• Nutrition Research and Practice
• /
• 제8권3호
• /
• pp.278-283
• /
• 2014

BACKGROUND/OBJECTIVES: Due to its beneficial health effects, use of buckwheat has shown a continuous increase, and concerns regarding the allergic property of buckwheat have also increased. This study was conducted for evaluation of the hydrolytic effects of seven commercial proteases on buckwheat allergens and its allergenicity. MATERIALS/METHODS: Extracted buckwheat protein was hydrolyzed by seven proteolytic enzymes at individual optimum temperature and pH for four hours. Analysis was then performed using SDS-PAGE, immunoblotting, and competitive inhibition ELISA (ciELISA) with rabbit antiserum to buckwheat protein, and direct ELISA with pooled serum of 21 buckwheat-sensitive patients. RESULTS: Alkaline protease, classified as serine peptidase, was most effective in reducing allergenicity of buckwheat protein. It caused decomposition of the whole buckwheat protein, as shown on SDS-PAGE, and results of immunoblotting showed that the rabbit antiserum to buckwheat protein no longer recognized it as an antigen. Allergenicity showed a decrease of more than 50% when pooled serum of patients was used in ELISA. Two proteolytic enzymes from Aspergillus sp. could not hydrolyze buckwheat allergens effectively, and the allergenicity even appeared to increase. CONCLUSIONS: Serine-type peptidases appeared to show a relatively effective reduction of buckwheat allergenicity. However, the antigenicity measured using rabbit antiserum did not correspond to the allergenicity measured using sera from human patients. Production of less allergenic buckwheat protein may be possible using enzymatic hydrolysis.

• 한국식품위생안전성학회지
• /
• 제7권4호
• /
• pp.137-142
• /
• 1992

1987년 7월부터 9월에 걸쳐 군산만에서 서식하는 세 종류의 패류로부터 장염비브리오의 분포를 조사하여 그 분리 균주에 대한 형태학적, 생화학적 및 혈청학적 실험으로 얻은 결과는 다음과 같다. 1. 총 1,350 시료의 패류로부터 41균주(3%)의 장염비브리오가 분리되었다. 2.장염비브리오의 분리율은 각각 7월 3%, 8월에 3.8% 그리고 9월에 2.2%이었다. 3. 장염비브리오의 비득지(12균주)와 계화도 (9균주)보다 군산 (20균주)에서 빈도가 높게 분리되었다 . 4. 장염비브리오는 다른 패류부터 굴에서 더 높은 빈도로 분리되었다. 5. Kanagawa 용혈반응은 전 균주가 음성이었다. 6. K 다가 항 혈청에 양성을 보인 6균주가 K IV와 VII인자형이 각각 1주 , K V와 VI 인자형이 각각 2주였다.