일 사료원료 조건에서 농후사료와 조사료를 혼합 급여하는 섬유질배합사료(TMR)와 분리급여 방식이 메탄발생량에 미치는 효과를 조사하기 위하여 홀스타인 거세 숫소 6두(평균체중 $203{\pm}24.8kg$)를 공시하여 changeover design으로 실험을 수행하였다. TMR구는 육성기용 배합사료, 티모시 건초 그리고 비지를 생중량 기준 각각 40, 48, 12% 비율로 매일 혼합 급여하였고, 분리급여구는 배합사료와 비지만을 급여 전 혼합하여 대사틀에 설치된 사료통에 먼저 넣고 그 위에 티모시를 올려주었다. 사료급여량은 공시축이 전량 섭취할 수 있도록 약 0.2 kg의 일당증체 수준으로 제한 급여하였기 때문에 처리구간 차이가 없었고, 메탄발생량(g/d)에서도 유의적인 차이가 없었다(P>0.1). 그러나 메탄 전변율(Ym, 메탄 에너지/GE intake)은 TMR구가 분리급여구 보다 유의적으로 높았다(p=0.05). 두 처리 평균 메탄 배출계수(kg $CH_4$/head/year)는 21.4였고, 평균 메탄 전변율은 0.05이었으며, 공시축의 대사체중(metabolic BW, $kg^{0.75}$)과 메탄발생량(g/d)과는 높은 상관관계($R^2=0.8057$, p<0.001)를 보였다. 반추가축의 대표적인 사료급여방식 차이가 메탄발생량에 미치는 효과에 대한 결론을 얻기 위해서는 보다 많은 연구가 필요할 것으로 사료된다.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1246-1246
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• 2001

The quality of meat is highly variable in many properties. This variability originates from both animal production and meat processing. At the pre-slaughter stage, animal factors such as breed, sex, age contribute to this variability. Environmental factors include feeding, rearing, transport and conditions just before slaughter (Hildrum et al., 1995). Meat can be presented in a variety of forms, each offering different opportunities for adulteration and contamination. This has imposed great pressure on the food manufacturing industry to guarantee the safety of meat. Tissue and muscle speciation of flesh foods, as well as speciation of animal derived by-products fed to all classes of domestic animals, are now perhaps the most important uncertainty which the food industry must resolve to allay consumer concern. Recently, there is a demand for rapid and low cost methods of direct quality measurements in both food and food ingredients (including high performance liquid chromatography (HPLC), thin layer chromatography (TLC), enzymatic and inmunological tests (e.g. ELISA test) and physical tests) to establish their authenticity and hence guarantee the quality of products manufactured for consumers (Holland et al., 1998). The use of Near Infrared Reflectance Spectroscopy (NIRS) for the rapid, precise and non-destructive analysis of a wide range of organic materials has been comprehensively documented (Osborne et at., 1993). Most of the established methods have involved the development of NIRS calibrations for the quantitative prediction of composition in meat (Ben-Gera and Norris, 1968; Lanza, 1983; Clark and Short, 1994). This was a rational strategy to pursue during the initial stages of its application, given the type of equipment available, the state of development of the emerging discipline of chemometrics and the overwhelming commercial interest in solving such problems (Downey, 1994). One of the advantages of NIRS technology is not only to assess chemical structures through the analysis of the molecular bonds in the near infrared spectrum, but also to build an optical model characteristic of the sample which behaves like the “finger print” of the sample. This opens the possibility of using spectra to determine complex attributes of organic structures, which are related to molecular chromophores, organoleptic scores and sensory characteristics (Hildrum et al., 1994, 1995; Park et al., 1998). In addition, the application of statistical packages like principal component or discriminant analysis provides the possibility to understand the optical properties of the sample and make a classification without the chemical information. The objectives of this present work were: (1) to examine two methods of sample presentation to the instrument (intact and minced) and (2) to explore the use of principal component analysis (PCA) and Soft Independent Modelling of class Analogy (SIMCA) to classify muscles by quality attributes. Seventy-eight (n: 78) beef muscles (m. longissimus dorsi) from Hereford breed of cattle were used. The samples were scanned in a NIRS monochromator instrument (NIR Systems 6500, Silver Spring, MD, USA) in reflectance mode (log 1/R). Both intact and minced presentation to the instrument were explored. Qualitative analysis of optical information through PCA and SIMCA analysis showed differences in muscles resulting from two different feeding systems.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 2017년도 춘계공동학술대회
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• pp.23-23
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• 2017

Recent favor on high quality food and concern on food safety have demonstrated the superiority of Hanwoo (Korean native cattle). In general, the price of cow is higher than those of steer and bull, causing cheating issues in the market. Hence, this study is to discriminate genders of Hanwoo with identification of factors which highly influence gender discrimination based on the big-size deboning data. Totally, there were 31 variables in the deboning data, and we divided into them two categories: data obtained before and after deboning. Discriminant function analysis was then applied into the data to determined the accuracy of gender discrimination in Hanwoo. The result showed that Hanwoo could be classified by gender with 99.2% of accuracy when using all 31 variables. In detail, it was possible to identify 93 of 94 bulls (98.9%), 96 of 96 cows (100%) and 74 of 75 steers (98.7%). The most significant variables was chuck, sirloin, armbone shin, plates, retail and cuts percentage, sequentially. With variables obtainable before deboning, accuracies of classification were 91.5% for bulls, 92.7% for cows, and 89.3% for steers. The most significant variables was water, cold carcass weight and back-fat thickness. The discrimination accuracy was higher with data obtainable after deboning: bulls (98.9%), cows (99.0%) and steers (98.7%). In this case, chuck, sirloin and armbone shin were the factors determined the classification ability. This study showed that Hanwoo can be classified based on deboning data with appropriate statistics, further suggesting weight of cut of beef might be the standard for gender classification.

• 한국축산식품학회지
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• 제34권3호
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• pp.339-345
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• 2014

This study was performed to develop a rapid immuno-assay kit, by using a specific antigen to detect Hanwoo brand meat. We selected a synthetic antigen specific to our target antibody, named BIO-TAG (Tyr-D-Ala-Phe), by utilizing a computer-based analysis and literature review. BIO-TAG tagged with adjuvant was subcutaneously injected in sheep and Hanwoo. The serum and meat juice of the immunized or non-immunized animal were then analyzed, to measure the titer of antibody by ELISA and Western blot. The amount of antibodies against the BIO-TAG increased (p<0.05) in serum by vaccination. Furthermore, meat juice from the immunized Hanwoo showed greater (p<0.05) antibody titer, compared with those from non-immunized groups. To optimze the dilution factor, we performed dot-ELISA, with various combination levels of BIO-TAG. Results from dot-ELISA showed that 2 mg/mL BIO-TAG was sufficient to distinguish the immunized meat from non-immunized groups. These results support our hypothesis that simple immunization of Hanwoo generates a sufficient amount of antibodies to be detectable in the meat juice by means of the immune-assay. Therefore, specific Hanwoo brand meat can be more precisely identified by our rapid diagnostic kit. This technology can deter possible fraud of counterfeit meat brands in the Korean domestic market with ease and rapidity; and offers a new tool that guarantees consumers high quality Hanwoo brand beef.

• 농업과학연구
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• 제48권4호
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• pp.831-842
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• 2021

Accurate measurements of feed energy values have a positive effect on the control of feed intake for animals, the formation of prices, and on economic profits. However, few studies have attempted to measure the energy values of domestic roughages and to validate an energy value prediction model. The purpose of this study is to build a database through measurements of the nutrient digestibility and digestible energy results of roughages used in Korea and to evaluate the domestic applicability of the National Research Council (NRC) estimation model. Oat hay, annual ryegrass, tall fescue, barnyard millet, and concentrates for beef cattle were used in the test. As a result of an in vivo digestibility trial, the total digestible nutrients (TDN) of oat hay, annual ryegrass, tall fescue, and barnyard millet were found to be 68.73, 55.02, 55.71, and 52.89%, respectively. As a result of comparing the estimated values using the NRC equation with the in vivo results, there was a difference of 3.55 to 6.84%P in the TDN. Inferred from this result, it is considered that TDN calculations using the NRC equation can be reasonable for the test feeds used in this study. These results can be utilized when revising the Korean Standard Tables of Feed Composition, which provides a comprehensive overview of Korean feed.

• 한국축산식품학회지
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• 제22권3호
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• pp.218-221
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• 2002

수입생우인 Hereford, Angus, Murray Grey, Cross breed (Hereford$\times$Angus)의 채끝을 채취하여 도축 후 0$\pm$1$^{\circ}C$에서 7일간 숙성 후 이화학적 특성을 비교하였다. 수입우 품종별 일반성분 분석결과 수분은 교잡종이 71.8%로 가장 높았으며, Hurray Grey종이 69.1%로 가장 낮았다 (p<0.05). 단백질과 지방은 품종간 유의적인 차이를 보이지 않았으나, 회분은 Hurray Grey종이 가장 높았고, Angus종이 가장 낮았다(p<0.05). 물리적 특성 중 pH와 지방경도는 품종간 유의적인 차이를 보이지 않았으나(p>0.05), 보수성은 Hurray Grey종이 가장 높았고, 전단력은 Angus종이 가장 높았으며, Hereford와 Murray Grey종이 가장 낮았다(p<0.05). 가열감량은 Murray Grey종이 가장 낮았으며, 나머지 품종들 간에는 유의적인 차이가 없었다(p>0.05). 수입우의 육색 및 관능검사 결과는 품종간에 유의적인 차이를 보이지 않았다 (p>0.05). 본 연구결과 수입우 품종간 일부 조사항목을 제외하고는 육질에 큰 차이를 보이지 않았다. 따라서 국내 수입 생우의 국내비육 후 소비자의 선호도 등, 보다 폭 넓은 연구가 필요하며, 아울러 한우와 동일한 사육프로그램에 의해 사육하여 한우와 수입 생우간 육량 및 육질에 대하여 포괄적인 비교연구가 필요하다 하겠다.