• Journal of Microbiology and Biotechnology
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• 제25권12호
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• pp.2049-2057
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• 2015

Various salt concentrations (1.0%, 1.3%, 1.6%, 1.9%, and 2.1% labeled as sample A, B, C, D, and E, respectively) were investigated for microbial diversity, identification of Lactic Acid Bacteria (LAB) in salted kimchi cabbage, prepared under laboratory conditions. These samples were stored at 4°C for 5 weeks in proper aluminum-metalized pouch packaging with calcium hydroxide gas absorber. A culture-independent method known as polymerase chain reaction - denaturing gradient gel electrophoresis was carried out to identify LAB distributions among various salt concentration samples that had identified 2 Weissella (W. confusa and W. soli), 1 Lactobacillus (Lb. sakei), and 3 Leuconostoc (Lc. mesenteroides, Lc. lactis, and Lc. gelidum) in the overall kimchi samples. The pH, titratable acidity, viable cell counts, and coliform counts were not affected by salt variations. In order to assess sensory acceptance, the conducted sensory evaluation using a 9-point hedonic scale had revealed that samples with 1.3% salt concentration (lower than the manufacturer's regular salt concentration) was more preferred, indicating that the use of 1.3% salt concentration was acceptable in normal kimchi fermentation for its quality and safety. Despite similarities in pH, titratable acidity, viable cell counts, coliform counts, and LAB distributions among the various salt concentrations of kimchi samples, the sample with 1.3% salt concentration was shown to be the most preferred, indicating that this salt concentration was suitable in kimchi production in order to reduce salt intake through kimchi consumptions.

• 한국식생활문화학회지
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• 제9권1호
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• pp.87-93
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• 1994

This study was conducted to present a method that determine the optimum conditions for the preparation of chinese whole cabbage kimchi. After Sensory and chemical characteristics of kimchi with various salt concentration in brine, brining time and storage period at equal saltiness were measured, the optimum conditions for the preparation of chinese whole cabbage kimchi were determined with the use of the response surface methodology. The results are summarized as follows; 1. The more salt concentration in brine, the longer brining time and storage period, resulted in the lower pH and the higher titratable acidity of kimchis. As the salt concentration in brine and brining time increased, kimchi reaches at optimum titratable acidity of it within a shorter time. 2. As the salt concentration in brine and brining time increased, content of succinic acid decreased but that of lactic, acetic, and propionic acid increased. Amount of citric, malic and succinic acid decreased but that of lactic, acetic acid increased, with storage day. 3. Sensory data showed that firmness and green cabbage flavor of kimchi decreased while toughness, carbonic mouthfeel, sourness and staled flavor increased with increased salt concentration in brine, brining time and storage day. As the storage period increased, crispness of kimchi decreased. 4. The optimum conditions for the preparation of chinese whole cabbage kimchi were as follows: Optimum salt concentration in brine, brining time, and storage period were 19.5%, 3 hours and 45 minutes, and 12 hours.

• 한국식품과학회지
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• 제29권6호
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• pp.1275-1280
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• 1997

차엽카테킨을 김치 천연보존제로서의 이용가능성을 검토하기 위하여 차엽에서 분말형태로 차엽카테킨을 조제하였다. 이 차엽카테킨을 김치산패에 관여하는 미생물인 St. feacalis, Leuc. mesenteroides, L. plantarum, L. brevis, P. cerevisiae와 Sachh. cerevisiae를 대상으로 항미생물 활성을 검정한 결과, Leuc. mesenteroides, L. plantarum, P. cerevisiae에 대해서는 2 mg/mL 첨가수준, St. faecalis는 4 mg/mL 첨가수준, L. brevis는 5 mg/mL 첨가수준에서부터 균의 생육이 억제되기 시작하였으나 Sacch. cerevisiae에 대한 생육억제효과는 낮았다. 차엽카테킨을 배추김치와 물김치에 첨가시기와 첨가수준을 달리하여 첨가하여 숙성중의 pH 및 산도의 변화를 측정한 결과, 두종의 김치 모두 2 mg/g 첨가수준 이상에서 pH저하와 산도증가가 억제되어, 김치의 천연보존제 및 산패억제제로서 차엽카테킨의 이용가능성이 시사되었다.

• 한국포장학회지
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• 제30권1호
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• pp.23-30
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• 2024

곤드레의 이용과 김치의 다양성을 모색하기 위해 강원도의 특산물인 곤드레를 김치제조에 이용하고자 하였다. 곤드레는 당과 단백질이 풍부한 식품으로 김치제조에 생 또는 삶은 형태로 이용을 검토한 결과 일반 성분은 비슷하였지만 항산화성이 생 곤드레가 삶은 곤드레보다 1.2배 많은 것으로 나타나 김치제조에 생 곤드레를 첨가하기로 하였다. 생곤드레를 배추김치에 20, 40 및 50 (w/w) 첨가하여 저장실험한 결과 pH, 산도 및 미생물의 변화는 곤드레의 첨가량과 관계없이 유사한 경향을 보였고 관능검사를 한 결과 곤드레를 20% (w/w) 첨가한 김치가 맛, 향, 색 및 전체적인 기호도에서 우수한 것으로 나타나 향후 김치 제조에 곤드레의 이용이 가능할 것으로 사료되었다.

• 동아시아식생활학회지
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• 제15권1호
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• pp.11-19
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• 2005

This study was conducted to provide data on awareness and use of traditional foods, suggesting the desirable ways to succeed and develop traditional foods, and the possible direction of policies and applications for food industry. The study also aimed to figure out awareness of traditional food and dietary life behaviors of married women in order to offer or draw attention to new information for better healthy life styles. Five hundred and ten married women in parts of Seoul and Kyongki area were surveyed to figure out their awareness and use of traditional foods including some of fermented foods, such as Kimchi, Doenjang(soybean paste), and Gochujang(hot pepper paste). The results were summarized as follows. First the overall satisfaction degree with traditional foods reached as high as 74.4%. They preferred traditional foods because they are healthy(38.8%), seasonally celebrated(29.9%), and well known for a variety of fermented foods(28%). Second, Kimchi, Doenjang and Gochujang, home-made or factory manufactured in an old-fashioned way, were preferred. Third, the most frequently cooked traditional foods were Kimchi Chigae and Doenjang Chigae. The most preferred cooking methods using Gochujang were Jorim(stewing) and Bokkeum(roasting). These results showed that Kimchi, Doenjang, and Gochujang were still used in cooking by married women in parts of Seoul and kyongki area, which says those are still the traditional foods most of women think as important and essential. The producers of traditional foods have to consider the safety and improvement of taste and quality of ingredients in addition to the convenience in cooking, More researches should be conducted to enhance these aspects and to develop functional foods to satisfy various needs of consumers and to improve their health.

• 한국축산식품학회지
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• 제35권3호
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• pp.339-349
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• 2015

The objective of this study was to identify lactic acid bacteria (LAB) isolated from kimchi and to evaluate its characteristics and functional properties for application in fermented dairy products as a probiotic or commercial starter culture. Eight stains isolated from kimchi were selected through an investigation of phenotypic characteristics. Two strains (DK211 and DK303) were identified as Lactobacillus plantarum, another two (DK207 and DK215) as Lactobacillus paracasei, and one (DK301) as Lactobacillus sakei. The remaining three strains were identified as species of Weissella. All selected Lactobacillus strains had acid and bile tolerance, even though there was wide variation in the ability of each strain. DK303 showed a remarkably higher proteolytic activity. There were no significant differences in β-galactosidase activity among the tested strains, except that DK301 showed no activity. Auto-aggregation varied between 82.1 and 90.0%, and hydrophobicity values ranged from 0.5 to 51.6%.The strongest auto-aggregation and hydrophobicity were observed in DK211. All selected strains showed better 1,1-diphenyl-2-picrylhydrzyl (DPPH) scavenging activity than commercial strains. DK211, DK215, DK301, and DK303 had effective inhibitory activity against all pathogens tested except E. coli. When selected strains were used for yogurt preparation as a single starter culture, the time required to reach target titratable acidity (0.9) was 11-12 h. The yogurt fermented with DK211 had favorable panelists ratings for most sensory attributes, which were comparable with yogurt fermented with a commercial strain. The results suggest that strains isolated from kimchi could be potential probiotic and starter cultures for use in yogurt manufacturing.

• 한국식품영양과학회지
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• 제29권5호
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• pp.814-821
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• 2000

나박김치의 저장성 향상을 위한 천연보존제로서 이용 가능성을 검토하기 위해 오미자 물추출물을 제조하여 이들 시료의 농도를 0.5, 1.0, 1.5, 2.0%로 사용하여 항산화력 및 전자공여능을 측정하고 나박김치 발효에 관여하는 젖산균에 대해 항균활성을 조사하였다. 항산호력은 TBA값을 측정하였는데, 오미자 물추출물의 농도가 높을수록 항산화 효과가 뚜렷이 나타났고, 시간이 경과할수록 그 효과는 현저하였다. 전자공여능 또한 오미자 물추출물의 농도가 높을수록 높은 전자공여능을 나타냈고, 1.0% 이상이 높은 전자공여능을 보였다. 나박김치에서 잦산균을 분리한 결과 117균주를 1차 분리하였고, 이 가운데 성장이 우수하고 산 생성력이 강한 4균주를 2차 선별하여 Lactobacillus brevis, Lactococcus faecalis, Leuconostoc mesenteroides, Lactobacillus plantarum을 확인하였다. 이들의 항균활성을 측정한 결과 Lactobacillus brevis를 제외한 세 균주 모두 오미자 물추출물 첨가에 의한 뚜렷한 성장 억제현상을 나타내었다. 따라서 나막김치 담금시에 항산화성과 항균활성이 있는 오미자 물추출물을 이용할 수 있는 가능성이 높을 것으로 생각되었다.

• 한국산업융합학회 논문집
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• 제2권1호
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• pp.91-95
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• 1999

The Streptomyces albulus broth, when the polylysine in the broth, that has powerful growth inhibiting effect far many microbes, is its maximum, had filtered off the cells, to use the broth as preservative for keeping favorable taste of Korean Kimchi. Some microorganisms in their specific growth medium, known to deteriorate the useful nutrient of the Kimchi, has grown with different amounts of the inhibiting broth, to determine the minimum growth inhibition concentration. The ${\varepsilon}$-poly-L-lysine had been identified from the IR spectroscopic analysis of the purified poly lysine of the broth from ion exchange chromatographic separation. The content of the polylysine had been determined by methyl orange decoloration effect. Though the minimum inhibition concentration, evaluated by the naked eye based on the conventional method measuring the turbid feature after 18 hours of culture, has different values each other, the observed effects confirmed that the crude broth could be used as a natural preservative for the Kimchi in extending the fair taste.

• KSBB Journal
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• 제26권5호
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• pp.374-380
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• 2011

Earlier in Korea Kimchi was made in every family and every province has own taste and specialties. These days almost of the Kimchis are manufactured. We collected variable Kimchis, which were made for private use and isolated microorganisms. Some interesting micobial cells were identified and studied for its application as food and drinks. One of them was identified as Lactobacillus sakei KJ123. This strain is known as producing interesting aromatic components during Sakei fermentation like Kimchi in variable conditions. We tried to develop a health beverage with fermentation process. The Cucurbita maxima has been known as a traditional healthy food and variable positive effects on the human body were already reported. In this study we tried to develop a production process for a healthy fermented drink on this substrate with strains originated from Kimchi. Many kinds of lacctobacilli species existed in the fermented food cannot survive in the acidic conditions like human stomach. So we selected resisting strains in this conditions. The survival rate of Lactobacillus sakei cells in the artificial gastric juice and bile acid and other physiological characteristics at the variable conditions have been tested. After fermentation process some sensory tests on the product with panels were tried.

• 한국식생활문화학회지
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• 제10권4호
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• pp.311-319
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• 1995

The history of vegetable preservation technology by salting and fermentation in Korea was reviewed from the Three Nations Era to the end of Chosun Kingdom, and the development of present day's Kimchi processing technology was traced back by using the classic books as well as recent review papers published in Korea. Although the written record on salting and fermentation of vegetables first appears in a 12th century literature (Dongkukisangkukjib, Gyu-Bo Lee $1168{\sim}1241$), the use of salted/fermented vegetables could be dated back to the Three Nations Era $(B.C.\;37{\sim}A.D.\;668)$ and even earlier period. The present type of Kimchi was gradually evolved after the introduction of red pepper into Korea in the 17th century. The descriptions on Kimchi fermention appeared in the literatures written in the period of $16th{\sim}19th$ centuries in Korea, Suunjapbang $(1500{\sim})$, Domundaijak (1611), Sasichanyocho (1656), Eumsikdimibang (1670), Chubangmun $(1600{\sim})$, Saekgyung (1676), Yorok $(1600{\sim})$, Sanlimkyungje (1715), Cheungbosanlimkyungje (1766), Kyuhapchongsoe $(1800{\sim})$, Imwonsipyukji (1827), Dongkuksesiki (1849) and Buinpylji $(1855{\sim})$ were reviewed.