• Title/Summary/Keyword: K-40

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Characterization of B Cells of Lymph Nodes and Peripheral Blood in a Patient with Hyper IgM Syndrome (Hyper IgM Syndrome 환자에서 얻은 림프절 및 말초혈액 B세포의 특성)

  • Kim, Dong Soo;Shin, Kyuong Mi;Yang, Woo Ick;Shin, Jeon-Soo;Song, Chang Hwa;Jo, Eun Kyeong
    • Clinical and Experimental Pediatrics
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    • v.46 no.2
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    • pp.128-136
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    • 2003
  • Purpose : Hyper IgM syndrome(HIGM) is characterized by severe recurrent bacterial infections with decreased serum levels of IgG, IgA, and IgE but elevated IgM levels. Recently, it has been classified into three groups; HIGM1, HIGM2 and a rare form of HIGM. HIGM1 is a X-linked form of HIGM and has now been identified as a T-cell deficiency in which mutations occur in the gene that encodes the CD40 ligand molecule. HIGM2 is an autosomal recessive form of HIGM. Molecular studies have shown that the mutation of HIGM2 is in the gene that encodes activation-induced cytidine deaminase(AID). Recently, another rare form of X-linked HIGM syndrome associated with hypohydrotic ectodermal dysplasia has been identified. We encountered a patient with a varient form of HIGM2. To clarify the cause of this form of HIGM, we evaluated the peripheral B cells of this patient. Methods : The lymphocytes of the patient were prepared from peripheral blood. B cells were immortalized with the infection of EBV. Cell cycle analysis was done with the immortalized B cells of the patient. Peripheral mononuclear cells were stained with monoclonal anti-CD40L antibody. Total RNA was extracted from the peripheral mononuclear cells. After RT-PCR, direct sequencing for CD40L gene and HuAID gene were done. Immunostainings of a lymph node for CD3, CD23, CD40, Fas-L, bcl-2, BAX were done. Results : The peripheral B cells of this patient showed normal expression of CD40L molecule and normal sequencing of CD40L gene, and also normal sequencing of AID gene. Interestingly, the peripheral B cells of this patient showed a decreased population of G2/mitosis phase in cell cycles which recovered to normal with the stimulation of IL-4. Conclusion : We suspect that the cause of increased serum IgM in this patient may be from a decrease of G2/mitosis phase of the peripheral B cells, which may be from the decreased production or secretion of IL-4. Therefore, this may be a new form of HIGM.

Reduction of Magnet Eddy Current Loss in 40kW HEV Traction motor by using FEA method (FEA 기법을 이용한 40kw급 HEV용 Traction 모터 Magnet Eddy Current Loss 저감 설계)

  • Kim, Kyung-Su;Lee, Sung-Ho;Cha, Hyun-Rok;Park, Sung-Jun;Lee, Kyu-Seok;Jeon, Seung-Kyu
    • Proceedings of the KIEE Conference
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    • 2008.07a
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    • pp.820-821
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    • 2008
  • 본 논문은 FEA 기법을 이용하여 40kW급 HEV용 Traction 모터의 설계 및 고효율화를 위한 회전자 영구자석의 Eddy Current loss 저감 방안에 대한 연구를 수행하였다. 먼저 FEA 기법을 이용하여 40kW급 HEV용 Traction 모터 설계 및 특성해석을 수행하여 FEA 해석기법의 타당성을 확보하였다. 또한 Traction 모터의 손실 저감을 위해 magnet부분의 eddy current loss 저감방안에 대해 논하였으며, FEA 기법을 이용하여 회전자 magnet이 Solid, 1/2, 1/4, 1/14 segments로 나눈 타입에 따른 결과를 비교 분석하였다. 그 결과 magnet형태를 기존의 Solid 타입에서 14segments 타입으로 분할 시, magnet 내부의 current path가 줄어들어 eddy current loss가 가장 많이 저감됨을 알 수 있었으며, 이를 통해 HEV용 traction 모터의 고효율, 고성능화 설계방안을 도출 할 수 있었다.

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A Stable 40 GHz Pulse Train Generation by Pulse Repetition-Frequency Quadruplication Using a Fiber Fabry-Perot Interferometer

  • Ruan, Wan-Yong;Park, Jae-Hyun;Seo, Dong-Sun
    • Journal of IKEEE
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    • v.12 no.4
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    • pp.234-238
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    • 2008
  • We demonstrate a simple method to generate a stable 40 GHz pulse train at 1550 nm by spectral filtering of a 10 GHz mode.locked pulse source using a fiber Fabry-Perot interferometer (FFPI). A high finesse FFPI with a 40 GHz free spectral range blocks successfully unwanted spectral components of a 10 GHz pulse source and passes only 40 GHz spaced spectral lines ensuring pulse repetition-frequency quadruplication of the input pulses.

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10bits 40MS/s $0.13{\mu}m$ Pipelined A/D Converter for WLAN (WLAN용 10비트 40MS/s $0.13{\mu}m$ 파이프라인 A/D 변환기)

  • Park, Hyun-Mook;Cho, Sung-Il;Yoon, Kwang-Sub
    • Proceedings of the IEEK Conference
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    • 2008.06a
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    • pp.559-560
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    • 2008
  • In this paper, I proposed 10bits 40MS/s Pipelined A/D converter. The op-amps for SHA and MDAC designed folded-cascode amplifier with gain-booster. And the MOS transistors with a low threshold voltage are employed to low on-resistor and parasitic capacitance. The power dissipation is 119㎽ at 1.2V and 40MS/s

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EARLY SCREENING OF EXPRESSION OF SV40 DRIVEN LACZ INTRODUCED INTO BOVINE EMBRYOS

  • Nakamura, A.;Okumura, J.;Muramatsu, T.
    • Asian-Australasian Journal of Animal Sciences
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    • v.8 no.5
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    • pp.449-454
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    • 1995
  • The present study was conducted to assess gene expression of bacterial lacZ driven by the SV40 promoter at early developmental stages of bovine embryos. The lacZ gene was linearized with BamHI digestion and introduced into the pronucleus by microinjection at 20 hrs after the commencement of in vitro fertilization. Intact bovine blastocysts were not stained with X-Gal, suggesting that there is no endogenous beta-galactosidase activity in these blastocysts. In contrast, the bovine blastocyst cells microinjected with the lacZ gene exerted a characteristic greenish-blue color originating from the bacterial beta-galactosidase activity, albeit at a low rate, i.e. 2.1% of the total fertilized oocytes injected. It was concluded, therefore, that the lacZ gene driven by the SV40 promoter could be used for an indirect screening method in which the presence of transgene is evaluated from the product of transgene expression.

Regioselective Synthesis of Z-Aldoximes Catalyzed By H3PMo12O40 under Solvent-Free Conditions (Solvent-free 조건하에서 H3PMo12O40 촉매에 의한 Z-Aldoximes의)

  • Eshghi, Hossein;Alizadeh, Mohammad Hasan;Davamdar, Ehsan
    • Journal of the Korean Chemical Society
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    • v.52 no.1
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    • pp.52-56
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    • 2008
  • facile and efficient method for the preparation of Z-aldoximes is improved by means of H3PMo12O40 catalyst in solvent-free media. The major advantages of this method are: operational simplicity, low catalyst loading, selectivity, mild reaction conditions, short reaction times and excellent yields. The recovered catalyst could be used in new attempts without any purification.