• Title/Summary/Keyword: Korea Science Academy

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Identification of An Antibacterial Gene by Differential Display from Lipopolysaccharide-Stimulated Dung Beetle, Copris tripartitus

  • Suh, Hwa-Jin;Kim, Yeon-Ju;Bang, Hea-Son;Yun, Eun-Young;Kim, Seong-Ryul;Park, Kwan-Ho;Kang, Bo-Ram;Kim, Ik-Soo;Jeon, Jae-Pil;Hwang, Jae-Sam
    • International Journal of Industrial Entomology and Biomaterials
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    • v.17 no.2
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    • pp.223-228
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    • 2008
  • A novel beetle antimicrobial protein from stimulated Copris tripartitus and the corresponding gene were isolated in parallel through differential display-PCR and expression in Escherichia coli. To find cDNA clones responsible for bacteria resistance, the suppression subtractive hybridization and GeneFishing differentially expressed genes system were employed in the dung beetle, Copris tripartitus immunized with lipopolysaccaride. One cDNA clone from eight subtracted clones was selected through dot blot analysis and confirmed by northern blot analysis. The 516-bp, selected cDNA clone was determined by 5' and 3' rapid amplication of cDNA ends and cloned into the GST fusion expression vector pGEX-4T-1 for expression of the protein. The expressed protein was predicted 14.7 kDa and inhibited the growth of gram-negative bacteria such as Escherichia coli and Pseudomonas aeruginosa. These results implied that the expressed protein is related to immune defense mechanism against microorganism.

Mulberry popcorn disease occurrence in Korea region and development of integrative control method

  • Ju, Wan-Taek;Kim, Hyun-Bok;Sung, Gyoo-Byung;Park, Kwang-Young;Kim, Yong-Soon
    • International Journal of Industrial Entomology and Biomaterials
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    • v.33 no.1
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    • pp.36-40
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    • 2016
  • Mulberry fruits also have tremendous potential for providing various valuable industrial products of very high economic value for human beings. Nevertheless, through global warming, the popcorn disease caused by sclerotia forming fungi reduces the productivity of mulberry fruits in worldwide. So, in this study, we investigated damage ratio of mulberry popcorn disease in mulberry fruit production farm (Buan, Jeongueb, Sangju, Gochang in Korea). In Jeonbuk Buan, popcorn disease rate was the highest about 30%, on the other hand, in case of Gyungbuk Sangju and Jeonbuk Gochang, not damage. Also, we investigated about popcorn disease prevention by various of chemical treatment methods.

Leaf Spot of Yam Caused by Pseudophloeosporella dioscoreae in Korea

  • Hong, Sung-Kee;Kim, Wan-Gyu;Lee, Young-Kee;Choi, Hyo-Won;Choi, Kyung-Jin;Lee, Sang-Yeob
    • Mycobiology
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    • v.38 no.1
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    • pp.78-80
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    • 2010
  • Leaf spot symptoms were frequently observed on yam plants grown in the Yeoju area in Korea during a disease survey in 2008. A total of five isolates of Pseudophloeosporella sp. were obtained from the infected leaves of yam plants. All of the isolates were identified as Pseudophloeosporella dioscoreae based on their morphological and cultural characteristics. A phylogenetic tree derived from the internal transcribed spacer sequences of the fungal isolates showed that the fungus is distinctly separated from species in other related genera. P. dioscoreae isolates caused very tiny spots on leaves of yam plants two weeks after artificial inoculation which were similar to those observed in the field. This is the first report that Pseudophloeosporella dioscoreae causes leaf spot in yams in Korea.

A Newly Recorded Genus and Species in Korea, Malthacus angusticollis (Motschulsky) (Cantharidae, Coleoptera)

  • Kang, Tae-Hwa;Lee, Young-Bo;Hwang, Seok-Jo;Park, Hae-Chul
    • International Journal of Industrial Entomology and Biomaterials
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    • v.21 no.2
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    • pp.193-195
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    • 2010
  • Both the genus Malthacus Kirby and M. angusticollis (Motschulsky) are reported for the first time in Korea. Five North Korean specimens of M. angusticollis were loaned from Hungarian Natural History Museum, Budapest, Hungary and Sungshin Women's University, Seoul, Korea. Here we provide a key to the genus of the Podabrini in Korea and a diagnosis and taxonomic account of the genus Malthacus and M. angusticollis.

Establishment of Soil Suitability for Korean Black Raspberry by Soil Morphological and Physical Properties (토양의 형태 및 물리적 특성을 고려한 복분자 재배적지 기준설정)

  • Hyun, Byung-Keun;Cho, Hyun-Jun;Sonn, Yeon-Kyu;Park, Chan-Won;Chun, Hyen-Chung;Song, Kwan-Cheol;Moon, Yong-Hee;Noh, Dae-Cheol;Yun, Kwan-Hee;Kim, Myung-Sook;Lee, Deog-Bae
    • Korean Journal of Soil Science and Fertilizer
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    • v.46 no.2
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    • pp.92-98
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    • 2013
  • The objective of this study was to establish the decision criteria of soil suitability for Korean Black raspberry using soil morphological and physical properties. The investigation was carried out in Gochang, Sunchang, Jeongeup, Pohang, and Hoengseong districts in Korea. The obtained results showed that factors related to the decision criteria of the soil suitability for Korean Black raspberry cultivation were soil texture, soil drainage class, land slope, and available soil depth. The criteria of the best suitability soil for Korean Black raspberry was valley/fan or hill geomorphology, well or moderately drainage class, B-slope(2-7%), coarse loamy soil texture family, less than <15% gravel contents, and available soil depth deeper than 100cm. The area criteria of soil suitability for Korean Black raspberry of Gochang was more than 50% including best and suitable areas.