• Asian-Australasian Journal of Animal Sciences
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• v.33 no.9
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• pp.1507-1519
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• 2020

Objective: The current research was aimed to profile the transcriptomic picture of the peripheral blood lymphocytes (PBLs) associated with immunity in Chinese Holsteins supplemented orally with coated folic acid during the periparturient period. Methods: The total of 123 perinatal cows were selected for this study and divided into three groups; group A (n = 41, 240 mg/500 kg cow/d), group B (n = 40, 120 mg/500 kg cow/d) and group C (n = 42, 0 mg/cow/d) based on the quantity of folic acid fed. Three samples of PBLs were selected from each folic acid treated group (high, low, and control) and RNA sequencing method was carried out for transcriptomic analysis. Results: The analysis revealed that a higher number of genes and pathways were regulated in response to high and low folic acid supplementation compared to the controls. We reported the novel pathways tumor necrosis factor (TNF) signaling, antigen processing and presentation, Staphylococcus aureus infection and nuclear factor (NF)-kappa B signaling pathways) and the key genes (e.g. C-X-C motif chemokine ligand 10, TNF receptor superfamily member 1A, cluster difference 4, major histocompatibility complex, class II, DQ beta, NF-kappa-B inhibitor alpha, and TNF superfamily 13) having great importance in immunity and anti-inflammation in the periparturient cows in response to coated folic acid treatment. Conclusion: Collectively, our study profiled first-time transcriptomic analysis of bovine lymphocytes and compared the involved cytokines, genes, and pathways between high vs control and low vs control. Our data suggest that the low folic acid supplementation (120 mg/500 kg) could be a good choice to boost appropriate immunity and anti-inflammation as well as might being applied to the health improvement of perinatal dairy cows.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.10
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• pp.1490-1495
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• 2007

Insulin-like growth factor binding protein-4 (IGFBP-4) is a member of the IGF super family, and regulates the action of IGFs. The polymorphism of porcine IGFBP-4 gene in 17 pig breeds (total n = 570) was detected by PCR-SSCP, and alleles A and B were detected. In these pig breeds, it was found that exotic pig breeds carried high frequencies of allele A, while Chinese native pig breeds carried high frequencies of allele B. The role of porcine IGFBP-4 was investigated in 172 F2 offspring of a $Lantang{\times}Lantang $ population. Forty eight growth traits were recorded for analyzing the association between IGFBP-4 gene polymorphism and quantitative performance traits. In this resource family, pigs with AA genotype had higher fore-body weight, bone weight of mid-body, bone weight of rear-body, fore-leg weight and rear-leg weight than those pigs with BB genotype (p<0.05); while pigs which carried BB genotype had higher back-fat thickness at C point and lard weight than those pigs with AA genotype (p<0.05); pigs with AA genotype had higher body weight than those with BB genotype; for meat quality traits, pigs with AA genotype had higher meat color than those of BB genotype (p<0.01), and pigs with BB genotype had higher marbling than those of AA and AB genotypes (p<0.01 and p<0.05, respectively).Based on these results, it is necessary to do more studies on IGFBP-4 before using the IGFBP-4 locus for the application of marker-assisted selection programs.

• Advances in concrete construction
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• v.9 no.4
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• pp.413-421
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• 2020

For steel-concrete girders made composite using shear studs, initial damage on studs induced by weld defect, unexpected overloading, fatigue and others might degrade the service performance and even threaten the structural safety. This paper conducted a numerical study to investigate the static behavior of damaged stud shear connectors that were embedded in ultra high performance concrete (UHPC). Parameters included damage degree and damage location. The material nonlinear behavior was characterized by multi-linear stress-strain relationship and damage plasticity model. The results indicated that the shear strength was not sensitive to the damage degree when the damage occurred at 2/3d (d is the stud diameter) from the stud root. An increased stud area would be engaged in resisting shear force as the distance of damage location from stud root increased and the failure section becomes inclined, resulting in a less reduction in the shear strength and shear stiffness. The reduction factor was proposed to consider the degradation of the shear strength of the damaged stud. The reduction factor can be calculated using two approaches: a linear relationship and a square relationship with the damage degree corresponding to the shear strength dominated by the section area and the nominal diameter of the damaged stud. It was found that the proposed method is preferred to predict the shear strength of a stud with initial damage.

• Journal of Microbiology and Biotechnology
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• v.23 no.12
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• pp.1737-1746
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• 2013

IbMYB1, a transcription factor (TF) for R2R3-type MYB TFs, is a key regulator of anthocyanin biosynthesis during storage of sweet potatoes. Anthocyanins provide important antioxidants of nutritional value to humans, and also protect plants from oxidative stress. This study aimed to increase transgenic potatoes' (Solanum tuberosum cv. LongShu No.3) tolerance to environmental stress and enhance their nutritional value. Transgenic potato plants expressing IbMYB1 genes under the control of an oxidative stress-inducible peroxidase (SWPA2) promoter (referred to as SM plants) were successfully generated through Agrobacterium-mediated transformation. Two representative transgenic SM5 and SM12 lines were evaluated for enhanced tolerance to salinity, UV-B rays, and drought conditions. Following treatment of 100 mM NaCl, seedlings of SM5 and SM12 lines showed less root damage and more shoot growth than control lines expressing only an empty vector. Transgenic potato plants in pots treated with 400 mM NaCl showed high amounts of secondary metabolites, including phenols, anthocyanins, and flavonoids, compared with control plants. After treatment of 400 mM NaCl, transgenic potato plants also showed high DDPH radical scavenging activity and high PS II photochemical efficiency compared with the control line. Furthermore, following treatment of NaCl, UV-B, and drought stress, the expression levels of IbMYB1 and several structural genes in the flavonoid biosynthesis such as CHS, DFR, and ANS in transgenic plants were found to be correlated with plant phenotype. The results suggest that enhanced IbMYB1 expression affects secondary metabolism, which leads to improved tolerance ability in transgenic potatoes.

• Korean Journal of Environment and Ecology
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• v.24 no.1
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• pp.78-86
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• 2010

The classification of biotope type in major Korean cities was made based on the land use concept rather than the ecological concept of the land as the habitat of biological communities. Therefore, biotope type need to be reclassified according to ecological concerns and regional characteristics. This study attempts to clearly define various critical concepts regarding the classification of biotope type, such as classification hierarchy, classification criteria, classification factor, classification indicator, classification key, and classification standard. Furthermore, it also attempts to suggest the ways to improve the classification system of biotope type by sampling the cases of major Korean cities. The classification system of biotope type is required to have a coherent system that provides basic guidelines, standards and hierarchy with regard to biotic, abiotic and anthropotic factors, as well as classification indicators and classification keys.

• Journal of Power Electronics
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• v.17 no.3
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• pp.766-776
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• 2017

In modern power systems, high penetration of distributed generators (DGs) results in high stress on system stability. Apart from the intermittent nature of DGs, most DGs do not contribute inertia or damping to systems. As a result, a new control method named virtual synchronous machine (VSM) was proposed, which brought new characteristics to inverters such as synchronous machines (SMs). In addition, different active power droop controls for VSMs are being proposed in literatures. However, they are quite different in terms of their dynamic characteristics despite of the similar control laws. In this paper, mathematical models of a VSM adopting different active power droop controls are built and analyzed. The dynamic performance of the VSM output active power and virtual rotor angular frequency are presented for different models. The influences of the damping factor and droop coefficient on the VSM dynamic behaviors are also investigated in detail. Finally, the theoretical analysis is verified by simulations and experimental results.

• Genomics & Informatics
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• v.20 no.3
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• pp.26.1-26.19
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• 2022

Diabetes and its related complications are associated with long term damage and failure of various organ systems. The microvascular complications of diabetes considered in this study are diabetic retinopathy, diabetic neuropathy, and diabetic nephropathy. The aim is to identify the weighted co-expressed and differentially expressed genes (DEGs), major pathways, and their miRNA, transcription factors (TFs) and drugs interacting in all the three conditions. The primary goal is to identify vital DEGs in all the three conditions. The overlapped five genes (AKT1, NFKB1, MAPK3, PDPK1, and TNF) from the DEGs and the co-expressed genes were defined as key genes, which differentially expressed in all the three cases. Then the protein-protein interaction network and gene set linkage analysis (GSLA) of key genes was performed. GSLA, gene ontology, and pathway enrichment analysis of the key genes elucidates nine major pathways in diabetes. Subsequently, we constructed the miRNA-gene and transcription factor-gene regulatory network of the five gene of interest in the nine major pathways were studied. hsa-mir-34a-5p, a major miRNA that interacted with all the five genes. RELA, FOXO3, PDX1, and SREBF1 were the TFs interacting with the major five gene of interest. Finally, drug-gene interaction network elucidates five potential drugs to treat the genes of interest. This research reveals biomarker genes, miRNA, TFs, and therapeutic drugs in the key signaling pathways, which may help us, understand the processes of all three secondary microvascular problems and aid in disease detection and management.

• Magazine of the Korean Society of Agricultural Engineers
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• v.45 no.3
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• pp.29-40
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• 2003

The objective of the paper is to compute the half-month rainfall-runoff erosivity factor for revised universal soil loss equation (RUSLE). RUSLE is being used to develop soil conservation programs and identify optimum management practices. Rainfall-runoff erosivity factor (R) is a key input parameter to RUSLE. Rainfall-runoff erosivity factor has been calculated for twenty six stations from the nationwide rainfall data from 1973 to 2002 in south Korea. The average annual Rainfall-runoff erosivity factor at the analyzed stations Is between 3,130 and 10,476 (MJ/ha)ㆍ(mm/h). According to the computation of the half-month Rainfall-runoff erosivity factor for locations, 66-85% of the average annual R value has occurred during the summer months, June-August. The half-month R values from this study can be used for RUSLE.

• Journal of Electrical Engineering and Technology
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• v.12 no.1
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• pp.385-395
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• 2017

A new digitized decoupled dual-axis micro dynamically tuned gyroscope with three equilibrium rings (TMDTG) is proposed which can eliminate the constant torque disturbance (CTD) caused by the double rotation frequency of a driving shaft with a micro dynamically tuned gyroscope with one equilibrium ring (MDTG). A mechanical and kinematic model of the TMDTG is theoretically analyzed and the structure parameters are optimized in ANSYS to demonstrate reliability. By adjusting the thickness of each equilibrium ring, the CTD can be eliminated. The digitized model of the TMDTG system is then simulated and examined using MATLAB. Finally, a digitized prototype based on FPGA is created. The gyroscope can be dynamically tuned by adjusting feedback voltage. Experimental results show the TMDTG has good performance with a scale factor of $283LSB/^{\circ}/s$ in X-axis and $220LSB/^{\circ}/s$ in Y-axis, respectively. The scale factor non-linearity is 0.09% in X-axis and 0.13% in Y-axis. Results from analytical models, simulations, and experiments demonstrate the feasibility of the proposed TMDTG.

• BMB Reports
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• v.40 no.4
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• pp.547-553
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• 2007

Many motifs along the 1.2 kb myostatin promoter (MSTNpro) in sheep have been found by the MatInspecter program in our recent study. To further verify the role of the motifs and better understand the transcriptional regulation mechanism of the myostatin gene in sheep, the reporter gene EGFP (enhanced green fluorescent protein) was selected and the wild-type (W) vector MSTNPro$^W$-EGFP or motif-mutational (M) vector MSTNPro$^M$-EGFP were constructed. The transcriptional regulation activities were analyzed by detecting the fluorescence strength of EGFP in C2C12 myoblasts transfected with the vectors. The results showed that E-box (E) 3, E4, E5 and E7, particularly E3, E5 and E7, had important effects on the activity of the 1.2 kb sheep myostatin promoter. In addition, we also detected several other important motifs such as MTBF (muscle-specific Mt binding factor), MEF2 (myocyte enhancer factor 2), GRE (glucocorticoid response elements) and PRE (progesterone response elements) along the sheep myostatin promoter by the mutational analysis.