• Nutrition Research and Practice
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• v.8 no.3
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• pp.278-283
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• 2014

BACKGROUND/OBJECTIVES: Due to its beneficial health effects, use of buckwheat has shown a continuous increase, and concerns regarding the allergic property of buckwheat have also increased. This study was conducted for evaluation of the hydrolytic effects of seven commercial proteases on buckwheat allergens and its allergenicity. MATERIALS/METHODS: Extracted buckwheat protein was hydrolyzed by seven proteolytic enzymes at individual optimum temperature and pH for four hours. Analysis was then performed using SDS-PAGE, immunoblotting, and competitive inhibition ELISA (ciELISA) with rabbit antiserum to buckwheat protein, and direct ELISA with pooled serum of 21 buckwheat-sensitive patients. RESULTS: Alkaline protease, classified as serine peptidase, was most effective in reducing allergenicity of buckwheat protein. It caused decomposition of the whole buckwheat protein, as shown on SDS-PAGE, and results of immunoblotting showed that the rabbit antiserum to buckwheat protein no longer recognized it as an antigen. Allergenicity showed a decrease of more than 50% when pooled serum of patients was used in ELISA. Two proteolytic enzymes from Aspergillus sp. could not hydrolyze buckwheat allergens effectively, and the allergenicity even appeared to increase. CONCLUSIONS: Serine-type peptidases appeared to show a relatively effective reduction of buckwheat allergenicity. However, the antigenicity measured using rabbit antiserum did not correspond to the allergenicity measured using sera from human patients. Production of less allergenic buckwheat protein may be possible using enzymatic hydrolysis.

• Journal of Food Hygiene and Safety
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• v.7 no.4
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• pp.137-142
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• 1992

The distribution of V. parahaemolyticus was surveyed from various kinds of shells in Kunsan Bay from July to September, 1987. 1be morphological, biochemical and serological characteristics of the isolated strains were studied. The results were as follows: 1. 41 strains were isolated from 1,350 specimens of shells (Crassostrea gigas, Tapes philippinarum, Meretrix Iusoria) 2. The isolation rates of V. parahaemolytieus were 3% in July, 3.8% in August, and 2.2% in September, respectively. 3. V. parahaemolytkus was more frequently isolated from Kunsan (20 strains) than Bideukgi (12 strains) and Gae Hwa-do (9 strains). 4. V. parahaemolyticus was more frequently isolated from C gigas (23 strains) than other shells. 5. Kanagawa hemolysis reactions were all negative. 6. 6 Strains positive to K pooled antiserum included K IV, K V, K VI and K vn type.