• Title/Summary/Keyword: K-12

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Fibrinolytic Activity and Characterization of Bacillus licheniformis HK-12 Isolated from Chungkook-Jang (청국장에서 분리한 세균인 Bacillus licheniformis HK-12의 혈전용해활성 및 특징)

  • Sohn, Byung-Hee;Song, Yu-Jin;Oh, Kye-Heon
    • KSBB Journal
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    • v.23 no.3
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    • pp.251-256
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    • 2008
  • The aim of this work was to investigate the fibrinolytic activity and characterization of Bacillus licheniformis HK-12, which produces the fibrinolytic enzyme excreted from naturally fermented Chungkook-Jang. Initially, the physiological and biochemical characteristics of strain HK-12 was examined. Both physiological analysis using BIOLOG system and phylogenetic analysis using 16S rRNA sequencing were performed to identify the strain, and the strain could be assigned to Bacillus licheniformis, designated as B. lichenformis HK-12, and registered in GenBank as [EU288193]. Phylogenetic analysis of B. licheniformis HK-12 was plotted based on 16S rRNA sequence comparisons. During the incubation period of B. licheniformis HK-12, the changes of bacterial growth, fibrinolytic activity, and pH were monitored. As the results, after 36 hours of incubation, the maximum fibinolytic activity was about 2.25 times than that of plasmin used as standard. Optimal conditions on the growth of B. licheniformis HK-12 associated with the fibrinolytic activity was initial pH 7.0 and 40$^{\circ}C$, respectively.

Comparison of anthropometric data and clinical health indicators according to the serum vitamin $B_{12}$ status in female older adults living in a rural area of Jeonla province, Korea (전라도 일부농촌지역 여성 노인의 혈중 비타민 $B_{12}$ 수준에 따른 신체계측치 및 임상건강지표들의 비교)

  • Kwak, Chung Shil;Cho, Ji Hyun;Yon, Miyong
    • Journal of Nutrition and Health
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    • v.46 no.3
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    • pp.239-249
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    • 2013
  • Subclinical vitamin $B_{12}$ deficiency is common in the elderly worldwide. We investigated the change of serum vitamin $B_{12}$ concentration with aging and compared anthropometric data and clinical health indicators between normal (${\geq}$ 340 pg/mL) and low (< 340 pg/mL) serum vitamin $B_{12}$ groups in 470 Korean women aged 65 years and over living in a rural area. Serum vitamin $B_{12}$ concentration showed inverse correlation with age (r = -0.0992, p < 0.05). The normal $B_{12}$ group showed significantly (p < 0.05) higher red blood cell count, hemoglobin, and hematocrit compared to the low $B_{12}$ group, however, no difference in mean corpuscular volume was observed between the two groups. The normal $B_{12}$ group showed significantly lower serum homocysteine concentration (p < 0.01) and prevalence of vitamin D (p < 0.01) or folate deficiency (p < 0.001). Bone mineral density (T-score) was significantly higher (p < 0.05) in the normal $B_{12}$ group, compared with that in the low $B_{12}$ group, and showed positive correlation (r = 0.1490, p < 0.01) with serum vitamin $B_{12}$ concentration after adjusting for age, body weight, and body mass index. No differences in anthropometric data, physical activity, and smoking and drinking habits were observed between the two groups. In conclusion, it could be suggested that older female adults with normal serum vitamin $B_{12}$ level would be less anemic and osteoporotic and more resistant to hyperhomocysteinemia associated chronic diseases than those with low serum vitamin $B_{12}$ level.

[ β ]-Lactamase Inhibitory Activities of New 6-tricyclic Substituted Exomethylene Penam Sulfones

  • Lee, Su-Jin;Kim, Hyun-Jin;Sheen Yhun Y.;Lee, Kwan-Soon;ParkChoo, Hea-Young
    • Biomolecules & Therapeutics
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    • v.14 no.4
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    • pp.220-225
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    • 2006
  • Derivatives of penicillanic acid sulfones are known to be irreversible inhibitors of $\beta$-lactamase. Eight 6-tricyclic methylene penicillanic acid sulfones were prepared, and their $\beta$-lactamase inhibitory activities were evaluated against $\beta$-lactamase types I, II, III and IV. Among the tricycles attached to 6-exomethylenepenam sulfones, thiazolobenzimidazole(12a-12b), fluorene(12c), and carbazole(12e), showed inhibitory activity on type I, II and III $\beta$-lactamase. But phenanthrene(12d), and anthracene(12f-12h) derivatives showed little $\beta$-lactamase inhibitory activity. The synergic effects of the selected compound(l2b) in 1:4 combination with piperacillin showed some protection to piperacillin for the resistant strains of E. coli DC2 and P. aeruginosa 1771.

Effects of Moxibustion at Combined Acupoints of ST36, BL21 and CV12 on Small Intestinal Motility for Sex and Ages of Rats (흰쥐의 성별과 주령에 따른 족삼리(足三里) 위유(胃兪) 중완(中脘) 배혈(配穴) 시구(施灸)가 소장(小腸) 수송능에 미치는 효과)

  • Yu, Yun-Cho;Kwon, O Sang;Oh, In-Kyun;Yang, Seung Bum;Ahn, Seong Hoon;Sohn, In Chul;Kim, Jae Hyo
    • Korean Journal of Acupuncture
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    • v.29 no.4
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    • pp.604-615
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    • 2012
  • Objectives : The aim of this study was to observe the effect of moxibustion at combined acupoints of ST36, BL21 and CV12 in rats with sex and age. Methods : This study measured small intestinal motility in rats. First, cauterize with moxa was applied 5 times to the ST36, BL21 and CV12 in rats classified by sex and age under enflurane anesthesia. And then same treatment is done to mached acupoints of ST36, BL21 and CV12. Results : In single acupoint groups, cauterizing with moxa on ST36 increased with 5, 7 weeks in female and all weeks in male. The BL21 group increased with 5, 6, 7 weeks in female, and only 5 weeks in male. The CV12 group showed increasing with 5, 6 weeks in female and only 7 weeks in male. In combined acupoints groups, the ST36+BL21 with 8 weeks in male, the ST36+CV12 with 5, 8 weeks in female and 5, 6, 8 weeks in male, the CV12+BL21 only with 5 weeks in female, the ST36+BL21+CV12 only with 5 weeks in female showed increasing, respectively. Conclusions : Although these differents according to the sex and age in rats do not have a established tendency, the results suggested that the effects of moxibustion are experimentally should be considered the sex and age of individual.

Synthesis and Magnetic Relaxation of [Mn12O12(O2CCH2CH2CH2Cl)16(H2O)4] Complex

  • Jeon, Won-Suk;Jin, Mi-Kyung;Kim, Yoo-Jin;Jung, Duk-Young;Suh, Byoung-Jin;Yoon, Seok-Won
    • Bulletin of the Korean Chemical Society
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    • v.25 no.7
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    • pp.1036-1040
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    • 2004
  • $Mn_{12}O_{12}(O_2CCH_2CH_2CH_2Cl)_{16}(H_2O)_4]$ (noted as $Mn_{12}$-BuCl), a new polynuclear complex of manganese chlorobutyrate has been successfully prepared by substitution of acetate with 4-chlorobutyric acid. The $Mn_{12}-BuCl$ crystallizes into triclinic space group P-1 with a = 14.5560(11) ${\AA}$, b = 14.5819(11) TEX>${\AA}$, c = 27.265(2) ${\AA}$, ${\alpha}\;=\;84.1140(10)^{\circ}\;,\;{\beta}\;=\;88.805(2)^{\circ},\;{\gamma}\;=\;89.8820(10)^{\circ}$, and Z = 2. The local environments of manganese 3+ and 4+ ions of the title compound are close to those of other $Mn_{12}$ compounds. The electrochemical data for $Mn_{12}-BuCl$ involve reversible reactions of two-electron reductions. The $Mn_{12}-BuCl$ also presents magnetic relaxation below 10 K implying that each molecule behaves as a single molecule magnet.

275 GHz 이상 전파천문대역 보호를 위한 APG-12 5차회의 최종결과

  • Chung, Hyun-Soo;Je, Do-Heung;Oh, Se-Jin;Roh, Duk-Gyoo;Sohn, Bong-Won;Lee, Sang-Sung;Kim, Hyo-Ryoung
    • The Bulletin of The Korean Astronomical Society
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    • v.36 no.2
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    • pp.134.1-134.1
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    • 2011
  • APT(Asian-Pacific Telecommunity)는 아시아-태평양지역 국가의 공동이익을 도모하기 위하여 결성된 아-태지역 전파협의체 조직으로, WRC(세계전파통신회의)회의에서의 아-태 지역 주관청의 공동이익을 얻기 위해 APG(APT Conference Preparatory Group for WRC)회의를 연간 1회의 비율로 개최하고 있다. 2012년의 WRC-12회의에 대비하여 아태지역 회원국의 최종 공동의견서를 작성하기 위해, APG-12 5차회의가 2011년 8월 29일-9월 3일 부산에서 개최되었으며, 전파천문업무 보호 및 22 GHz 대역 달탐사, 38 GHz 대역 Space-VLBI운용대역과 관련된 WRC-12 의제 1.6, 1.11, 1.12 등에 대한 회의가 있었다. WRC-12 의제 1.6은 수동업무(전파천문업무, 지구탐사위성 및 우주연구업무)를 능동업무의 유해혼신으로부터 보호하기 위하여 제정한 전파규칙 각주 5.565조의 개정 검토와 관련된 의제이다. 따라서 한국천문연구원에서는 275 GHz 이상 대역에서 전파천문업무의 원활한 운용을 위해, APG-12 5차회의에 참석하여 WRC-12회의에 제출하기 위한 아-태지역 국가의 공동의견서 작성에 주도적인 역할을 담당하였다. 본 발표에서는 APG-12 5차 회의에 제안한 우리나라의 기고서 제안 내용 및 동 회의에서 의 최종 아태지역 회원국 공동의견서 결과 및 향후 대응책에 대해서도 알아보고자 한다.

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Development of Battery Equalizer for Commercial Vehicle (상용차용 배터리 이퀄라이저 개발)

  • Choi, Deok-Kwan;Kim, Jong-Cheol;Kim, Ho-Sik;Park, Jae-Kyu
    • Proceedings of the KIPE Conference
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    • 2008.06a
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    • pp.342-344
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    • 2008
  • 24V DC power system of commercial vehicle includes 28V alternator and 24V battery bank (two 12V battery bank in series). BEQ(Battery Equalizer) provides efficient and reliable 12V DC power to 12V electrical load in 24V DC power system. In addition to providing 12V DC power, BEQ ensures that battery voltages remain equal which extends battery life. It is the most cost effective and efficient solution for dual voltage systems. BEQ made by Hyundai MOBIS is specifically designed to provide reliable 12V DC power for 12V ABS(Anti-lock Brake system).

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A Characteristic Analysis of Four-Phase 16/12 SRM (4상 16/12극 SRM의 특성해석)

  • Song Hyun-Soo;Lee Dong-Hee;Ahn Jin-Woo;Hao Chen
    • Proceedings of the KIPE Conference
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    • 2002.07a
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    • pp.415-418
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    • 2002
  • In the paper, a four-phase 16/12 structure Switched Reluctance motor drive is presented. The construction of the stator and the rotor in the motor, the scheme of the rotor position detector and the main circuit of the power converter are described. The comparison of the four-phase 16/12 motor and the four-phase 816 motor and the comparison of the four-phase 16/12 motor and the three-phase 12/8 motor are made. In the controller, the PWM control variable-speed control, the commutation control, the four quadrants control, the overvoltage protection, the overcurrent protection and the under voltage protection could be achieved. Tested results of the developed prototype are made.

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Low Expression of Tyrosine-protein Phosphatase Nonreceptor Type 12 is Associated with Lymph Node Metastasis and Poor Prognosis in Operable Triple-negative Breast Cancer

  • Wu, Min-Qing;Hu, Pan;Gao, Jie;Wei, Wei-Dong;Xiao, Xiang-Sheng;Tang, Hai-Lin;Li, Xing;Ge, Qi-Dong;Jia, Wei-Hua;Liu, Ren-Bin;Xie, Xiao-Ming
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.1
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    • pp.287-292
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    • 2013
  • Background: Low tyrosine-protein phosphatase nonreceptor type 12 (PTPN12) expression may be associated with breast cancer growth, proliferation, and metastasis. However, the prognostic value of PTPN12 in breast cancer has not been clearly identified. Patients and Methods: 51 triple-negative breast cancer (TNBC) patients and 83 non-TNBC patients with a histopathology diagnosis from October 2001 to September 2006 were included in this study. Immunohistochemical staining for PTPN12 on tissue microarrays was conducted. Results: High PTPN12 expression was seen in 39.2% of TNBC and 60.2 % of non-TNBC cases. Low PTPN12 expression was associated with lymph node status (p = 0.002) and distant metastatic relapse (p = 0.002) in TNBC patients. Similarly, low PTPN12 expression in non-TNBC patients was significantly correlated with lymph node status (p = 0.002), stage (p = 0.002) and distant metastatic relapse (p = 0.039). The high PTPN12 expression group was associated with longer DFS and OS compared with low PTPN12 expression group only in TNBC cases (p = 0.005, p = 0.015), according to univariate Cox regression analysis. Conclusion: These findings provide evidence that low expression of PTPN12 is associated with worse prognosis and may be used as a potential prognostic biomarker in TNBC patients.

Degradation of Chlorinated Organic Compounds by Zero Valent Metals and an Electron carrier

  • Kim, Young-Hun
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2001.04a
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    • pp.53-56
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    • 2001
  • The degradation of tetrachloroethene (PCE) and trichloroethene (TCE) by vitamin B$_{12}$, an electron mediator was examined when zero valent metals (ZVMs) were used as built electron donors. Dechlorination of PCE and TCE by iron and zinc in the presence of vitamin B$_{12}$ showed that the zinc and vitamin B$_{12}$ combination greatly enhances the reaction rates for both PCE and TCE, but iron and vitamin B$_{12}$ result in an increase in reactivity only for PCE degradation, not for TCE degradation in comparing with meta]s only. This result indicates vitamin B$_{12}$(I) Is active towards both PCE and TCE degradation while vitamin B$_{12}$(II) is active towards both PCE. Calculated activation energies for the dechlorination of PCE in the presence of Vitamin B$_{12}$ showed that vitamin B$_{12}$ lowered the activation energy about 40-60 kJ/㏖ for the both metals.the both metals.

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