• Journal of the Institute of Electronics and Information Engineers
• /
• v.53 no.5
• /
• pp.132-140
• /
• 2016

In this paper, we propose boundary-preserving stereo matching method based on adaptive disparity adjustment using confidence region detection. To find the initial disparity map, we compute data cost using the color space (CIE Lab) combined with the gradient space and apply double cost aggregation. We perform left/right consistency checking to sort out the mismatched region. This consistency check typically fails for occluded and mismatched pixels. We mark a pixel in the left disparity map as "inconsistent", if the disparity value of its counterpart pixel differs by a value larger than one pixel. In order to distinguish errors caused by the disparity discontinuity, we first detect the confidence map using the Mean-shift segmentation in the initial disparity map. Using this confidence map, we then adjust the disparity map to reduce the errors in initial disparity map. Experimental results demonstrate that the proposed method produces higher quality disparity maps by successfully preserving disparity discontinuities compared to existing methods.

• Journal of Food Hygiene and Safety
• /
• v.31 no.1
• /
• pp.28-35
• /
• 2016

Species identification of animal tissues in meat products is an important issue to protect the consumer from illegal and/or undesirable adulteration; for economic, religious and health reasons. In this reason, accurate analytical methods are needed for the labeling of meat products with requiring simple and fast procedure. Recently, applications of PCR in food analysis have been increased because of their simplicity, specificity and sensitivity. Therefore, in this study, a multiplex PCR assay was developed for the simultaneous identification of eight species of cow, pig, chicken, duck, goat, sheep, horse and turkey from raw meats. The primers were designed in different regions of mitochondrial 16S RNA after alignment of the available sequences in the GenBank database. Two multiplex primer sets were designed as Set 1 (cow, pig, chicken, duck) and Set 2 (goat, sheep, horse, turkey), respectively. Total 274 samples from cow (n = 55), pig (n = 30), chicken (n=30), and duck (n = 30), goat (n = 40), sheep (n = 33), horse (n = 41), and turkey (n = 15) were tested. The primers generated specific fragments of 94, 192, 279, 477 bp (pig, chicken, cow, duck), 670, 271, 152, 469 bp (goat, sheep, horse, turkey) lengths for eight species, respectively. The animal species specificity was 100% in all eight samples in the multiplex PCR assay. The detection limit of the multiplex PCR assay showed from 100 fg to 1 pg of template DNA from extracted from raw meats. When applying multiplex PCR assays to sample from pork/beef and pork/chicken, beef/chicken tested raw mixed meats and heat-treated ($83^{\circ}C$ for 30min, $100^{\circ}C$ for 20min, and $121^{\circ}C$ for 10min) mixtures, detection limit was 0.1% level beef, pork and pork in beef and chicken in pork and 1.0% level pork in chicken. This study suggest that the developed multiplex PCR assay can be used for rapid and simultaneous species identification of cow, pig, chicken, duck, goat, sheep, horse and turkey from meats.