• Journal of the Korean Society of Environmental Restoration Technology
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• v.15 no.2
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• pp.57-75
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• 2012

This study was carried out to investigate the flora of estuary regions, Namdaecheon, Jusucheon, and Maeupcheon, placed in the east coast of Gangwon-do. The vascular plants were summarized as 441taxa, including 90 families, 277 genera, 388 species, 2 subspecies, 35 varieties, and 6 forms. Number of species in each investigated region was 271taxa of Namdaecheon, 247taxa of Jusucheon, and 285taxa of Maeupcheon, respectively. Among the investigated 441taxa, 7 Korean endemic, 5 rare plants and 37 specially designated plants by Ministry of Environment were also included. Aquatic and hydrophyte plants were collected 41taxa, and the thinophyte and halophyte plants were investigated 22taxa. The naturalized plants were 59taxa, and the percent of naturalized plant species was estimated as 13.3%. Based on the floristic composition, Maeupcheon was estimated as well conserved estuary region among the investigated 3 rivers.

• Journal of The Korean Society of Grassland and Forage Science
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• v.38 no.1
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• pp.53-60
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• 2018

Yield prediction model for mixed pasture was developed with a shortage that the relationship between dry matter yield (DMY) and days of summer depression (DSD) was not properly reflected in the model in the previous research. Therefore, this study was designed to eliminate the data of the regions with distinctly different climatic conditions and then investigate their relationships DMY and DSD using the data in each region separately of regions with distinct climatic characteristics and classify the data based on regions for further analysis based on the previous mixed pasture prediction model. The data set used in the research kept 582 data points from 11 regions and 41 mixed pasture types. The relationship between DMY and DSD in each region were analyzed through scatter plot, correlation analysis and multiple regression analysis in each region separately. In the statistical analysis, DMY was taken as the response variable and 5 climatic variables including DSD were taken as explanatory variables. The results of scatter plot showed that negative correlations between DMY and DSD were observed in 7 out of 9 regions. Therefore, it was confirmed that analyzing the relationship between DMY and DSD based on each region is necessary and 5 regions were selected (Hwaseong, Suwon, Daejeon, Siheung and Gwangju) since the data size in these regions is large enough to perform the further statistical analysis based on large sample approximation theory. Correlation analysis showed that negative correlations were found between DMY and DSD in 3 (Hwaseong, Suwon and Siheung) out of the 5 regions, meanwhile the negative relationship in Hwaseong was confirmed through multiple regression analysis. Therefore, it was concluded that the interpretability of the yield prediction model for mixed pasture could be improved based on constructing the models using the data from each region separately instead of using the pooled data from different regions.

• Mycobiology
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• v.43 no.2
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• pp.166-169
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• 2015

We report the isolation of a Gongronella butleri species and describe it based on the analysis of the internal transcribed spacer region of rDNA and morphological characteristics. G. butleri has been reported as a high chitosan producer in the literature. This is the first record of G. butleri isolated from crop field soil in Korea.

• The Korean Journal of Mycology
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• v.45 no.3
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• pp.167-174
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• 2017

In the ongoing survey of fungal diversity in Korea, two Ascomycetes species, namely Rhinocladiella similis and Toxicocladosporium irritans, were isolated in 2016. These species were identified based on the internal transcribed spacer region and morphological characteristics. This is the first report of these species in Korea, and the morphological characteristics and images of the fungi are presented.

• The Korean Journal of Mycology
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• v.45 no.2
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• pp.107-113
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• 2017

Aphanoascus fulvescens KNU16-343 was isolated from crop field soil and identified based on morphological and molecular characteristics. The shape and size of conidia and conidiophores, as well as the internal transcribed spacer region of rDNA, confirmed that the isolate was A. fulvescens. This is the first report of this fungal species in Korea.

• The Korean Journal of Mycology
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• v.44 no.3
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• pp.145-149
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• 2016

Pseudeurotium bakeri KNU14-21 isolated from crop field soil is a previously unreported fungal species in Korea. This fungal species was identified through morphological and molecular characteristics. Based on the shape and size of conidia and conidiophores as well as the internal transcribed spacer region of rDNA, the isolate was confirmed to be Pseudeurotium bakeri. This species has not been previously reported from Korea, and this is the first report of its incidence in Korea.

• Mycobiology
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• v.46 no.4
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• pp.297-304
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• 2018

Two new records of Trichocomaceae, namely Aspergillus allahabadii and Penicillium sizovae, were isolated in 2016 during a survey of fungal diversity in different crop fields locations in Gyeongnam, Korea. These species were identified based on morphological characters and phylogenetic analysis using internal transcribed spacer region and ${\beta}-tubulin$-encoding gene sequence data. A. allahabadii and P. sizovae have not yet been reported in Korea. Thus, this is the first report of these species in Korea, and their descriptions as well as details of their morphological characters are presented.

• Molecules and Cells
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• v.25 no.1
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• pp.112-118
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• 2008

Laccases are multicopper-containing oxidases that catalyze the oxidation of many aromatic compounds with concomitant reduction of oxygen to water. Interest in this enzyme has arisen in many fields of industry, including detoxification, wine stabilization, paper processing, and enzymatic conversion of chemical intermediates. In this study, we cloned a laccase gene (GLlac1) from the white-rot fungus Ganoderma lucidum. The cloned gene consists of 4,357 bp, with its coding region interrupted by nine introns, and the upstream region has putative CAAT and TATA boxes as well as several metal responsive elements (MREs). We also cloned a full-length cDNA of GLlac1, which contains an uninterrupted open reading frame (ORF) of 1,560 bp coding for 520 amino acids with a putative 21-residue signal sequence. The DNA and deduced amino acid sequences of GLlac1 were similar but not identical to those of other fungal laccases. GLlac1 was released from the cells when expressed in P. pastoris, and had high laccase activity. In addition, GLlac1 conferred antioxidative protection from protein degradation, and thus may be useful in bio-medical applications.

• Journal of Microbiology
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• v.43 no.1
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• pp.44-48
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• 2005

In our previous study, the first structural gene (GGTI) encoding ${\gamma}-glutamyl$ transpeptidase was cloned and characterized from the fission yeast Schizosaccharomyces pombe, and its transcription, using the GGTI-lacZ fusion gene, containing the 1,085 bp upstream region from the translational initiation point, was found to be enhanced by sodium nitroprusside and L-buthionine-(S,R)-sulfoximine (BSO). In the present work, regulation of the GGTI gene was further elucidated. Non-fermentable carbon sources, such as acetate and ethanol, markedly enhanced the synthesis of ${beta}-galactosidase$ from the GGTI-lacZ fusion gene. However, its induction by non-fermentable carbon sources appeared to be independent of the presence of the Pap1 protein. Nitrogen starvation also gave rise to induction of GGTI gene expression in a Pap1-independent manner. The three additional fusion plasmids, carrying 754, 421 and 156 bp regions, were constructed. The sequence responsible for the induction by non-fermentable carbon sources and nitrogen starvation was identified to exist within a -421 bp region of the GGTI gene. Taken together, the S. pombe GGTI gene is regulated by non-fermentable carbon sources and nitrogen starvation.

• Horticulture, Environment, and Biotechnology : HEB
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• v.59 no.6
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• pp.865-873
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• 2018

Allium ochotense and Allium microdictyon are commonly known as 'Mountain garlic' and are popular, economically important species in many countries such as Korea, China, and Mongolia. Their leaves are used as culinary side dishes and in traditional medicines. In Korea, these two species are at risk of extinction due to damage to their natural habitat and thus, conservation and breeding programs are needed. However, their identification relies mostly on morphological data, which is limited and until recently, led to classifying these two species under A. victorialis. In the present study, a simple and reliable method of molecular identification was developed to distinguish A. ochotense from A. microdictyon that targets four barcoding regions: the internal transcribed spacer (ITS), the maturase K gene (matK), the chloroplast psbA-trnH intergenic region, and the ribulose-bisphosphate carboxylase large subunit gene (rbcL). Single nucleotide polymorphisms (SNPs) were found in ITS and matK regions, and species-specific primers were designed based solely on the SNP at position 680 of the ITS region that could differentiate A. ochotense from A. microdictyon. Using these primers in amplification refractory mutation system (ARMS)-PCR, A. ochotense, and A. microdictyon could be simultaneously and efficiently distinguished. This study is the first to report a simple, rapid, and efficient method for discriminating A. ochotense and A. microdictyon, indicating the utility of species-specific markers in the development of conservation and breeding programs.