• Parasites, Hosts and Diseases
• /
• v.57 no.3
• /
• pp.283-290
• /
• 2019

A rapid diagnostic test (RDT) kit was developed to detect non-structural protein 1 (NS1) of yellow fever virus (YFV) using monoclonal antibody. NS1 protein was purified from the cultured YFV and used to immunize mice. Monoclonal antibody to NS1 was selected and conjugated with colloidal gold to produce the YFV NS1 RDT kit. The YFV RDTs were evaluated for sensitivity and specificity using positive and negative samples of monkeys from Brazil and negative human blood samples from Korea. Among monoclonal antibodies, clones 3A11 and 3B7 proved most sensitive, and used for YFV RDT kit. Diagnostic accuracy of YFV RDT was fairly high; Sensitivity was 0.0% and specificity was 100% against Dengue viruses type 2 and 3, Zika, Chikungunya and Mayaro viruses. This YFV RDT kit could be employed as a test of choice for point-of-care diagnosis and large scale surveys of YFV infection under clinical or field conditions in endemic areas and on the globe.

• Parasites, Hosts and Diseases
• /
• v.49 no.3
• /
• pp.207-212
• /
• 2011

Rapid serodiagnostic methods for Toxoplasma gondii infection in cats are urgently needed for effective control of transmission routes toward human infections. In this work, 4 recombinant T. gondii antigens (SAG1, SAG2, GRA3, and GRA6) were produced and tested for the development of rapid diagnostic test (RDT). The proteins were expressed in Escherichia coli, affinity-purified, and applied onto the nitrocellulose membrane of the test strip. The recombinant SAG1 (rSAG1) showed the strongest antigenic activity and highest specificity among them. We also performed clinical evaluation of the rSAG1-loaded RDT in 182 cat sera (55 household and 127 stray cats). The kit showed 0.88 of kappa value comparing with a commercialized ELISA kit, which indicated a significant correlation between rSAG1-loaded RDT and the ELISA kit. The overall sensitivity and specificity of the RDT were 100% (23/23) and 99.4% (158/159), respectively. The rSAG1-loaded RDT is rapid, easy to use, and highly accurate. Thus, it would be a suitable diagnostic tool for rapid detection of antibodies in T. gondii-infected cats under field conditions.

• Proceedings of KOSOMES biannual meeting
• /
• 2018.06a
• /
• pp.4-4
• /
• 2018

• Journal of the Korean Society of Manufacturing Process Engineers
• /
• v.14 no.6
• /
• pp.90-97
• /
• 2015

Optimization of multi-quality characteristics in injection molded parts is very important, but it is sometimes difficult for part/mold designers. The objective of this study is to develop a practical design methodology for optimizing two-quality characteristics of injection molded parts. To attain this end, we developed a new design-range reduction algorithm based on Taguchi's orthogonal arrays for two characteristics. Then, the algorithm was integrated with commercial injection-molding simulation tools. A feature of the proposed methodology is that it allows field-designers unfamiliar with general optimization methods to be able to apply the methodology to their design problems with ease. Finally, we have applied the proposed design methodology to optimization of weldlines and deflections in an actual bezel model. The results show the usefulness of this methodology.

• Korean Journal of Veterinary Research
• /
• v.40 no.3
• /
• pp.601-609
• /
• 2000

An enzyme linked immunosorbent assay (ELISA) was developed to screen residues of sulfadimethoxine (SDM) in edible animal products. An indirect competitive ELISA was allowed to compete with rabbit anti-SDM for binding to a limited amount of SDM-gelatin conjugate and SDM in serum samples. Sera was diluted 20 times with phosphate buffered saline (PBS) and boiled for 5 minutes to destruct immunoglobulins of serum. Detection limit of this competitive ELISA for SDM was 0.1 ppb or less. Among eight sulfonamide analogues tested for specifity, only sulfamonomethoxine showed significant cross-reaction in the assay. The EC-50 value for sulfamonomethoxine was 3.5 ppm. Recovery of SDM in spiked serum samples between 100 ppb and 500 ppb ranged from 110.7% to 128.9%.

• Transactions of the Korean Society of Mechanical Engineers B
• /
• v.32 no.1
• /
• pp.62-69
• /
• 2008

A post-processing program based on the OOP(Object-Oriented Programming) concept has been developed for flow visualization of the flow analysis code(PowerCFD) using unstructured cell-centered method. User-friendly GUI(GTaphic User Interface) has been built on the base of MFC(Microsoft Foundation Class). The program is organized as modules by classes including those based on VTK(Visualization ToolKit)-library, and these classes are made to function through inheritance and cooperation which is an important and valuable OOP concept. The major functions of this post-processor program are introduced and demonstrated, which include mesh plot, contour plot, vector plot, surface plots, cut plot, clip plot, xy-plot and streamline plot as well as view manipulation (translation, rotation, scaling etc).

• Transactions of the Korean Society of Mechanical Engineers B
• /
• v.32 no.1
• /
• pp.70-77
• /
• 2008

A pre-processing program based on the OOP(object-oriented programming) concept has been developed. The program consists of the input of a 2D or 3D flow problem to a CFD program by means of an user-friendly interface and the subsequent transformation of this input into a form suitable for the solver(PowerCFD) using unstructured cell-centered method. User-friendly GUI(graphic user interface) has been built on the base of MFC(Microsoft Foundation Class). The program is organized as modules by classes based on VTK(Visualization ToolKit)-library, and these classes are made to function through inheritance and cooperation which is an important and valuable concept of object-oriented programming. The major functions of this program are introduced and demonstrated, which include mesh generation, boundary settings, solver settings, generation of grid connectivity and geometric data etc.

• Journal of the Korean Society of Industry Convergence
• /
• v.10 no.4
• /
• pp.235-240
• /
• 2007

Special quality of automobile CMC(clutch master cylinder) and analyzed aluminum, plastic material comparison. Efficiency of plastic master cylinder can modularize higher, light weight anger of parts, several piece parts by single parts, prove NVH(Noise, Vibration, Harshness) than aluminum master cylinder as analysis result. Also, structure is easy simplicity, exchange, maintenance costs can be reduced and decrease environmental pollution because recycling is superior.

• Proceedings of the Korean Society of Computer Information Conference
• /
• 2014.01a
• /
• pp.45-47
• /
• 2014

본 논문에서는 증강현실 기술을 응용하여 한정된 공간에서가 아닌 실제 세상에서 사람들과 상호작용할 수 있는 모바일 응용 프로그램 개발 전략을 클라이언트와 서버 단으로 나누어 설명하였다. ARToolKit이라는 오픈소스 라이브러리를 사용하여 증강현실을 구현하였으며, 클라이언트 시스템은 동시에 다수의 사용자가 상호작용을 하는 것을 이슈로 구현하였고, 서버 시스템은 클라이언트의 요청에 대한 신속한 응답처리를 위해 처리 과정에서의 의사 결정 단계를 최소화 시킨다는 것을 목표로 구현하였다.

• Proceedings of the KIPE Conference
• /
• 2017.07a
• /
• pp.196-197
• /
• 2017

최근 국내 태양광산업은 보급량이 증가하고 있고, 정부 정책에서도 에너지신산업을 추진하는 등 시장 활성화에 노력하고 있다. 본교에서는 신재생에너지원 중 태양광발전시스템에 대해 건학이념인 실사구시에 입각한 학부 교육 프로그램을 개발 및 적용하고 있으며 본 논문에서는 이에 대한 내용을 소개한다. 산업체 실무에 기초한 태양광발전시스템 학부 교육 프로그램은 마이크로콘트롤러(MCU) 교육, DC-DC 컨버터와 DC-AC 인버터 등 전력변환장치 기반의 Solar Explorer Kit 교육, 태양광발전시스템 발전량 산출과 손실분석 등 성능평가를 위한 PVsyst 엔지니어링 교육 내용으로 구성된다. 특히, 전력변환장치로 구성된 Solar Explorer Kit 교육 프로그램은 DC-DC 컨버터와 배터리(리튬-폴리머) 충전, 배터리(리튬-폴리머) 방전과 DC LED 부하 접속, 독립형 DC-AC 인버터 출력과 AC 할로겐 램프 부하 접속, 계통연계형 DC-AC 인버터 출력 실습 등에 대해 소개한다.