• Korean Journal of Veterinary Research
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• v.39 no.3
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• pp.545-553
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• 1999

A 474-base pair segment covering the hypervariable region of VP2 gene from six Korean infectious bursal disease virus(K-IBDV) isolates(K1, K2, SH/92, 225, 269, 310) and one attenuated IBDV(DAE) were amplified using RT-PCR, sequenced, and compared with published sequences for IBDV. K-IBDV isolates(K1, K2, SH/92, 225, 269) and foreign very virulent(vv) IBDV strains had 94.93~100% amino acid sequence similarity. K-IBDV isolate 310 and other K-IBDV isolates had 84.31~86.07% amino acid sequence similarity. Attenuated strain(DAE), like other attenuated strain, has substitution at positions 279(D to N) and 284(A to T) as well as in the serine-rich heptapeptide region. Five K-IBDV isolates except 310 isolate share unique amino acid residues at positions 222(A), 256(I), 294(I) which are not present in other standard and attenuated strains. At the two hydrophilic region, K-IBDV isolates except 310 isolate had identical amino acids comparing with Belgium vv IBDV 894VB but had four amino acid substitutions comparing with Chinese vv IBDV F9502. The SWSASGS heptapeptide is conserved in all K-IBDV isolates. The sequence of K-IBDV isolate 310 was markedly different from other IBDV strains, evolving from a separate lineage than the others. By phylogenetic analysis, Five K-IBDV isolates except 310 isolate were categorized in one group with foreign vv IBDV isolates but K-IBDV isolate 310 was categorized in a separate group which was differentiated from other compared IBDV strains.

• Research in Plant Disease
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• v.17 no.2
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• pp.169-176
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• 2011

Phytophthora katsurae is the fungus responsible for chestnut ink disease. The objectives of this study were to determine if a single-strand conformation polymorphism (SSCP) analysis of rDNA-ITS region, elongation factor 1 alpha gene and ${\beta}$-tubulin gene could be used for rapid identification and genetic diversity of P. katsurae, and to assess the potential use of the SSCP technique as a diagnostic tool for P. katsurae. Each regions amplified by PCR using primers designed to overlap the genus Phytophthora were characterized for the Phytophthora species. PCR products were denatured and electrophoresed for SSCP analysis. P. katsurae isolates showed an unique pattern in SSCP analysis and were easily distinguished from other Phytophthora species used as the control. This indicates that SSCP analysis is an useful technique for distinguishing Phytophthora species from genetically close relatives, and show that the SSCP analysis of each region is an efficient detection tool for P. katsurae. But PCR-SSCP analysis of single-gene may have difficulty in distinguishing P. katsurae from other Phytophthora species. Therefore, PCR-SSCP analysis of multi-genes can be useful for rapid and effective identification of P. katsurae.

• Korean Business Review
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• v.22 no.2
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• pp.35-59
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• 2009

The drive for sustainable economic growth for the sub Saharan African region continues to occupy a central place in the debate of how to move the region forward. For decades, governments, policy makers, Non Governmental Organizations and world bodies including the World Bank, IMF, ADB, USIAD and the European Union have engaged policies aimed at bringing solutions to the horrendous poverty crisis to nations of this region. Despite these noble actions and intents, poverty and underdevelopment has prevailed in countries of the region such as Cameroon. Cameroon is mainly an agricultural economy with its products facing declining prices and competition from synthetic substitutes resulting to deficits of balance of trades. This has resulted to borrowing and debt. At the same time, it is a country blessed with an abundance of tourist resources. From the literature review, tourism potency to economic growth is overwhelming. This research was motivated by the quest to find answers to questions such as; why development policies during the last two decades not succeeded in achieving Economic growth in countries of this region particularly Cameroon and why the country/ region still beleaguered by poverty and debt despite haven implemented various economic development plans. In recent years, the role of tourism has become increasingly recognized in its role of economic growth and poverty alleviation. This study attempts to unveil tourism's contribution to economic growth and to push for Tourism development as an al ternative economic growth alternative to Cameroon. Previous economic policies have ignored to tie economic growth within the country's socio-economic, geo-political and environmental circumstances. Findings from this work suggest that any sound economic policy can not afford to ignore the country's stock of both human and fiscal capital. Findings presented herewith validate Tourism as a feasible indigenous economic growth alternative that helps bringing employment, capital investment and protect the environmental ruin.

• Journal of Korean Society of Forest Science
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• v.85 no.1
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• pp.56-65
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• 1996

Current volume tables might underestimate or overestimate the volumes of individual trees in a specific region because the tables were made using the data from broad region. This study provides a statistical method of local correction, which is the ratio-of-means estimator, when the table is applied to the data from a specific region. Data used in this study were 411 trees of Larix leptolepis from Hongchon region. Five statistical models for individual tree volume equation were evaluated based on 3 evalation criteria and the best equation fitted to the data from Hongchon region was selected. The volume estimated by the selected equation was then compared with the volume estimated by the current volume table. From the ratio-of-means estimate based on the volumes estimated by selected equation and by current volume table, the local correction was made. The correction equation was $V_{Hongchon}=1.078$ $V_{volume\;table}$. It is also proved that the correction equation can simply and precisely estimate tree volumes of Larix leptolepis in Hongchon region using the current volume table.

• BMB Reports
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• v.36 no.3
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• pp.326-331
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• 2003

The fission yeast cells that contained the cloned glutathione synthetase (GS) gene showed 1.4-fold higher glutathione (GSB) content and 1.9-fold higher GS activity than the cells without the cloned GS gene. Interestingly, $\gamma$-glutamylcysteine synthetase activity increased 2.1-fold in the S. pombe cells that contained the cloned GS gene. The S. pombe cells that harbored the multi copy-number plasmid pRGS49 (containing the cloned GS gene) showed a higher level of survival on solid media with cadmium chloride (1 mM) or mercuric chloride ($10\;{\mu}M$) than the cells that harbored the YEp357R vector. The 506 bp upstream sequence from the translational initiation point and N-terminal8 amino acid-coding region were fused into the promoteriess $\beta$-galactosidase gene of the shuttle vector YEp367R to generate the fusion plasmid pUGS39. Synthesis of $\beta$-galactosidase from the fusion plasmid pUGS39 was significantly enhanced by cadmium chloride and NO-generating S-nitroso-N-acetylpenicillamine (SNAP) and sodium nitroprusside (SN). It was also induced by L-buthionine-(S,R)-sulfoximine, a specific inhibitor of $\gamma$-glutamylcysteine synthetase (GCS). We also found that the expression of the S. pombe GS gene is regulated by the Atf1-Spc1-Wis1 signal pathway.

• Korean Journal of Ecology and Environment
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• v.45 no.1
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• pp.62-71
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• 2012

Lake Doam watershed was surveyed to evaluate non-point source discharge characteristics and discharge load including several water quality parameters in Song Stream from July 2009 to July 2011. Concentrations of water pollutants were high during the rainfall period, especially, SS, TP and COD showed increasing tendencies toward cumulative water discharge but TN did not show much difference. SS, TP and COD had an initial flush effect of over 50 mm rainfall event but there was no clear tendency for rainfalls below that level. Event mean concentration (EMC) regarding the rainy and dry period showed large differences. Especially rainy season EMC (SS, TP, COD) demonstrated an increasingly high tendency. EMCs of COD, SS, TN and TP measured for twelve rain events were as high as 26.1, 866.0, 4.68 and 0.605 mg $L^{-1}$, respectively. COD, SS, TN and TP loadings from the highland agricultural region of the Song Stream watershed were 34,263, 1,250,254, 2,673 and 933 kg $yr^{-1}\;km^{-2}$, respectively, which were relatively higher than the results of other stream systems. Therefore, it is strongly recommended that long-term monitoring and non-point pollution reduction programs for the highland agricultural area to continue. Furthermore, this non-point source pollution loading research acquired from the highland agricultural area could be the base for reassessment.

• Journal of Forest and Environmental Science
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• v.34 no.4
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• pp.304-312
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• 2018

This study investigates to identify the impact factors of timber production cost on the relationship between production cost components and revenues generated by evaluating the entire timber supply chain. In this research, selected 13 logging zones as target areas and classified 14 forest production cost factors, six groups. Additionally, established 13 externalenvironmental factors with related stakeholders and forestry experts. And the BCR (Benefit Cost Ratio) method was then used to analyze the economics of each zone. Filled up a checklist and rated using 5 point scale for each target region, and extracted major cost factors for the production economy of the item. The analysis of major cost factors in the timber production revealed that wood grab equipment usage fee was the first ranked and forest trees purchase cost was ranked in the $2^{nd}$Also, the $3^{rd}$ranking was logging expenses, and transport cost, which accounted for 84% of the total cost, was ranked in the $4^{th}$. In addition, the rock land ratio, slope, timber payment (forest trees purchase cost), special timber, ratio of timber, DBH (Diameter at Breast Height), and mixed forest ratio were the factors that most affected the timber supply chain cost.

• Molecules and Cells
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• v.27 no.4
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• pp.459-465
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• 2009

MUG1 is a MULE transposon-related domesticated gene in plants. We assessed the sequence diversity, neutrality, expression, and phylogenetics of the MUG1 gene among Oryza ssp. We found MUG1 expression in all tissues analyzed, with different levels in O. sativa. There were 408 variation sites in the 3886 bp of MUG1 locus. The nucleotide diversity of the MUG1 was higher than functionally known genes in rice. The nucleotide diversity (${\pi}$) in the domains was lower than the average nucleotide diversity in whole coding region. The ${\pi}$ values in nonsynonymous sites were lower than those of synonymous sites. Tajima D and Fu and Li $D^*$ values were mostly negative values, suggesting purifying selection in MUG1 sequences of Oryza ssp. Genome-specific variation and phylogenetic analyses show a general grouping of MUG1 sequences congruent with Oryza ssp. biogeography; however, our MUG1 phylogenetic results, in combination with separate B and D genome studies, might suggest an early divergence of the Oryza ssp. by continental drift of Gondwanaland. O. long-istaminata MUG1 divergence from other AA diploids suggests that it might not be a direct ancestor of the African rice species.

• Journal of Veterinary Clinics
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• v.40 no.1
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• pp.8-15
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• 2023

Atopic dermatitis (AD) is a common skin disease in animals and several therapeutic trials with various drugs have been conducted for more effective management of AD. However, these trials have not been able to properly address all the aspects of AD management because of the lack of good efficacy or due to significant side effects of the drugs being tested. Synbio-glucan functional spray is a functional skin spray using Synbio-glucan composed of β-glucan and probiotics. We designed a functional spray composed of Synbio-glucan (patent application number:10-1805863), distilled water, glycerin, solubilizer, and 40% alcohol. We tested the efficacy and safety of the functional spray on six dogs with AD. The trial was conducted with the consent of the caregivers. The spray was applied to the skin lesions, including the trunk, axillae, inguinal region, or periocular areas, thrice a day for 30 days. To evaluate the efficacy of this functional spray, we assessed the pruritus visual analog scale (PVAS) and the canine atopic dermatitis extent and severity index (CADESI)-4. At the end of one month, the results clinical scores after functional spray treatment showed a significant decrease in the PVAS (p = 0.03) and CADESI-4 (p = 0.03) in all the subject dogs with AD. This study thus confirmed that the Synbio-glucan functional spray is efficacious and safe for the treatment of AD in dogs.

• Journal of The Korean Society of Grassland and Forage Science
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• v.43 no.3
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• pp.190-198
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• 2023

This study was conducted to calculate the damage of Italian ryegrass (IRG) by abnormal climate using machine learning and present the damage through the map. The IRG data collected 1,384. The climate data was collected from the Korea Meteorological Administration Meteorological data open portal.The machine learning model called xDeepFM was used to detect IRG damage. The damage was calculated using climate data from the Automated Synoptic Observing System (95 sites) by machine learning. The calculation of damage was the difference between the Dry matter yield (DMY)_normal and DMY_abnormal. The normal climate was set as the 40-year of climate data according to the year of IRG data (1986~2020). The level of abnormal climate was set as a multiple of the standard deviation applying the World Meteorological Organization (WMO) standard. The DMY_normal was ranged from 5,678 to 15,188 kg/ha. The damage of IRG differed according to region and level of abnormal climate with abnormal temperature, precipitation, and wind speed from -1,380 to 1,176, -3 to 2,465, and -830 to 962 kg/ha, respectively. The maximum damage was 1,176 kg/ha when the abnormal temperature was -2 level (+1.04℃), 2,465 kg/ha when the abnormal precipitation was all level and 962 kg/ha when the abnormal wind speed was -2 level (+1.60 ㎧). The damage calculated through the WMO method was presented as an map using QGIS. There was some blank area because there was no climate data. In order to calculate the damage of blank area, it would be possible to use the automatic weather system (AWS), which provides data from more sites than the automated synoptic observing system (ASOS).