• Title/Summary/Keyword: Key Technology

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Size control of Co-doped ZnO rods by changing the solvent

  • Zhao, Jing;Yan, Xiaoqin;Lei, Yang;Zhao, Yanguang;Huang, Yunhua;Zhang, Yue
    • Advances in materials Research
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    • v.1 no.1
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    • pp.75-81
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    • 2012
  • In this work, the Co-doped ZnO rods were prepared by the hydrothermal method. The size of these rods can be changed from micro-size to nano-size by using different solutions during the preparation. The results of transmission electron microscopy (TEM) and selected area electron diffraction (SAED) showed that the as-prepared nano-sized Co-doped rods have single-crystal structure. The polarized Raman experiments were presented on an individual micro-sized Co-doped ZnO rod in the $X(YY)\vec{X}$, $X(ZY)\vec{X}$ and $X(ZZ)\vec{X}$ configurations, the results of polarized Raman indicated that these rods are crystallized and their growth direction is parallel to c-axis.

Obliquely incident earthquake for soil-structure interaction in layered half space

  • Zhao, Mi;Gao, Zhidong;Wang, Litao;Du, Xiuli;Huang, Jingqi;Li, Yang
    • Earthquakes and Structures
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    • v.13 no.6
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    • pp.573-588
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    • 2017
  • The earthquake input is required when the soil-structure interaction (SSI) analysis is performed by the direct finite element method. In this paper, the earthquake is considered as the obliquely incident plane body wave arising from the truncated linearly elastic layered half space. An earthquake input method is developed for the time-domain three-dimensional SSI analysis. It consists of a new site response analysis method for free field and the viscous-spring artificial boundary condition for scattered field. The proposed earthquake input method can be implemented in the process of building finite element model of commercial software. It can result in the highly accurate solution by using a relatively small SSI model. The initial condition is considered for the nonlinear SSI analysis. The Daikai subway station is analyzed as an example. The effectiveness of the proposed earthquake input method is verified. The effect of the obliquely incident earthquake is studied.

Numerical study on tensioned membrane structures under impact load

  • Zhang, Yingying;Zhao, Yushuai;Zhang, Mingyue;Zhou, Yi;Zhang, Qilin
    • Structural Engineering and Mechanics
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    • v.71 no.2
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    • pp.109-118
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    • 2019
  • This paper presents the numerical simulation of membrane structure under impact load. Firstly, the numerical simulation model is validated by comparing with the test in Hao's research. Then, the effects of the shape of the projectile, the membrane prestress and the initial impact speed, are investigated for studying the dynamic response and failure mechanism, based on the membrane displacement, projectile acceleration and kinetic energy. Finally, the results show that the initial speed and the punch shape are related with the loss of kinetic energy of projectiles. Meanwhile, the membrane prestress is an important factor that affects the energy dissipation capacity and the impact resistance of membrane structures.

Identification and Characterization of the Replication Region of Virulence Plasmid pEIB202 in Edwardsiella piscicida

  • Chang, Xinyue;Teng, Chengli;Wu, Haizhen;Ye, Jiang;Wang, Qiyao;Zhang, Huizhan
    • Journal of Microbiology and Biotechnology
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    • v.29 no.8
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    • pp.1273-1280
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    • 2019
  • Edwardsiella piscicida is the causative agent of edwardsiellosis, which has caused enormous economic losses worldwide. In our previous research, an attenuated live vaccine known as WED and based on the virulent strain E. piscicida EIB202 can effectively protect turbots against edwardsiellosis via intraperitoneal injection, while vaccination by immersion exhibits a weaker effect. During the development of the immersion vaccine, we surprisingly found the counts of ${\Delta}pEIB202/EIB202$ colonized on zebrafish were 100 times lower than those of EIB202. However, pEIB202 carries 53 predicted ORFs and has several copies in E. piscicida EIB202, impeding the study of its function. Thus, the replication region is located at a 1,980 bp fragment (from 18,837 to 20,816 bp), containing a transcriptional repressor and a replication protein. Moreover, the minimal replication plasmid, named pRep-q77, has low copies in both E. coli and E. piscicida, but is more stable in E. piscicida than in E. coli. This work lays a foundation for further examination of the function of the virulence plasmid pEIB202.

Analyzing the Influence of Spatial Sampling Rate on Three-dimensional Temperature-field Reconstruction

  • Shenxiang Feng;Xiaojian Hao;Tong Wei;Xiaodong Huang;Pan Pei;Chenyang Xu
    • Current Optics and Photonics
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    • v.8 no.3
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    • pp.246-258
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    • 2024
  • In aerospace and energy engineering, the reconstruction of three-dimensional (3D) temperature distributions is crucial. Traditional methods like algebraic iterative reconstruction and filtered back-projection depend on voxel division for resolution. Our algorithm, blending deep learning with computer graphics rendering, converts 2D projections into light rays for uniform sampling, using a fully connected neural network to depict the 3D temperature field. Although effective in capturing internal details, it demands multiple cameras for varied angle projections, increasing cost and computational needs. We assess the impact of camera number on reconstruction accuracy and efficiency, conducting butane-flame simulations with different camera setups (6 to 18 cameras). The results show improved accuracy with more cameras, with 12 cameras achieving optimal computational efficiency (1.263) and low error rates. Verification experiments with 9, 12, and 15 cameras, using thermocouples, confirm that the 12-camera setup as the best, balancing efficiency and accuracy. This offers a feasible, cost-effective solution for real-world applications like engine testing and environmental monitoring, improving accuracy and resource management in temperature measurement.

Knockdown of endogenous SKIP gene enhanced insulin-induced glycogen synthesis signaling in differentiating C2C12 myoblasts

  • Xiong, Qi;Deng, Chang-Yan;Chai, Jin;Jiang, Si-Wen;Xiong, Yuan-Zhu;Li, Feng-E;Zheng, Rong
    • BMB Reports
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    • v.42 no.2
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    • pp.119-124
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    • 2009
  • PI(3,4,5)$P_3$ produced by the activated PI3-kinase is a key lipid second messenger in cell signaling downstream of insulin. Skeletal muscle and kidney-enriched inositol phosphatase (SKIP) identified as a 5'-inositol phosphatase that hydrolyzes PI(3,4,5) $P_3$ to PI(3,4)$P_2$, negatively regulates the insulin-induced glycogen synthesis in skeletal muscle. However the mechanism by which this occurs remains unclear. To elucidate the function of SKIP in glycogen synthesis, we employed RNAi techniques to knockdown the SKIP gene in differentiating C2C12 myoblasts. Insulininduced phosphorylation of Akt (protein kinase B) and GSK-3$\beta$ (Glycogen synthase kinase), subsequent dephosphorylation of glycogen synthase and glycogen synthesis were increased by inhibiting the expression of SKIP, whereas the insulin-induced glycogen synthesis was decreased by overexpression of WT-SKIP. Our results suggest that SKIP plays a negative regulatory role in Akt/ GSK-3$\beta$/GS (glycogen synthase) pathway leading to glycogen synthesis in myocytes.

A Novel Method for Survivability Test Based on End Nodes in Large Scale Network

  • Ming, Liang;Zhao, Gang;Wang, Dongxia;Huang, Minhuan;Li, Xiang;Miao, Qing;Xu, Fei
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.9 no.2
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    • pp.620-636
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    • 2015
  • Survivability is a necessary property of network system in disturbed environment. Recovery ability is a key actor of survivability. This paper concludes network survivability into a novel composite metric, i.e. Network Recovery Degree (NRD). In order to measure this metric in quantity, a concept of Source-Destination Pair (SD Pair), is created to abstract end-to-end activity based on end nodes in network, and the quality of SD Pair is also used to describe network performance, such as connectivity, quality of service, link degree, and so on. After that, a Survivability Test method in large scale Network based on SD pairs, called STNSD, is provided. How to select SD Pairs effectively in large scale network is also provided. We set up simulation environment to validate the test method in a severe destroy scenario and evaluate the method scalability in different large scale network scenarios. Experiment and analysis shows that the metric NRD correctly reflects the effort of different survivability strategy, and the proposed test method STNSD has good scalability and can be used to test and evaluate quantitative survivability in large scale network.

Thickness Dependence of Ultraviolet-excited Photoluminescence Efficiency of Lumogen Film Coated on Charge-coupled Device

  • Tao, Chunxian;Ruan, Jun;Shu, Shunpeng;Lu, Zhongrong;Hong, Ruijin;Zhang, Dawei;Han, Zhaoxia
    • Current Optics and Photonics
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    • v.1 no.4
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    • pp.284-288
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    • 2017
  • In order to investigate the ultraviolet-excited photoluminescence properties of phosphor coatings and their relationship to thickness, Lumogen coatings with different thicknesses were deposited on quartz substrates and charge-coupled device chips by thermal evaporation. The variation of the film thickness affected the crystallite size, surface roughness and fluorescence signal. It was found that the Lumogen coating with the thickness of 420 nm has the largest luminescent signal and conversion efficiency, and the corresponding coated charge-coupled devices had the maximum quantum efficiency in the ultraviolet. These results provided one key parameter for improving the sensitivity of Lumogen coated charge-coupled devices to ultraviolet light.

A Dual Selection Marker Transformation System Using Agrobacterium tumefaciens for the Industrial Aspergillus oryzae 3.042

  • Sun, Yunlong;Niu, Yali;He, Bin;Ma, Long;Li, Ganghua;Tran, Van-Tuan;Zeng, Bin;Hu, Zhihong
    • Journal of Microbiology and Biotechnology
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    • v.29 no.2
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    • pp.230-234
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    • 2019
  • Currently, the genetic modification of Aspergillus oryzae is mainly dependent on protoplast-mediated transformation (PMT). In this study, we established a dual selection marker system in an industrial A. oryzae 3.042 strain by using Agrobacterium tumefaciens-mediated transformation (ATMT). We first constructed a uridine/uracil auxotrophic A. oryzae 3.042 strain and a pyrithiamine (PT)-resistance binary vector. Then, we established the ATMT system by using uridine/uracil auxotrophy and PT-resistance genes as selection markers. Finally, a dual selection marker ATMT system was developed. This study demonstrates a useful dual selection marker transformation system for genetic manipulations of A. oryzae 3.042.

Performance Analysis of Key Exchange Protocols on ETSI Standard (ETSI 표준 키 교환 프로토콜의 성능 분석)

  • Lee, Young-Seok;Choi, Hoon
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.8 no.6
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    • pp.520-527
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    • 2015
  • The key exchange protocols are very crucial tools to provide the secure communication in the broadband satellite access network. They should be required to satisfy various requirements such as security, key confirmation, and key freshness. In this paper, we present the security functions in ETSI(European Telecommunications Standards Institute), and analyze the specification of the security primitives and the key exchange protocols for the authenticated key agreement between RCST(Return Channel Satellite Terminal) and NCC(Network Control Centre). ETSI key exchange protocols consists of Main Key Exchange, Quick Key Exchange, and Explicit Key Exchange. We analyse the pros and cons of key exchange protocols based on performance analysis and performance evaluation.