• Journal of Gastric Cancer
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• 제17권4호
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• pp.295-305
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• 2017

Purpose: We previously found that the histone methyltransferase suppressor of variegation, enhancer of zeste, trithorax and myeloid-nervy-deformed epidermal autoregulatory factor-1 domain-containing protein 3 (SMYD3) is a potential independent predictive factor or prognostic factor for overall survival in gastric cancer patients, but its roles seem to differ from those in other cancers. Therefore, in this study, the detailed functions of SMYD3 in cell proliferation and migration in gastric cancer were examined. Materials and Methods: SMYD3 was overexpressed or suppressed by transfection with an expression plasmid or siRNA, and a wound healing migration assay and Transwell assay were performed to detect the migration and invasion ability of gastric cancer cells. Additionally, an MTT assay and clonogenic assay were performed to evaluate cell proliferation, and a cell cycle analysis was performed by propidium iodide staining. Furthermore, the expression of genes implicated in the ataxia telangiectasia mutated (ATM) pathway and proteins involved in cell cycle regulation were detected by polymerase chain reaction and western blot analyses. Results: Compared with control cells, gastric cancer cells transfected with si-SMYD3 showed lower migration and invasion abilities (P<0.05), and the absence of SMYD3 halted cells in G2/M phase and activated the ATM pathway. Furthermore, the opposite patterns were observed when SMYD3 was elevated in normal gastric cells. Conclusions: To the best of our knowledge, this study provides the first evidence that the absence of SMYD3 could inhibit the migration, invasion, and proliferation of gastric cancer cells and halt cells in G2/M phase via the ATM-CHK2/p53-Cdc25C pathway. These findings indicated that SMYD3 plays crucial roles in the proliferation, migration, and invasion of gastric cancer cells and may be a useful therapeutic target in human gastric carcinomas.

• BMB Reports
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• 제37권4호
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• pp.402-407
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• 2004

A human gene T-complex protein 10 like (TCP10L) was cloned in our lab. A previous study showed that it expressed specifically in the liver and testis. A transcription experiment revealed that TCP10L was a transcription factor with transcription inhibition activity. In this study, the human MAD4 was identified to interact with TCP10L by a yeast two-hybrid screen. This finding was confirmed by immunoprecipitation and subcellular localization experiments. As MAD4 is a member of the MAD family, which antagonizes the functions of MYC and promotes cell differentiation, the biological function of the interaction between TCP10L and MAD4 may be to maintain the differentiation state in liver cells. Also, we propose that the up-regulation of Myc is caused by the down-regulation of TCP10L in human hepatocarcinomas.

• 한국전자거래학회지
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• 제18권3호
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• pp.125-142
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• 2013

IT비즈니스의 전략적 연계가 강화됨에 따라 금융서비스에서 IT의존도는 높아지고 있다. 안전하고 신뢰된 금융서비스를 제공하기 위해서는 지속적인 정보보호활동을 수행해야 하며, 이에 관한 조직의 정보보호 업무성과 측정은 의사결정 및 경영지원 측면에서 유용하다. 본 논문은 정보보호관리체계(K-ISMS)와 금융IT 부문 정보보호 업무 모범규준의 평가기준을 기반으로 핵심성공요인(CSF, Critical Success Factor)과 핵심성과지표(KPI, Key Performance Indicator)를 도출한다. 이는 정보보호정책 준수에 유의한 영향을 주는 핵심성과지표를 판별하는 논리적 근거를 제공하며, 금융IT 정보보호 역량을 강화하기 위한 정책을 수립하기 위한 기초자료로 활용할 수 있다.

• Animal Bioscience
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• 제34권8호
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• pp.1403-1414
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• 2021

Objective: This study explored the mechanism of the Kelch-like erythroid cell-derived protein with CNC homology-associated protein 1 (Keap1)-nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway under conditions of zearalenone (ZEA)-induced oxidative stress in the duodenum of post-weaning gilts. Methods: Forty post-weaning gilts were randomly allocated to four groups and fed diets supplemented with 0, 0.5, 1.0, or 1.5 mg/kg ZEA. Results: The results showed significant reductions in the activity of the antioxidant enzymes total superoxide dismutase and glutathione peroxidase and increases the malondialdehyde content with increasing concentrations of dietary ZEA. Immunohistochemical analysis supported these findings by showing a significantly increased expression of Nrf2 and glutathione peroxidase 1 (GPX1) with increasing concentrations of ZEA. The relative mRNA and protein expression of Nrf2, GPX1 increased linearly (p<0.05) and quadratically (p<0.05), which was consistent with the immunohistochemical results. The relative mRNA expression of Keap1 decreased linearly (p<0.05) and quadratically (p<0.05) in the duodenum as the ZEA concentration increased in the diet. The relative mRNA expression of modifier subunit of glutamate-cysteine ligase (GCLM) increased quadratically (p<0.05) in all ZEA treatment groups and the relative mRNA expression of quinone oxidoreductase 1 (NQO1) catalytic subunit of glutamate-cysteine ligase decreased linearly (p<0.05) and quadratically (p<0.05) in the ZEA1.0 group and ZEA1.5 group. The relative protein expression of Keap1 and GCLM decreased quadratically (p<0.05) in the duodenum as the ZEA concentration increased in the diet, respectively. The relative protein expression of NQO1 increased linearly (p<0.05) and quadratically (p<0.05) in all ZEA treatment groups in the duodenum. Conclusion: These findings suggest that ZEA regulates the expression of key factors of the Keap1-Nrf2 signaling pathway in the duodenum, which enables resistance to ZEA-induced oxidative stress. Further studies are needed to examine the effects of ZEA induced oxidative stress on other tissues and organs in post-weaning gilts.

• 한국정보통신학회논문지
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• 제21권7호
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• pp.1411-1419
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• 2017

PKI(Public Key Infrastructure) 인증은 개인키 소지(possession)와 개인키 보호 패스워드 지식(knowledge)이라는 2 요소 인증(2 factor authentication) 능력과 안전한 공개키 암호 프로토콜을 통해 인터넷 거래의 신뢰 인프라 구축에 많은 기여를 해왔다. 하나의 인증서로 모든 PKI 사이트를 접근할 수 있는 점도 PKI 인증의 활성화에 기여하였다. 그럼에도 불구하고 인증서 인프라 구축 비용, 인증서 관리에 따른 사용자 불편함, 그리고 개인키 보호 패스워드 관리의 어려움 등에 따른 여러 가지 문제점들이 노출되어 왔다. 최근에 주목받고 있는 FIDO(Fast IDentity Online) 인증은 PKI 인증과 같이 공개키 암호 프로토콜에 기초한 강력한 인증 서비스를 제공하면서도 사용자별 인증서 발급이 불필요하고, 생체 인증 등과 결합하여 안전하고 편리한 인증 서비스 제공을 추구하고 있다. 본 논문은 PKI 인증과 FIDO 인증의 동작 방식을 구체적으로 비교하여 각각의 장단점을 분석하고, 그에 따른 각각의 응용 분야를 제시하는 데 목적이 있다.

• Animal Bioscience
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• 제35권4호
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• pp.517-526
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• 2022

Objective: The growth differentiation factor 8 (GDF8) gene plays a key role in bone formation, resorption, and skeletal muscle development in mammals. Here, we studied the genetic variants of GDF8 and their contribution to body conformation traits in Chinese Dabieshan cattle. Methods: Single nucleotide polymorphisms (SNPs) were identified in the bovine GDF8 gene by DNA sequencing. Phylogenetic analysis, motif analysis, and genetic diversity analysis were conducted using bioinformatics software. Association analysis between five SNPs, haplotype combinations, and body conformation traits was conducted in 380 individuals. Results: The GDF8 was highly conserved in seven species, and the GDF8 sequence of cattle was most similar to the sequences of sheep and goat based on the phylogenetic analysis. The motif analysis showed that there were 12 significant motifs in GDF8. Genetic diversity analysis indicated that the polymorphism information content of the five studied SNPs was within 0.25 to 0.5. Haplotype analysis revealed a total of 12 different haplotypes and those with a frequency of <0.05 were excluded. Linkage disequilibrium analysis showed a strong linkage (r²>0.330) between the following SNPs: g.5070C>A, g.5076T>C, and g.5148A>C. Association analysis indicated these five SNPs were associated with some of the body conformation traits (p<0.05), and the animals with haplotype combination H1H1 (-GGGG CCTTAA-) had greater wither height, hip height, heart girth, abdominal girth, and pin bone width than the other (p<0.05) Dabieshan cattle. Conclusion: Overall, our results indicate that the genetic variants of GDF8 affected the body conformation traits of Chinese Dabieshan cattle, and the GDF8 gene could make a strong candidate gene in Dabieshan cattle breeding programs.

• Geomechanics and Engineering
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• 제29권5호
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• pp.567-582
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• 2022

In the present study, a series of physical experiments and numerical simulations were conducted to investigate the effects of mode I and mixed-mode I/II cracks on the fracture modes and stability of roadway tunnel models. The experiments and simulations incorporated different inclination angle flaws under both static and dynamic loads. The quasi-static and dynamic testing were conducted by using an electro-hydraulic servo control device and drop weight impact system (DWIS), and the failure process was simulated by using rock failure process analysis (RFPA) and AUTODYN software. The stress intensity factor was also calculated to evaluate the stability of the flawed roadway tunnel models by using ABAQUS software. According to comparisons between the test and numerical results, it is observed that for flawed roadways with a single radical crack and inclination angle of 45°, the static and dynamic stability are the lowest relative to other angles of fractured rock masses. For mixed-mode I/II cracks in flawed roadway tunnel models under dynamic loading, a wing crack is produced and the pre-existing cracks increase the stress concentration factor in the right part of the specimen, but this factor will not be larger than the maximum principal stress region in the roadway tunnel models. Additionally, damage to the sidewalls will be involved in the flawed roadway tunnel models under static loads.

• Journal of Microbiology
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• 제43권6호
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• pp.529-536
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• 2005

Viral infection causes stress to the endoplasmic reticulum (ER). The response to endoplasmic reticulum stress, known as the unfolded protein response (UPR), is designed to eliminate misfolded proteins and allow the cell to recover. The role of hepatitis C virus (HCV) non-structural protein NS4B, a component of the HCV replicons that induce UPR, is incompletely understood. We demonstrate that HCV NS4B could induce activating transcription factor (ATF6) and inositol-requiring enzyme 1 (IRE1), to favor the HCV subreplicon and HCV viral replication. HCV NS4B activated the IRE1 pathway, as indicated by splicing of X box-binding protein (Xbp-1) mRNA. However, transcriptional activation of the XBP-1 target gene, EDEM (ER degradation-enhancing $\alpha-mannosidase-like$ protein, a protein degradation factor), was inhibited. These results imply that NS4B might induce UPR through ATF6 and IRE1-XBP1 pathways, but might also modify the outcome to benefit HCV or HCV subreplicon replication.

• 품질경영학회지
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• 제31권1호
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• pp.123-131
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• 2003

This paper considers a six sigma project for reducing the defects rate of the welding process in manufacturing firms. The project follows a disciplined process of five macro phases. define, measure, analyze, improve and control(DMAIC). The need of customers is used to identify critical to quality(CTQ) of project. And a process map is used to identify process input factors of CTQ. Four key process input factors are selected by using an input factor evaluation of teams; an interval of welding, an abrasion, an electric current and a moving freely. DOE is utilized for finding the optimal process conditions of the three key process input factors. Another one key input factor improved to welding machine. The six sigma level of defects rate becomes a 2.01 from a 1.61 at the beginning of the project.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제9권9호
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• pp.3685-3700
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• 2015

To avoid influences caused by pose, illumination and facial expression variations, we propose a robust three-step algorithm based on ASM and POEM for facial landmark localization. Firstly, Model Selection Factor is utilized to achieve a pose-free initialized shape. Then, we use the global shape model of ASM to describe the whole face and the texture model POEM to adjust the position of each landmark. Thirdly, a second localization is presented to discriminatively refine the subtle shape variation for some organs and contours. Experiments are conducted in four main face datasets, and the results demonstrate that the proposed method accurately localizes facial landmarks and outperforms other state-of-the-art methods.