• Molecules and Cells
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• 제38권5호
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• pp.441-451
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• 2015

Recognition of cytosolic DNA initiates a series of innate immune responses by inducing IFN-I production and subsequent triggering JAK1-STAT1 signaling which plays critical roles in the pathogenesis of infection, inflammation and autoimmune diseases through promoting B cell activation and antibody responses. The stimulator of interferon genes protein (STING) has been demonstrated to be a critical hub of type I IFN induction in cytosolic DNA-sensing pathways. However, it still remains unknown whether cytosolic DNA can directly activate the JAK1-STAT1 signaling or not. And the role of STING is also unclear in this response. In the present study, we found that dsDNA directly triggered the JAK1-STAT1 signaling by inducing phosphorylation of the Lyn kinase. Moreover, this response is not dependent on type I IFN receptors. Interestingly, STING could inhibit dsDNA-triggered activation of JAK1-STAT1 signaling by inducing SHP-1 and SHP-2 phosphorylation. In addition, compared with normal B cells, the expression of STING was significantly lower and the phosphorylation level of JAK1 was significantly higher in B cells from MRL/lpr lupus-prone mice, highlighting the close association between STING low-expression and JAK1-STAT1 signaling activation in B cells in autoimmune diseases. Our data provide a molecular insight into the novel role of STING in dsDNA-mediated inflammatory disorders.

• 한국도로학회논문집
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• 제16권3호
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• pp.9-20
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• 2014

PURPOSES : This study is to compare load transfer efficiency of key joint and dowel joint for airport concrete pavement. METHODS : As AC150/5320-6D of FAA's [Advisory Circular] was changed into AC150/5320-6E, Key joint type of rigid pavement were excluded from Construction Joints. LTE(Load Transfer Efficiency) of dowel joint and key joint were compared by times and seasons through pavement temperature measurement, ocular investigation and HWD measurement. RESULTS : For the joint performance grade of No. 2(The second) runway of airport, 12% of poor rate was observed in key joint and 2% of poor rate in dowel joint. Poor rate of key joint was increased to 17%, if only No. 3~No. 6 slabs, which are mostly loaded from the airplanes, were applied for the study. In apron area, LTE poor rate of key joint was high in winter, and LTE poor rate of dowel joint was at least above 'Fair' grade. In summer, 'Fair' for key joint, 'Acceptable' for dowel joint appeared. CONCLUSIONS : As results, dowel joint was superior than key joint for LTE. Deviations of seasons and times were smaller in dowel joint's result. And LTE in winter was lower than LTE in summer.

• BMB Reports
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• 제40권2호
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• pp.270-276
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• 2007

We have cloned a novel mouse zinc finger protein gene Znf313 by rapid amplification of cDNA ends (RACE) according to the homologue of human ZNF313 gene. The cDNA is 2,163 base pairs (bp) in length and encodes a 229 amino acids (aa) protein with a $C_3HC_4$ ring finger domain and three $C_2H_2$ domains. 89% and 93% nucleotide (nt) and aa sequence identity is observed with its human homologue. Revealed by Northern blot and RT-PCR, full mRNA consists of 2.16 kb and widely expresses in tissues as a single transcript, most abundantly in heart, liver, kidney and testis. The expression of Znf313 in testis is detected in all development stages. Western blot analysis also reveals that Znf313 is expressed in the tissues. Immunohistochemical staining and subcellular localization demonstrate that Znf313 is expressed both in the cytoplasm and nucleus whereas predominantly localized in the nucleus. Present data suggests that Znf313 gene might play a fundamental role in gene transcription and regulation in organism and relates to spermatogenesis.

• Asian Pacific Journal of Cancer Prevention
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• 제16권15호
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• pp.6627-6632
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• 2015

Background: We conducted a study exploring the clinical safety and efficacy of decitabine in patients with acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS), combined with a complex karyotype. Materials and Methods: From April 2009 to September 2013, a total of 35 patients with AML/MDS combined with a complex karyotype diagnosed in the First Affiliated Hospital of Soochow University were included for retrospective analysis. All patients were treated with decitabine alone ($20mg/m^2$ daily for 5 days) or combination AAG chemotherapy (Acla 20mg qod*4d, Ara-C $10mg/m^2$ q12h*7d, G-CSF $300{\mu}g$ qd, the dose of G-CSF adjusted to the amount in blood routinely). Results: In 35 patients, 15 exhibited a complete response (CR), and 6 a partial response (PR), the overall response rate (CR+PR) being 60% (21 of 35). Median disease-free survival was 18 months and overall survival was 14 months. In the 15 MDS patients with a complex karyotype, the CR rate was 53.3% (8 of 15); in 20 AML patients with complex karyotype, the overall response rate was 65% (13 of 20). The response rate of decitabine alone (22 cases) was 56.5% (13 of 22), while in the combination chemotherapy group (13 cases), the effective rate was 61.5% (8 of 13)(P>0.05). There are 15 patients with chromosome 7 aberration, after treatment with decitabine, 7 CR, 3 PR, overall response rate was 66.7% (10 of 15). Of 18 patients with 3 to 5 kinds of chromosomal abnormalities, 66.7% demonstrated a response; of 17 with more than 5 chromosomal abnormalities, 52.9% had a response. In the total of 35 patients, with one course (23 patients) and ${\geq}$two courses (12 patients), the overall response rate was 40.9% and 92.3% (P<0.05). Grade III to IV hematological toxicity was observed in 27 cases (75%). Grade III to IV infections were clinically documented in 7 (20%). Grades I to II non-hematological toxicity were infections (18 patients), haematuria (2 patients), and bleeding (3 patients). With follow-up until September 2013, 7 patients were surviving, 18 had died and 10 were lost to follow-up. In the 6 cases who underwent allogeneic hematopoietic stem cell transplantation (HSCT) all were still relapse-free survivors. Conclusions: Decitabine alone or combination with AAG can improve outcome of AML/MDS with a complex karyotype, there being no significant difference decitabine in inducing remission rates in patients with different karyotype. Increasing the number of courses can improve efficiency. This approach with fewer treatment side effects in patients with a better tolerance should be employed in order to create an improved subsequent chance for HSCT.

• Asian Pacific Journal of Cancer Prevention
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• 제14권6호
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• pp.3575-3579
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• 2013

Background: The 2518 A/G polymorphism in the MCP-1 gene has been extensively studied for association swith cancer; however, results from replication studies have been inconsistent. The aim of this investigation was to determine links with risk of cancer by meta-analysis. Methods: We searched Pubmed, Embase, CNKI, Weipu and Wanfang databases, covering all case-control studies until March, 2013. Statistical analyses were performed using the Revman 5.0 software. Results: A total of 11 case-control studies met our inclusion criteria, including 1,422 cases and 2,237 controls. The results indicated that the MCP-1 2518 gene polymorphism had no association with cancer risk overall (GG vs.GA+ AA: OR = 0.89, 95%CI = 0.61-1.28, P = 0.52). However, in the subgroup analysis by ethnicity, a decrease of cancer risk was found in Asian populations (GG vs.GA+ AA: OR = 0.79, 95%CI = 0.63-0.99, P = 0.04). Conclusion: This meta-analysis suggested that the 2518A/G polymorphism of MCP-1 gene is associated with risk of cancer among Asian, but not in Caucasian populations.

• BMB Reports
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• 제47권2호
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• pp.86-91
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• 2014

Tissue-specific gene expression is regulated by epigenetic modification involving trans-acting factors. Here, we identified that the human MAGEB16 gene and its mouse homolog, Mageb16, are only expressed in the testis. To investigate the mechanism governing their expression, the promoter methylation status of these genes was examined in different samples. Two CpG islands (CGIs) in the 5' upstream region of MAGEB16 were highly demethylated in human testes, whereas they were methylated in cells without MAGEB16 expression. Similarly, the CGI in Mageb16 was hypomethylated in mouse testes but hypermethylated in other tissues and cells without Mageb16 expression. Additionally, the expression of these genes could be activated by treatment with the demethylation agent 5'-aza-2'-deoxycytidine (5'-aza-CdR). Luciferase assays revealed that both gene promoter activities were inhibited by methylation of the CGI regions. Therefore, we propose that the testis-specific expression of MAGEB16 and Mageb16 is regulated by the methylation status of their promoter regions.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제7권1호
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• pp.149-165
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• 2013

Current group key agreement protocols, which are often tree-based, have unnecessary delays that are caused when members with low-performance computer systems join a group key computation process. These delays are caused by the computations necessary to balance a key tree after membership changes. An alternate approach to group key generation that reduces delays is the dynamic prioritizing mechanism of queue-based group key generation. We propose an efficient group key agreement protocol and present the results of performance evaluation tests of this protocol. The queue-based approach that we propose is scalable and requires less computational overhead than conventional tree-based protocols.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제7권7호
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• pp.1737-1753
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• 2013

Current group key agreement protocols, which are often tree-based, have unnecessary delays that are caused when members with low-performance computer systems join a group key computation process. These delays are caused by the computations necessary to balance a key tree after membership changes. An alternate approach to group key generation that reduces delays is the dynamic prioritizing mechanism of queue-based group key generation. We propose an efficient group key agreement protocol and present the results of performance evaluation tests of this protocol. The queue-based approach that we propose is scalable and requires less computational overhead than conventional tree-based protocols.

• Archives of Pharmacal Research
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• 제20권3호
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• pp.288-290
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• 1997

An efficient synthesis of 3-(E)-hydroxy- and 3-(E)-acetoxypropenylcephem derivatives, key intermediates for the synthesis of 3-(E)-propenylcephalosporins was achieved via Stille coupling reaction of 3-trifloxycephem with 3-(E)-tributylstannylallylic alcohol.

• Journal of the Optical Society of Korea
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• 제19권4호
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• pp.351-356
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• 2015

Visible light communication (VLC) is considered to be an attractive scheme to realize the broadband wireless communication for an indoor environment. We present a room division multiplexing (RDM) mechanism for an indoor multi-room VLC network, which utilizes the spatial position of the LED lamp in different rooms as a novel dimension of network resource for multiplexing, and thus the network capacity is increased. In such a network, the service interruption caused by user cross-room movement is an important problem, and we propose a double-area-positioning based cross-room switch solution. An experimental platform demonstrates the RDM-deployed VLC network, and validates the performance of the presented switch mechanism.