• Journal of Environmental Science International
• /
• v.25 no.5
• /
• pp.655-671
• /
• 2016

The distribution characteristics of functional feeding groups and biological evaluation of water quality with benthic macroinvertebrates were investigated from 2013 to 2014 in the mountain streams of Jirisan National Park. Both quantitative and qualitative collection had been performed six times during study periods in twelve sites located at upstream, midstream and downstream of four valleys, Chilsungol, Baemsagol, Daesunggol and Piagol of freshwater ecosystem in Jirisan National Park. As results, the total collected benthic macroinvertebrates were 3 phyla, 4 classes, 11 orders, 37 families, 107 species and 4,028 individuals. In 2013, 3 phyla, 4 classes, 10 orders, 32 families, 89 species and 1,829 individuals were collected. And in 2014, 3 phyla, 4 classes, 9 orders, 31 families, 80 species and 2,199 individuals were identified. The total dominant species was Ecdyonurus kibumensis, and EPT groups occupied 80.39% of total species number. Biotic indices were also compared to site and to season. The dominant index (DI) was highest at the downstream of Baemsagol in the spring of 2013, and the evenness index (J') was highest at the downstream of Daesunggol in the summer of 2013. Both diversity index (H') and richness index (RI) were highest at the upstream of Baemsagol in the summer of 2014. The correlation between altitude above sea level and functional feeding groups of benthic macroinvertebrates was also researched in this study. As a result, the species number of shredders and predators have significantly increased according to the increase of the altitude of their habitat. On the other hand, individuals of gathering collectors have increased according to the increase of altitude. The biological water quality was evaluated and compared with several methods in this study, and we suggest that ESB and GPI are more appropriate than KSI and BMI to evaluate biological water quality of mountain stream water.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2012.05a
• /
• pp.728-728
• /
• 2012

본 연구에서는 자연하상 복원을 위한 하상복원공법을 개발하여 시험구간에 적용하였다. 공법적용 대상 지역은 안양시역내의 안양천 일정구간으로 화창교로부터 상류방향으로 약 500m 거리에 웅덩이 수제공법이 적용되어 있으며, 그로부터 다시 상류방향으로 130m 떨어진 거리에 낮은 바닥막이 공법이 적용되어 있다. 낮은 바닥막이 공법의 경우 완만한 곡류사주의 직상류 부로서 강한 수류와 함께 빠른 유속이 형성될 경우 하상의 세굴이 예상되어 이를 방지하기 위해 저수로의 유하방향으로 완만한 호형으로 설치하였으며, 웅덩이형수제 공법은 완만한 곡류로 좌안 수충부가 형성되어 있어 물의 흐름방향과 유속을 제어하여 흐름에 의한 호안침식을 방지하기 위해 현 위치를 선정 및 적용하였다. 첫 번째, 경관모니터링 결과 낮은 바닥막이 공법과 웅덩이 수제 공법을 적용한 구간에서 대규모 홍수 이후에도 저수로 호안과 하상의 안정성이 유지되었으며, 이는 공법 설치 전 후 하상 경관의 변화가 거의 없어 수리적 안정성 유지를 하고 있다고 볼 수 있다. 또한 웅덩이 수제 공법 설치 후, 미소서식처가 형성되면서 포식자인 왜가리, 백로류를 지속적으로 관찰할 수 있어 어류서식처로서의 기능이 강화되었음을 간접적으로 확인하였다. 두 번째, 생태계 모니터링 결과 저서성 무척추동물의 경우 한국오수생물지수(KSI)는 낮은 바닥막이 공법을 적용하여 하상구조가 변화된 조사 지점 st.1의 환경상태가 "양호"에서 "최적"으로 변화하였기 때문에 공법 적용이 서식처 건강성 증진에 도움을 준 것으로 판단된다. 세 번째, 수질 모니터링 결과 용존산소의 경우 상부지점보다 하부지점이 낮게 나타나는 결과를 보여주었다. 낮은 바닥막이 공법에 의한 용존산소 상승효과보다는 다양한 서식처의 제공이라는 측면이 보다 중요한 효과를 나타낸다. 결과적으로, 공법의 실용화를 위한 적용 공법에 대한 생태계 모니터링 및 평가를 통하여 하천에서의 다양한 서식처(Habitat) 창출을 통해 수생 생물의 다양성을 증진시키고, 하천 하상공법에 대한 생태계의 조사 및 평가기법을 확보하여 연구성과를 다른 하천으로의 확대 적용 할 수 있을 것으로 기대한다.

• Korean Journal of Ecology and Environment
• /
• v.45 no.1
• /
• pp.72-81
• /
• 2012

We investigated the effects of acid mine drainage (AMD) from abandoned coal mines on benthic macroinvertebrate communities in the upper reaches of the Nakdong River from May to October in 2009. Qualitative and quantitative sampling (Surber sampler: $50{\times}50$ cm; mesh size, 0.2 mm) was conducted at 7 study sites and 3 control sites in the study area. We thus sampled 117 species belonging to 53 families, 15 orders, 6 classes, and 5 phyla; the Ephemeroptera, Plecoptera, and Trichoptera group (EPT-group) represented the majority of the benthic macroinvertebrate community (71 species; 64.5%). In the quantitative sampling, a total of 11,575 individuals belonging to 58 species of benthic maroinvertebrates were sampled from the study sites (Sites 1-7), whereas 2,844 individuals belonging to 79 species were sampled from the control sites (Sites A-C). Tolerant species such as oligochaetes, $Epeorus$ $pellucidus$, $Baetis$ $fuscatus$, Hydropsychidae species, and Chironomidae species were predominant in the study sites. The community indices for the study sites, such as MacNaughton's dominance index (DI) (mean${\pm}$SD, $0.52{\pm}0.21$; range, 0.33-0.85) and the Shannon diversity index ($H^{\prime}$) ($2.06{\pm}0.60$; 1.06-2.57), were different from those for the control sites (DI: $0.29{\pm}0.07$, 0.22-0.35; $H^{\prime}$: $3.13{\pm}0.14$; 3.03-3.30). In the study sites, shredders and scrapers were scarce, whereas gathering-collectors (mainly Chironomidae species) were relatively abundant, as were clingers and burrowers. The detrended correspondence analysis (DCA) and similarity analyses showed that benthic macroinvertebrate communities in the study sites were clearly separated from those of the control sites, with the greatest dissimilarity being noted at the uppermost study site (Site 1), which is located close to an abandoned coal mine. The Korean saprobic index (KSI) and the ecological score using benthic macroinvertebrates (ESB) showed that the uppermost study site (Site 1) was ${\alpha}$-mesosaprobic or heavily polluted, whereas other study sites were in a fair or relatively good condition.

• Journal of Microbiology and Biotechnology
• /
• v.10 no.3
• /
• pp.349-354
• /
• 2000

Abstract A facultative alkalophilic gram-negative Vibrio metschnikovii strain RH530, isolated from the wastewater, produced several alkaline proteases (VAP) including six alkaline serine proteases and a metalloprotease. From this strain, high yielding YAP mutants were isolated by NTG treatment. The isolated mutant KS1 showed nine times more activity than the wild-type after optimization of the culture media. The production was regulated by catabolite repression when glucose was added to the medium. The effects of several organic nitrogen sources on the production of the YAP were investigated to avoid catabolite repression. The combination of 4% wheat gluten meal (WGM), 1.5% cotton seed flour (eSF), and 5% soybean meal (SBM) resulted in the best production when supplemented with 1% NaCl. The YAP showed a resistance to surfactants such as $sodium-{\alpha}-olefin$ sulfonate (AOS), polyoxy ethylene oxide (POE), and sodium dodecyl sulfate (SDS), yet not to linear alkylbenzene sulfonate (LAS). However, the activity of the YAP was restored completely when incubated with LAS in the presence of POE or $Na_2SO_4$. The YAP was stable in a liquid laundry detergent containing 6.6% SLES (sodium lauryl ether sulfate), 6.6% LAS, 19.8% POE, and stabilizing agents for more than two weeks at $40^{\circ}C$, but the stability was sharply decreased even after 1 day when incubated at $60^{\circ}C$. A washing performance test with the YAP exhibited it to be a good washing power by showing 51 % and 60% activity at $25^{\circ}C{\;}and{\;}40^{\circ}C$, respectively, thereby indicating that the YAP also has a good detergency at a low temperature. All the results suggest that the YAP produced from the mutant strain KSI has suitable properties for use in laundry detergents.rgents.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2009.05a
• /
• pp.496-500
• /
• 2009

소하천은 하천 네트워크의 최상류에 넓게 소재하는 하천이다. 본 연구의 목적은 올바른 소하천 수생태계 관리방안을 도출하기 위하여 소하천의 건강성을 분석하고 현행 소하천 관리제도의 개선점을 모색하는 것이었다. 본 연구에서는 다음과 같은 분석방법을 이용하였다. 첫째, 2007년 한강수계 소하천 28개 지점에서의 부착조류($DAI_{PO}$, TDI), 저서성 대형무척추동물(KSI), 어류(IBI), 서식환경, 수변환경 등 6개 항목에 대한 수생태 건강성 조사결과를 토대로 공간적 분포와 수질현황을 조사하여 소하천에 대한 종합적인 건강성 평가를 실시하였다. 둘째, 분석대상지역을 각각 소하천 구간스케일(28개 지점)과 유역스케일(팔당호, 안성천 유역)로 선정, 하천차수도(1:25,000)를 활용하여 해당 구간과 유역의 소하천도를 작성하였다. 셋째, 작성된 소하천도 는ArcGIS(ver. 9.3)에서 30, 60, 90, 120, 150-m Buffering을 하였다. 다음으로 소하천 구간은 중분류(23개 항목, $2000{\sim}2006$ 또는 2007년) 토지피복도를, 팔당호 및 안성천 유역의 소하천은 대분류(8개 항목, $1975{\sim}2000$년) 토지피복도를 적용하여 분류항목별 면적변화비율을 산정하였다. 끝으로 소하천 정비에 대한 제도적 문제점을 분석하여 소하천 관리의 개선점을 연구하였다. 그 연구 결과, 첫째 연구대상 소하천(28개)의 건강성은 도심 소하천에서 가장 낮게 조사되었고, 일부 소하천은 비록 상류에 위치함에도 불구하고 부착조류의 유기물, 영양염류 평가가 낮게 평가되었다. 둘째, 소하천 구간 스케일의 수변토지피복변화 분석결과 소하천 수생태 건강성은 거시적으로 산림, 도시화, 밭 등의 피복변화에 민감한 것으로 나타났으며 도시화 피복변화의 영향은 수변 30m에서 60m보다 3배 정도 큰 것으로 나타났다. 유역 스케일 분석에서는 상대적으로 도시화가 많이 진행된 안성천 유역의 소하천이 팔당호 유역보다 낮은 건강성일 것으로 예측되었다. 결론적으로 적절한 소하천 수변관리는 지역 하천의 건강성을 온전히 회복시키고 개선 유지하기 위한 중요한 수단들 중에 하나이며 수변토지피복의 변화율은 (소)하천 건강성 또는 유역관리의 지표로 활용될 수가 있는 것으로 조사되었다. 이와 더불어 본 연구를 통해 소하천 복원 및 관리는 소하천 특성을 고려한 장기적인 계획과 관리대상의 우선순위를 바탕으로 점진적인 대안마련이 필요할 것으로 사료된다.

• Korean Journal of Microbiology
• /
• v.39 no.1
• /
• pp.16-20
• /
• 2003

A phylogenetic analysis of bacterial populations inhabiting soil derived from serpentine was conducted. The samples were collected from adjacent metamorphic rocks and serpentinite soil at Kwangcheon. The pH of the serpentine areas ranged from 8.5 to 9.2. The number of bacteria on the DAL medium which was diluted with $10^{-2}$ of AL medium was 10~100 fold higher than that from the full strength of AL medium, and which indicates that oligotrophs are distributed in the serpentinite soil. Of a total of 76 isolates, 42 isolates were oligotrophic bacteria, which grew only on the DAL medium. Based on a phylogenetic analysis using 16S rDNA sequences, these isolates are found to fall within five major phylogenetic groups: proteobacteria $\alpha$-subdivision (3 strains), $\alpha$-subdivision (7 strains), $\gamma$-subdivision (2 trains); high G+C gram-positive bacteria (19 strains); low G+C grampositive bacteria (14 strains). Bacteria of the genus Streptomyces (high G+C division) and Bacillus (low G+C division) have been considered to form a numerically important fraction of serpentinite soil. Oligotrophic strains categorized as Afipia ($\alpha$-subdivision), Ralstonia, Variovorax ($\beta$-subdivision), Pseudomonas ($\gamma$ -subdivision), Arthrobacter (high G+C division), and Streptomyces (low G+C division).

• KSBB Journal
• /
• v.15 no.2
• /
• pp.188-194
• /
• 2000

The anaerobic bacterium P. productus was known to produce acetate from CO, C02 and H2. In this research the acetate f formation from waste gas was studied. For this research, kinetic parameter study on CO conversion were carried out. From t this study maximum CO conversion rate of 39.3 mmol/L . hr . 0.0 and Km of 0.578 atm were obtained. Also the effect of c CO refreshment, N source, initial pH and c비ture temperature on acetate formation were studied. Acetate formation in 5L lab s scale fermenter was tested and specific acetate production rate of 0.48 g/L-hr-O.O. was obtained and the acetate c concentration was 21 g/L.

• The Journal of The Korea Institute of Intelligent Transport Systems
• /
• v.19 no.6
• /
• pp.49-60
• /
• 2020

The number of traffic control equipment installed to prevent traffic accidents increases every year due to continuous installation by the National Police Agency and local governments. However, it is installed based on qualitative judgment rather than engineering analysis results. The purpose of this study was to present additional installations in the future by presenting the installation criteria considering the severity of accidents for each road type and calculating the appropriate number of installations. ARI indicators that can indicate the severity of traffic accidents were developed, and road types were classified through analysis of variance and cluster analysis, and accident information by road type was analyzed to derive ARI of clusters with high traffic accident severity. The ARI values required to determine the installation of equipment for each road type were presented, and 5,244 additional installation points were analyzed.