• Journal of Bio-Environment Control
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• v.14 no.4
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• pp.233-238
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• 2005

Growth of Campanula punctata 'Rubriflora' plantlets, as affected by three levels of photosynthetic photon flux (PPF), 70, 110, and $220{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, two levels of $CO_2$ concentration, 500 and $1,500{\mu}mol{\cdot}m^{-1}$, and two levels of number of air exchanges per hour (NAEH), 0.1 and $2.8 h^{-l}$, was studied. Explants were obtained from photomixotrophically-micropropagated plantlets. Four explants were planted in each $3.7{\times}10^{-4}m^3$ polycarbonate box containing MS basal medium and no added sucrose. Explants were cultured under cool-white fluorescent lamps for $16h{\cdot}d^{-1},\;at\;25\pm1^{\circ}C$ temperature, and $70\~80\%$ relative humidity In treatments of $2.8h^{-1}$ NAEH, a 10mm round hole made on the vessel cap was sealed with a microporous filter. For higher $CO_2$ concentrations in the culture room, $CO_2$ gas was provided from a tank of liquefied $CO_2$. Fresh and dry weights, length of the longest root, and number of leaves significantly increased with increasing PPF and especially $CO_2$ concentration. Length of the longest root, number of leaves, fresh and dry weights, and chlorophyll concentration were enhanced with increased NAEH. However, leaf area was the smallest in the $220{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}\;PPF\;2.8h^{-1}$ NAEH and especially, $1,500{\mu}mol{\cdot}mol^{-1}\;CO_2$ concentration treatment. Treatment effect became more produced with time. Overall, treatment with $220{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}\;PPF\;and\;1,500{\mu}mol{\cdot}mol^{-1}\;CO_2$ gave the most vigorous growth.

• Korean Journal of Environmental Agriculture
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• v.34 no.4
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• pp.274-281
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• 2015

BACKGROUND: A lasting release of low levels of persistence chemicals including pesticides and pharmaceuticals into river has a bad influence on aquatic ecosystems and humans. The present study monitored pesticide residues in the Yeongsan and Seomjin river basins and their tributaries as a fundamental study for water quality standard of pesticides.METHODS AND RESULTS: Nine pesticides(aldicarb, carbaryl, carbofuran, chlorpyrifos, 2,4-D, MCPA, methomyl, metolachlor, and molinate) were determined from water samples using SPE-Oasis HLB(pH 2) and LC/MS/MS. Validation of the method was conducted through matrix-matched internal calibration curve, method detection limit(MDL), limit of quantification(LOQ), accuracy, precision, and recovery. MDLs of all pesticides satisfied the GV/10 values. Linearity(r²) was 0.9965- 0.9999, and a percentage of accuracy, precision, and recovery was 89.4-113.6%, 3.1-14.0%, and 90.8-106.2%, respectively. All pesticides exclusive of aldicarb were determined in the river samples, and there was a connection between the positive monitoring results and agricultural use of the pesticides.CONCLUSION: Monitoring outcomes of the present study implied that pesticides were a possible non-point pollutant source in the Yeongsan and Seomjin river basins and tributaries. Therefore, it is required to produce and accumulate more monitoring results on pesticides in river waters to set water quality standards, finally to preserve aquatic ecosystems.

• Applied Microscopy
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• v.18 no.1
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• pp.1-20
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• 1988

There's been arguments on the different morphological status between the nucleus accumbens septi and nucleus fundus striati of ventral striatum. Authors carried out the comparative study on the neuronal cell types of these nuclei, in the chick and the rat. Results are summarized as follows: In the nucleus accumbens septi of the chick, there found 3 main cell types. Type I cells are oval or spindle-shaped. They are the most abundant cell types, comprising more than 80% of neurons. The pale nucleus is usually indented. The cytoplasm is also pale and contains small amount of mitochondria, rough r-ER and Golgi complexes. This cell has a few symmetric synapses on the cell membrane. Type II cells are pale large cells. They are polygonal or irregularly-shaped. They contain pale spherical nucleus, and the pale cytoplasm with relatively large amount of mitochondria, free ribosomes and well-developed Golgi complexes. Some axo-somatic synapes are found on the cell. Type III cells are oval or spherical-shaped. The nucleus is relatively pale and large, In the dense cytoplasm, well developed. r-ER formed typical Nissl's body, and there found many mitochondria, ribosomes and lysosomes. In the chick fundus striati nucleus, there also found 3 main cell types. Type I cells are small and spindle-shaped. This type is the most abundant one and constitutes more the 80% of the neurons. Morphological features other than it's shape, is generally similar with that of Type I cell in the nucleus accumbens. Type II cells are irregularly shaped large cells. Dense cytoplasm contains large amount of cell organelles. Some axo-somatic synapses are found. Type III cells are small dense cells. This oval cell contains the oval nucleus, and the plentiful cytoplasm with well developed r-ER, ribosomes and mitochondria. In the nucleus accumbens septi of the rat, there found 4 main cell types. Type I cells are small, oval or spherical cells, comprising more than 90% of all the neurons. Spherical nucleus shows typical chromatin rim along the nuclear membrane. Dense cytoplasm contains many ribosomes and mitochondria. Type II cells are large oval cells. The eccentric nucleus is deeply invaginated. Pale cytoplasm contains large amount of ribosomes, Golgi complexes, mitochondria, and dense bodies. Type III cells are pale, large, oval cells. They contain moderate amount of ribosomes and mitochondria, and some scattered stacks of r-ER. Type IV cells are small pale cells. Small oval nucleus is indented and shows chromatin rim. Only small amount of ribosomes and mitochondria can be found. In the nucleus fundus striati of the rat, there also found 4 main cell types. Type I cells are spherical or oval cells, comprising more than 90% of the neurons. The chromatin rim of the spherical nucleus is not so prominent as compared to the rim of type I cell in the nucleus accumbens septi. The cytoplasm contains moderate amount of mitochondria, ribosomes and some scattered r-ER. A few axo-somatic synapses were found. Type II cells are small round or polygonal cells. Golgi complexes are especially well-developed in this cell type. The cytoplasm also contains moderate amount of mitochondria, ribosomes, and dense bodies. Type III cells are small cells. The large nucleus shows prominent chromatin rim. The cytoplasm contains many ribosomes and mitochondria. Type IV cells are large, spheircal or oval cells. The nucleus is deeply indented. The plentiful cytoplasm contains large amount of ribosomes, mitochondria, Golgi complexes, neurotubules, but not r-ER. In the present study, it is clear that the nucleus accumbens septi and the nucleus fundus striati are independant cell groups, according to their cytoarchitectonics and the ultrastructural features of their cell types.

• Science of Emotion and Sensibility
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• v.22 no.4
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• pp.97-106
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• 2019

The purpose of this study was to investigate the effect of the type and measurement location of a fabric strain gauge sensor on the detection performance for respiratory signals. We implemented two types of sensors to measure the respiratory signal and attached them to a band to detect the respiratory signal. Eight healthy males in their 20s were the subject of this study. They were asked to wear two respiratory bands in turns. While the subjects were measured for 30 seconds standing comfortably, the respiratory was given at 15 breaths per minute were synchronized, and then a 10-second break; subsequently, the entire measurement was repeated. Measurement locations were at the chest and abdomen. In addition, to verify the performance of respiratory measurement in the movement state, the subjects were asked to walk in place at a speed of 80 strides per minute(SPM), and the respiratory was measured using the same method mentioned earlier. Meanwhile, to acquire a reference signal, the SS5LB of BIOPAC Systems, Inc., was worn by the subjects simultaneously with the experimental sensor. The Kruskal-Wallis test and Bonferroni post hoc tests were performed using SPSS 24.0 to verify the difference in measurement performances among the group of eight combinations of sensor types, measurement locations, and movement states. In addition, the Wilcoxon test was conducted to examine whether there are differences according to sensor type, measurement location, and movement state. The results showed that the respiratory signal detection performance was the best when the respiratory was measured in the chest using the CNT-coated fabric sensor regardless of the movement state. Based on the results of this study, we will develop a chest belt-type wearable platform that can monitor the various vital signal in real time without disturbing the movements in an outdoor environment or in daily activities.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.29 no.4
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• pp.247-259
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• 1993

The fishing experiment was carried out by the training ship Saebada in order to analyse the mesh selectivity for trawl cod-end, in the Southern Korea Sea and the East China Sea from June. 1991 through August, 1992. The trawl cod-end used in this experiment has the trouser type of cod-end with cover net. and the mesh selectivity was examined for the five kinds of the opening of mesh in its cod-end part. A total of 163 hauls, of which having mesh size 51.2mm ; A 89, 70.2mm ; B 54, 77.6mm ; C 55, 88.0mm ; D 52 and 111.3mm ; E 20 were used respectively. Selection curves and selection parameters were calculated by using a logistic function, S=1/(1+exp super(-(aL+b)) ). The mesh election master curves were estimated by S=1/(1+exp super(-[a(L/M)+$\beta$]) ). and the optimum mesh size were calculated with (L/M) sub(50) of master curve. In these cases 'a' and '$\alpha$' are slope, 'b' and '$\beta$' are intercept. 'L' is body length of the target species of fishes, 'M' is the mesh size, and 'S' denotes mesh selectivity. In this report, the four species of compressed form fishes were taken analized according to fish shape, and 'S' denotes mesh selectivity. In this report, the four species of compressed form fishes were taken analized according to fish shape, and the results obtained are summarized as follows: 1. Red seabream Pagrus major(Temminct et Schlegel) and yellow porgy Dentex tumifrons(Temminct et Schlegel) ; Selection rate in each mesh size of A, B, C, D and E were 99.7%, 97.5%, 91.4%, 76.7% and 57.8% respectively. Selection parameters 'a' and 'b' of mesh sizes C, D and E were 2.65 and -28.62, 4.40 and -77.73, 2.31 and -46.99, and their selection factors were 1.39, 2.10, 1.83 respectively. Selection parameters of master curve '$\alpha$' and '$\beta$' were 3.05 and -5.65 respectively, and (L/M) sub(50) was 1.85. The optimum mesh size of Red seabream was 141mm. 2. Filefish Thamnaconus modestus (Gunther) ; Selection rate in each mesh size of A, B, C, D and E were 99.6%, 98.3%, 91.2%, 80.0% and 48.6% respectively. Selection parameters 'a' and 'b' of mesh sizes C, D and E were 5.82 and -55.10, 2.92 and -36.90, 3.91 and -63.09, and their selection factors were 1.35, 1.44, 1.45 respectively. Selection parameters of master curve '$\alpha$' and '$\beta$' were 3.02 and -4.32 respectively, and (L/M) sub(50) was 1.43. The optimum mesh size was 129mm. 3. Target dory Zeus faber Valenciennes ; Selection rate in each mesh size of A, B, C, D and E were 99.7%, 100%, 83.2%, 91.6% and 65.0% respectively. Selection parameters 'a' and 'b' of mesh sizes C, D and E were 3.85 and -32.46, 4.19 and -57.38, 2.45 and -40.03, and their selection factors were 1.09, 1.56, 1.47 respectively. Selection parameters of master curve '$\alpha$' and '$\beta$' were 2.64 and -3.53 respectively, and (L/M) sub(50) was 1.34. The optimum mesh size was 127mm. 4. Butterfish Psenopsis anomala (Temminct et Schlegel) ; Selection rate in each mesh size of A, B, C, D and E were 99.2%, 34.1%, 46.5%, 14.3% and 2.4% respectively. Selection parameters 'a' and 'b' of mesh sizes B, C and D were 5.35 and -71.70, 5.07 and -69.25, 3.31 and -62.06 and their selection factors were 1.91, 1.75, 2.13 respectively. Selection parameters of master curve '$\alpha$' and '$\beta$' were 3.16 and -6.24 respectively, and (L/M) sub(50) was 1.98. The optimum mesh size was 71mm.

• Journal of the Korean Society for Marine Environment & Energy
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• v.12 no.2
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• pp.84-90
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• 2009

We incubated marine columnar sediments at $25^{\circ}C$ for 230 days to simulate the responses of phosphorus in the sediment which was exposed to freshwater. The incubation was composed of three different treatments (FW: freshwater, FWA: freshwater under anoxic condition, and SW: seawater as a Control). Six particulate fractions of phosphorus in sediment were obtained through sequential extraction and, for comparison, phosphate concentrations in porewater and superlying water were also determined. After the incubation, evidently higher concentrations of phosphate were found in FW and FWA compared to SW. Mass extinction of living organisms in marine sediment from freshwater shock and consequent decay of their corps probably contributed such high phosphate spike in the overlying water. Higher concentrations of BD-P(lron-bound P) were found in FW compared to SW. After exposure to the freshwater, we could determine that penetration depth of dissolved oxygen in marine sediment will be deeper. A result of increases of ferrous compounds in freshwater where contained less sulfide has been obtained. Because of these phenomena, BD-P was increased in FW. On the contrary, BD-P was decreased in FWA since poor dissolved oxygen concentration. In FWA, total amount of Leachable P(SUM of LOP) has been remarkably increased through the experiment, which strongly suggested the easy conversion of the leachable P into reactive P. This experiment has shown that most of diverse P species in marine sediment were leachable under freshwater and low oxygen condition. Therefore reclamation of natural tidalfalt and consequent freshwater introduction seems to trigger the conversion of diverse P-species to leachable P in the marine sediments, which will exert high benthic load of phosphate to the overlying water.

• Journal of Life Science
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• v.16 no.6
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• pp.904-910
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• 2006

Dendritic cells (DCs) are the only antigen presenting cells (APCs) capable of initiating immune responses, which is crucial for priming the specific cytotoxic T lymphocyte (CTL) response and tumor immunity. Upon activation by DCs, CD4+ helper T cells can cross-prime CD8+ CTLs via IL-12. However, recently activated DCs were described to prime in vitro strong T helper cell type 1 $(Th_1)$ responses, whereas at later time points, they preferentially prime $Th_2$ cells. Therfore, we examined in this study the optimum kinetic state of DCs activation impacted on in vivo priming of tumor-specific CTLs by using ovalbumin (OVA) tumor antigen model. Bone-marrow-derived DCs showed an appropriate expression of surface MHC and costimulatory molecules after 6 or 7-day differentiation. The 6-day differentiated DCs pulsed with OVA antigen for 8 h (8-h DC) and followed by restimulation with LPS for 24 h maintained high interleukin (IL)-12 production potential, accompanying the decreased level in their secretion by delayed re-exposure time to LPS. Furthermore, immunization with 8-h DC induced higher intracellular $interferon(IFN)-{\gamma}+/CD8+T$ cells and elicited more powerful cytotoxicity of splenocytes to EG7 cells, a clone of EL4 cells transfected with an OVA cDNA, than immunization with 24-h DC. In the animal study for the evaluation of therapeutic or protective antitumor immunity, immunization with 8-h DC induced an effective antitumor immunity against tumor of EG7 cells and completely protected mice from tumor formation and prolonged survival, respectively. The most commonly used and clinically applied DC-based vaccine is based on in vitro antigen loading for 24 h. However, our data indicated that antigen stimulation over 8 h decreased antitumor immunity with functional exhaustion of DCs, and that the 8-h DC would be an optimum activation state impacted on in vivo priming of tumor-specific CTLs and subsequently lead to induction of strong antitumor immunity.

• Journal of Life Science
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• v.21 no.11
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• pp.1518-1525
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• 2011

Sorafenib is the only approved systemic, therapeutic agent for hepatocellular carcinoma (HCC). The use of Ginseng Extract (GE) in cancer patients is growing worldwide; however, drug interaction between sorafenib and GE has not been illuminated. Four different human cancer cell lines including HepG2 were used and immunocompetent mice were implanted subcutaneously with a mouse HCC cell line. Treatment with low dose GE stimulated cell growth, while a high dose inhibited growth. pERK (phosphorylation of extracellular signal-regulated kinase) was concomitantly increased and decreased respective of different doses of GE. Antitumoral effect of sorafenib decreased in non-proliferating phase cells but was sensitized after low dose GE (LDG) treatment. PD98059 (ERK phosphorylation inhibitor) efficiently blocked ERK phosphorylation, resulting in loss of sorafenib sensitization even after LDG treatment. In the HCC mouse model, LDG alone slightly increased tumor size while sorafenib alone significantly decreased it. However, a combination of LDG and sorafenib significantly decreased tumor size compared with sorafenib alone. Increase of pERK was observed in some normal mice organs and mild inflammatory change was observed in some of these organs, suggesting pERK activation by LDG may cause unexpected toxicity in normal cells. GE, dose-dependently, induced stimulation or inhibition in some human cancer cell lines. Combinational use of GE and sorafenib possibly potentiated an antitumoral response to sorafenib. pERK level has been provided as a potential predictive marker for sorafenib. Our result may suggest GE's dual effects in relation to pERK level in HCC cancer cell lines, and that certain doses of GE can sensitize sorafenib.

• Journal of Life Science
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• v.21 no.3
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• pp.375-384
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• 2011

This study was performed to evaluate the antioxidant and antimicrobial activity of methanol extract from Rosmarinus officinalis L. and its fractions. The ethyl acetate fraction of rosemary had a higher antioxidant activity in both DPPH ($3.22\;{\mu}g/ml$) and ABTS ($5.05\;{\mu}g/ml$) compared to other extracts and fractions. Based on the results of the FRAP assay, the ethyl acetate fraction of rosemary showed a value of $5.9{\pm}0.3\;{\mu}M/{\mu}g$, and buthanol fraction and rosmarinic acid exhibited values of $4.8{\pm}0.2\;{\mu}M/{\mu}g$ and $5.1{\pm}0.1\;{\mu}M/{\mu}M$, respectively. Measurements of the antimicrobial activities of the extracts, fraction against gram positive, negative bacteria revealed that the methanol extract, hexane, ethyl acetate, and chloroform fraction of rosemary caused Staphylococcus aureus and Escherichia coli to form clear zones greater than 12 mm. Furthermore, the methanol extract and chloroform fraction showed high antibacterial activity, with inhibition zone exceeding 13 mm. The methanol extract and chloroform fraction of rosemary had broad antimicrobial spectrums and low MIC values. Therefore, methanol extracts of rosemary could serve as potential antibacterial agents to inhibit pathogen growth in food and hand sanitizers.

• Journal of Life Science
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• v.21 no.7
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• pp.953-960
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• 2011

Necrosis is characterized by the cell membrane rupture and release of the cellular contents, including high-mobility group box 1 protein (HMGB1), into the extracellular microenvironment. HMGB1 acts as a transcriptional regulator in nuclei, but exerts a pro-inflammatory and tumor-promoting cytokine activity when released into the extracellular space. Its overexpression is associated with tumor progression and chemoresistance. Thus, HMGB1 acts as a clinically important molecule in tumor biology. In this study, we examined whether HMGB1 affects cell death induced by anti-cancer drugs. Here we show that HMGB1 prevented cisplatin (alkylating agent)-induced apoptosis and switched the cell fate to necrosis in MCF-7, MDA-MB231, and MDA-MB361 cells. Similar apoptosis-to-necrosis switch effects of HMGB1 were observed in cells treated with 4-HC, another alkylating agent. In contrast, HMGB1 did not exert any significant effects on docetaxel (DOC)-induced apoptosis in MCF-7 cells. We also show that cisplatin-induced apoptosis was switched to necrosis in MCF-7 multicellular tumor spheroids (MTS) that were cultured for 8 days and had necrotic cores, but DOC-induced apoptosis was prevented without the apoptosis-to-necrosis switch. Finally, the levels of RAGE, a receptor of HMGB1, were increased with extended culture of MTS. These findings demonstrate that HMGB1 switches alkylating agent-induced apoptosis to necrosis, suggesting that the strategy to prevent necrosis occurring as an undesirable action of alkylating agent-based chemotherapy should be delineated to improve the efficacy of chemotherapy for cancer.