• Title/Summary/Keyword: KD-$(k_0,\

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Preparation and Characterization of the Hydrolyzed Protein from Shaving Scraps of Leather Waste Containing Chromium (피혁폐기물(皮革廢棄物)인 Shaving scraps으로 부터 가수분해(加水分解) 단백질(蛋白質)의 제조(製造) 및 특성(特性))

  • Kim, Won-Ju;Cho, Ju-Sik;Lee, Hong-Jae;Heo, Jong-Soo
    • Journal of the Korea Organic Resources Recycling Association
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    • v.5 no.2
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    • pp.47-56
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    • 1997
  • To examine of possibility protein recycling of shaving scraps contained chrome generated from manufacturing process of leather, the characteristics of hydrolyzed protein that differently treated with MgO as alkaline agent were investigated. In alkaline hydrolysis of saving scraps treated with MgO, MgO had to be treated over 5.0% to maintain over pH 8.0 that is insoluble of chrome. Under the condition of alkaline treated with MgO, the solubility of chrome is low with about 60%. The average molecular weight of hydrolyzed proteins from shaving scraps treated with MgO was about 80~100 KD. The amino acid contents of that were largely collagen proteins such as glycine, alanine and proline, and acidic amino acids such as aspartic acid and glutatamic acid. The contents of Mg, Ca and Na in hydrolyzed protein were too much as liquid fertilizer, and chrome contents was 30~40 ppm that largely decreased in comparing with raw materials (40,000~42,000 ppm).

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Liver Plasma Membrane and Nuclear $T_{3}$ Receptor Binding in the Obese (ob/ob) Mouse (비만 쥐(ob/ob mouse)의 간 세포막과 핵에 있는 $T_{3}$ 수용체의 결합능력에 관한 연구)

  • Kim, Kyung-Ah;Lachance, Paul A.
    • Journal of Nutrition and Health
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    • v.24 no.4
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    • pp.356-365
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    • 1991
  • $L-Triiodothyronine(T_3)$ binding to purified plasma membrane and to isolated nuclei from the same liver in obese(ob/ob) mice and their lean littermates was examined. The maximal binding capacity(Bmax) for $T_3$ receptor of liver nuclei, as compared to lean control, was significantly lower in the obese mouse$(obese 527{\pm}80fmol/mg\;DNA ; lean 883{\pm}62fmol/mg\;DNA)$, without an apparent difference in dissociation constant(Kd). The finding that obese mice have fewer liver nuclear $T_3$ receptors confirms previous reports. The Bmax and Kd of liver plasma membrane $T_3$ receptor were not significantly different between obese and lean mouse, which suggests no defect to be occurring in the function of the plasma membrane $T_3$ receptor and reinforces the view that the peripherally impaired thyroid hormone action in obese mice is a post plasma membrane receptor event. These results further support the hypothesis that the major defect of the thyroid hormone metabolism in genetic obesity occurs at the level of the nuclear receptor.

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Treatability Study on the SepticTAnk Sludges

  • Byung Soo Yang
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.17 no.1
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    • pp.41-47
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    • 1981
  • The characteristics of septic tank sludges were investigated and the kinetic coefficients in the aerobic biodegradation were evaluated from bach treatability tests. Using an unbiased statistical method, the estimated values, k (substrate removal rate coefficient) =0. 0175hr-1 at 17\ulcornerC, K. (Michaelis Menten constant) = 248mg/ e, a (cell yield coefficient)=0.625, and Kd (cell decay coefficient:' =0. 00192hr-1 were obtained based on biodegradable COD(mg/ \ulcorner) and volatile suspended solids(mg/\ulcorner). The relationship between COD and BOD, COD (mg/\ulcorner) =2. 1 BOD(mg/\ulcorner) +250, also was established for the septic tank sludges. Dilution was inevitable for the grit removal because of the high viscosity of the sludges. An aerobic activated sludge process rather than anaerobic processes was recommended for the removal of soluble organics after the removal of grit and suspended solids. A multi-stage activated sludge process was adapted for this highly concentrated and not easily-degradable waste. It was estimated that a four-stage activated sludge process would require 40 hours retention time compared to 92 hours for a single-stage process, 52 hours for a double-stage process, and 46 hours for a three stage process in order to achieve an effluent quality of 84mg/ e COD( 40mg/ e BOD) with about 4, OOOmg/ \ulcorner MLSS from an influent quality of I, 500mg/ t COD(714mg/.e BOD), while multi-stages beyond four stage would not save the required retention time significantly.

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The Impact of Service Quality on Customer Satisfaction, Service Value, and Store Loyalty in a University-Based Convenience Store

  • Kim, Jong-Lak;Lee, Young-Chul;Han, Sang-Ho;Lim, Su-Ji
    • Journal of Distribution Science
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    • v.11 no.5
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    • pp.5-15
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    • 2013
  • Purpose - The purpose of this study is to investigate the impact of the service quality of a university-based convenience store on consumer satisfaction, service value, and customer loyalty. Research design, data, and methodology - The questionnaire was developed by using the modified and supplementary questions based on the KD-SQS model. We used the SPSS/PC 18.0 and AMOS 18.0 statistical packages to analyze the results. For validating the research hypothesis and the structural relationship of the research model, path analysis was used. Results - The overall results of this study are as follows. We found that benefits, promotion, and convenience had a significant impact on two variables: customer satisfaction and service value. Conclusions - The basic benefits, promotions, and convenient facilities in the university-based convenience store have already received favorable reviews. Therefore, for improving customer satisfaction, it is important to improve the reliability of service, quality of human interaction, and customer service.

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Study on Thermodynamics of Three Kinds of Benzindocarbocyanine Dyes in Aqueous Methanol Solution

  • Huang, Wei;Wang, Lan-Ying;Fu, Yi-Le;Liu, Ji-Quan;Tao, You-Ni;Fan, Fang-Li;Zhai, Gao-Hong;Wen, Zhen-Yi
    • Bulletin of the Korean Chemical Society
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    • v.30 no.3
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    • pp.556-560
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    • 2009
  • Aggregation behavior of three kinds of benzindocarbocyanine dyes in aqueous methanol solution was studied by UV-Vis absorption spectrum. The results indicated that the three dyes all existed monomer-dimer equilibrium in aqueous methanol solution (concentration range $10^{−5}\;to\;10^{−6}$ M) at 25.0$\sim$41.0 ${^{\circ}C}$ for Dye 1, 28.0$\sim$49.0 ${^{\circ}C}$ for Dye 2 and 26.0$\sim$47.0 ${^{\circ}C}$ for Dye 3. The fundamental property of the three dyes as the dimeric association constant KD, the dimeric free energy ${\Delta$}G_D, the dimeric entropy ${\Delta$}S_D, and the dimeric enthalpy ${\Delta$}H_D were determined. The ${\Delta$}H_D of three dyes: Dye 1, Dye 2 and Dye 3 was -42.5, -15.1 and -18.9 kJ/mol, respectively. The experimental observations were the subject of a theoretical study including the ground-state geometries which were fully optimized using DFT at B3LYP/6-31G level. The effect of dye molecule structure on ${\Delta$}H_D was discussed by theoretical calculations.

Adsorption and Movement of Fenoxaprop-P-ethyl in Soils (토양중 fenoxaprop-P-ethyl의 흡착성 및 이동성)

  • Han, Soo-Gon;Ahn, Byung-Koo;Moon, Young-Hee
    • Korean Journal of Weed Science
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    • v.18 no.4
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    • pp.325-332
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    • 1998
  • This study was carried out to investigate the adsorption and the movement of herbicide fenoxaprop-P-ethyl in the silty clay soil(SiC) and the sandy loam soil(SL). Fifteen percent of the added herbicide was adsorbed within 30 min after shaking, and a quasi-equilibrium was reached after 8 to 14 h. The time required for 50% adsorption was 15.8 h in the SiC and 19.3 h in the SL. The equilibrium adsorption isotherm was followed by the Freundlich equation and the Kd was 3.86 in the SiC and 2.32 in the SL. The herbicide in the soil columns flooded with 3 cm water depth and eluted at 0.8 cm/day was leached to 6 cm and 8 cm depth at 7 and 21 days after the treatment, respectively. However, the movement was widened with increased amount of leaching water. The herbicide in the field soils was moved up to 6 cm and 8 cm depth at 14 and 56 days after the treatment, respectively. However, the large amount of the applied herbicide was distributed in 0~2 cm profile in all of the soils examined. Half-life of the chemical in soils was shorter than 7 days and the time to 90% degradation was about 4 weeks. The results indicate that the herbicide has relatively small mobility and short persistence.

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Behaviors of the Fungicide Procymidone in Soils (살균제 Procymidone의 토양 중 동태)

  • Choi, Gyu-Il;Seong, Ki-Yong;Kim, Jeong-Gyu
    • Korean Journal of Environmental Agriculture
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    • v.24 no.2
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    • pp.123-131
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    • 2005
  • This study was focused on adsorption, leaching, photolysis, and hydrolysis of the fungicide procymidone in soils. Adsorption type of procymidone on three different soil were well fitted to Linear and Freundlich isotherm. Distribution coefficients (Kd) were ranged from 2.75 to 12.18 and Freundlich isotherm Kf value $1.99{\sim}9.98$, 1/n value $0.74{\sim}0.89$. Desorption rates were $20.1{\sim}34.0%$ (Namgye), $26.3{\sim}44.6%$ (Jigog) and $31.6{\sim}50.9%$ (Baegsan series) and desorption hysteresis were $0.65{\sim}0.79,\;0.55{\sim}0.73\;and\;0.49{\sim}0.68$. Procymidone seemed to be stable to photolysis in acidic and neutral solutions but hydrolyzed rapidly in alkaline solution. Considering leaching properties procymidone mobility low in soils.

Runoff and Erosion of Alachlor, Ethalfluralin, Ethoprophos and Pendimethalin by Rainfall Simulation (인공강우에 의한 alachlor, ethalfluralin, ethoprophos 및 pendimethalin의 토양표면 유출)

  • Kim, Chan-Sub;Ihm, Yang-Bin;Lee, Young-Deuk;Oh, Byung-Youl
    • Korean Journal of Environmental Agriculture
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    • v.25 no.4
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    • pp.306-315
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    • 2006
  • Two different experiments, adsorption/desorption and runoff by rainfall simulation of four pesticides, such as alachlor, ethalfluralin, ethoprophos and pendimethalin were undertaken their runoff and erosion losses from sloped land and to assess the influence of their properties and environmental factors on them. The mobility of four pesticides and which phase they were transported by were examined in adsorption study, and the influence of rainfall pattern and sloping degree on the pesticide losses were evaluated in simulated rainfall study. Freundlich adsorption parameters (K) by the adsorption and desorption methods were 1.2 and 2.2 for ethoprophos, 1.5 and 2.6 for alachlor, respectively. And adsorption distribution coefficients (Kd) by the adsorption and desorption methods were 56 and 94 for ethalfluralin, and 104 and 189 for pendimethalin, respectively. K or Kd values of pesticides by the desorption method which were desorbed from the soil after thoroughly mixing, were higher than these ones by the adsorption method which pesticides dissolved in water were adsorbed to the soil. Another parameter (1/n), representing the linearity of adsorption, in Freundlich equation for the pesticides tested ranged from 0.96 to 1.02 by the desorption method and from 0.87 to 1.02 by the adsorption method. Therefore, the desorption method was more independent from pesticide concentration in soil solution than the adsorption method. By Soil Survey and Land Research Center (SSLRC)'s classification for pesticide mobility, alachlor and ethoprophos were classified into moderately mobile $(75{\leq}Koc<500)$, and ethalfluralin and pendimethalin were included to non-mobile class (Koc > 4000). Runoff and erosion loss of pesticides by three rainfall scenarios were from 1.0 to 6.4% and from 0.3 to 1.2% for alachlor, from 1.0 to 2.5% and from 1.7 to 10.1% for ethalfluralin, from 1.3 to 2.9% and from 3.9 to 10.8% for pendimethalin, and from 0.6 to 2.7% and from 0.1 % 0.3% for ethoprophos, respectively. Distribution of pesticides in soil profile were investigated after the simulated rainfall study. Alachlor and ethoprophos were leached to from 10 to 15 cm of soil layer, but ethalfluralin and pendimethalin were mostly remained at the top 5 cm of soil profile. The losses of the pesticides at 30% of sloping degree were from 0.2 to 1.9 times higher than those at 10%. The difference of their runoff loss was related with their concentration in runoff water while the difference of their erosion loss must be closely related with the quantity of soil eroded.

Free-strain solutions for two-dimensional consolidation with sand blankets under multi-ramp loading

  • Zan Li;Songyu Liu;Cuiwei Fu
    • Geomechanics and Engineering
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    • v.35 no.4
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    • pp.385-393
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    • 2023
  • To analyze the consolidation with horizontal sand drains, the plane strain consolidation model under multi-ramp loading is established, and its corresponding analytical solution is derived by using the separation of variables method. The proposed solution is verified by the field measurement data and finite element results. Then, the effects of the loading mode and stress distribution on consolidation and dissipation of pore pressure are investigated. At the same time, the influence of hydraulic conductivity and thickness of sand blankets on soil consolidation are also analyzed. The results show that the loading mode has a significant effect on both the soil consolidation rate and generation-dissipation process of pore water pressure. In contrast, the influence of stress distribution on pore pressure dissipation is obvious, while its influence on soil consolidation rate is negligible. To guarantee the fully drained condition of the sand blanket, the ratio of hydraulic conductivity of the sand blanket to that of clay layer kd/kv should range from 1.0×104 to 1.0×106 with soil width varying from 100 m to 1000 m. A larger soil width correspondingly needs a greater value of kd/kv to make sure that the pore water can flow through the sand blanket smoothly with little resistance. When the soil width is relatively small (e.g., less than 100 m), the effect of thickness of the sand blanket on soil consolidation is insignificant. And its influence appears obvious gradually with the increase of the soil width.

Analysis of Clonorchis sinensis antigens and diagnosis of clonorchiasis using monoclonal antibodies (단세포군 항체를 이용한 간흡충 항원의 분석 및 간흡충증의 진단)

  • Yong, Tae-Sun;Im, Gyeong-Il;Jeong, Pyeong-Rim
    • Parasites, Hosts and Diseases
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    • v.29 no.3
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    • pp.293-310
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    • 1991
  • Clonorchis sinensis is a common parasite of man in Korea. Researches on the specific antigens of C. sinensis would be valuable not only because those elucidate the molecular characteristics of this fluke but also because it is applicable to immunodiagnosis. Although many monoclonal antibodies have been used in the field of parasite immunology, few articles on monoclonal antibodies against C. sinensis have been published so far. The aim of this study was to analyse C. sinensis antigens recognized by monoclonal antibodies, and to set up ELISA-inhibition test using C. sinensis specific monoclonal antibodies for improved specificity of immunodiagnostic tests. By fusion between spleen cells of the mice immunized with C. sinensis water-soluble crude adult worm antigens and plasmacytoma cells of mouse origin, 29 hybridoma clones secreting anti-C. sinensis monoclonal antibodies were made, and 8 clones among those were found specific. After cell cloning, isotypes of 6 selected specific monoclonal anti- bodies were determined to be IgGl, IgG2b and IgA. Four exposed antigenic determinants of natural infection were recognized by different specific monoclonal antibodies. By enzyme-immunoelectrotransfer blot, 10 KD, 34 KD antigenic determinants were found to be reacted with CsHyb 0714-20, CsHyb 0605-10 monoclonal antibodies, respectively, The antigenic determinant recognized by CsHyb 0714-20 monoclonal antibody was revealed to be located at the surface and parenchyme of a parasite by indirect immunoauorescent antibody technique, and those reacted with CsHyb 0605-10, CsHyb 0714-25 monoclonal antibodies were found at the parenchyme and intestine. The antigenic determinant reacted with CsHyb 0605-23 monoclonal antibody was found mainly around the uterine eggs. Four antigenic determinants recognized by specific monoclonal antibodies were all found to be present in the early eluted fractions of C. sinensis antigens separated by Sephadex G-200 gel filtration. By conventional ELISA, 75% of clonorchiasis cases were found positive, but 7.1% of normal controls and 37.5% of paragonimiasis cases showed false positives. However, by ELISA-inhibition test using C. sinensis specific monoclonal antibody (CsHyb 0605-23), 77.1% of clonorchiasis cases were found positive, and there were no false positives in normal controls or paragonimiasis cases, indicating 100% specificity. The ELISA- inhibition test using monoclonal antibodies was found to have same sensitivity and definitely high specificity in comparison with conventional ELISA for serodiagnosis of human clonorchiasis.

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