• Animal Systematics, Evolution and Diversity
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• 제35권1호
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• pp.1-5
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• 2019

The specimens of caddisflies previously recorded as Sortosa distincta (Walker, 1852), Rhyacophila uchidai Kobayashi, 1989, Rhyacophila jirisana Kobayashi, 1989, Crunobiodes koriaensis Kobayashi, 1989, and Ganonema odaenum Kobayashi, 1989 from South Korea were reexamined. Rhyacophila uchidai and R. jirisana are newly synonymized with R. retracta Martynov, 1914 and R. vicina Botosaneanu, 1970, respectively. We reconfirm two synonyms, C. koriaensis with Lepidostoma sinuatum (Martynov, 1935) and G. odaenum with Psilotreta locumtenens Botosaneanu, 1970, and a misidentification of Dolophilodes affinis Levanidova and Arefina, 1996 as S. distincta. Some specimens recorded as G. odaenum are identified as Psilotreta falcula Botosaneanu, 1970.

• Asian Journal of Atmospheric Environment
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• 제5권3호
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• pp.172-180
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• 2011

The transfer of microorganisms is important process for ecosystems. Microorganisms in dryland can transport itself to wetland through atmospheric diffusion, but only few papers reported about the atmospheric bioaerosol present over dryland. We carried out the direct sampling using a tethered balloon over Dunhuang City, China's northwestern dryland. Bioaerosols were collected using a tethered balloon with a bioaerosol collector at 820 m above the ground (1,960 m above the sea level) around noon on August 17, 2007. The bioaerosols were cultured after the collection at Dunhuang Meteorological observatory. Two strains of molds were isolated using the Nutrient agar medium. About 400-bp 18S rRNA partial sequences were amplified by PCR and determined afterwards. The results of a homology search by 18S rRNA sequences of isolates in DNA databases (GenBank, DDBJ, and EMBL) and an observation of the form revealed that two bioaerosols in the convective mixed layer over Dunhuang City were Cladosporium sp. and Aspergillus sp.

• Asian-Australasian Journal of Animal Sciences
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• 제17권3호
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• pp.423-427
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• 2004

Seventy-six animals including cattle, sheep, horses, 6 species of zoo animals were employed for collection of fresh feces in order to detect verotoxigenic Esherichia coli (VTEC) by safe, quick and sensitive PCR-based molecular methods. Bacterial cell disruption with bead-beating followed by bacterial DNA purification with hydroxyapatide chromatography and gel filtration allowed DNA preparation from animal feces with high recovery and purity. A mountain goat was firstly shown by PCR and sequencing to shed verotoxin 2 gene (vt2) that was used to generate vt2 probe and second primer set for nested PCR to attempt more sensitive detection. Most sensitive nested PCR revealed that 45% of tested cattle and 47% of tested zoo animals were VTEC-positive, while least sensitive normal PCR detected VTEC from none of these animals except a mountain goat. Moderately sensitive detection by PCR in combination with hybridization suggested that the VTEC density varied between the VTEC-positive cattle.

• Asian Journal of Atmospheric Environment
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• 제5권3호
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• pp.164-171
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• 2011

This work focuses on the analysis of bioaerosols in the atmosphere at higher altitudes over Noto Peninsula, Japan. We carried out direct sampling via aircraft, separated cultures, and identified present isolates. Atmospheric bioaerosols at higher altitudes were collected using a Cessna 404 aircraft for an hour at an altitude of 3,500 m over the Noto Peninsula. The aircraft-based direct sampling system was devised to improve upon the system of balloon-based sampling. In order to examine pre-existing microorganism contamination on the surface of the aircraft body, bioaerosol sampling was carried out just before takeoff using the same method as atmospheric sampling. Identification was carried out by a homology search for 16S or 18S rDNA isolate sequences in DNA databases (GenBank). Isolate sampling just before takeoff revealed Stretpomyces sp., Micrococcus sp., and Cladosporium sp. One additional strain, Bacillus sp., was isolated from the sample after bioaerosol collection at high altitude. As the microorganism contamination on the aircraft body before takeoff differed from that while in the air, the presence of additional, higher atmosphere-based microorganisms was confirmed. It was found that Bacillus sp. was floating at an altitude of 3,500 m over Noto Peninsula.