• Korean Journal Plant Pathology
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• v.14 no.5
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• pp.371-375
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• 1998

Tobacco (Nicotiana tabacum) cultivars including NC 82 and KF 114, and Datura stramonium, Physalis floridana, Gomphrena globosa, and Chenopodium spp. were added to the previous host plants tested for the further examination on the biological characteristics of tobacco mosaic virus (TMV) strains isolated from tobacco (TMV-Common), tomato (TMV-Tomato), and pepper (TMV-Pepper), In TMV-Tomato and TMV-Pepper, different symptoms were noted in P. floridana (no symptom development), and NC 82 (local lesion production on the inoculated leaves) from TMV-Common with which systemic mosaic symptoms were developed. Local lesions were developed in KF 114, D. stramonium, G. globosa, and Chenopodium spp by TMV-Common and TVM-Tomato, while no symptom was observed in KF114 and G. globosa. Also the number and size of local lesions were smaller in KF 114 than Xanthi-nc tobacco (local lesion host) infected with TMV-Tomato. Systemic necrosis was induced in Xanthi-nc and KF 114 when infected with TMV-C at high temperature, but not with the other strains.

• Journal of the Korean Society of Tobacco Science
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• v.17 no.1
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• pp.41-48
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• 1995

Tobacco mosaic virus (TMV), TMV-common strain, causes severe damage on flue-cured tobacco in Korea. As the results, breeding programs were conducted to transfer the TMV resistance from NC567 to other cultivars. Advantage of F1, hybrid may not lie only in their heterotic value but also in directly combining of valuable dominant traits, e.g. for disease resistance, either in pairs or as groups already assembled in the two parents. A new TMV resistant flue-cured tobacco variety KF114 was developed from a cross as maternal parent MSNC567 and NC82 at the Suwon Experiment Station. KF114 was more vigorous growth characteristics in early stage than that of NC82, standard variety in Korea, and other characters were similar to those of NC82. It os high resistance to TMV, but bacterial wilt(Pseudomonas solanacerum) and black shank (Phytophthora parasitica var. nicotianae) is comparable to NC82. It should adapt well to the flue-cured production area and can reduce premature flowering under unfavorable weather conditions. Yield of KF114 is 1% to 5% higher, and nearly equal in value per KG compared with NC82. This variety, F1 hybrid, met acceptable standards for chemical and physical characteristics of cured leaf and high ranked good smoking taste by panel members in Korea Ginseng and Tobacco Research Institute.

• Journal of the Korean Society of Tobacco Science
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• v.22 no.1
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• pp.13-18
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• 2000

All steps of the curing process are automatically controlled by preseted program according to stalk positions in flue-cured tobacco. The bulk curing experiment was carried out to evaluate the effect of the basic and modified curing program in curing time schedule of two bulk models in physical properties of cured leaves in a new flue-cured tobacco variety KF 114(Wicotiano tabacum L.). The curing process of KF 114 was prolonged in yellowing and quicker in browning stage than those of NC 82. There was no significant difference in physical properties and chromatic characteristics of the cured leaves between basic and modified program at two bulk models. The ratio of normal leaf color tended to increase and the greenish leaf decreased in the modified curing program of two models, but no difference in the brownish leaf ratio was olserved between two programs.

• Proceedings of the Korean Society of Tobacco Science Conference
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• 1999.10a
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• pp.16-16
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• 1999

• Journal of the Korean Society of Tobacco Science
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• v.23 no.2
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• pp.162-167
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• 2001

This study was carried out to quantitatively determine phenol compounds in smoke total particulate matter(TPM) to evaluate the qualititive characteristics of flue-cured tobacco varieties(NC82, KFl14 and KFl18). After collecting The TPM by using smoking machine, the concentration of phenol compounds were analyzed by gas chromatography as their trimethylsilyl derivative on a fused capillary column bonded with a 5％ PE ME siloxane stationary phase. In all the flue-cured tobacco varieties, the major phenol components were monohydroxy compounds. The order of the highest concentration of total phenol compounds in TPM was NC82, KF114 and KFl18 but the contents of dihydroxy compound in the KFl18 was higher than those of NC82 and KF114.

• Journal of the Korean Society of Tobacco Science
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• v.23 no.2
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• pp.168-178
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• 2001

Essential oil in tobacco leaves influences the taste and aroma of cigarette smoke and is important to tobacco quality. This study was conducted to investigate the change in the level of essential oil components during flue-curing process of two flue-cured tobaccos, NC82 and KEl14. Flue-curing process was divided by six steps; harvest stage, the end of yellowing stage, the middle of color fixing stage, the end of color fixing stage, the middle of midrib drying stage, full-cured stage. NC82 in each stage contained 0.28%, 0.30%, 0.35%, 0.36%, 0.40% and 0.42% essential oil, respectively, and KF114 were 0.29%, 0.31%, 0.34%, 0.36%, 0.39% and 0.41%, respectively. Almost all hydrocarbons on the basis of relative peak area were gradually increased in two varieties with curing, neophytadiene content in them was highest at the full-cured stage. Most of alcohols and esters with curing showed a declining trend, but benzyl alcohol was increased in two tobaccos. Ketones were largely increased at the midrib drying stage during the curing process, especially, the most largely increasing constituent was $\beta$-damascenone among them. The content of 2-butylterahydrofuran, heterocyclic compounds, was largely increased at tile color fixing stage. There was no considerable difference between NC82 and KFl14 at the GC profile of essential oil and the pattern of each components during flue-curing process.

• Journal of the Korean Society of Tobacco Science
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• v.17 no.2
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• pp.114-119
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• 1995

Cultivars of Nicotiana tabacum L. normally have pink flowers, but the flue-cured tobacco mutant line, BU 8832-85, had white flower. The mutant line was crossed with five normal varieties of KF 109, NC 82, TC 499, NC 567 and Coker 176. All Fl plants showed pink flower. The progenies of F2 generations were segregated with the phenotypic ratio 9 : 3 : 4 with pink, varigated(a recombinant type) and white flower, respectively. Test-cross populations showed 1 : 1 : 2 ratios. These results showed that the white flower character was controlled by two recessive genes. The genes were designated as FFCC for pink and ffcc for white flower. The recessive gene ff was epistatic to C and c. Therefore, white flower had a recessive epistasis gene.

• Journal of the Korean Society of Tobacco Science
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• v.25 no.1
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• pp.47-52
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• 2003

The purposes of this study were to examine effect by Solid Matrix Priming(SMP) ; method far enhanced rapid and uniform seedling emergence in tobacco seeds. The results were as follows. No enhancement of germination rate was by SMP treatment. In KF114 during 9 days treatment and in KB108 during 7 days treatment, germinative energy was highest, and T50 and mean germination time were the shortest. Cells in embryo and hypocotyl were divided and extended during SMP treatment. The contents of fatty acid were increased in SMP treatment. The changes of glucose content were little.

• The Plant Pathology Journal
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• v.16 no.5
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• pp.264-268
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• 2000

A fluorescent material was accumulated in inoculated leaves showing necrotic local lesions of tobacco plants with N gene, Nicotiana tabacum cvs. Xanthi-nc NN, Samsun NN, Burley 21 and KF 114, and N. glutinosa, and Datura stramonium at the early growth stages by the inoculation of Tobacco mosaic virus (TMV). It was identified as a coumarin phytoalexin, scopoletin. Although the material was most prominently produced in TMV-inoculated tobacco leaves with local necrotic lesions, its accumulation was also noted in uninoculated leaves of TMV-inoculated plants. Its accumulation was somewhat greater in high resistance-induced leaves than low resistance-induced and intact leaves. Scopoletin treatment induced the expression of a pathogenesis-related protein, PR-1, prominently at the concentration of 500 or 1000 ${\mu}$g/ml. This suggests that scopoletin is a phytoalexin abundantly accumulating in N gene-containing resistant plants in response to TMV infection, and may be related to hypersensitive responses (HR) and systemic acquired resistance (SAR) in the resistant tobacco plants.

• Proceedings of the Korean Society of Tobacco Science Conference
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• 2001.05a
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• pp.35-43
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• 2001

일대잡종육종이란 현대의 다양한 육종기술 중 하나로 교배 제1세대의 잡종강세현상을 곧바로 이용하는 것으로 여러 작물에서 실용적으로 이용되고 있다. F$_1$잡종종자를 생산하려면 자가수분을 통제해야 하는데 웅성불임을 이용하면 자친의 화분제거 노력이 생력되어 경제적인 종자생산이 가능하다. 연초는 자식성 작물로 잡종강세는 높지 않으나 종간 교잡에 의한 cms가 만들어진 이후 버어리종 등에서는 60년대부터 직적형질에 가깝게 유전되는 우성 단일유전자의 지배를 받는 N. logniflora의 역병(race 0)저항성을 도입한 웅성불임(cms) F$_1$잡종이 활발하게 이용되어 왔다. 최근에는 cms F$_1$잡종의 이용영역이 넓어져 우리나라의 경우 N. glutinosa에서 유래한 우성 단일인자 TMV저항성을 도입한 황색종 cms F$_1$잡종 KF 114가 주 재배품종이 되었고 버어리종에서는 VAM에서 유래한 열성단일유전자의 지배를 받는 PVY저항성이 도입된 KB 111의 확대재배가 예상되고 있다. 본 글에서는 cms F$_1$잡종의 국내외 이용 사례를 살펴보고 cms F$_1$잡종의 육성과 이용방향, cms F$_1$잡종 종자생산에 관하여 기술하였다. 그리고 병저항성뿐 아니라 알칼로이드함량, 염록소함량, DVT나 챤-abienol 및 SE함량 등 질적형질에 가깝게 유전되고 유전양식이 명확하게 밝혀진 화학성분에 대해서도 F$_1$잡종 육성의 대상 형질로 논의하였다.