• Title/Summary/Keyword: Kim Jeong-hee

Search Result 10,204, Processing Time 0.059 seconds

Mechanism of Anti-Invasive Action of Docosahexaenoic Acid in SW480 Human Colon Cancer Cell (인체 대장암 세포주 SW480에서 docosahexaenoic acid에 의한 침윤억제 기전)

  • Shin, So-Yeon;Kim, Yong-Jo;Song, Kyoung-Sub;Jing, Kaipeng;Kim, Na-Yeong;Jeong, So-Yeon;Park, Ji-Hoon;Seo, Kang-Sik;Heo, Jun-Young;Kwon, Hyun-Joo;Park, Jong-Il;Park, Seung-Kiel;Kweon, Gi-Ryang;Yoon, Wan-Hee;Hwang, Byung-Doo;Lim, Kyu
    • Journal of Life Science
    • /
    • v.20 no.4
    • /
    • pp.561-571
    • /
    • 2010
  • Colon cancer is one of the most common malignancies in the western world and the second leading cause of cancer death in Korea. Epidemiology studies have shown a reduced incidence of colon cancer among populations consuming a large quantity of ${\omega}3$-polyunsaturated fatty acids (${\omega}3$-PUFA) of marine origin. Recently, it has been found that ${\omega}3$-PUFA has an antineoplastic effect in several cancers. This study was designed to investigate the mechanism of the anti-invasive effect of ${\omega}3$-PUFA in colon cancer. ${\omega}3$-PUFA, docosahexaenoic acids (DHA) and eicosapentaenoic acid (EPA) treatment resulted in a dose-dependent inhibition of cell growth in SW480 human colon cancer cells. In contrast, arachidonic acid (AA), a ${\omega}6$-PUFA, exhibited no significant effect. This action likely involves apoptosis, given that DHA treatment increased apoptotic cells in TUNEL assay. Moreover, invasiveness of SW480 cells was inhibited following treatment of DHA in a dose-dependent manner; in contrast, AA had no effect. The levels of MMP-9 and MMP-2 mRNA decreased after DHA pretreatment. MMP-9 and MMP-2 promoter activities were also inhibited by DHA treatment. The levels of NF-kB and p-IkB protein were down-regulated by DHA pretreatment in a dose dependent manner. In addition, DHA inhibited NF-kB promoter reporter activities. These findings suggest that ${\omega}3$-PUFA may inhibit cancer cell invasion by inhibition of MMPs via reduction of NF-kB in colon cancer. In conclusion, ${\omega}3$-PUFA could be used for chemoprevention and treatment of human colon cancer.

Effects of Nitrogen Fertilization Increment on Forage Crops Cultivation in Saemangum Reclaimed Land (새만금간척지 사료작물 재배시 질소증비 효과)

  • Yang, Chang-Hyu;Kim, Sun;Lee, Jang-Hee;Baek, Nam-Hyun;Kim, Taek-Kyum;Choi, Weon-Young;Jeong, Jae-Hyuk;Lee, Sang-Bok;Lee, Gyeong-Bo
    • Korean Journal of Soil Science and Fertilizer
    • /
    • v.45 no.2
    • /
    • pp.235-240
    • /
    • 2012
  • This study was conducted to find out the optimum cropping system for the stable production of forage crops in the newly reclaimed land located at Gwanghwal region of Saemangum reclaimed land in which the soil is sandy loam (Munpo series). There were two treatments of nitrogen fertilization 20% increment based on the standard fertilization of 150, $200kg\;ha^{-1}$. Whole crop barley as the winter crop sowed on 27 October. After the whole crop barley was harvested at the end of May. Corn and sorghum${\times}$sudangrass as the summer crop sowed at the early of June successively on the same field. Emergence rate the whole crop barley was high while the summer crops were low. Soil salinity was increased during cultivation of summer crops. However, corn and sorghum${\times}$sudangrass were not damaged by salt. Increase of nitrogen fertilization made the growth of cultivation crops good, stem and leaf tended to have a lot of the mineral nutrients at heading stage and silking stage. After experiment, among soil chemical properties pH, content of exchangeable sodium were decreased and content of organic matter, available phosphate were increased. Dry matter yield were showed whole crop barley $13,170kg\;ha^{-1}$ and sorghum${\times}$sudangrass $19,440kg\;ha^{-1}$ by increment of nitrogen fertilization. Therefore, to improve the product and nutrient balance of reclaimed saline land comprehensive soil management should be considered.

Immuno-stimulatory Activities of a High Molecular Weight Fraction from Cynanchum wilfordii Radix Obtained by Ultrafiltration (한외거르기(Ultrafiltration)에 의하여 분리된 백수오 고분자 분획물의 면역증진 활성)

  • Jang, Mi;Lim, Tae-Gyu;Hong, Hee-Do;Rhee, Young Kyoung;Kim, Kyung-Tack;Lee, Eunjung;Lee, Jeong Hoon;Lee, Yun Ji;Kim, Yeon Bok;Cho, Chang-Won
    • Korean Journal of Food Science and Technology
    • /
    • v.48 no.3
    • /
    • pp.268-274
    • /
    • 2016
  • The purpose of this study was to investigate the immuno-stimulatory activity of the high-molecular-weight fraction (HMWF) of Cynanchum wilfordii (CW) extracts obtained by ultrafiltration in murine macrophage RAW 264.7 cells and to assess its immuno-stimulatory effect in mice. Ultrafiltration was performed with polyethersulfone membranes (30 kDa cutoff) in a cross-flow filtration system to obtain the HMWF of CW. The results showed that the HMWF increased the production of various cytokines such as tumor necrosis factor-${\alpha}$, interleukin-6, and nitric oxide in dose-ependent manners. In addition, HMWF treatment increased the relative spleen weight as well as splenocyte proliferation induced by concanavalin A or bacterial lipopolysaccharide in mice. Natural killer (NK) cell activity in the HMWF-treated group was significantly increased compared to that in the control group. These results suggest that the HMWF of CW can support the immune system through secretion of macrophage cytokines, thereby enhancing NK cell activity and murine splenocyte proliferation.

A Study on the removal of nitrogen by combined nitrification and autotrophic denitrification (질산화와 무기영양 독립탈질화의 연계처리에 의한 질소제거에 관한 연구)

  • Han, Gee-Bong;Jeong, Da-Young;Woo, Mi-Hee;Kim, So-Yeon;Kim, Bio
    • Journal of the Korea Organic Resources Recycling Association
    • /
    • v.16 no.2
    • /
    • pp.74-80
    • /
    • 2008
  • Removal of nitrogen compound under nitrification related with denitrification by biofilm which developed on the porous media was investigated. With the investigation of $NH_4-N$ nitrification and autotrophic denitrification supplied with sulfur media as electron donor, conclusions were retrieved as follows. When $F/M_N$ ratio of $NH_4-N$ was increased from $0.0062-0.034gNH_4-N/g\;MLVSS{\cdot}day$ by the change of influent concentration and HRT the nitrification rate decreased as the increase of loading rate. Also under the same conditions of $F/M_N$ ratio, the alkalinity consumption rate of operation was higher at 8 hours of HRT than at 6 hours of HRT. Accordingly the influent loading rate variation by detention time with influent flow influenced more on the nitrification efficiency than the influent loading rate variation by the influent concentration did. Denitrification rate with various EBCT(Empty Bed Contact Time) showed average 25% at 8.4hrs of EBCT but sharply decreased average 5% at 4.6hrs of EBCT, so the operation would be more effective at above 8.4hrs of EBCT. Also denitrification rate was known to be adversely increased as $NO_3-N$ loading rate per unit volume of sulfur-media was decreased within the range of $0.5{\sim}2.0kgNO_3-N/m^3{\cdot}day$.

  • PDF

Distribution of Micronutrients in Plastic Film House Soils of Yeongnam Provincen (영남지역 시설재배지 토양의 미량원소 함량 분포)

  • Chung, Jong-Bae;Kim, Bok-Jin;Ryu, Kwan-Sig;Lee, Seung-Ho;Shin, Hyun-Jin;Hwang, Tae-Kyung;Choi, Hee-Youl;Lee, Yong-Woo;Lee, Yoon-Jeong;Kim, Jong-Jib
    • Korean Journal of Soil Science and Fertilizer
    • /
    • v.39 no.4
    • /
    • pp.214-223
    • /
    • 2006
  • For better management of micronutrients in crop cultivation, the availability of micronutrients in the soils must be updated and evaluated as a first step. This study was conducted to investigate the distribution of micronutrients in soils of plastic film houses. Total 396 soil samples were collected from the plastic film houses of various crops in Yeongnam province (strawberry, 96; red pepper, 66; tomato, 74; oriental melon, 97; cucumber, 63). Total and available contents of B, Cu, Zn, Fe, and Mn in the soils were determined. Available B was extracted with hot water and available Cu, Zn, Fe, and Mn were extracted with 0.1 N HCl. Mean values of total contents of B, Cu, Zn, Fe, and Mn in the plastic film house soils were 25, 32, 74, 21,316, and $420mg\;kg^{-1}$, respectively. Total contents of micronutrients in the plastic film house soils were similar to those found in the open fields nearby, while they were different among the locations investigated. Mean contents extractable B, Cu, and Zn in the plastic film house soils were 2.1, 7.5, and $35mg\;kg^{-1}$, respectively. The contents of extractable B, Cu, and Zn in the plastic film house soils were higher than those found in the open fields nearby with exceptions of B in soils of strawberry and Cu in soils of red pepper and oriental melon. However, mean contents of extractable Fe and Mn in the plastic film house soils were 156 and $146mg\;kg^{-1}$, respectively, and the mean content of extractable Fe was much lower than that found in open fields nearby. The contents of extractable Zn, Fe, and Mn were higher than the sufficient levels for the crop requirements in most of the plastic film house soils investigated. Contents of extractable Cu in most soils of strawberry, tomato and cucumber cultivations were higher than the sufficient level. However, extractable Cu contents were below the sufficient level in about 30% of investigated soils of red pepper and oriental melon cultivation. Soils containing higher contents of extractable B than the sufficient level were relatively fewer in comparison to the other micronutrients.

Control effect of the Mixture of Bacillus amyloliquefaciens M27 and Plant Extract against Cucumber Powdery Mildew (Bacillus amyloliquefaciens M27과 식물천연물 혼합제에 의한 오이 흰가루병의 방제 효과)

  • Lee, Sang Yeob;Weon, Hang Yeon;Kim, Jeong Jun;Han, Ji Hee;Kim, Wan Gyu
    • The Korean Journal of Pesticide Science
    • /
    • v.17 no.4
    • /
    • pp.435-439
    • /
    • 2013
  • Bacillus amyloliquefaciens M27 was selected as a control agent for the biological control of cucumber powdery mildew. The new mixture of B.amyloliquefaciens M27 and plant (Eucalytus) extract was developed to improve the control activity of B.amyloliquefaciens M27 against cucumber powdery mildew. The mixed formulation showed the high preventive and curative control effect against cucumber powdery mildew when it was diluted at 500 times and foliar-sprayed. Its control effect was higher in preventive spraying than curative spraying. When 500-fold diluted solution of the formulation was sprayed preventively four times at five-day intervals, three times at seven-day intervals and twice at ten-day intervals, the diseased leaf area was shown to be 4.4%, 8.0%, 27.9%, respectively; Whereas the diseased leaf area in the control plot was 45.4%. When the 500-fold diluted formulation was sprayed curatively four times at five-day intervals, three times at seven-day intervals and twice at ten-day intervals after occurred cucumber powdery mildew, the diseases leaf area was 11.5%, 25.2%, 51.8%, respectively; whereas in the control plot, the diseases leaf area was 64.3%. When the 500-fold diluted formulation was treated four times at five-day intervals in the plastic house, its control effect was higher than that treated three times at seven-day intervals and twice at ten-day intervals. As the results, the mixed formulation of B.amyloliquefaciens M27 and plant extract could be a promising candidate of bio-fungicides for the environment-friendly control of powdery mildew of cucumber.

Evaluation of Crop Characteristics of Sorghum (Sorghum bicolor L.) Germplasm for the Selection of Excellent Resources (우수자원 선발을 위한 수수(Sorghum bicolor L.) 유전자원의 특성평가)

  • Yoon, Seong-Tak;Jeong, In-Ho;Han, Tae-Kyu;Kim, Young-Jung;Yu, Je-Bin;Yang, Gyeong;Ye, Min-Hee;Baek, Seung-Woo;Kim, Kun-Woo
    • Korean Journal of Plant Resources
    • /
    • v.29 no.4
    • /
    • pp.479-494
    • /
    • 2016
  • The aim of this study is to select the superior resources of high yield, high content of functional material optimal to mechanical harvesting by the evaluation of crop growth and yield characteristics in sorghum germplasm. One hundred accessions of sorghum germplasm were used in this experiment. Days from seeding to heading date showed the range from 68 to 94 days with the highest frequency proportion was the group from 80 to 85 days, which occupied 34% (34 plant resources) of 100 germplasm. Ear types of 100 sorghum germplasm could be classified as 7 types of broom-tillering, half broom-tillering, extreme open-loose type, open-loose type, intermediate type, compact type, extreme-compact type of which intermediate type was the highest ratio of 28% (28 plant resources) of 100 germplasm. Yield showed the range from 106 to 365 ㎏/10a with the highest frequency proportion of it was the group from 150 to 200 ㎏/10a, which occupied 44% (44 plant resources) of 100 germplasm. Among 100 sorghum germplasm, 18 ideal resources of high yield and short plant height appropriate for mechanical harvesting were selected. In order to evaluate high content of functional substance, selected 18 resources were analyzed for total polyphenol content, DPPH radical scavenging activity and total anthocyanin content. Finally, we selected 5 resources of short plant height, high yield, high content of total polyphenol and high DPPH radical scavenging activity among 18 genetic resources.

Effect of Nitrogen Fertigation on The Growth and Nutrition Uptake of 'Brightwell' Rabbiteye Blueberry (질소관비 수준이 래빗아이 블루베리 '브라이트웰'의 생장과 양분흡수에 미치는 영향)

  • Kwack, Yong-Bum;Chae, Won-Byoung;Lee, Mock-hee;Jeong, Hae-Won;Rhee, Han-Cheol;Kim, Jin-Gook;Kim, Hong-Lim
    • Korean Journal of Environmental Agriculture
    • /
    • v.36 no.3
    • /
    • pp.161-168
    • /
    • 2017
  • BACKGROUND: Rabbiteye blueberry(Vaccinium ashei Reade) has low nutrient uptake efficiency due to its shallow and fibrous root system without root hairs. This study was carried out to establish an efficient nutrient application standard by investigating the effect of nitrogen fertigation on the growth and fruit characteristics of rabbiteye blueberry. METHODS AND RESULTS: 'Brightwell' rabbiteye blueberry was treated with 0, 50, 100 and 200% nitrogen fertigation of recommended fertilizer application (6, 9 and 14 g/bush in the first, second and third years, respectively). The results showed that leaf nitrogen content significantly correlated with the fruit weight and fruit yield. However, canopy area, dry weight, sugar and anthocyanin contents did not correlate significantly with the leaf nitrogen content. The leaf and stem dry weights of 'Brightwell' rabbiteye blueberry during the third year of planting were the highest with 50% nitrogen fertigation (leaf dry weight=723.7 g/bush; stem dry weight=890.7 g/bush). Maximum fruit yield of 'Brightwell' rabbiteye blueberry (12.9 kg/bush) was observed during the third year of planting with 50% nitrogen fertigation and this was about 70% greater than the treatment that received no nitrogen fertigation. The fruit yields of 'Brightwell' rabbiteye blueberry during the third year of planting treated with 100 and 200% nitrogen fertigation were 11.0 and 11.5 kg/bush, and these were 17 and 12%lower than the 50% nitrogen fertigation treatment, respectively. Further, the efficiency of nitrogen utilization was the highest (90%) with 50% nitrogen fertigation and lowest (18%) with 200% nitrogen fertigation. CONCLUSION: The results of this study suggests that fertigation with 50% of the recommended fertilizer could be most effective for enhancing the growth and nitrogen use efficiency of rabbiteye blueberry.

Effect of Calcium Ion and Initial pH on Phosphorus Removal using TiCl4 Coagulant (칼슘이온과 초기 pH가 TiCl4 응집제의 인 제거에 미치는 영향)

  • Kang, Seong Hyeon;Kang, Jang Hyeon;Kim, Han Gyul;Jeong, Hee Hoon;Kim, Jong Ho;Ahn, Johng-Hwa
    • Journal of Korean Society of Environmental Engineers
    • /
    • v.39 no.3
    • /
    • pp.164-168
    • /
    • 2017
  • This study experimentally determined the effect of calcium ion ($Ca^{2+}$) (0-0.55 mM) and intial pH (4.0-10.0) on phosphorus (P) removal in synthetic wastewater (2 mg P/L) using titanium tetrachloride ($TiCl_4$) (0.12-0.18 mM). At TiCl4 concentration ([$TiCl_4$]) = 0.12 mM, the P removal efficiency was the highest (95.1%) at pH 7 but the efficiency decreased to 51.4% at pH 8. The P removal efficiency was 55.6% at $Ca^{2+}$ concentration ([$Ca^{2+}$]) = 0 mM but the efficiency increased to 90.5% at [$Ca^{2+}$] = 0.045 mM at [$TiCl_4$] = 0.12 mM. On the other hand, the P removal efficiency difference was not large (96.5-99.5%) with [$Ca^{2+}$] at [$TiCl_4$] = 0.15-0.18 mM. Within the design boundaries of $0.00{\leq}[Ca^{2+}]{\leq}0.18mM$ and $7.0{\leq}initial$ $pH{\leq}9.0$ at [$TiCl_4$] = 0.12 mM, the 90% P removal efficiency could be achieved at $[Ca2+]{\geq}0.10mM$ with pH 8.0 and $[Ca2+]{\geq}0.12mM$ with pH 9.0.

An Improved Analytical Method for the Determination of Qualitative and Quantitative Characteristics of Di- and Trisaccharides in Honey using GC and GC/MS (GC 및 GC/MS에 의한 벌꿀 중의 이·삼당류 정성 및 정량 특성과 개선된 동시분석방법의 확립)

  • Kim, Jong-Bae;Jang, Eun-Suk;Kim, In-Suk;Lee, Hee-Jin;Lee, Hye-Jeong;Seo, Hyun-Sun;Park, Nam-Pyo
    • Korean Journal of Food Science and Technology
    • /
    • v.47 no.1
    • /
    • pp.27-36
    • /
    • 2015
  • The improved analytical method with gas chromatography (GC) and GC-mass spectrometry was established to identify and quantify disaccharides and trisacchrides in honey. In this method, the analysis of trimethylsilyl (TMS), TMS-oxime and TMS-methoxime sugars takes into account the determination of a single peak of complete separation on the chromatogram. The number of possible peaks for the qualitative and quantitative determination of TMS, TMS-oxime, and TMS-methoxime sugars was 17, 22, and 25, respectively. This new analytical method allowed for the determination of diand trisaccharides in honey by TMS-oxime and TMS-methoxime derivatives. This study suggested that the improved method is more suitable and precise than the other analytical methods for the simultaneous determination of sugars in honey.