• 대한생식의학회:학술대회논문집
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• 2002.11a
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• pp.105-106
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• 2002

• 대한생식의학회:학술대회논문집
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• 2002.11a
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• pp.103-103
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• 2002

• 대한생식의학회:학술대회논문집
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• 2002.05a
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• pp.74.2-75
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• 2002

• 대한생식의학회:학술대회논문집
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• 2002.05a
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• pp.90-90
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• 2002

• 대한생식의학회:학술대회논문집
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• 2002.05a
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• pp.91.2-92
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• 2002

• Proceedings of the Korean Nuclear Society Conference
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• 2005.10a
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• pp.71-72
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• 2005

• Proceedings of the Korean Vacuum Society Conference
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• 2006.08a
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• pp.68-68
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• 2006

• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.4
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• pp.965-972
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• 2005

To investigate the effects of Trichosanthes semen extract on the growth and apoptosis of human uterine cervical carcinoma cells. Effects of Trichosanthes semen extract on the growth of ME-180 cells were assayed by MTT assay. Apoptosis induced by Trichosanthes semen extract was detected by fluorescent microscopy, DNA fragmentation analysis and flow cytometry. Caspase-3 and caspase-8 activities were assayed. Trichosanthes semen extract induced ME-180 cells to die in a dose- and time-dependent manner. ME-180 cells treated with Trichosanthes semen extract exhibited typical characteristics of apoptosis. The population of Sub-G1 cells increased significantly, and the cells represented the reduced size, condensed chromatin and apoptotic bodies. They showed the decreased mitochondrial membrane potential and increased activities of caspase-3 and caspase-8. The results suggest that Trichosanthes semen extract induced ME-180 cell apoptosis and the activation of caspase and mitochondrial pathway were involved in the process of Trichosanthes semen extract-induced apoptosis.

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.1
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• pp.1-13
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• 2006

Purpose : Polygonum cuspidatum extract is an oriental herb which has been used for uterine diseases. In this study, the effects of Polygonum cuspidatum extract were investigated on inducing growth inhibition and apoptosis of human uterine cervical carcinoma cells. Methods : Viability of Polygonum cuspidatum extract-induced ME-180 cells was measured by MTT assay. Apoptotic cells were visualized by EtBr/AcOr staining under fluorescent microscope. Nucleosomal DNA fragmentation was assayed by agarose gel electrophoresis. Cell cycle distribution and changes in mitochondrial membrane potential were observed by flow cytometry. Results : Polygonum cuspidatum extract induced ME-180 cell death in a dose- and time-dependent manner. In the cells treated with Pc, the population of cells at sub-G1 phase significantly increased, and the condensed nuclei, apoptotic bodies and nucleosome-sized DNA were detected. Moreover, reduction in mitochondrial membrane potential was detected. Conclusion : Polygonum cuspidatum extract inhibits the growth and proliferation of ME-180 cells by apoptotic induction and facilitates its activity initiated by depolarization of mitochondria.

• Korean Journal of Environmental Agriculture
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• v.38 no.2
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• pp.96-103
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• 2019

BACKGROUND: This study was conducted to develop selenium (Se)- and germanium (Ge)-enriched rice by foliar spray application of organic or inorganic Se and Ge. METHODS AND RESULTS: The time and frequency of organic or inorganic Se and Ge treatment were performed at the five main growth stages as followings: effective tillering stage (E), maximum tillering stage (M), booting stage (B), heading stage (H), grain filling stage (G). The main treatment plots were consisted of (1) 'once' treatment (at each E, M, B, H, G stage, Se/Ge single apply), (2) 'twice I' (at H + G stages, organic or inorganic Se/Ge apply), (3) 'twice II' (at H + G stages, mixture apply of Se + Ge + pesticide). The organic or inorganic Se treatment concentration was 20 and 40 ppm, and the Ge was 50 and 100 ppm. The Se and Ge contents in rice grain (brown rice and polished rice) were analyzed by inductively coupled plasma (ICP). The highest Se content was noted in brown rice 'twice I' with Se 40 ppm (1394.06) at H + G stages, but the lowest was in 'once' with Se 40 ppm ($367.79{\mu}g{\cdot}kg^{-1}$) at B stage. The highest of Se content in polished rice was found in 'twice I' of Se 40 ppm (1090.25) at H + G stages, but the lowest was in 'once' with Se 40 ppm ($403.53{\mu}g{\cdot}kg^{-1}$) at E stage. On the other hand, The highest of Ge content in brown rice was found in 'twice I' with Ge 100 ppm (398.66) at H + G stages, but the lowest was in 'once' with Ge 100 ppm ($139.64{\mu}g{\cdot}kg^{-1}$) at B stage. The highest of Ge content in polished rice was found in 'twice I' of Ge 100 ppm (300.29) at H + G stages, but the lowest was in 'once' with Ge 100 ppm ($142.24{\mu}g{\cdot}kg^{-1}$) at B stage. CONCLUSION: Se and Ge contents both in brown rice and polished rice treated with organic Se and Ge forms were higher than those of inorganic Se and Ge. Overall results concluded that the supplementation of organic Se and Ge contents in brown and polished rice contents were comparatively higher than the inorganic Se and Ge. This is results also proved that the foliar spray application of organic Se and Ge has positive nutritive effect on the rice for regular consumption.