• Journal of Microbiology and Biotechnology
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• v.16 no.7
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• pp.1149-1153
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• 2006

In this study, nuclease-resistant RNA aptamers that are specific for Jurkat T leukemia cells were selected by a subtractive systemic evolution of ligands by exponential enrichment (SELEX) method. A randomized nuclease-resistant RNA library was incubated with normal peripheral blood mononuclear cells (PBMC) in each round to preclude RNAs that recognize the common cellular components on the surface of normal and cancer cells. The precluded RNAs were used for the selection of Jurkat T cell-specific aptamers, and the specific RNAs were then gradually enriched from start to the following selections. After 16 rounds of the subtractive SELEX, the selected aptamers were found to preferentially bind to Jurkat T cells, but not to the normal PBMC, evidenced by fluorescence-activated cell sorting analysis. Thus, the subtractive SELEX can be used to identify ligands to cancer-specific biological markers without prior knowledge of the nature of markers. The aptamers could be applied to specific cell sorting, tumor therapy, and diagnosis, and moreover, to find cancer cell-specific markers.

• The Transactions of the Korea Information Processing Society
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• v.5 no.1
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• pp.11-23
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• 1998

In very large databases, the problem of searching for interesting information effectively is very important in terms of efficiency and flexibility. A multiple layered database approach based on AOG(attribute-oriented generalization) method is one of the useful approaches for knowledge discovery under various situations. In this paper, we propose a multiple layered database design methodology based on AOG method in object-oriented databases. In addition, we propose a dynamic schema evolution model and implementation strategy in order to continue providing information effectively in multiple layered databases.

• Animal Systematics, Evolution and Diversity
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• v.32 no.1
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• pp.21-27
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• 2016

The solitary wasp genus Phimenes (Hymenoptera: Vespidae: Eumeninae) was reported to occur in Vietnam by , represented by one taxon, Phimenes flavopictus continentalis (Zimmermann), which was synonymized under nominotypical Phimenes flavopictus by . A note on gender of this genus is made in the text. One more species, Phimenes indosinensis is recorded in this study from Dak Lak in the southern and Son La in the northwestern parts of Vietnam for the first time. Detailed descriptions of the female and male of the latter are provided with figures. A key to the two species from Vietnam is also provided.

• Animal Systematics, Evolution and Diversity
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• v.30 no.1
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• pp.26-32
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• 2014

Cleantioides planicauda (Benedict, 1899) has previously been recorded in China and Japan. In the present study, to our knowledge, we report for the first time the occurrence of this species in South Korea. Here, we provide detailed descriptions and illustrations of the diagnostic characteristics of C. planicauda. Further, for the first time, we determined the partial sequence of mitochondrial cytochrome c oxidase subunit 1 (CO1) gene of C. planicauda for molecular characteristic. Additionally, we provide a key to the Korean species and distribution of the genus Cleantioides. As a result of this study, four species of the genus Cleantioides, including C. planicauda, have now been recorded in South Korea.

• Journal of Korean Academy of Nursing
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• v.24 no.4
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• pp.616-622
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• 1994

Precise concept analysis has been neglected be-cause of a lack of understanding of its necessity and a lack of conceptual analytic knowledge. Concept analysis is the mental work of examining parts, phenomenon and the interrelated whole of a thing. Focus in this article is to extract the critical attributes of hardiness and make an operational delinition. The process of concept analysis is illustrated and documented using the analytic approach described by Walker and Avant (1983). To explore the explicit or implicit meanings of hardiness, existing literature was reviewed. The evolution of hardiness and the dictionary definitions were also added. Hardiness can be defined as follows : A condition of being inured to fatigue or hardship which has three subcomponents-challenge, control and commitment. Critical attributes of hardiness were extracted that may be used in naming the occurrence of the phenomenon. Model case, contrary case, borderline case and related case were described. Antecedents and consequences were explored. The defining critical attributes of hardiness are : 1. Resistance-hardiness involves resistance of stress or hardship. 2. Hardiness involves appraisal of change as a chance. 3. Hardiness implies interpretation of events and self as influential,. 4. Hardiness requires active involving reaction Implications for nursing and for further study are added.

• Journal of Ecology and Environment
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• v.38 no.3
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• pp.361-366
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• 2015

We investigated cladoceran fauna from 71 study areas in South Korea. Sixty-two taxa were found, of which there were 30 planktonic and 25 littoral-benthic cladoceran species. In the present study, taxa of cladocera were identified by biographical traits. Species endemic to the Far East were found in the northernmost areas of South Korea, whereas Palearctic species were found in the southernmost areas. Generally, coexistence of cladoceran species in northern and southern areas was observed, implying possible faunistic complexes of cladocera in various areas of South Korea. We believe that further studies conducted in different habitats will greatly expand our knowledge of the biodiversity of cladocera in South Korea.

• Journal of The Korean Astronomical Society
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• v.29 no.spc1
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• pp.35-38
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• 1996

Much of our knowledge about the formation and evolution of high-redshift galaxies has come from studying the absorption signatures they impress on the spectra of background QSOs. The damped Lyman $\alpha$ (DLA) systems, in particular, have proved to be valuable probes of the metallicity and dust at redshifts z $\~$2-3 in what are the likely progenitors of galaxies like our own. At z $\~$ 2 we find that the typical metallicity of the universe was 1/15 solar. In addition, we find clear evidence for the existence of trace amounts of interstellar dust in DLA galaxies and show that this is consistent with recent high resolution spectra of DLAs with the Keck telescope, despite claims to the contrary.

• Journal of Institute of Control, Robotics and Systems
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• v.4 no.5
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• pp.624-629
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• 1998

Evolutionary computation techniques can solve search problems using simulated evolution based on the ‘survival of the fittest’. Recently, the genetic programming (GP) which evolves computer programs using the genetic algorithm was introduced. In this paper, the genetic programming technique is used in order to design a rule based controller consisting of condition-action rules for an unknown system. No a priori knowledge about the structure of the controller is needed. Representation of a solution, functions and terminals in GP are analyzed, and a method of constructing a fuzzy logic controller using the obtained rule based controller is described. A simulation example using a nonlinear system shows the validity and efficiency of the proposed method.

• Journal of the Korean Operations Research and Management Science Society
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• v.37 no.4
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• pp.19-35
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• 2012

The Semantic Web technology, purporting to share, to reuse and to process by machines data stored in the Web environment, incessantly evolves to help human decision making; in particular, decision making based on data, or quantitative decision making. This trend drives researchers to fill the gap with strenuous efforts between the current state of the technology and the terminus of this evolution. The Semantic Web Constraint Language (SWCL) together with SWRL is one of these endeavors to achieve the goal. This paper focuses particularly on how to express the piecewise linear form in the context of SWCL. The importance of this ingredient can be fortified by the fact that any nonlinear expression can be approximated in the piecewise linear form. This paper will also provide the information of how it will work in the decision making process through an example of the Internet shopping mall problem.

• Proceedings of the Technology Innovation Conference
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• 1999.06a
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• pp.176-202
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• 1999

Korea has exerted her efforts to assimilate nuclear power technology, and reportedly localized 95 percent of nuclear power technology by 1995. This paper investigates the evolution of nuclear power program in Korea to exploit the development process of the nuclear power industry and key factors for the technological localization of nuclear power plant. In developing countries, an imitative catching-up process can be shown as a course for developing the absorptive capacity of foreign technology, which depends on prior knowledge base and the intensity of effort. The process of technological learning consists of five stages including preparation, implementation of foreign technology, acquisition of peripheral technology, acquisition of core technology, and improvement f foreign technology. Moreover, this paper discusses six essential factors that have influenced the successful achievement of technological localization of nuclear power plants in Korea. They include the role and strategies of the government, the leading role of utility firm, the development and cooperation of the related organizations, the development of human resources and their efforts, market conditions and the assistance of foreign donors, and social conditions. Finally, this paper discusses about implications offered by the Korean experience for other developing countries.