• Title/Summary/Keyword: KML-IIU

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Preliminary Toxicity and General Pharmacology of KML-IIU, a Purified pectin from Korean Mistletoe (Viscum album coloratum) (한국산 겨우살이 (Viscum album coloratum)로부터 정제된 렉틴 성분 KML-IIU의 예비 독성 및 일반 약리 시험)

  • 강태봉;윤택준;김종배;송성규;이관희
    • YAKHAK HOEJI
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    • v.45 no.3
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    • pp.251-257
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    • 2001
  • The study was carried out to evaluate the preliminary toxicity and general pharmacology of KML-IIU, a purified pectin from Korean Mistletoe (Viscum album coloratum). KML-IIU was administered intravenously to ICR mice and Spargue-Dawley rats to investigate the acute toxicity. LD50 values in mice and rats were above 30 $\mu$g/kg. KML-IIU had no effects on the general behaviors, acetic acid induced writhing syndrome, pentobarbial induced sleeping time, pentylenetetrazole induced convulsion and the change of body temperature. In addition, KML-IIU did not show any effects on digestive system and blood coagulation system.

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Isolation and Characterization of Two Korean Mistletoe Lectins

  • Kang, Tae-Bong;Song, Seong-Kyu;Yoon, Taek-Joon;Yoo, Yung-Choon;Lee, Kwan-Hee;Her, Erk;Kim, Jong-Bae
    • BMB Reports
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    • v.40 no.6
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    • pp.959-965
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    • 2007
  • Two isolectins (KML-IIU and the KML-IIL) were individually isolated from the previously reported Korean mistletoe lectin, KML-C, by using an immunoaffinity column. Molecular weights of the KML-IIU and the KML-IIL were 64 kDa and 60 kDa respectively. Both of the lectins were composed of heterogeneous A and B subunits linked with a disulfide bond, and showed the same carbohydrate-binding specificities for Gal and GalNAc. However, they are different not only in biophysical properties (glycosylation and amino acid compositions) but also bioactivities (cell killing and cytokine induction). The KML-IIL showed 17-145 times stronger in cytotoxicities to various human and mouse cancer cell lines than the KML-IIU. The KML-IIL also induced TNF-$\alpha$ secretion from mouse peritoneal macrophages 4.5 times better than the KML-IIU. The results demonstrated isolectins in Korean mistletoe were varied in bioactivities and the KML-IIL may be developed as an anti-cancer agent.

Identification of two lectins containing the same sugar-specificity from Korean mistletoe

  • Kang, Tae-Bong;Yoo, Yung-Choon;Yoon, Seok-Min;Lee, Kwan-Hee;Kim, Jong-Bae
    • Proceedings of the PSK Conference
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    • 2003.04a
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    • pp.213.2-214
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    • 2003
  • Three mistletoe lectins (ML -I, ML -IIU, ML -IIL) have been identified in Europe based on sugar specificities for galactose(Gal) and N-acetyl galactosamine(GalNAc). Korean mistletoe lectins have been known as mainly ML -II type. In previous results, we suggested that there are two lectins, 64 KDa and 60 KDa, in Korean mistletoe lectin (KML -C). (omitted)

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